Streptomyces levendulae의 원형질체와 원형질체 융합에 대한 미세구조

하영칠,홍순우,유진철,임헌만 한국미생물학회 1986 미생물학회지 Vol.24 No.3

Streptomyces lavendulae에서 생성된 원형질체 및 원형질체 융합과정에 대한 미세구조 및 행태학적 관찰을 투과 전자현미경과 주사전자현미경을 사용하여 행하였다. 생성된 원행질체들은 고장액에서 대체로 안정하였으나 간혹 변형된 원형질체들이 관찰되었다. 원형질체 융합은 접촉지역 융합지역, 분리지역 형성과정을 차례로 거치면서 선행되는 것이 관찰되었다. 이러한 사실은 세포막 구조의 변화와 이원형의 소실에 의하여 뒷받침이 될 수 있으으로 이러한 변화들을 융합과정에서의 각 단계로 해석하는 것이 가능하리라 사료된다. Morphology and ultrastructure of protoplast fusion mode in Streptomyces lavendulae were studied by scanning and transmission electron microscopy. The isolated protoplasts were stable in some degree in hypertonic solution except that several protoplasts showed irregular morphology. Fusion events were occurred as follows; contact zone, fusion zone and separation zone were appeared sequentially. After formation of the separation zone, cytoplasm and DNA from both parents were mixed eventually. In the contact zone, two menbranes were still separated by electron transparent space. The contact zone changed to fusion zone by formation of fusion membrane that phospholipid molecules of two membranes were rearranged. Thereafter, nonmembraneous separation zone was formed by disappearance of fusion membrane. These changes were characterized by successive changes in typical membrane structure in fusion areas and by a progressive loss of bispherical shape.

다양한 미생물의 세계

하영칠,Ha, Yeong-Chil 한국과학기술단체총연합회 1993 과학과 기술 Vol.26 No.7

환경오염 유해색소의 미생물학적 분해

하영칠,홍순우,한홍의 한국미생물학회 1983 미생물학회지 Vol.21 No.4

독성이 있으며 난분해성 색소물질의 일종인 푸르푸랄을 분해할 수 있는 4종의 Gram 음성 세균을 P P. testosteroηi, P. maltophila, Klebsiella pneumoniae 그려고 P. fluorescens로 동정하였다. 이 화합물을 분해 할 때, P. testosteroni와 기타 세 균주의 분해 상승효과와 가능한 분해순서를 manometric technique에 의하여 시도되었다. P.testosteroni가 푸르푸랄을 분해하면, 이때 생성된 미확인 물질 NPS를 P. maltophilai와 K.pneumoniae가 이용하면서 생장하게 되고, 동시에 이때 생성된 2-푸로인산을 분해하여 P. fluorescens가 생장하게 된다. 그러나 NPS와 2-푸로인산플 경쟁적으로 분해함으로써 푸르푸랄의 분해효과를 상승시킨다. Isolated Gram-negative bacteria, being capable of degrading toxic, recalcitrant, and pigment-producing furfural, were tentatively identified as Pseudomonas testosteroni, Pseudomonas maltophilia, Klebsiella Pneumoniae, and Pseudomonas fluorescens. They exhibited synergistic effects between P. testosteroni and the others in the degradation of colourproducing furfural. Synergistic effects and possible sequence of its degradation were attempted by manometric technique. P. testosteroni could degrade furfural to decolourize it and produce ninhydrin-reaction postive substance (NPS) which could be utilized by P. maltophilia and K. pneumoniae and the latter two bacteria could ,degrade furfural to 2-furoic acid as an oxidized form. Finally 2-furoic acid was further oxidized by P. fluorescens. Once NPS and 2-furoic acid were produced, the degradation efficiency was enhanced by competing four bacteria against furfural and 2-furoic acid.

