Comparative proteomic analysis of host responses to Plasmodiophora brassicae infection in susceptible and resistant Brassica oleracea

문주연,김선태,최경자,권석윤,조혜선,김현순,문재선,박정미 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.3

Clubroot disease, caused by Plasmodiophora brassicae, is one of the most devastating diseases affecting members of the Brassicaceae family. It is difficult to control by chemical or cultural means, and the molecular mechanisms underlying interactions with Brassica oleracea (cabbage) remain poorly understood. Herein, we used a proteomic approach to investigate B. oleracea–P. brassicae interactions during the early phases of infection in above-ground tissues. Proteins were isolated from the aerial parts of clubroot-susceptible (CT-18) and -resistant (YCR) cabbage cultivars at 5 days after inoculation with P. brassicae or buffer (mock) and resolved by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis. A total of 24 differentially modulated proteins were identified in at least two biological replicates, and exhibited altered expression between mock and P. brassicae treatments and/or in the different cabbage cultivars. Most of the identified proteins are involved in oxidative stress, abscisic acid (ABA) metabolism, glucose-mediated signalling and responses to stimuli. Resistant YCR plants harboured an increased abundance of ABA-responsive protein, fructose-bisphosphate aldolase and glucose sensor interaction protein compared with CT-18 plants in both mock and P. brassicae-treated samples, suggesting that they may mediate basal defences against P. brassicae infection in YCR. Specifically, we observed that susceptible (CT-18) plants expressed higher levels of cobalamin-independent methionine synthase than YCR, which may enhance susceptibility of the host. Further investigation of the identified proteins will likely facilitate the identification of key molecular determinants, potentially improving clubroot disease resistance in future cabbage crop species.

한국판 청소년용 스트레스 대처 유연성 척도(SFCS-KA)의 타당화

문주연,이은주 한국교육심리학회 2024 敎育心理硏究 Vol.38 No.1

This study was conducted to validate the Self-perception of Flexible Coping with Stress Scale for Korean Adolescents(SFCS-KA) by applying the Rasch measurement model considering content, substantive, structural, generalizability, and external aspects of validity(Messick, 1995). Data of 1249 Korean elementary, middle, and high school students were analyzed. The results were as follows: First, the item fit statistics for each item were satisfactory. Second, the 5-point scale functioned more appropriately than the 7-point scale for all three factors(i.e., multiple coping strategy use, situational coping, and coping flexibility). Third, principal component analysis of standardized residuals demonstrated the unidimensionality of each of the three SFCS-KA subscales. Additionally, CFA showed that a three-factor model was the most favorable. Fourth, person and item reliability and person and item separation indices were of a satisfactory level. Also, the three SFCS-KA subscales did not function differently for elementary, middle, and high school levels or for male and female students. Fifth, multiple coping strategy use and situational coping were positively and negatively associated with the positive and negative criterion variables, respectively. However, for coping rigidity correlational patterns were the opposite. Sixth, Cronbach’s α for the items loading on three factors was satisfactory. Considering these findings, the SFCS-KA proved to be a reliable and valid measure of the self-perceived ability to cope flexibly with stress in Korean adolescents. Educational Impact and Implications : The SFCS-KA can be used to identify students who are at greater risk of employing flexible coping strategies for academic stress. Thus, it will be based on future interventions aimed at promoting students’ coping flexibility in academically stressful situations. Furthermore, the SFCS-KA will provide a brief means of diagnosing students’ strengths and weaknesses in multiple coping strategy use, situational coping, and coping rigidity. Thereby, it will contribute to the development of intervention programs to capitalize on strengths and compensate for weaknesses in students’ ability to cope flexibly with academic stress.

