보문 : Glucose Oxidase로 손상된 배양 인체피부섬유모세포에 대한편백나무 잎 추출물의 항산화 효과

최유선 ( Yu Sun Choi ),박신희 ( Shin Hee Park ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2014 대한미용학회지 Vol.10 No.4

To examine the antioxidative effect of the extract from Chamaecyporis obtusa leaves (COL) on cultured human skinfibroblasts (Detroit 551) damaged by reactive oxygen species (ROS), the cell viability, electron donating ability (EDA),superoxide dismutase (SOD)-like activity and inhibitory activity of lipid peroxidation were measured by colorimetricassay. Cultured Detroit 551 cells were treated with media containing 20~40 mU/mL glucose oxidase (GO) for 4 hours. Study results indicated that GO significantly decreased cell viability as compared to controls, and that the XTT50 valuewas 35.1 mU/mL of GO. Regarding the antioxidative effect of the COL extract, EDA, SOD-like activity, and theinhibitory activity of lipid peroxidation were all demonstrated. These findings suggested that COL extract was effectivein protecting against GO-induced cytotoxicity via its antioxidative activity, and that COL extract may be a putativeresource for the treatment of ROS-mediated diseases such as skin pigmentation irregularities and atopic dermatitis.

수질오염총량관리체제에서의 한강수계관리기금의 개편방향 연구

조용모 ( Yong Mo Cho ),박신희 ( Sun Hee Park ),김현경 서울시정개발연구원 2011 연구보고서 Vol.2011 No.7

Introduction: Since the enforcement of Act Relating to Han River Water Qualtiy Improvement and Community Support in 1999, there has been a significant change in Han River Watershed management environment, One of the most critical change to note is the compulsory application of TMDL (Total Maximum Daily Loading) system in Water Pollution Management, Over the past 10 years, the communities in the upper reaches of the river have been supplied with funding resources that were collected from the lower reaches under the pretext of compensation to land use restrictions in the upper region. Once the TMDL Management system is in effect, however, development of the land within the limit of maximum daily load is allowed, which in turn makes those existing premises of supporting upper reaches no longer valid. Thus, this study aims to suggest the improvement strategies on the Han River Fund management system that accords with the changing conditions, as well as to provide grounds for supplying fund resources for the management of lower reaches of the river. 2. Main Findings: The Current Status of Han River Management Fund -From 1999 to 2008, 3.102 trillion won had been raised for the Fund and 3.074 trillion won had been spent. - Kyunggi province was the biggest beneficiary of the fund (48.5%), followed by Han River Watershed Management Committee Secretariat (22.0%) and Kangwon province (18.4%). -The majority of the fund was spent for installation and operation of water quality improvement facilities(44.1%), followed by the shoring-up project for people in restricted areas(23.1%) and the purchase of land in Riparian Buffer Zones(20.4%). A Rise in Installation and Operation Cost of Water Quality Improvement Facilities, -From 2000 to 2010, the number of water quality improvement facilities in the upper reaches of Han River increased by 3 fold(from 57 to 1,677) and the funding resource spent for the installation increased from 44.6 billion won to 109.3 billion won accordingly. -During the same time period, operating cost increased by 2.5 times from 32.6 billion won to 82.6 billion won. Emerging Fund Management Issues under the New Watershed Management Regime -The upper reaches of the Han River is too vast to be managed via management fund. -There is a call for expanding fund-beneficiary regions to the lower reaches under the Total Water Pollution Load Management Regime. -Installation of water quality improvement facilities can be abused to justify land use development in the upper reaches. In addition, excessive installation and operation of such facilities can lead to fund deficit which creates down spiral. In the meantime, each local ``authorities`` sewer charge is in decline every year. -Nearly 20% of raised fund was spent on the purchase of land in Riparian Buffer Zones, but result was yet insignificant since the purchase of the land was based on negotiation rather that mandatory purchase of the designated area. -All the more, Special Act on Development of Buffer Zone, newly announced in 2010, have directly conflicting goals (of promoting development of riparian zones as the residential/commercial/industrial/cultural/leisure district) for the existing buffer zone conservation policy. - Due to lack of consistent, long-term strategies, community support project has failed to contribute much to improve community life quality. In spite of money invested, therefore, resident satisfaction for the projects turned out to be low. 3. Policy Recommendations: Re-establishing fund raising and distributing principles - Under the new watershed management regime, land regulation of the upper reaches of the river will be eased. Thus communities in the upper reaches should contribute a certain portion to the fund based on ``polluter pays principle``. -Act Relating to Han River Water Quality Improvement and Community Support should be revised to specify this new fund raise and distribution principles. -Installation and operation costs of water quality improvement facilities should be covered by sewer charges of individual local authorities, not by fund resources. Expediting transition into TMDL Management system -Establishing databases and water quality monitoring systems and etc. is a prerequisite for implementing the new management system and thus fund investment to such projects is needed. -Under the new regime, fund-beneficiary regions should be extended to the lower reaches of the river. -Local authorities that avoid implementing total water pollution load management system should be excluded from the fund beneficiaries. Improving existing water quality management system -Purchasing land in Riparian Buffer Zones has proven to have insignificant effect on water quality improvement and the project directly conflicts with the goals of recently enacted Special Act on Development of Buffer Zone. Investing fund resources to the existing system should be avoided and instead, an alternative non-point pollution treatment system should be adopted. -For the impossible task of maintaining water quality standards of 1a throughout the Paldang-lake watershed, an enormous sum of funding resources is being wasted. Thus, designation of intensive water quality management area dubbed as priority drinking water conservation area is needed. -In order to avoid excessive installation and operation (and following fund investment) of water quality improvement facilities, quality management and thorough speculation of facilities` treatment capacity and water quality improvement performances is needed. -Due to the increase in non biodegradable pollutants in the watershed in recent years, distributing fund resources to the installation of tertiary treatment facilities is more urgently required than to the capacity expansion of existing facilities. Adoption of Integrated Watershed Management System -Shifting from the Ministry of Environment`s centralized fund management and distribution system, a new governance structure which enables local governments` active participation in the fund execution process should be created. -More specifically, hands-on workers from the local authorities should be dispatched to the Watershed Management Committee Secretariat and a chairman of the Committee should be delegated to the governors of the 5 local authorities.

