땅콩짚의 NaOH 처리에 의한 사료가치 개선에 관한 연구

명규호,김용식,선상수 ( K . H . Myung,Y . S . Kim,S . S . Sun ) 한국축산학회 1986 한국축산학회지 Vol.28 No.2

To evaluate the effect of NaOH treatment on peanut straw of the different parts, chemical composition, in vitro dry matter digestibility (IVDMD, %) and in vitro organic matter digestibility (IVOMD, %) were tested. The parts of peanut straw were devided into four parts of blade, stem, hull and whole straw. The results obtained are summarized as follows; 1. The blade contained more crude protein, crude fat, nitrogen free extract and crude ash, but reverse phenomina were observed in the contents of crude fiber and cell wall constituents than the others. 2. In vitro dry matter digestibilities (IVDMD, %) of the four parts of NaOH-treated peanut straw were highly significantly different (p$lt;0.01) in different levels of NaOH treatment and of in vitro times. 3. In vitro organic matter digestibilities (IVOMD, %) of the four parts of peanut straw were showed the same tendency with the IVDMD`s (%).

진정이용 ( 眞正利用 ) 광물질 측정방법에 의한 칼슘이용성 측정에 관한 연구 1 . 산란 메추리의 칼슘요구량 측정

명규호,선상수,김용식 ( K . H . Myung,S . S . Sun,Y . S . Kim ) 한국축산학회 1983 한국축산학회지 Vol.25 No.6

This experiment was conducted to determine the calcium requirement in the determination of calcium availability. Forty breeding Japanese quails of 13 weeks of age were randomly alloted to 10 calcium input levels(0.240,400,480,560,640,720,800,1000 and 1200㎎) with four replicates of one bird each. After the calcium fasting period of 24hr, the calcium unfed group (Omg) was fed the experimental diet to determine the metabolic fecal calcium (FmCa) plus endogeneous urinary calcium (UeCa), however, the calcium fed groups were forcedly fed the known amounts of calcium into the crop and fed the experimental diet afterward for 48hours. The results of the present study were summarized as follows. 1. According to the results of performance during experimental period, body weight, calcium devoid diet intake and excreta weight of tested birds were not significantly affected by the calcium input level. 2. The relationship between calcium excreta and input of 0 to 400㎎ was not showed, however, the highly correlation (P$lt;0.01) was found between calcium excreta and input of 480 to 1200㎎. The correlation equation between calcium excreta and input of 480 to 1200㎎ was Y = 0.9311 X - 463.6012 (r=0.9929**). 3. The maintenance requirement(FmCa+UeCa) of calcium was 0.71㎎ and production plus repletion requirement of that was 497.9 ㎎ for breeding quails. Therefore, the total calcium requirement of a breeding quail was 498.6㎎ in the determination of calcium availability. In conclusion, it could be said that a 72hours experimental period did not affect to body weight, calcium devoided diet intake and excreta weight of tested birds, and that the total requirement was 498.6㎎ in the determination of calcium availability.

암모니아가스 처리 볏짚의 반추위내 소화과정에 대한 주사형 ( 走査型 ) 전자현미경적 연구

명규호(Kyu Ho Myung),김윤수(Yoon Soo Kim),김용식(Yong Sik Kim) 한국축산학회 1987 한국축산학회지 Vol.29 No.11

The experiment was carried out to investigate how ammoniation could affect the digestive patterns and the digestibility of rice straws using in situ technique, and scanning electron and light microscope. It was noted that ammoniation of rice straws significantly improved the digestibility at all digestion times of in situ study. The rice straws consisted of epidermis, sclerenchyma and vascular bundles with tightly packed parenchyma cells, and the stems were surrounded with intact leaf sheaths. Degradation of rice stems began with parenchyma, and proceeded to the vascular bundles and inner cuticle. Sclerenchyma and epidermis resisted degradation more than parenchyma and vascular bundles. The ammoniation during 24 hrs digestion resulted in the total degradation of parenchyma cells, Vascular bundles and inner cuticle in the ammoniated rice straws subjected to 48 hrs in situ digestion were completely digested. However, no further degradation was observed in the ammoniated stems for 72 hrs-incubation, although sclerenchyma cells were slightly broken apart into fiber cells. It is noted that ammoniated stems digested 24 hrs were much more degraded than the control samples for 72 hrs. In addition, LM observations indicated that tissues giving an acid phlorglucinol reaction for lignin appareed to totally resist attack by rumen microbes. Conculsively, ammoniation of rice straws greatly increased the amount of ground parenchyma, vascular bundles and inner cuticle digested, but had little effect on the highly lignified sclerenchyma and epidermis.