보강재 수리시 리벳 배열이 응력 변화에 미치는 영향 연구

문주연,신기수,Moon, Ju-Yeon,Shin, Ki-Su 한국국방경영분석학회 2010 한국국방경영분석학회지 Vol.36 No.1

보강재 수리는 항공무기체계 구조물에서 발생하는 각종 균열결함을 수리하기 위한 수리기법 중의 하나이다. 최근 들어 일부 장기운영 항공기에 대한 균열수리 소요는 증가추세에 있으나, 수리기법에 대한 군내에서의 연구는 미비한 실정이다. 본 연구는 보강재 수리방법에 따른 균열수리효과 검증을 목적으로 수행되었다. 특히 수리효과에 직접적으로 영향을 미치는 리벳 배열과 응력집중과의 상관관계를 고찰하였다. 상용 유한요소해석 프로그램을 사용하여 수리방법에 따른 응력의 변화량을 정량적으로 산출하였다. 연구결과 수리강도에 가장 크게 영향을 미치는 요소는 피치와 줄 수임을 확인하였다. 또한 본 연구를 통해 보강재 크기별 최적으로 리벳 배열 방법을 제시할 수 있었다. Doubler repair is one of the frequently applied methods to repair cracks which occur at the structure of aircraft weapon systems. The requirement of the doubler repair for some of aging aircraft has increased more recently. However very few researches have been conducted until now. In this paper, the effect of the various doubler repair methods was evaluated. Especially, the relationship between the array of revet and the effect of stress concentration was investigated. To calculate variation of the stress with different repair methods, commercial FEA program was utilized. As a result, it was shown that the main factor for the variation of stress is pitch and number of revet line. Furthermore the optimized array of revet for the specific geometries of doubler was suggested.

유역단위에서의 임목평균흉고직경과 기온 간의 관계: 안동댐 유역을 중심으로

문주연,김문일,임윤진,PIAODONGFAN,임철희,김세진,송철호,이우균 한국농림기상학회 2016 한국농림기상학회지 Vol.18 No.4

This study aims to identify the relationship between climatic factors and stand mean Diameter at Breast Height (DBH) for two major tree species; Pinus densiflora and Quercus mongolica in Andong-dam basin. Forest variables such as age, diameter distribution and number of trees per hectare from the 5th and 6th National Forest Inventory data were used to develop a DBH estimation model. Climate data were collected from six meteorological observatory station and twelve Automatic Weather System provided by Korea Meteorological Administration to produce interpolated daily average temperature map with Inverse Distance Weighting (IDW) method. Andong-dam basin reflects rugged mountainous terrain, so temperature were adjusted by lapse rate based correction. As a result, predictions of model were consistent with the previous studies; that the rising temperature is negatively related to the growth of Pinus densiflora whereas opposing trend is observed for Quercus mongolica. 본 연구는 안동댐 유역의 주요 수종인 소나무와 신갈나무를 대상으로 임분평균흉고직경과 기상인자의 관계를 규명하고자 수행되었다. 본 연구는 제5차 국가산림자원조사(NFI5)자료 중 임령, 임분평균흉고직경, ha당 본수자료를 사용해 임분단위의 평균흉고직경을 추정하는 모형을 개발하고, NFI6 자료로 검증을 실시하였다. 또한 안동댐 유역 수문기상관측소 6개소와 주변지역의 Automatic Weather System (AWS)의 관측자료에서 취득한 기온 자료를 사용하여 100m 공간해상도를 갖는 상세한 기상자료를 구축하였다. 연구대상지가 복잡한 산악지형임을 고려하여 기온감률을 적용 후 Inverse Distance Weighted (IDW) 보간법을 적용하여 점 형태의 기상자료를 면 형태로 재구축하였다. 이와 같이 구축된 정밀기상인자와 직경생장간의 관계를 분석하였다. 분석결과 기온 상승은 소나무에 부(-)의 영향을 주며, 참나무류인 신갈나무에는 정(+)의 영향을 주는 것으로 나타나 기존 연구들과 일치하는 결과를 보였다. 본 연구는 수문기상관측소와 AWS 자료를 연계하여 세밀한 기후지도를 구축하고, 이를 통해 유역규모의 임목생장과 기후인자와의 관계를 파악했다는 것에 의미가 있으며 지역규모의 산림변화 예측 및 관리 계획 수립을 지원할 수 있을 것으로 사료된다.

Role of hydrogen sulfide in cerebrovascular alteration during aging

문주연,Hye-Min Kang,Junyang Jung,Chan Park 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.5

Hydrogen sulfide (H2S), a gasotransmitter molecule, has attracted attention as an endogenous signaling molecule because of its various pathological and physiological effects, including pathologies related to aging. In this review, we aimed to discuss the morphological changes in each vessel following aging, and to evaluate the possible role of H2S in the cerebrovascular alteration, such as arterial stiffness, microvessel loss, and blood–brain barrier disruption, with advancing age. Additionally, the review outlines the therapeutic efficacy of the H2S-releasing compounds on the cerebrovasculature. In the central nervous system, cerebrovascular aging can lead to the loss of blood–brain integrity, which causes cognitive impairment. The findings discussed in this review strongly support the notion that H2S has a potential therapeutic role in cerebrovascular alteration in aging.

