전염성 췌장 괴저 바이러스의 어류세포에의 감염에 미치는 리소솜 친화성 약염기의 영향

박정우,하영칠,정가진 ( Jeong Woo Park,Yung Chil Hah,GajIn Jeong ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.4

We investigated the effect of ammonium chloride, which is known to affect lysosome-mediated viral internalization, on the infectious of fish cells, CHSE-214, with Infectious Pancreatic Necrosis Virus (IPNV). Progeny production of IPNV was delayed 6 to 8 hours in IPNV infected CHSE-214 cells by the addition of NH₄C1 at the final concentration of 20 mM. The multiplication of IPNV, however, was no longer inhibited when NH₄C1 was present only for the first 4 hours after viral infection. Thus the inhibitory effect of NH₄C1 seems to be on some other steps but internalization. For the inhibitory effect of NH₄C1, time of addition is not very important, but the length of treatment is rather critical. Ammonium chloride attenuated the synthesis of almost all the proteins in IPNV-infected CHSE-214 cells. Thus the primary effect of NH₄C1 on virus-infected host cells is to inhibit the synthesis of proteins resulting in the delay of IPNV growth. Therefore, we conclude that IPNV penetration into CHSE-214 cells does not require the low-pH dependent activity of lysosome.

국내분리 Aujeszky's disease virus의 실험적 감염 자돈에 대한 바이러스학적 연구

박정우,전무형,안수환,Park, Jeong-woo,Jun, Moo-hyung,An, Soo-hwan 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.2

To investigate the etiology, pathogenicity and virological properties of NYJ-1-87 strain of Aujeszky's disease virus (ADV) that was isolated from the diseased piglet in Korea, the virus at $10^{6.0}TCID_{50}/0.1ml$ was inoculated intranasally and subcutaneously into 30 to 35 days-old piglets. Results obtained through the experiments were summarized as follows. 1. Ten of the infected piglets were clinically observed for 15 days. On the 2nd day post-inoculation(pi), the signs of pyrexia, anorexia and convulsion were noted. On the 4th to 7th days pi, nervous signs of incoordination and intermittent spasm were shown in the most of piglets, and one out of 5 piglets infected intranasally was died with severe nervous signs at the 7th day pi. The signs became relieved on the 8th day pi and all of remainder were completely recovered on the 13th to 14th days pi. 2. In hematological study, prominent decrease in the number of total leukocyte and lymphocyte was shown in the ADV-infected piglets on the 6th day pi. On the 8th day pi, the cell numbers were slightly increased and returned to normal level on the 10th day pi. 3. Viral excretion of the ADV-inoculated piglets was examined by swabbing of nasal and oral cavities, and rectal feces. During the periods of the 3rd to 11th days pi, the virus was excreted intermittently from nasal and oral cavities, and rectal feces. The nasal excretions were shown the highest virus concentration of $10^{5.2}TCID_{50}/0.1ml$ at the 5th day pi. 4. Recovery of the inoculated virus from various organs of the piglets that were died or experimentally slaughtered was attempted, and the virus was isolated from the tissues of brain and tonsil by the cultured cell-inoculation method. The highest recovery rate was noted in the tonsil. By indirect immunofluorescence antibody assay using ADV-monoclonal antibody, the viral antigens were detected in tissues of spleen and liver as well as brain and tonsil on the 7th to 9th days pi. The virus was not isolated from blood and the tissues of lung and kidney throughout the experiments. 5. Titers of virus neutralizing antibody in the piglets experimentally infected with ADV became increased after the 6th to 9th days pi in both of intranasal and subcutaneous inoculation showing the highest titers of 64 to 128 on the 29th day pi. When the antibody levels were measured by radial immunodiffusion enzyme assay, the reactive diameter was enlarged to be positive after the 4th to 6th days pi in both of intranasal and subcutaneous inoculation showing the largest diameter of 13 to 14mm on the 29th day pi.

바위 자고새의 흑두병 발생예

박정우,전무형,조성환,조우영,Park, Jeong-woo,Jun, Moo-hyung,Cho, Sung-whan,Cho, Woo-young 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.2

The diseased Chuckar partridges that were raised at the wild bird-raising farms located in Ockchun, Chungbuk province, were submitted for diagnosis in the laboratory in July. 1990. The diseased partridges aged 3~4 weeks old were shown clinical signs such as anorexia, depression, white-colored or transparent-watery diarrhea and dropping of the head and wings. At necropsy, major gross lesions were found in the livers, ceca and lungs. Histopathologically, numerous histomonads were observed in the necrotic tissues of the livers and ceca. In the cecal specimens, it was found that the ameboid histomonads have flagellum and the size of 10~20 micrometer. The case was confirmed as histomoniasis due to the infection of Histomonas meleagridis.

