호텔 브랜드와 객실판매간 상관성 연구

박승택(Seung-taek Park) 한국서비스산업학회 2007 서비스산업연구 Vol.4 No.2

산소자유기에 의해 손상된 배양 희소돌기아교세포에 대한 Iron-chelator의 영향

박승택(Seung Taeck Park),문연자(Yeun Ja Mun),오재민(Jae Min Oh),김정중(Jung Joong Kim),최민규(Min Kyu Choi),심재한(Jae Han Shim),임계택(Kye Taek Lim),정연태(Yeun Tai Chung) 대한체질인류학회 1996 해부·생물인류학 (Anat Biol Anthropol) Vol.9 No.2

간추림 산소자유기의 신경옥성에 대한 기전을 규명하기 위하여 소의 애양 희소돌기아교세포를 산소자유기에 노출시킨 뒤 이의 독성효과를 조사하고 또한 산소자유기에 의하여 유도된 신경독성에 대한 Iron-chelator의 방어효과를 MTT 분석법에 의하여 알아보았다. 배양 희소돌기아교세포를 20mU/ml XO 와 0.lmM HX가 혼합된 배양액에서 1~8 시간 동안 배양한 결과 산소자유기는 시간에 비례하여 세포의 생존율을 현저하게 감소시켰다. 또한 산소자유기에 의하여 유도 원 신경독성에 대하여 iron-chelator인 tetrakis (2-pyridylmethyl)ethylenediamine (TPEN)은 유의하게 방어효과를 나타낸데 비하여 또 하나의 Iron-chelator 인 desferroxamine (DFX)은 유의한 방어효과를 나타내지 않았다. 이상의 결과로 부터 산소자유기는 소의 배양 희소돌기아교세포에 독성을 나타냈으며 TPEN 과 같은 선택적인 iron chelator 가 신경옥성을 방어한테 효과적인 것으로 나다났다.

매실추출물의 항산화력 탐색

박명우,김미라,임계택,박승택,심재한,Park, Myung-Woo,Kim, Mi-Ra,Lim, Kye-Taek,Park, Seung-Taeck,Shim, Jae-Han 한국응용생명화학회 2002 한국농화학회지 Vol.45 No.2

매실(Prunus mune Sieb. et Zucc.)의 품종간, 그리고 수확기에 따라 매실추출물의 항산화력을 알아보기 위하여 본 실험을 수행하였다. 먼저 항산화력은 1,1-diphenyl-2-picryl hydrazyl(DPPH), thiocyanate, glutathione-Stransferase와 같은 화학적 방법과 생물학적 방법으로 mouse의 spinal cord culture system에 superoxide anion을 발생시켜 측정했다. 일반적으로 매실 에탄을 추출물은 항산화력을 갖고 있었으며 DPPH, thiocyanate방법에 있어서 butylated hydroxy toluene(BHT)와 비교하였을 때 옥영이 다른 품종보다 97.5%로 제일 높았으나 품종간에 유의성은 없었다. Glutathione-S-transferas방법으로 butylated hydroxy toluene(BHT)와 매실추출물의 항산화력을 비교해본결과 70% 이상의 강한 항산화력을 보여주었다. 생쥐의 척수신경세포 배양시 세포생존율은 옥영, 남고, 고전매추출물을 40 mg처리했을 때 각각 89, 79그리고 62%였고, 6린M의 glutathion을 처리했을때는 90%였다. Antioxidative activities of ethanol extracts of fruits from three cultivars of Prunus mune with different harvest times were determined. Extracts of P. mune cutivar Okyoung showed the strongest activity at 97.5%(compared to BHT) using DPPH or thiocyanate method, although no statistically significant differences were observed among the cultivars. Antioxidative activities of the extracts measured with glutathione-S-transferase method was over 70% of that of BHT. Survival rates of cells in mouse spinal cord culture challenged by superoxide anion were 89, 79, and 62% at 40 mg/ml of Okyoung, Namgo, and Kochunme extracts, respectively, whereas 90% at 6mM glutathion.