청소년용 다차원적 학업스트레스 대처전략 척도의 타당화 연구

문주연,이은주 한국교육심리학회 2022 敎育心理硏究 Vol.36 No.1

This study was conducted to validate the Multidimensional Measure of Academic Coping for Korean Adolescents(MMAC-KA) by applying the Rasch measurement model within the framework of the content, substantive, structural, generalizability, and external aspects of validity as described by Messick(1995). The data of 409 Korean middle school students were analyzed. The results were as follows: First, the item fit statistics for each item were deemed satisfactory. Second, the 4-point scale functioned appropriately for all 11 factors. Third, principal component analysis of standardized residuals demonstrated the unidimensionality of all 11 scales. Fourth, person and item separation indices for 11 factors were found to be of a satisfactory level. Two out of 55 items displayed differential item functioning for gender. Fifth, adaptive coping strategies were positively associated with positive motivational constructs(identified/intrinsic regulation and engagement), whereas maladaptive coping strategies were positively associated with negative motivational constructs (amotivation, external regulation, and disengagement). Based on these findings, the MMAC-KA proved to be a valid and reliable measure of academic coping strategies of Korean adolescents. 본 연구의 목적은 중학교에 재학 중인 청소년 409명을 대상으로 Skinner, Pitzer와 Steel(2013)이 개발한 다차원적 학업스트레스 대처전략 척도를 한국판 청소년용으로 타당화하는데 있었다. 이를 위해 Messick(1995)이 분류한 타당도의 틀 속에서 Rasch 측정모형을 적용해 타당도를 검증하였다. 첫째, 문항 적합도 분석결과 11개 하위척도(전략화, 도움추구, 위로추구, 자기격려, 전념, 혼란, 도피, 은폐, 자기연민, 반추, 투사)의 55개 문항 모두 양호한 적합도 지수 값을 나타내 검사내용에 기초한 타당도가 검증되었다. 둘째, 평정척도 분석결과 4점 척도가 적절하여 실제에 기초한 타당도가 검증되었다. 셋째, 표준화 잔차 분석결과 11개 하위척도 모두 단일차원성 가정을 충족하여 내적구조에 기초한 타당도가 검증되었다. 넷째, 차별기능문항 분석 및 신뢰도 분석을 실시한 결과 2개 문항을 제외하고는 성별에 기초한 차별기능문항은 없는 것으로 나타났으며, 신뢰도 계수 역시 양호한 값을 보여주어 일반화에 기초한 타당도가 검증되었다. 다섯째, 적응적 학업스트레스 대처전략은 긍정적 준거변인(내적 조절, 확인된 조절, 수업참여 등)과는 유의한 정적 상관을, 부정적 준거변인(무동기, 외적 조절, 수업이탈 등)과는 유의한 부적 상관을 보였으며, 부적응적 학업스트레스 대처전략은 부정적 준거변인과는 유의한 정적 상관을, 긍정적 준거변인과는 유의한 부적 상관을 보여 외적 준거에 기초한 타당도가 검증되었다. 본 연구결과를 바탕으로 다차원적 학업스트레스 대처전략 척도가 청소년들이 학업스트레스에 어떻게 대처하는지 알아보고 적절한 개입전략을 마련하는데 유용한 검사도구로 활용될 수 있을 것으로 기대된다.

국내 산림정책과 지속가능발전목표(SDGs)간의 연관성 분석

문주연,김나희,송철호,이슬기,김문일,임철희,차성은,김강선,이우균,손요환,양수길,진새봄,손영모 한국기후변화학회 2017 한국기후변화학회지 Vol.8 No.4