인산 및 수증기 활성화에 의한 대나무 활성탄 제조 및 특성 연구

박정우,리황부,오창호,김승수,Park, Jeong-Woo,Ly, Hoang Vu,Oh, Changho,Kim, Seung-Soo 한국청정기술학회 2019 청정기술 Vol.25 No.2

Bamboo is an evergreen perennial plant, and it is known as one of the most productive and fastest-growing plants in the world. It grows quickly in moderate climates with only moderate water and fertilizer. Traditionally in Asia, bamboo is used for building materials, as a food source, and as versatile raw materials. Bamboo as a biomass feedstock can be transformed to prepare activated carbon using the thermal treatment of pyrolysis. The effect of process variables such as carbonization temperature, activation temperature, activation time, the amount of steam, and the mixing ratio of phosphoric acid and bamboo were systematically investigated to optimize the preparation conditions. Steam activation was proceeded after carbonization with a vapor flow rate of $0.8{\sim}1.8mL-H_2O\;g-char^{-1}\;h^{-1}$ and activation time of 1 ~ 3 h at $700{\sim}900^{\circ}C$. Carbon yield and surface area reached 2.04 ~ 20.59 wt% and $499.17{\sim}1074.04m^2\;g^{-1}$, respectively, with a steam flow rate of $1.4mL-H_2O\;g-char^{-1}\;h^{-1}$ for 2 h. Also, the carbon yield and surface area were 24.67 wt% and $1389.59m^2\;g^{-1}$, respectively, when the bamboo and phosphoric acid were mixed in a 1:1 weight ratio ($700^{\circ}C$, 2 h, $1.4mL-H_2O\;g-char^{-1}\;h^{-1}$). The adsorption of methylene blue into the bamboo activated carbon was studied based on pseudo first order and second order kinetics models. The adsorption kinetics were found to follow the pseudo second order model, which is governed by chemisorption. 대나무는 지구상에 존재하는 식물 중 적절한 기후와 토양조건에서 생산성이 가장 높고, 성장속도가 가장 빠른 다년생 식물로 알려져 있다. 전통적으로 아시아에서 대나무는 음식, 건축 및 다양한 재료로 활용되고 있다. 바이오매스 자원으로 대나무는 열분해과정을 거쳐 활성탄으로 제조될 수 있다. 본 연구에서는 탄화온도, 활성화 온도, 시간, 수증기의 양, 그리고 인산의 양 등을 변화에 따른 최적의 대나무 활성탄 제조 연구를 수행하였다. 대나무 탄화 후 수증기 활성화를 위해 $700{\sim}900^{\circ}C$의 온도, $0.8{\sim}1.8mL-H_2O\;g-char^{-1}\;h^{-1}$ 수증기 유량 범위에서 1 ~ 3 h 동안 활성화를 진행하였다. 수증기 유량을 $1.4mL-H_2O\;g-char^{-1}\;h^{-1}$으로 2 h 동안 실험한 결과 활성탄 수율과 비표면적은 각각 2.04 ~ 20.59 wt%, $499.17{\sim}1074.04m^2\;g^{-1}$의 값이 나왔다. 대나무와 인산의 질량비를 1:1로 혼합한 후 $700^{\circ}C$에서 유량 $1.4mL-H_2O\;g-char^{-1}\;h^{-1}$ 속도로 2 h 동안 활성화를 진행한 결과 활성탄 수율과 비표면적은 각각 24.67 wt%, $1389.59m^2\;g^{-1}$의 값이 나타냈다. 제조된 대나무 활성탄을 대상으로 메틸렌블루 흡착 실험을 통해 유사 1차, 2차 속도식 모델을 적용하였으며, 화학적 흡착을 의미하는 유사 2차 속도식에 따랐다.

