Virus 에 감염된 대추나무의 이병엽과 (罹炳葉) 건전엽에 (健全葉) 있어서의 유리 (遊離) amino 산의 정성적 비교

홍순우(Soon Woo Hong),하영칠(Yung Chil Hah) 한국식물학회 1961 Journal of Plant Biology Vol.4 No.1

Hong, Soon-Wooe and Yung-Chil, Hah (Dept. of Botany, Seoul National University, Seoul, Kerea.) A compantive investigation of free amino acids in healthy and virus diseased Chinese date tree. Kor. Jour. Bot. 4 (1)9~12 1961 : A comparative investigation of free amino acids content in healthy check and virus diseased leaves of Chinese date tree, Zizyphus jujuqa Mill var. inermis Rhed, was carried out by authors throughout the growing season of 1959 and 1960 from June to October. The methods of qualitative analysis of free amino acids applied in this experiment is followed by Moore and Stein.^(1.2). free amino acids determined in this experiment are shown in Fig. 1 and Table 1. As the figure and the table are shown, three more amino acids such as glutarmine, asparagine and histidine are detected in the diseased material. The additiolal amino acids which are known as diamines in diseased leaves are conspicuous. It is presumed that the diamine might be increased by the self-reproduoion of the virus in cooporation with certain enzymes which are carrying out the protein metabolism in the host protoplast in contrast with the healthy checks which is carrying out normal protein metabolism,^(3.4.5). From the histological point of view, the facts of phloem degeneration or necrosis in diseased leaves, it seems to interrupt to move free amino acids from roots to leaves and it possibly takes place an excessive productitn of NH_3 which is diaminated by the metabolism of nitrogen compounds in such conditioned leaves.^(6.8) Therefore, it is also presumed that additional diamino acids are accumulated in diseased leaves. There are no change of amino acids in both materials of this plant throughout the growing season qualitatively, and this result agrees with the paper of Knight.^9

Factor analysis of Water Quality and Ecosystem in Jinhae Bay

홍순우,하영칠,안태석 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

진해만의 6개 정점의 표·지층에서 1976년 7월부터 1982년 12월까지 물리화학적, 생물학적인 환경요소를 65회에 걸쳐 조사하였다. 이들 자료를 통계학적인 분석을 통하여 진해만의 수질및 생태계에 영향을 미치는 요인을 분석하였다. 그 결과 행암만 내부에 위치한 정점 6 을 제외한 전 해역에서 식물성 플랑크톤의 이상증식이 주요한 요인으로 생태계의 44∼56%의 변화를 설명할 수 있었으며, 정점 6 에서는 영양염류가 중요한 요인이 됨을 알았다. 두번째 요인으로는 담수의 유입으로 볼 수 있었으며, 이 요인은 외해로 나갈수록 그 영향이 적어져, 정점 4 에서는 의미가 없었다. 질소의 유입도 각 정점에서 주요한 요인이었으며, 그 외에 유기물의 증가, 도시하수의 유입, 영양염류의 침전 및 미생물에 의한 염류의 재순환과 광합성이 진해만에서의 수질과 생태계를 지배하는 주요 요인으로 확인되었다. In Jinhae bay, the physicochemical, biological and ecological surveys were carried out 65times at six sites both insurface and bottom water from July 1976 to December 1982. Twenty one abiotic andbiotic parameters were determined on each sample. These data were subjected to factor analysis to scrutinize the effects of environmental conditions on water quality and ecosystem. The phytoplankto blooming was the major factor of the variance of the water quality and ecosystem in Jinhae bay. Fresh water inflow was second factor at site 1 and 6, and it was third factor in site 3 and 5. The nitrogen inflow was also play an improtant role, which was second factor at site 2, 3, 4 and 5, and third factor at site 1. Beside above factors, the increasing of organic materials, waste water in flow, precipitation and recycling of nutrients, upwelling of sediment, nutrient, salts and photosynthesis were also determined for the variation of water quality and ecosystem.