육성비육돈의 기별사양이 생산성에 미치는 영향

김광현(K . H . Kim),명규호(K . H . Myung),선상수(S . S . Sun),김상조(S . J . Kim),김재필(J . P . Kim) 한국축산학회 1992 한국축산학회지 Vol.34 No.1

This experiment was conducted to determine the effect of phase feeding on the growth, carcass characteristics and feed cost of pigs. Fifty four weanling pigs (Landrace × Large White × Duroc) weighing about 12.6㎏ were randomly allotted to 3 treatments(treatment Ⅰ : weaner-grower-finisher diets of 9-15-21 wk, treatment Ⅱ: 10-18-21 wk, treatment Ⅲ: 12-21 wk) for feeding trial. Each treatment consisted of 3 replicates and 6 pigs per replicate. Twelve pigs were slaughtered for determining carcass characteristics after the feeding trial. The results obtained are summarized as follows : There were no significant differences(P$lt;0.05) among groups in daily body weight gain, feed consumed, feed conversion and carcass quality. In the analysis of production economy, feed costs required per ㎏ of body weight gain for pigs fed weaner-grower-finisher diets of 9-15-21 wk and 10-18-21 wk were less than that for those fed 12-21 wk(P$lt;0.05).

콩짚의 품종별 사료가치 비교

김용식,명규호,나진수 ( Y . S . Kim,K . H . Myung,J . S . Na ) 한국축산학회 1982 한국축산학회지 Vol.24 No.5

To evaluate the nutritive values of soybean straws of the different varieties, chemical compositions and in vitro digestibilities were examined. The soybean varieties used in this study were Bongeui, Dongbug, Suweon 86, Kwangkyo, Yugoo 3 and Hill. The results obtained are summarized as follows (1) The average weight of soybean straws was 12.0 g. The propotion of pod and stem were 60.4% and 39.6% of the straw fractions on a dry matter basis. (2) Although there were no differences in chemical composition, crude protein was slightly higher in Bongeui and Hill than in others. Pod contained more crude protein, nitrogen free extract, crude ash, cell content and hemicellulose than stem, but reverse phenomena were observed in the contents of cell wall constituents (CWC), acid detergent fiber (ADF), cellulose and lignin. (3) Dry matter, neutral detergent fiber (NDF), ADF and cellulose digestibilities of soybean straws in vitro were highest in Kwangkyo and lowest in Yugoo 3. Dry matter, NDF, ADF and cellulose digestibilities of pod in vitro were higher than those of stem. Eighty percent of the straw fraction, pod and stem based on a dry matter were disappeared within 48 hours by in vitro digestion.

재래닭의 근육 성장과 관련되는 cDNA Clone의 염기서열 및 특성

선상수,명규호,국길,김남오,Sun, S.S.,Myung, K.H.,Kuk, K.,Kim, N.O. 한국가금학회 2006 韓國家禽學會誌 Vol.33 No.4

본 연구는 재래닭의 성장 관련 유용 유전자를 검색하기 위하여, 재래닭에서 발현되는 유전자와 코니쉬에서 발현되는 유전자를 subtraction하여 cDNA library를 구축하고, 염기서열을 밝혀서 재래닭 특이 유용 유전자를 검증하고자 하였다. cDNA library에서 얻은 clone들의 염기서열을 분석하여 나타난 결과를 5개의 clone을 비교 분석하였다. Clone NDS-1(618nt)은 비록 타 종과의 상동성은 낮으나(10%) 해당 과정에서 중요한 역할을 담당하는 triosephosphate isomerase이며, Clone NDS-6(651nt)는 자에서 해당 과정에 관여하는 glyceraldehyde-3-phosphate dehydrogenase로 여겨진다. 그러나 3가지 유전자(clone NDS-2, NDS-10, NDS-12)는 다른 유전자들과 비교하여 5.0%내외의 낮은 상동성을 보이고 고등 동물과 거의 유사성이 없으므로 재래닭 특이 성장 관련 유용 유전자일 가능성이 있다. This experiment was conducted to examine the effective DNA related with muscle growth of Korean native chicken. cDNA library was constructed with mRNA subtraction from Korean native chicken to Cornish. Total mRNA was purified from pectoralis muscle of adult chicken. Five clones were compared their DNA sequence and characteristics based on GenBank. Clone NDS-1 (618nt) was low homology (10%) with other species, but it is closely related with triosephosphate isomerase which is play an important role in glycolysis. Clone NDS-6 (651nt) is corresponding to glyceraldehyde-3-phosphate dehydrogenase. These two clones are encoding to enzymes in key role in glycolysis. However, other three clones (NDS-2, NDS-10, NDS-12) have low homology with other species about 5.0%. These clones were not similar with any other eukaryotics. Therefore, three clones (NDS-2, NDS-10, NDS-12) are high possibility of specific DNA for muscle growth in Korean native chicken.

칼슘급여수준· 급여방법·입자도·연령이 메추리의 진정이용칼슘가에 미치는 영향

김용식,선상수,명규호 ( Y . S . Kim,S . S . Sun,K . H . Myung ) 한국축산학회 1985 한국축산학회지 Vol.27 No.6

The relationships between calcium input level and excreta calcium, and true available calcium (TAC) values were Y=3.21 × 10^(-4)x²+ 0.52X-0.48 (r=0.9832^(**) and Y=-6.21 × 10^(-3)X² -0.03 (r=0.9738^(**)) respectively. The TAC values of limestone and oyster shell were the highest in the 40-80 mesh as 74.67%, and the lowest in the 10-30 mesh as 68.2% and 61.01% respectively. Excreted calcium from fed birds were significantly increased (p$lt;0.05) as the age of birds increased, however, excreted calcium from unfed birds were almost same in all the age of birds. The TAC value of calcium carbonate was the highest in that of 16 weeks old bird as 99.42±1.32%. The relationships between age of bird and excreta calcium, and TAC value were Y=0.578x-0.0475 (r=0.9960^(**)) and Y=-0.129X+100.0425 (r=-0.9850^(**)) respectively.