Estradiol과 Medroxyprogesterone Acetate가 골아세포의 성장에 미치는 영향

김기석 ( Kie Suk Kim ),민부기 ( Bu Kie Min ),홍기연 ( Gi Youn Hong ),박승택 ( Seung Taek Park ),이승필 ( Seung Phil Lee ),김인숙 ( In Suk Kim ) 대한폐경학회 2001 대한폐경학회지 Vol.7 No.1

N/A This study was designed to clarify the hormonal effect on the growth of osteoblast. And the osteoblasts from neonatal mouse were cultured in the medium at various concentrations of 17β-estradiol(EST), medroxyprogesterone acetate(MPA) in addition 10nM of dexamethasone(DMS) and alcohol for 48 hours. And in order to detect the response of osteoblast on the those hormones, the alkaline phosphatase(ALP) activity also was measured. 1. At 48 hours after culture of osteoblasts in media at various concentrations of hormones, EST has an effect on increasing of the cell number in fashion of dose dependent, whereas MPA did not increase the cell number. 2. Dexametasone significantly promoted differentiation of osteoblasts does dependently with increasing of ALP activity. 3. In osteoblasts culture in media supplemented with alcohol in the absence of 10nM DMS, EST increased significantly ALP activity, while decreased ALP activity of osteoblast in the presence of DMS, 4. In culture of osteoblasts treated with alcohol in the absence of 10nM DMS, MPA did not show any change of ALP activity. But in the presence of DMS, MPA decreased ALP activity of osteoblast. 5. EST plus MPA stimulated the ALP activity in osteoblasts treated with alcohol in the absence of DMS. While, in the cells treated with DMS, they synergistically decreased ALP activity.

흰쥐 평활근세포에서 gliotoxin 세포독성에 대한 PKC의 효과

방현주(Hyun-Ju Bang),김진오(Jin-O Kim),오정택(Jung-Taek Oh),이한솔(Han-Sol Lee),정용관(Yong-Kwan Cheong),허정무(Jung-Mu Hur),오재민(Jay-Min Oh),최민규(Min-Kyu Choi),박승택(Seung-Taeck Park),정연태(Yeun-Tai Chung) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.5

Gliotoxin은 Penicillium, Aspergillus, Gliocladium 및 Thermoascus와 같은 곰팡이들로부터 생성되며 여러 가지 곰팡이에 의한 질병이나 면역조절기능 같은 다양한 생리적 효과를 나타낸다. 최근에 다양한 세포주에서 gliotoxin에 의한 아폽토시스의 기전이 밝혀지고 있으며, Gliotoxin에 의한 아폽토시스 유도 시 칼슘의 농도가 증가하는 현상이 보고 되었다. 본 연구에서는 칼슘 및 칼슘과 상보적인 PKC를 전처리 한 세포에서 gliotoxin의 효과에 대하여 살펴보았다. Gliotoxin에 의한 세포의 손상에는 bcl-2 family인 bad의 발현이 증가하는 현상을 보였으며, 그와 반대로 bcl-2의 발현은 감소하였다. 또한 gliotoxin을 처리한 세포에서는 시간 의존적으로 caspase-3의 활성 증가와 DNA 분절현상이 관찰되었다. 칼슘과 PKC를 전 처리한 세포에서는 gliotoxin에 의한 세포의 손상을 억제하였으며, bcl-2의 발현이 대조군과 유사하였고 bad는 발현되지 않았다. 또한 caspase-3를 비활성화 시키고, DNA 분절을 억제시켰다. 이들 결과를 통하여 알 수 있는 것은, gliotoxin에 의한 세포의 죽음은 사립체에서 cytochrome c의 방출과 동시에 pro-apoptotic bcl-2 family의 발현을 통해서 caspase-3가 활성화되고, 그 결과 DNA 분절을 일으켜 아폽토시스를 유발시키는 것으로 추정된다. 칼슘 및 PKC를 전처리한 경우에는 사립체에서 bcl-2 family 부분에서 bad와 같은 pro-apoptotic bcl-2 family의 발현을 억제시킴으로써 그 후에 오는 caspase-3의 활성을 차단하는 것으로 사료된다. Aspergillus funigatus and other pathogenic fungi synthesize a toxic epidithi- odiopiperzine (ETP) metabolite, namely gliotoxin. Gliotoxin commonly react with sulfhydryl groups, and then, forms hydrogen peroxide. These fungal toxins induce apoptotic cell death in various cells. Apoptosis induced by gliotoxin need calcium. Effect of calcium preconditioning was not reported in gliotoxin-induced apoptosis. To examine the effect of protein kinase C (PKC) and calcium which was regulate caspase-3, PKC and calcium preconditioning before gliotoxin treatment, apoptotic agents such as bcl-2 family, caspase-3 and DNA fragmentation in A7r5 cell line from rat smooth muscle cell were studied. These results showed that gliotoxin induces the expression of bad of bcl-2 family, caspase-3 activation and DNA fragmentation in A7r5 cells. Gliotoxin treatment followed by calcium and PKC preconditioning suppress the Bad of bcl-2 family, and inhibited caspase-3 activation, respectively. These results suggest that PKC and calcium preconditioning protect the gliotoxin-induced apoptosis, through the protection of pro-apoptotic bcl-2 family in A7r5 cells.

