農漁村 私學의 財政的 危機와 對替

成昌根 대한사립학교장회(구 대한사립중고등학교장회) 1976 私學 Vol.1 No.-

Nucleotide Sequences of Bovine Ornithine Decarboxylase mRNA

성창근,Sung, Chang,Sparks, Robert Institute of Agricultural Science 1993 Korean Journal of Agricultural Science Vol.20 No.2

Ornithine Decarboxylase는 동물세포에 있어서 polyamines을 생합성하는 반응의 율속 단계에 있는 효소이다. 세포의 성장기간 동안에 이 효소는 mRNA와 단백질 수준에서 급격한 변화를 일으키며 조절된다. 이와 같은 polyamines농도의 급변화를 분자수준에서 탐구하기 위하여 ODC 특이 cDNA colne이 cDNA libray에서 분리되었다. cDNA의 clone이 이루어진 부분은 open reading frame과 3'-noncording region, 그리고 poly A의 tail이 있는 부분으로서 각각 456, 348, 14개의 nucleotides로서 구성되어 있었다. 인간, mouse, rat, hamster로부터 같은 지역내 추론된 C-말단으로부터의 아미노산의 비교는 단백질 상동성에 88%이상을 보였다. 이와같은 아미노산 염기순서에 있어서의 잘 보존된 특징은 ODC가 세포성정과 분화에 있어서 중요한 역할이 있음을 잘 나타내고 있다. Ornithine decarboxylase is the first and rate limiting enzyme in the biosynthesis of polyamines in mammalian cells. During cell growth the enzyme is regulated by rapid changes in the level of its mRNA and protein. To explore the molecular basis of these changes, ODC-specific complementary DNA (cDNA) clones were isolated from a bovine cDNA library. This region of the cDNA contained a portion of the open reading frame, a 3'noncoding region, and a poly-A tail of 456, 348, and 14 nucleotides, respectively. A comparison of the deduced sequence of the carboxyl terminal 151 amino acids of ODC with amino acid sequences in the same region of the enzyme from human, mouse, rat, and hamster showed greater than 88% identity in these proteins. The highly conserved nature of the amino acid sequences may be related to the important role of ODC in cell growth and differentiation.

항생물질을 생산하는 곰팡이의 분리 및 그 생산조건(제1보)

성창근,김찬조 충남대학교 농업과학연구소 1984 農業技術硏究報告 Vol.11 No.1

The followings are the results obtained from the investigations of isolation and cultural conditions of a fungus producing antibiotic from humus, spring soil, paddy field and etc. 1. Among fungi which were capable of antimicrobial substance producing at the 45C, Strain H-3 was selected as test strain because of its efficiency in antimicrobial substance producing. 2. Strain H-3 was identified as a Aapergullus fumigatus by the mophological properties. 3. The composition of optimum media for the production of antimicrobial substance were followed; glucose 20g, NaNCO₃ 3g. K₂HPO₄ 0.5g, CaCl₂ 0.5g, MgSO₄ 0.5g. FeSO₄ 0.005g. 4. Optimum culture conditions were founded to be pH 5.0, 40℃ respectively. 5. The maximum anti microbial substance producing obtained in this studs was showed 14mm of clear zone after 72 hrs incubation under the optimum conditions. 6. AF-c substance was active against Gram-positive, Cram-negative bacteria, and yeast but not mold in its biological properties.

수학적 창의성 계발을 위한 과제와 수업 방향 탐색

성창근,박성선 한국초등수학교육학회 2012 한국초등수학교육학회지 Vol.16 No.2

본 연구는 창의성이 발현되는 인지적 과정이 무엇인지에 대한 관점을 이론적으로 고찰한 후, 이를 토대로 수학적 창의성을 계발하고 측정하는데 바람직한 과제와 수업 방향을 제시하는 것을 목적으로 한다. 먼저, 창의성에 대한 영역-특수적 관점과 영역-일반적 관점을 이론적으로 고찰하였다. 창의성 발현에 대한 이 두 관점은 이론적 논의에 그치지 않고 수학적 창의성을 계발하고 신장시키기 위해 고안된 과제와 프로그램에 영향을 미친다. 창의성에 대한 교육학적 고찰에서는 수학적 창의성을 검사하고 계발하기 위한 과제와 수업 프로그램이 구비해야할 조건을 이론적으로 탐색한 후, 이를 바탕으로 실제 수학 수업에서 활용가능한 과제와 수업 사례를 제시하였다. 이 연구의 핵심적인 결론은 창의성의 발현되는 과정에 대한 연구는 수학적 창의성 연구의 핵심이 되어야 하며, 아울러 확산적 사고는 수학적 창의성 계발을 위한 필요조건이지만 충분조건은 될 수 없으므로, 수학적 창의성을 계발하기 위해서는 일반화, 추상화 등 다양한 수학적 추론과 수학적 지식을 고려할 필요가 있다.

