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한우의 근출혈 발생 상태에 따른 혈액내 포도당, Creatinine, Lactate, Cortisol 수준과 근육섬유의 조직학적 분석
선상수,이성민 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2017 동물자원연구 Vol.28 No.3
This study investigated the stress-related metabolites and hormones in blood and compared the muscle structure to identify the reason for blood splash in Hanwoo beef. Five slaughter houses were selected based on the region (Seoul, Gimhae, Jungbu, Naju, and Goryung) and a previous blood splash record. In total, three-hundred eighty blood samples (n=380) and forty-two muscle tissues (n=42) of control and blood splash Hanwoo beef were collected during the slaughter process and beef grading. Blood metabolites were analyzed including glucose, lactate, creatinine, urea-N, and hormones such as cortisol and thyroxin. Muscle fiber, fiber bundle, and capillary wall thickness were measured under microscope. The concentrations of blood glucose, lactate, and urea-N were not significantly different between the control and the blood splash samples. Cortisol and thyroxin levels were not significantly different in both samples. In contrast, the creatinine level was significantly increased (p<0.05) in the blood splash samples. There were also no significant differences observed in muscle fiber, bundles, and capillary wall thickness between the control and the blood splash tissues. In conclusion, blood metabolites, hormones, and muscle fiber showed no differences between the control and the blood splash animal. However, increased creatinine levels may be used as an indicator for identifying blood splash prior to slaughter in Hanwoo.
비육돈에 발아보리와 청보리 혼합 첨가사료 급여가 소화율과 도체등급에 미치는 영향
선상수,정용수,이현삼,김경현 全南大學校 農業科學技術硏究所 2016 農業科學技術硏究 Vol.53 No.-
One-hundred twenty six "Golden Pigs" (YxBxD three-way crossbreed, 135-day old, 75~80kg BW) were randomly assigned each 42 pigs in three treatment groups. Three treatments were control, treatment 1 (10%VAW + 2% green barley straw), and treatment 2 (10%VAW + 4% GBS). Experimental diets were mixed commercial diet with vitalised activated white (VAW) and green barley straw(GBS) and then fed 45 days. Measurements were feed intake, weight gain, and digestibility. Meat grade were measures from "Korea Institute for Animal Products Quality Evaluation, KAPE". ADG was significantly (p<0.05) decreased in treatment 1 (533g) and treatment 2 (470g) that in control group. Meat panel test were control (36%), treatment 1 (28%), treatment 2 (36%). Back fat was low in treatment 1. Meat grade were control (1.125), treatment 1 (0.875), and treatment 2 (0.667). Therefore meat guality was improved by treatment. In result, VAW and green barley straw addition was improved meat quality without other detrimental effects in finishing pig.
한우 및 비육우의 출하지역별, 성별, 및 축종별 결함육 발생 현황 비교
선상수,장선식,강태종 전남대학교 농업과학기술연구소 2017 농업생명과학연구동향 Vol.54 No.
ABSTRACT Blood splash and carcass defects were analyzed from beef cattle in Seoul slaughter house and Hanwoo in nationwide all slaughter houses from 2003 to 2007. Carcass defects was analyzed by carcass grade (70,982 cattle) from different marketing province (Seoul-Gyunggi, Chung-nam, Chung-buk, Jeon-buk, Jeon-nam, Gyung-buk, Gyung-nam), sexes (male, female, steer), and breed (33,000 Hanwoo, 7,000 dairy cattle, 37,000 beef cattle). Data were analyzed not by the statistical differences but differences by items and years. Slaughtered cattle in Seoul slaughter house were came from Seoul-Kyunggi (37.8%), Chung Nam-Buk (28.5%), Kyung Buk (11.5%) and rest of province. Incidence of blood splash in Seoul slaughter house was 1.14% in Hanwoo, 0.72% in dairy cattle, and 2.96% in beef cattle and it was the highest in steers. In general, blood splash in nationwide was 0.26~0.32% in Hanwoo, and then it was 80.1~85.5% of carcass defects. This incidence was increased in 2006. However, intrinsic and extrinsic factors, such as transport method, distance, animal density etc, may effect on blood splash in Hanwoo. In conclusion, blood splash would be controlled by the manipulation of beef cattle management during transportation. Blood splash incidence was different among marketing province to Seoul slaughter house, and all carcass defects was increased gradualy during five years in nationwide.