한국의 과학기술자-인암 홍순우

하영칠,Ha, Yeong-Chil 한국과학기술단체총연합회 1988 과학과 기술 Vol.21 No.11

Streptomyces tubercidicus에서 glutamine phosphoribosylpyrophosphate amidotransferase의 정제 및 특성

하영칠,유진철 한국미생물학회 1991 미생물학회지 Vol.29 No.2

Glutamine phosphoribosylpyrophosphate amidotransferase of Streptomyces tubercidicus was purified and characterized. Molecular weight of the isolated enzyme was determined to be approximately 230,000 and was composed foru identical subunits having a molecular weight of 58,000. This enzyme was strongly inhibited by AMP while considerably inhibited by ATP and GTP. Inhibition effect of enzyme activity by AMP was antagonized by increased concentration of substrate, PRPP, and metal ion (especially, $Mg^{++}$) was essential in both catalytic activity and nucleotide inhibition of this enzyme. Therefore, it was confirmed that end product inhibition of glutamine phosphoribosylpyrophosphate amidotransferase by adenine participated in the regulation of tubercidin biosynthesis from Streptomyces tubercidicus.s.

진해만의 수질과 생태계에 관한 요인분석에 관하여

하영칠,홍순우,안태석 한국물환경학회 1985 한국물환경학회지 Vol.1 No.1

진해만의 6개 정점의 표·저층에서 1976년 7월부터 1982년 12월까지 물리화학적 생물학적인 환경요소를 65회에 걸쳐 조사하였다. 이들 자료를 통계학적인 분석을 통하여 진해만의 수질 및 생태계에 영향을 미치는 요인을 분석 하였다. 그 결과 행암만 내부에 위치한 정점6을 제외한 전 해역에서 식물성 플랑크톤의 이상증식이 주요한 요인으로 생태계의 44∼56%의 변화를 설명할 수 있었으며, 정점6에서는 영양염류가 중요한 요인이 됨을 알았다. 두번째 요인으로는 담수의 유입으로 볼 수 있었으며. 이 요인은 외해로 나갈수록 그 영향이 적어져, 정점4에서는 의미가 없었다. 질소의 유입도 각 정점에서 주요한 요인이었으며, 그 외에 유기물의 증가 도시하수의 유입, 영향염류의 침전및 미생물에 의한 염류의 재순환과 광합성이 진해만에서의 수질과 생태계를 지배하는 주요 요인으로 확인되었다. In Jinhae bay, the physicochemical, biological and ecological surveys were carried out 65 times at six sites both in surface and bottom water from July 1976 to December 1982. Twenty one abiotic and biotic parameters were determined on each sample. These data were subjected to factor analysis to scrutinize the effects of environmental conditions on water quality and ecosystem. The phytoplankton blooming was the major factor of the variance of the water quality and ecosystem in Jinhae bay. Fresh water inflow was second factor at site 1 and 6, and it was third factor in site 3 and 5. The nitrogen inflow was also play an important role, which was second factor at site 2, 3, 4 and 5, and third factor at site 1. Beside above factors, the increasing of organic materials, waste water inflow, precipitation and recycling of nutrients, upwelling of sediment, nutrient salts and photosynthesis were also determined for the variation of water quality and ecosystem.

유전자의 (遺傳子) 미세구조 (微細構造)

하영칠 韓國生物敎育學會 1970 생물교육 Vol.4 No.-

Aspergillus nidulans FGSC 159 의 β - Glucosidase 특성 및 효소작용

정재성,하영칠,홍순우 ( Jae Sung Jung,Yung Chil Hah,Soon Woo Hong ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.2

Crude enzyme solution prepared from the culture filtrate of Aspergillus nidulans FGSC 159 in CMC minimal liquid medium was fractionated through a three-step procedure including chromatography on Sephadex G-150, DEAE-Sephadex A-50 and Sephadex G-200. Three β-glucosidase components, P-I-Ia, P-II-Ia and P-II-Ib, were prepared. All the fractions had their highest activities at pH 6.0. Optimum temperatures were 55℃ for P-I-Ia, P-II-Ia and 60℃ for P-II-Ib, respectively. P-II-Ia was a little more thermostable than the other two components. The substrates specificities for these β-glucosidase preparations were investigated. These enzymes showed specificities to cellooligosaccharides and sugar derivatives with β-glucosidic linkage such as cellobiose, cellotriose, cellotetraose, cellopentaose, sophorose, PNPG and salicin. All three fractions not only hydrolyzed substrates to remove glucosyl residues one by one from non-reducing end but also transferred the glucosyl residues to other acceptor molecules. But P-I-Ia showed some different mode of action from the other two fractions. It did not accumulate transglucosylated products in detectable amounts, when reacted with cellooligosaccharides.