際草劑 마세트가 성게의 發生段階에 미치는 影響에 관한 形態學的 硏究

朴承澤 圓光大學校 基礎自然科學硏究所 1984 基礎科學硏究誌 Vol.3 No.1

The effects of the herbicide Machete on the developmental stages of sea urchin, Hemicentrotus pulcherrimus embryos were studied. The collected embryos were induced to develop from the fertilized eggs to gastrula in sea water with varying concentrations of the Machete. Toxicities of the Machete on the development of the embryos were examined by comparing the rate of development in each stage between the control and the testing groups. Machete suppressed the development of embryos and the inhibitory effect on the 2-cell stage was more pronounced. The followings show estimated threshold concentrations of each stages: Fertilization membrane formation, 0.2ppm: 2-cell stage, 0.02ppm: Blastula, 0.05ppm: Gastrula, 0.1ppm.

2價水銀이 생쥐胚芽의 初期發生段階에 미치는 影響

朴承澤,鄭然泰 圓光大學校 醫科學硏究所 1986 圓光醫科學 Vol.2 No.1

In order to evaluate the cytotoxity of mercury ion(Hg^2+), morphological and biochemical observation was performed to examine the threshold concentration of inhibition and protein synthesis on the development of early embryos which were cultured from two cells to blastocysts in the medium containing various concentrations of Hg^2+. The results were summerized as follows : 1. The development of two-cell embryos were suppressed remarkably at 2.5ppm of Hg^2+. 2. Two-cell stage was more sensitive than early morula to Hg^2+ in each developmental stages of early embryos. 3. Mercury ion(Hg^2+) inhibited both the uptake and incorporation of ^3H-leucine in the two-cell embryos exposed to Hg^2+ for 6 and 24 hours respectively. This result suggest that mercury ion(Hg^2+) suppressed the development of early embryos by inhibiting the protein synthesis.

Methylmercury 가 생쥐 대뇌 신경세포에 미치는 영향

박승택,김종영,문연자,임계택,최민규,정연태 원광대학교 환경과학연구소 1995 環境科學硏究誌 Vol.4 No.1

재발성 가와사끼병의 임상양상과 검사소견

유승택,최은정,이창우,오연균,박승택,윤향석 圓光大學校 醫科學硏究所 2003 圓光醫科學 Vol.18 No.2

Acetaldehyde가 사람 染色體에 미치는 細胞遺傳學的 影響에 關한 연究

朴鍾鶴,朴承澤,韓斗錫,鄭然泰 圓光大學校 醫科學硏究所 1986 圓光醫科學 Vol.2 No.1

To investigate the effect of acetaldehyde on the human chromosomes especially frequency and distribution of sister chromatid exchange, an attempt was made. The results obtained were as follows : The frequencies were found to be 17.98±0.49, 12.97±0.34, 10.98±0.26 and 7.24±0.24 in treated with acetaldehyde 10^-3, 5×10 exp (-4), 10^-4 M and the control respectively. Acetaldehyde produced the dose dependent increase of frequency of SCK. The regions generally vulnerable to acetaldehyde were 1q23 in chromosome 1, 2q31 in chromosome 2 and 3q21 in chromosome 3. Among these regions, 2q31 in chromosome 2 was particularly susceptible to acetaldehyde Most of these regions susceptible to acetaldehyde were corresponding to G-light bands.