Detecting Cerebral Microbleeds on Susceptibility-Weighted MR Imaging: Comparison with the Conventional MR Imaging Sequences

성창근,변준수,유현,나형일,서재승,김기현 대한영상의학회 2009 대한영상의학회지 Vol.60 No.2

Purpose: The purpose of our study was to compare the susceptibility-weighted imaging (SWI) with the conventional MR imaging sequences for their ability to detect cerebral microbleeds. Materials and Methods: We studied 17 patients (9 men and 8 women, mean age: 58 years) with microbleeds. The gradient-recalled echo T2*-weighted imaging (GRE), the T2-weighted imaging (T2WI), the fluid-attenuated inversion recovery (FLAIR) imaging and the SWI were obtained from all the subjects. The number of microbleeds was counted on each MR imaging sequence. We also compared the number of detected microbleeds (%) of each imaging technique with that of SWI being 100%. The Friedman ANOVA Test was used for statistical analysis and a p value of less than 0.05 was considered statistically significant. Results: The number of detected mircobleeds on each imaging technique (%, mean ± standard deviation), as compared to that detected on SWI as 100%, was as follows: GRE vs. SWI (64.9 ± 19.3 vs. 100, p =0.001), T2WI vs. GRE (16.9 ± 17.7 vs. 64.9 ± 19.3, p = 0.001), and T2WI vs. FLAIR (16.9 ± 17.7 vs. 13.8 ± 17.7, p = 0.027). Conclusion: SWI demonstrates a significantly higher sensitivity for detecting microbleeds as compared to that of GRE and the other conventional MR imaging sequences.

轉換期의 陣痛 - 敎育法 改正을 앞두고 -

成昌根 대한사립학교장회(구 대한사립중고등학교장회) 1989 私學 Vol.48 No.-

마늘 추출물의 벼 키다리병에 대한 종자소독 효과

성창근,최지호 충남대학교 농업과학연구소 2004 농업과학연구 Vol.31 No.1

Bakanae Disease is an abnormal plant growth caused by Gibberella fujikuroi. The infected plants several inches taller than normal plants in seedbed and field. To control bakanae disease, we made a seed disinfectant from garlic extract. And we isolated allicin(C_(6)H_(10)OS_(2)) from garlic extract and the content was 0.62%. In order to develop efftive seed disinfectant from Garlic extract, we compared it with three seed disinfectants(benomyl, prochloraz, fludioxonil). Prochloraz, fludioxonil and Garlic wettable powder controlled Bakanae disease of rice very well, while benomyl did not suppress the disease enough. Water temperature was turned to be an important factor for controlling the disease by treating seed disinfectants. It was confirmed that the seed disinfectant from garlic extract can be used as non-toxic agricultural medicines.

구기자의 당, 아미노산, 지방산, 무기물 조성

성창근,오만진,김찬조 충남대학교 농업과학연구소 1994 농업과학연구 Vol.21 No.1

Gugija, Lycium fructus, grown widely in Korea has long been used as a chinese herbal medicine. For utilization as a new resource in food industry, and as a fundamental study for processing various gugija products, the chemical compositions of Lycium fructus were studied. Three different kinds of free sugar were analyzed by HPLC, and the composions were glucose, 5.6% : fructose, 5.81% : sucrose, 4,39%. respectively. Free amino acids isolated as a major components in dried Lycium fructus were aspartic acid/asparagine, and glutamic acid/glutamine. The major fatty acids were linoleic, oleic, and palmitic acids. and these composed about 82.98∼89.64% of total fatty acids. There are not much differences in contents of the major fatty acids among varieties in the fruits. Lycium fructus. The Ca^++, Fe^++ contents of chinese variety were higher than those of other fruit vanities studied.

Enhanced Lovastatin Production by Solid State Fermentation of Monascus ruber

성창근,Bao-Jun Xu,Qi-Jun Wang,Xiao -Qin Jia 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.1

The purpose of this study was to optimize the solid state cultivation of Monascus ruber on sterile rice. A single-level-multiple-factor and a single-factor-multiple-level experimental design were employed to determine the optimal medium constituents and to optimize carbon and nitrogen source concentrations for lovastatin production. Simultaneous quantitative analyses of the -hydroxyacid form and -hydroxylactone for of lovastatin were performed by the high performance liquid chromatography (HPLC) method with a UV photodiode-array (PDA) detector. The total lovastatin yield (4~6 mg/g, average of five repeats) was achieved by adding soybean powder, glycerol, sodium nitrate, and acetic acid at optimized levels after 14 days of fermentation. The maximal yield of lovastatin under the optimal composition of the medium increased by almost 2 times the yield observed prior to optimization. The experimental results also indicated that the -hydroxylactone form of lovastatin (LFL) and the -hydroxyacid form of lovastatin (AFL) simultaneously existed in solid state cultures of Monascus ruber, while the latter was the dominant form in the middle-late stage of continued fermentation. These results indicate that optimized culture conditions can be used for industrial production of lovastatin to obtain high yields.

미생물 ornithine decarboxylase와 polyamines에 관하여

성창근 한국미생물학회 1993 微生物과 産業 Vol.19 No.1

polyamine을 생합성하는 율속단계효소인 ornithine decarboxylase(ODC)는 미생물에서 많이 연구되어 왔으며 실제적으로 이 효소에 대한 연구는 세균에서 그 효시를 이루었다(1-5,19,35). 최근 들어서는 Escherichia coli(1-22), Saccharomyces cerevisiae (35-40), Neurospora crassa, Physarum polycephalum에서 보다 상세한 연구가 이루어졌는데, 본 고에서는 Escherichia coli, Saccharomyces cerveisiae에 관하여 좀 더 자세히 살펴보고자 한다. Polyamines의 연구나 ODC효소에 대한 연구는 미생물이 많이 이용되었는데, 이는 비교적 쉽게 세포배양이 되기도 하지만, ODC가 결손된 변이주를 만들기가 비교적 용이한 이유도 있었다.