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        전염성 췌장 괴저 바이러스의 어류세포에의 감염에 미치는 리소솜 친화성 약염기의 영향

        박정우,하영칠,정가진 ( Jeong Woo Park,Yung Chil Hah,GajIn Jeong ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.4

        We investigated the effect of ammonium chloride, which is known to affect lysosome-mediated viral internalization, on the infectious of fish cells, CHSE-214, with Infectious Pancreatic Necrosis Virus (IPNV). Progeny production of IPNV was delayed 6 to 8 hours in IPNV infected CHSE-214 cells by the addition of NH₄C1 at the final concentration of 20 mM. The multiplication of IPNV, however, was no longer inhibited when NH₄C1 was present only for the first 4 hours after viral infection. Thus the inhibitory effect of NH₄C1 seems to be on some other steps but internalization. For the inhibitory effect of NH₄C1, time of addition is not very important, but the length of treatment is rather critical. Ammonium chloride attenuated the synthesis of almost all the proteins in IPNV-infected CHSE-214 cells. Thus the primary effect of NH₄C1 on virus-infected host cells is to inhibit the synthesis of proteins resulting in the delay of IPNV growth. Therefore, we conclude that IPNV penetration into CHSE-214 cells does not require the low-pH dependent activity of lysosome.

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