Isolation and Characterization of Bacterial Pathogens from Eels (Anguilla japonica) Cultured in Korea

Hah, Yung-Chil,Hong, Soon-Woo,Oh, Hee-Bok,Fryer, John L.,Rohovec, John S. The Microbiological Society of Korea 1984 미생물학회지 Vol.22 No.1

아산 양어장에서 양식되고 있는 뱀장어 (Anguilla japonica)로부터 22주의 병원성 세균을 분리하여 그 생화학적 성질, 혈청학적 관계, 금붕어에 대한 감염성 및 여러 항생물질에 대한 감수성을 시험하였다. 22균주중 14주 (64%)는 Edwardsiella tarda 5주 (23%)는 Aeromonas hydrophila, 기타 3균주(14%)는 Vibrio anguillarum으로 동정되었다. 3가지 분리균중에서 E. tarda는 동일한 혈청형으로 그 분리율 및 금붕어에 대한 감염성이 가장 높았다. 이에 따라 E. tarda가 조사된 양어장의 뱀장어에서 발생하는 세균성 질병의 주 병원체로 판명되었다. 세 분리균주의 균액을 수조에 풀어 금붕어에 접촉시켰을 때 그 감염율은 세 균주 모두 상대적으로 낮았다. 10주의 분리균을 택해 12가지 항생물질에 대해 감수성 검사를 한 결과 하나에서 여섯가지 약제에 대해 저항성을 나타냈으며, 그 중 tetracycline 유도체와 sulfisoxazole에 대한 저항성이 현저하였다. Twenty two cultures of pathogenic bacteria were isolated from cultured eels(Anguilla japonica) from Asan Hatchery. The bateria were characterized by their biochemical properties, serological relationships, infectivity to gold fish and susceptibility to various antimicrobial compounds. Fourteen of 22(64%) cultures were identified as Edwardsiella tarda, five (23%) as Aeromonas hydrophila and three (14%) as Vibro anguillarum. Edwardisiella tardo isolates proved to be the main cause of the disease in cultured eels. They were serologically homogeneous and their virulency to gold fish was higher than any of the other groups of bacteria tested. The virulence of 3 isolates were low in gold fish exposed to the bacteria by the waterborn route. Ten strains were tested for their susceptibility to 12 antimicrobial compounds and were resistant to from one to six drugs: in particular, tetracycline derivatives and sulfisoxazole.

The role of microbes in eutrophication

Yung-Chil Hah,Tae-Seok Ahn,Tae-Young Ahn,Ki-Seong Joh 江原大學校 附設 環境硏究所 1992 環境硏究 Vol.9 No.-

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Plenary Lectures ( 1998 . 11 . 12 - 17 ) : Strategy for Screening Novel Microorganisms

(Yung Chil Hah) 한국응용약물학회 1998 학술대회 Vol.1998 No.1

진해만의 수질과 생태계에 관한 요인분석에 관하여

하영칠,홍순우,안태석 한국물환경학회 1985 한국물환경학회지 Vol.1 No.1

진해만의 6개 정점의 표·저층에서 1976년 7월부터 1982년 12월까지 물리화학적 생물학적인 환경요소를 65회에 걸쳐 조사하였다. 이들 자료를 통계학적인 분석을 통하여 진해만의 수질 및 생태계에 영향을 미치는 요인을 분석 하였다. 그 결과 행암만 내부에 위치한 정점6을 제외한 전 해역에서 식물성 플랑크톤의 이상증식이 주요한 요인으로 생태계의 44∼56%의 변화를 설명할 수 있었으며, 정점6에서는 영양염류가 중요한 요인이 됨을 알았다. 두번째 요인으로는 담수의 유입으로 볼 수 있었으며. 이 요인은 외해로 나갈수록 그 영향이 적어져, 정점4에서는 의미가 없었다. 질소의 유입도 각 정점에서 주요한 요인이었으며, 그 외에 유기물의 증가 도시하수의 유입, 영향염류의 침전및 미생물에 의한 염류의 재순환과 광합성이 진해만에서의 수질과 생태계를 지배하는 주요 요인으로 확인되었다. In Jinhae bay, the physicochemical, biological and ecological surveys were carried out 65 times at six sites both in surface and bottom water from July 1976 to December 1982. Twenty one abiotic and biotic parameters were determined on each sample. These data were subjected to factor analysis to scrutinize the effects of environmental conditions on water quality and ecosystem. The phytoplankton blooming was the major factor of the variance of the water quality and ecosystem in Jinhae bay. Fresh water inflow was second factor at site 1 and 6, and it was third factor in site 3 and 5. The nitrogen inflow was also play an important role, which was second factor at site 2, 3, 4 and 5, and third factor at site 1. Beside above factors, the increasing of organic materials, waste water inflow, precipitation and recycling of nutrients, upwelling of sediment, nutrient salts and photosynthesis were also determined for the variation of water quality and ecosystem.