국산 인산칼슘제의 사료적 가치에 관한 연구 2 . 저수준의 어분급여시 인산칼슘제가 부로일러의 성장능력 및 경골내 칼슘·인의 축적에 미치는 영향

한인규,오상집,명규호,박종만,김춘수 ( In K . Han,Sang J . Ohh,Kyu H . Myung,Jong M . Park,Chun S . Kim ) 한국축산학회 1978 한국축산학회지 Vol.20 No.6

In order to compare the utilizability of calcium and phosphorus from locally produced tricalcium phosphate (TCP) with that of the imported TCP, dicalcium phosphate (DCP) or bone meal when fed with small amount of fish meal 360 broiler-type chicks of one day old were used for 6 treatments consisted of Control Ⅰ, Control Ⅱ, Imported TCP. Local TCP, Improted DCP and Bone meal group. The results obtained during 5 weeks of experimental period are summarized as follows. 1. It was found that; the body weight gain for birds received various sources of inelegancies phosphates was significantly (P$lt;0.05) higher than for birds received no phosphate or 4% fish meal (Control Ⅱ group). Control Ⅱ group also gained more body weight than Control Ⅰ group. Feeding local tricalcium phosphate, imported dicalcium phosphate and bone meal could significantly (P$lt;0.05) Improved the feed efficiency. 2. It was evidenced that feed intake for Control Ⅰ group was significantly (P$lt;0.05) depressed than for other groups. Although no statistical significances were found among other treatments, the amount of feed intake were tended to increase by the use of calcium phosphate supplements. 3. No difference was observed in nutrient utilizabilities either among treatments or between control and treated groups. 4. The tibia ash. content of broilers fed control Ⅰ diet was significantly (P$lt;0.05) lower than that of others. It was also found that tibia ash content of broilers fed control Ⅱ diet was higher than that of control Ⅰ group. Calcium and phosphorus content of tibia was also higher for the broilers fed various phosphate supplements than control groups as was shown in the case of tibia ash content. 5. Present data indicated that the mortality of broilers during experimental period increased as the level of phosphorous in diets decreased and Ca : P ratio was widened. The results obtained from the present experiments showed that the feeding value of locally produced tricalcium phosphate was comparable to that of imported tricalcium phosphate in body weight gain, feed efficiency, mortality and tibia content of ash, calcium and phosphorus.

메추리의 6개 칼슘공급원에 따른 진정이용칼슘과 외견상이용칼슘가의 비교

김용식,선상수,명규호 ( Y . S . Kim,S . S . Sun,K . H . Myung ) 한국축산학회 1985 한국축산학회지 Vol.27 No.5

True available calcium(TAC) values of 6 calcium supplements were a little greater than apparent available calcium(AAC) value of them, however, no significant difference was found between two kinds of available calcium values. There were significant differences (p$lt;0.01) among each TAC and AAC values of 6 calcium supplements. TAC values of calcium carbonate and oyster shell in mixed diets were significantly (p$lt;0.05) greater then AAC values of them. TAC and AAC values of calcium carbonate were the highest as 99.94%±2.10 and 98.45%±1.07, and those of oyster shell were the lowest as 67.78%±1.96 and 67.40%±2.07 respectively.

과산화수소처리 볏짚의 반추가축 사료화에 관한 연구

김용식(Y . S . Kim),명규호(K . H . Myung),권동석(D . S . Kwon),이석래(S . R . Lee),이태훈(T . H . Lee) 한국축산학회 1990 한국축산학회지 Vol.32 No.3

Experiments were carried out to investigate on the effect of cryoprotectants of the quality changes of chicken breast and leg meat during frozen storage. Chicken breast and leg meat were mixed with various cryoprotectants and stored at -20℃ in a chest freezer for 12 weeks. Samples were analyzed for pH changes, TBA value, free fatty acid contents, water and salt soluble protein extractability. The results obtained are summerized as follows ; 1. The pH value in all of cryoprotectants added samples were increased up to 0.27-0.46 in chicken breast and 0.1-0.25 in leg meat as in non treated samples. The TBA value were increased with storage time and higher value at 12 weeks as compared with the non-treated samples. 2. The effect of cryoprotectants on the free fatty acid of chicken meat were showed significance. The cryoprotectant effect on the free fatty acid was greater in chicken leg than in breast meat, especially CP-C treated samples. 3. The effect of cryoprotectants on the water soluble protein extractability were showed no significance. 4. The cryoprotectant effect on the salt soluble protein extractability was more pronounced in chicken leg than in breast meat. The extractability of the CP-A treated in chicken leg meat was increased up to 20-50% as in non treated samples.