기술표준동향 - ISO 26000 사회적 책임 (Social Responsibility) 국제표준동향

박정우,Park, Jeong-Woo 기술표준원 2009 기술표준 Vol.89 No.-

한방 치료와 코어 운동을 병행한 퇴행성 슬관절염 환자 1례에 대한 증례보고

박정우,정수현,Park, Jung-Woo,Jeong, Su-Hyeon 척추신경추나의학회 2013 척추신경추나의학회지 Vol.8 No.2

Objectives : The objective of this study is to report the effect of Traditional Korean medical therapy and core exercise on osteoarthritis of knee joint by observing a clinical case study. Methods : Patient with a diagnosis of osteoarthritis of knee joint, was treated by acupuncture, herb medicine, physical theraphy and core exercise. Results : After treatment, Visual Analogue Scale(VAS), Korean version of the Western Ontario and McMaster Universities(KWOMAC), Pain Rating Score(PRS) were reduced from 5 to 2, from 25 to 16, from 55 to 27, and CENTAUR test results were improved. Conclusions : This study showed that Traditional Korean medical therapy and core exercise has meaningful effect on osteoarthritis of knee joint, and more researches should be followed.

IHNV (Infectious Hematopoietic Necrosis Virus): 과거, 현재, 그리고 미래

박정우 ( Jeong Woo Park ),조미영 ( Miyoung Cho ),이언화 ( Unn Hwa Lee ),최혜승 ( Hye Sung Choi ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.5

A global increase in fish consumption has led to a rapid expansion of aquaculture production, which has been linked to enhancing the spread of infectious diseases. Viral diseases can cause high mortality in many cultured fish species, posing a serious threat to the aquaculture industry. Infectious hematopoietic necrosis virus (IHNV) is one of the primary threats to aquacultured salmonid species, causing huge economic losses. Since the first report in cultured sockeye salmon Oncorhynchus nerka during the 1950s in North America, IHNV has spread to other regions, including Europe, Asia, South America, and Africa by transportation of infected fish and eggs, causing disease and increasing mortality in a wide variety of salmonid species. Here, we review existing information relevant to IHNV: its phylogenetic characteristics, origin, infection history, virulence determinants, susceptible hosts, vectors, and vaccine development. This review also addresses a possible cross-species transmission of IHNV to a new host, olive flounder Paralichthys olivaceus, a cultured fish of economic importance in East Asian countries.

망막병증을 동반하지 않은 초기 제2형 당뇨병 환자에서 망막혈관 직경의 변화

박정우(Jeong Woo Park),배정훈(Jeong Hun Bae),송수정(Su Jeong Song),김준모(Joon Mo Kim) 대한안과학회 2022 대한안과학회지 Vol.63 No.1

목적: 망막병증이 동반되지 않은 초기 제2형 당뇨병 환자에서 망막혈관의 직경을 측정하고, 정상안과 비교하여 그 변화를 알아보고자 하였다. 대상과 방법: 제2형 당뇨병으로 진단받은 지 10년 이내의 환자 85명과 나이와 성별이 유사한 건강한 대조군 48명의 의무기록을 후향적으로 분석하였다. 모든 환자들은 산동 후 안저검사를 통해 당뇨망막병증이 동반되지 않음을 확인하였고, 안저카메라를 이용하여 안저촬영을 시행하였다. 망막혈관의 직경을 반자동으로 측정해주는 프로그램을 이용하여 중심망막동맥대응치, 중심망막정맥대응치, 동정맥비 값을 구하였다. 대조군도 환자군과 같은 방법으로 검사를 시행하고, 두 군을 비교 분석하였다. 결과: 환자군과 대조군의 중심망막동맥대응치는 각각 149.92 ± 21.29 μm, 144.85 ± 17.14 μm로 두 군 간에 유의한 차이가 없었고(p=0.137), 중심망막정맥대응치도 환자군과 대조군에서 각각 195.85 ± 22.94 μm, 196.69 ± 18.43 μm로 차이가 없었다(p=0.829). 그러나, 동정맥비는 환자군에서 0.768 ± 0.088이었고, 대조군은 0.737 ± 0.061로 두 군 사이에 유의한 차이가 있었다(p=0.031). 환자군에서 동정맥비는 당화혈색소와 유의한 상관관계가 있었으나(r=0.325, p=0.006), 체질량지수와는 관련이 없었다(p=0.478). 결론: 시신경유두 주위 동정맥비의 상승은 제2형 당뇨병 환자에서 관찰되는 망막혈관의 초기 변화로 생각되며, 이는 혈당의 증가와 밀접한 관련이 있다. Purpose: To evaluate the retinal vascular caliber in early type 2 diabetic patients without retinopathy. Methods: This retrospective study reviewed the medical records of 85 patients who were diagnosed with type 2 diabetes (age, 10-48 years) and sex-matched healthy controls. Dilated fundus examinations were performed to confirm the absence of diabetic retinopathy. Fundus photographs were acquired and analyzed using software that semi-automatically measured retinal vascular caliber. Central retinal arteriolar equivalent (CRAE), central retinal venular equivalent (CRVE), and arteriovenous ratio (AVR) were determined using the revised Parr-Hubbard formula. Healthy controls were examined using the same approach. Results: There were no significant differences between patients and controls in terms of CRAE (149.92 ± 21.29 μm and 144.85 ± 17.14 μm, respectively; p = 0.137) or CRVE (195.85 ± 22.94 μm and 196.69 ± 18.43 μm, respectively; p = 0.829). However, AVR was significantly higher in the patient group (0.768 ± 0.088 vs. 0.737 ± 0.061; p = 0.031). In the patient group, AVR was significantly correlated with hemoglobin A1c (r = 0.325, p = 0.006), but not with body mass index (p = 0.478). Conclusions: An increase in peripapillary AVR may be an early feature of retinal vessels observed in patients with type 2 diabetes, which is closely related to elevated blood sugar.