수질오염총량관리체제에서의 한강수계관리기금의 개편방향 연구

조용모(Yong-Mo Cho),박신희(Sun-Hee Park),김현경 서울연구원 2011 서울연구원 정책과제연구보고서 Vol.- No.-

경피적 내시경적 괴사 제거술에서 완전 피복 식도 금속 스텐트의 유용성

이나겸 ( Nah Kyum Lee ),박도현 ( Do Hyun Park ),박신희 ( Shin Hee Park ),박수연 ( Su Yeon Park ),정수민 ( Soo Min Jeung ),오진선 ( Jin Sun Oh ),서보미 ( Bo Mi Seo ) 대한췌담도학회 2015 대한췌담도학회지 Vol.20 No.2

괴사성 췌장염은 사망률이 높고 괴사된 췌장 부위에 감염이 동반되어 있을 경우에 패혈증과 다발성 장기 부전이 발생하여 사망률이 더욱 증가하는 중대한 질환으로 과거에는 수술적 괴사 제거술이 많이 시행되었지만 최근에는 경피적 배액술 또는 내시경적 괴사 제거술과 같은 최소 침습 시술이 치료에 널리 이용되고 있다. 저자들은 경피적 배액술과 내시경적 배액술을 시행하였으나 치료 반응이 없고 임상적으로 악화 경과를 보이는 환자에서 직경이 넓은 식도 금속 스텐트를 사용하여 경피적 내시경적 괴사 제거술을 시행하여 대장주변(paracolic gutter)에 있는 막으로 둘러싸인 괴사를 성공적으로 치료한 사례들을 경험하였고 식도 금속 스텐트를 사용하여 경피적 내시경적 괴사 제거술이 시행된 증례는 국내에서 이전에 보고된 바가 없기에 이를 문헌 고찰과 함께 보고하는 바이다. Necrotizing pancreatitis is associated with high rates of morbidity and mortality. Managing necrotizing pancreatitis is challenging, and minimally invasive treatment modalities recently replaced traditional open necrosectomy. Percutaneous catheter drainage and endoscopic necrosectomy are now widely used because they are less invasive, safer, and can more effectively remove necrotic materials. Various methods and novel techniques have been introduced to manage walled-off necrosis. Herein, we report a case series of patients with necrotizing pancreatitis who were successfully treated using fully covered esophageal metal stent and endoscopic necrosectomy via the percutaneous approach. Percutaneous endoscopic necrosectomy using a fully covered esophageal stent is an effective endoscopic treatment for patients with walled-off necrosis that extends along both sides of the paracolic gutter, reduces the number of necrosectomy sessions, and improves disease status faster than conventional treatment. Korean J Pancreatobiliary 2015;20(2):88-93

보문 : 모발염색제 성분인 초산납에 대한 어성초 추출물의 항산화 및 미백효과

정재윤 ( Jai Yun Jung ),오영희 ( Young Hee Oh ),박신희 ( Shin Hee Park ),윤미영 ( Mi Young Yoon ),표애자 ( Ae Ja Pyo ),김선주 ( Sun Ju Kim ),유영월 ( Yeong Wol Yu ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2014 대한미용학회지 Vol.10 No.2