한강의 수질과 생물지표로서의 세균군 계수

홍순우,하영칠,안태석,이건형 ( Soon Woo Hong,Young Chil Hah,Tae Seok Ahn,Geon Hyoung Lee ) 한국환경생물학회 1983 환경생물 : 환경생물학회지 Vol.1 No.1

Samples were taken from the surface waters of the main stream of Han river at four designated sites in Seoul city area from September, 1980 to August, 1981. Enumeration of total bacteria and pathogenic bacteria grown on selective media and correlation coefficient with six physico-chemical factors were studied. Temperature distribution showed a regular seasonal cycle and ranged from 0.3℃ to 27℃ during investigation. Every sites of water maintained stable pH values ranged from pH 7.5 to pH 7.7. The oxygen content of the water was, relatively high near Cheon Ho Bridge of site I and lowered along the downstream. The monthly values of oxygen concentration varied between 1.6㎎/ℓ and 12.97㎎/ℓ at all sites. It showed that the oxygen depletion was becoming severe along the downstream. Annual average value of BOD were found to be 2.97㎎/ℓ at site 1, 5.70㎎/ℓ at site 2, 7.85㎎/ℓ at site 3 and 8.84㎎/ℓ at site 4. Annual average concentration of ammonium-N varied between 0.17㎎-at N/l and 2.53㎎-at N/l. The nitrite-N concentrations were relatively high comparing with those which were measured in 1977-1978 by Hong et al., their annual values of concentration varied between 10.84㎍-at N/l and 39.04㎍-at N/l. The concentration of nitrate-N were demonstrated similar values of all sampling sites during investigation and they were in the range of 3.21-3.47㎎-at N/l.? The mean annual values of conductivity varied from 0.72mΩ/㎝ to 0. 88mΩ/㎝. The total bacteria counted by NA media were in the range of 5.5×10 exp (3) - 3.6×10 exp (8) cells/㎖. The population density of total bacteria was highest at site 2 and lowest at site 1. Annual average population density of total coliform and fecal coliform by MPN method were in the range of 8.63×10 exp (3) - 2.41×10 exp (5) /100㎖, 1.39×10 exp (4) - 4.21×10 exp (5) /100㎖, respectively. Enumeration of coliforms by Endo and EC media also showed similar population density comparing with MPN methods. The presence of fecal coliforms in surface water was more accurately correlated with fecal discharges than total coliform group. Mean annual population density of salmonella & shigella groups grown on selective media were in the range of 9.68×10 exp (2) - 5.23×10 exp (3) cells/㎖ and that of streptococci and staphylococci were 3-1.75×10 exp (2) cells/㎖, 1.51×10 exp (2) - 2.79×10 exp (2) cells/㎖, respectively. According to correlation coefficients, fecal coliforms at site 2, site 3 and site 4 might closely relate with domestic waste origin of nitrogen sources.

Streptomyces속의 분리 및 동정에 관한 연구

이민재,하영칠,안정선 한국미생물학회 1976 미생물학회지 Vol.14 No.1

A taxonomical sutdy was made on the Streptomyces species isolated from soils in this country, most of which were collected during the period from April, 1974 to July 1975. J.S.P. Methods (1966), I.S.P. Descriptions (1968-1972) and Bergy's Manual of Determinative Bacteriology (1974) were used for most of the experimental methods and identifications. As a result, 24 species were identified as follows ; S. albolongus, S galticri.S. Nashvillensis. S. showdoensis. S.norbonensis. S. flacocirens. S. resistomycificus. S> reshiriensis. S. chromofuscus. S. parvullus. S, chibaensis. S.canus. S. albulus. S. amlachiticus. S.griseoflavus. S. griscoincarnotus. .S. rubiginosus. S. bacillaris. S. setonill. S.intermedius. S. griseinus. S.subrutilus. S.reseosporus.