This study analysed the linkage between national forest policy in Korea, namely the 5th National Forest Master Plan, 2016 Korea Forest Service Performance Management Plan, the 3rd National Sustainable Development Plan, and UN Sustainable Development Goals (SDGs). The 7 strategies of the 5th National Forest Master Plan were related to 11 Goals of SDGs, and 5 strategies of 2016 Korea Forest Service Performance Management Plan were associated with 7 areas of SDGs, and 4 strategies within 3rd National Sustainable Development Plan were linked to 7 Goals of SDGs. Among 87 national forest indicators compiled from three respective forest-related policies of Korea, 45 national indicators were related to 18 SDGs indicators. This indicates that 52% of national indicators of Korean forest policy are reflecting the language of SDGs. However, seeing from SDGs perspective, only 18 out of 241, which accounts for 7.8% of SDGs indicators are related to national indicators. The findings imply that a number of national forest-related indicators do not meet the diverse dimension of SDGs which provides potential areas for forest to contribute. Based on the findings, following recommendations were suggested: 1) the term used in forest policy should be aligned to SDGs targets so that it can be embedded in national policies, and 2) indicators should be further contextualized as well as in its assessment system. Lastly, it suggests for leveraging 3) ‘5 Processes of sub-national climate change adaptation plan’ and the core concept of REDD+ MRV which could provide fundamental background for implementing SDGs framework to national forest policy.

생체고분자 단백질 및 RNA의 세포간 이동 조절

문주연,정진희,임영길,김재연,Moon, Ju-Yeon,Jung, Jin-Hee,Rim, Yeong-Gil,Datla, Raju,Joliot, Alain,Jackson, David,Kim, Jae-Yean 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.2

Intercellular signaling is a crucial biological process for the coordination of cell differentiation, organ development and whole plant physiology. The intercellular movement of macromolecule signals such as proteins and RNAs has emerged as a novel mechanism of cell-to-cell communication in plant. Plasmodesmata, which are intercellular symplasmic channels, provide a key pathway for cell-to-cell trafficking of regulatory proteins / RNAs. This review specifically focuses on integrating the recent understanding on non-cell autonomous macromolecules, their function and regulatory mechanisms of intercellular trafficking through plasmodesmata.

Fabrication of Thermosensitive Microparticles for Controlled Drug Delivery

문주연,노윤호,김현웅,봉기완 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0

Controlled drug delivery is important for therapeutic efficacy. Among stimuli-responsive polymers, thermo-responsive polymers have drawn much attention because this stimulus occur in the body and can be given from outside, which makes it easier to control. Here, we introduce Poly(N-vinyl caprolactam)(PVCL) microparticles for controlled drug delivery. Unlike PNIPAm, PVCL has good biocompatibility. Microparticles were synthesized via Stop Flow Lithography technique. We verified that polymer network in microparticles are contracted above certain temperature known as lower critical solution temperature (LCST). After adjusting the LCST to body temperature by adding poly ethylene glycol 700 diacrylate as a hydrophilic component, we conducted drug release using Doxorubicin and revealed that the releasing rates were significantly higher at above LCST than below LCST. Moreover, we fabricated gold nanorod(GNR) incorporated PVCL microparticles to control drug release by exposure of near-infrared.

탈중심화와 경험 회피가 우울에 미치는 영향: 종단적 매개효과

문주연,이민규 한국임상심리학회 2017 Korean Journal of Clinical Psychology Vol.36 No.2

본 연구의 목적은 ACT와 MBCT에서 핵심적 변화기제로 상정한 탈중심화와 경험 회피간의 종단적 관계를 검증한 후, 우울에 탈중심화와경험 회피가 미치는 종단적 매개효과를 검증하는 것이다. 연구대상은 부산, 경남지역 일반인과 대학생 195명(남학생 67명, 여학생 125명, 무응답 3명)이었으며, 한국인우울증척도(KDS), 수용-행동 질문지(AAQ-Ⅱ), 탈중심화(EQ) 척도를 사용하여 8주 간격으로 3차례에 걸쳐실시하였다. 분석에는 가설을 검증하기 위해 종단자료를 이용하여 변수들의 인과관계를 결정하는데 유용한 자기회귀 교차지연 모형(autoregressive cross-lagged model)을 이용하였다. 그 결과 탈중심화와 경험 회피는 서로 상호순환적인 관계를 나타내고 있었다. 또한 1차 시기의 탈중심화가 3차 시기의 우울에 직접적인 영향을 미치지는 않았지만, 경험 회피는 탈중심화와 우울의 관계에서 유의한 종단적 매개효과를 보였다. 이 연구의 의의는 ACT나 MBCT에서 중요하게 여기는 치료요인 간의 인과관계를 명확히 하였고, 향후 치료프로그램을 구성하는데 중요한 이론적 근거가 될 것으로 기대된다. 마지막으로 본 연구의 제한점, 장래 연구에 대하여 논의하였다.