To evaluate the antioxidative and whitening effects of Houttuynia cordata Thunb (HCT) extract on lead acetate (LA), hair dye component, cell viability was measured by colorimetric assay after NIH3T3 fibroblasts were cultured in media containing various concentrations of LA. And also, the effect of antioxidant, vitamin E on LA-induced cytotoxicity was analysed. For the protective effect of HCT extract on LA-induced cytotoxicity, NIH3T3 fibroblasts were pretreated with 110 or 130 μg/mL of HCT extract for 2 h before the treatment of LA. And also, the antioxidative and melanogenic effects of HCT extract were assessed by superoxide dismutase (SOD)-like activity, total amount of melanin and tyrosinase activity. In this study, LA significantly decreased cell viability in dose-dependent manner. And the XTT50 value was calculated at 48.3 μM of LA. In the effect of antioxidant, vitamin E, it effectively prevented LA-induced cytotoxicity by the significant increase of cell viability. In the protective effect of HCT extract on LA-induced cytotoxicity, HCT extract significantly increased cell viability which was decreased by the cytotoxicity induced by LA, and also it showed the antioxidative effect by SOD-like activity. In the melanogenesis, HCT extract showed the decreased of amount of melanin and tyrosinase activity. From these results, it is suggested that the cytotoxicity of LA is involved in oxidative stress, and HCT extract effectively prevented the cytotoxicity and the melanogenesis induced by LA.

근적외선분광광도법을 이용한 식용유지의 산가, 요오드가, 지방산 정량법에 관한 연구

김재관(Jae-Kwan Kim),최옥경(Ok-Kyung Choi),황선일(Sun-Il Hwang),정진아(Jin-A Jeong),김윤성(Yun-Sung Kim),박신희(Sin-Hee Park),손미희(Mi-Hui Son),권혜정(Hye-Jung Kwon),이정복(Jung-Bock Lee),김종찬(Jong-Chan Kim) 대한환경위생공학회 2007 대한환경위생공학회지 Vol.22 No.4

The possibility of rapid quantitative analysis of AV(Acid Value), Ⅳ(Iodine Value) and fatty acids in vegetable oils with NIRS(Near-infrared spectroscopy) was evaluated. A calibration equation calculated by MPLS regression technique was developed and correlation coefficient of determination for AV, Ⅳ, C16:0, C16:0, C16:1, C16:2, C16:6, and C20:0 content were 0.9727, 0.997, 0.9805, 0.942, 0.9987, 0.9994, 0.9966, and 0.975 respectively. According to the data obtained from validation study, R² of contents of perilla, corn, soybean, rapaseed oils were 0.897, 0.993, 0.935, 0.707, 0.994, 0.996, 0.984, 0.798, SEP of contents of 0.185, 1.367, 0.899, 0.640, 1.498, 1.360, 0.476, 0.076 by MPLS. The results indicate that the NIRS procedure can potentially be used as a non-destructive analysis method for the rapid and simple measurement of AV, Ⅳ and fatty acids in vegetable oils.

유방암 과거력이 있는 환자에서 발생한 복막가성점액종

정태섭 ( Tae Seob Jung ),홍정운 ( Jung Un Hong ),박신희 ( Shin Hee Park ),이희정 ( Hee Jeong Lee ),민인선 ( In Sun Min ),이주영 ( Ju Young Lee ),석창현 ( Chang Hyeon Seock ) 대한소화기학회 2016 대한소화기학회지 Vol.67 No.3

Pseudomyxoma peritonei is a very rare condition, and even rarer in patients with history of cancer. A 70-year old woman with a history of breast cancer was admitted with abdominal pain and distention. Abdominal computed tomography revealed ascites collection, diffuse engorgement and infiltration of the mesenteric vessel, suggesting peritonitis or peritoneal carcinomatosis. Diagnostic paracentesis was attempted several times, but a sufficient specimen could not be collected due to the thick and gelatinous nature of the ascites. Therefore, the patient underwent diagnostic laparoscopy for tissue biopsy of the peritoneum, which indicated pseudomyxoma peritonei. However, the origin of the pseudomyxoma peritonei could not be identified intraoperatively due to adhesions and large amount of mucoceles. Systemic chemotherapy was performed using Fluorouracil, producing some symptomatic relief. After discharge, abdominal pain and distention gradually worsened, so at 18 months after initial diagnosis the patient received palliative surgery based on massive mucinous ascites and palpable mass at the omentum. The patient expired after surgery due to massive bleeding. (Korean J Gastroenterol 2016;67:153-157)