AFM 기반 액중 Tribo nanolithography 에서의 마스크 층 내식각성에 관한 연구

박정우(Jeong Woo Park),이득우(Deug Woo Lee) 한국정밀공학회 2005 한국정밀공학회 학술발표대회 논문집 Vol.2005 No.10월

Etch resistance of mask layer on silicon substrate modified by AFM-based Tribo-Nanolithography (TNL) in Aqueous Solution in an aqueous solution was demonstrated. TNL consists of sequential processes, nano-scratching and wet chemical etching. The simple scratching can form a mask layer on the silicon substrate, which acting as an etching mask. For TNL, a specially designed cantilever with diamond tip, allowing the formation of mask layer on silicon substrate easily by a simple scratching process, has been applied instead of conventional silicon cantilever for scanning. This study demonstrates how the TNL parameters can affect the etch resistance of mask layer, hence introducing a new process of AFM-based maskless nanolithography in aqueous solution.

돼지 분변 유래 PORCINE ENTEROVIRUS의 물리학적 특성 및 병원성에 관한 연구

박정우 ( Jeong Woo Park ),이종인 ( Jong In Lee ),신용호 ( Yong Ho Shin ),조우영 ( Woo Young Cho ),최윤식 ( Yun Shic Choi ) 한국가축위생학회 1991 韓國家畜衛生學會誌 Vol.14 No.2

28 porcine enteroviruses were isolated from 86 pig-feces of 9 swine farms located in souti region, Chung-buk, from March to September 1990. Physico-chemical properties and pathogenicity of isolates were investigated. Results obtained throughout experiments are summarized as follows. According to the age, weanlings(40-90 days), sucklings(10-30 days) and adult pigs(6 months over) showed the isolation rate of 67%, 8% and 4%, respectively. By physico-chemical tests, YD-90 /22, YD-90/43 and YD-90 /64 strains were found to be ether, chloroform and PH stable. Nucleic acid test suggests the virus to have a DNA genome. Most of the isolates were not evident of hemagglutinin using erythrocytes from various mammalian & avian. 22 strains among the isolates were shown CPE type I and the remainders were CPE type Ⅱ. 3 strains among isolates of CPE type 1 strains were neutralized with high titers to serotype 2 antiserum. Ir. the study on virus growth curve in PK 15 cells, YD-90 /22, YD-90/43 and YD-90 /64 strains showed the maximum infectivity titers(106.0-106.5 TCID50 /me) at 4 days post inoculation(PI). When 30 day-old commercial piglets were inoculated only intraoral route with the YD-90/22 strain at 106.0 TCID50/m, piglets not showed the symptoms. But piglets inoculated by intramuscie route, intraoral and intramuscie route after pretreat with dexamethasone(2.5mg /kg) for 5 days were shown the symptoms of anorexia, diarrhea, pyrexia and ataxia at 4th 6th days PT. The viral reisolation in the virus-inoculated piglets was examined from feces. The viruses were recovered intermittently from 2nd to 16th day P1 and at 4th-6th day P1, all piglets excreted viruses.