Microbial population in Han River estuary

Hong, Soon-Woo,Hah, Yung-Chil,Lee, Kwang-Woong The Microbiological Society of Korea 1968 미생물학회지 Vol.6 No.4

Examining the microbial populations in the Han River estuary, we conducted this experiment at six sites of the estuarine area, Chollyu-ri, Cholsan-ri, Wolgon-ri, Chogi-ri, Inhwa-ri, and Oepo-ri for 5 months since May 1967. From the results obtained it could be summarized as follows. 1) The salinity of the estuarine water increased in order of the distances from the base point of the Old Han River Bridge to every site of the estuary, and pH of the water, which were between 7. 3 and 8. 1, showing little difference each other in both the sites and dates of experiment. 2) The populations of the general bacteria and coliform group bacteria were highest at the site of Chollyu-ri, and it decreased with the downstreaming of the river water toward Oepo-ri site. As for fungi which have comparatively high tolerance to the salinity, its population was shown hi hest at Chogi-ri. 3) The relationship between the salinity and the number of the general bacteria have indicated that the incresed salinity reduced the growth rates of the bacteria. By this it can be assumed that the fresh-water bacteria decreased due to the sea water as well as its dilution effects. 4) The high pollution of the esturarine water was caused by the increases of inhabitants and water thrown by industries in Seoul and Kyung-In Industrial District as well as the excrements fertilized to the farms.

Virulence of Vibrio anguillarum induced by the low molecular weight secretory component is correlated to the hemolytic activity of RBCs from human and gold fish

Young Chool Choe,Yung Chil Hah,Ga Jin Jeong 한국미생물생명공학회(구 한국산업미생물학회) 1992 한국미생물생명공학회 학술발표초록집 Vol.1992 No.1

Foreword (1998 . 11 . 12 - 17 )

(Byung Hoon Han),(Yung Chil Hah) 한국응용약물학회 1998 학술대회 Vol.1998 No.1

微生物에 의한 石油內 硫黃分除去에 관한 硏究 : Ⅳ. 脫黃菌의 特性에 대한 生理學的 硏究 Ⅳ. Physiological Studies on Characters of Sulfur-oxidizing Bacteria

李敏載,河永七,李光雄,邊宇玄 서울대학교 1975 서울대학교 論文集 Vol.25 No.-

Serveral physiological characterstics of sulfur­oxidizing bacteria such as Thiobacillus thiooxidans and T. concretivorus are described. 1) Effect of pH on inhibitory function of pyruvate. The rate of oxygen uptake was reduced in accordance with decrease of pH. And on the other hand inhibition rate of pyruvate was more severe in lower pH than in higher pH. High concentration of hydrogen ion seemed to accelerate inhibitory function of pyruvate. Limiting concentration of pyruvate which absolutely inhibit oxygen uptake was lowered with time. In O time, 10^-2M of pyruvate absolutely inhibited oxygen uptake in pH 5. After 24 hours limiting concentration was lowered to 10^-3M. 2) Effect of organic compounds on the oxygen uptake of T. concretivorns. Effect of glucose, fructose, xylose, glutamate, succinate, malate, glycine, lactate, acetate, pyruvate, citrate, formate, and cis­aconitate on thiosulfate oxidation and availability of these compounds as sloe source of energy by T. concretivorus were observed. A 0.5% concentration of malate and glycine accelerated thiosulfate oxidation almost react somewhat inhibitory. Pyruvate and citrate inhibited thiosulfate oxidation. In thiosulfate­free medium, organic compounds except formate and pyruvate affected no significant influences on oxygen uptake. 3) Enzyme assay. Of enzymes concerned TCA cycle and glycolysis, T. concretivorus had most of those enzyme activities even though they were low. Activities of hexokinase and succinic dehydrogenase were somewhat high and that of aconitase was very low on the contrary. 4) Optimum condition of mass culture of sulfur oxidizing bacteria. In a large jar fermenter opimum conditions including agitations speed, volume of supplying gases, and constitution rate of oxygen and carbon dioxide, etc. were experimented. The sulfur­oxidier, T. thiooxidans was grown most effectively under the condition of autom­atically controlled pH of 1.0, and with the aeration of oxygen and carbon dioxide mixture (5 : 1) at the flow rate of 0.05 vvm, and at the agitation velocity of 200 rpm in this laboratory scale fermenter.