비전분성 탄수화물 분해효소 혼합급여가 육계의 생산성, 혈중 대사물질, 및 장내미생물의 변화에 미치는 영향
선상수,박철주 한국산학기술학회 2019 한국산학기술학회논문지 Vol.20 No.12
This study examined the productivity, blood characteristics, and cecal microorganisms with the addition of mixed enzymes in broiler chickens. Three hundred chickens (Ross 308) were assigned randomly to five treatment groups (control, 0.5 MXG, 1.0 MXG, 2.0 MXG, and 1.0 G) with three replications. Based on the results, the weight, feed intake, feed efficiency, and daily gain increased slightly by the treatment assignment. Carcass, stomach, and heart weights increased slightly in all treatment groups compared to the control. On the other hand, liver weight was significantly low by enzyme addition compared to the control group (p<0.05). The total number of appendix bacteria decreased significantly in all treatment groups compared to the control group (p<0.05). The total protein, blood urea nitrogen (BUN), and glucose level did not differ after treatment. On the other hand, the IgG level was significantly higher in the 1.0 MXG and 2.0 MXG groups than in the control group (p<0.05). In conclusion, the addition of a mixed enzyme (MXG) will improve the feed efficiency and IgG, as well as reduce the liver weight and total bacteria in broiler chickens. 본 연구는 혼합효소제를 육계사료에 첨가 급여 하였을때 생산성, 혈액성분, 및 장내 미생물의 변화를 구명하고자 실시하였다. 병아리(Ross 308) 300수를 공시하여 5개의 처리구 (대조구, 0.5MXG, 1.0MXG, 2.0MXG 와 1.0G)에 완전임의배치하고 3반복으로 수행하였다. 결과는 체중, 사료섭취량, 사료효율 및 일당증체량은 대조구에 비하여 MXG 효소 처리구에서 모두 높게 나타났다. 도체중, 근위, 심장의 무게는 대조구에 비하여 효소 첨가구에서 높게 측정되었으나, 이는 도체중의 증가에 대한 영향으로 보인다. 그러나, 간장의 무게는 대조구에 비하여 처리구에서 유의적으로 낮게 측정되었다 (p<0.05). 장내 전체 세균수는 대조구에 비하여 효소 첨가구(MXG)에서 유의적으로 감소하였다 (p<0.05). 혈액성분 중에서 total protein, 혈중요소태질소(BUN) 와 포도당 함량은 변화가 없었다. 그러나, 면역항체인 IgG 농도는 대조구에 비하여 1.0 과 2.0 MXG 혼합 급여구에서 유의적으로 높았다 (p<0.05). 결론적으로 혼합효소제 첨가는 육계에서 사료효율과 IgG 수준은 향상시켰으며, 간장의 무게와 장내 세균숫자는 감소시키는 효과가 있다.
산란계의 뇌하수체 세포배양에서 Prolactin의 생성에 관계하는 Protein Kinase C의 역할
선상수 한국가금학회 1996 韓國家禽學會誌 Vol.23 No.3
A series of experiments were conducted to investigate the role of protein kinase C (PKC) as a second messenger in vasoactive intestinal peptide (VIP) mediated prolactin secretion. Primary pituitary cells (106 cells/treatment) were separated from laying hens and incubated in M-199 with 5% chicken serum and 5% fetal calf serum. The VIP(0.1 $\pi$M) treatment enhanced prolactin Secretion into media upto 9-fold during 48-h incubation. The phorbol 12-myristate 13-acetate (PMA), a PKG agonist, increased prolactin secretion upto 2-fold at 0.1 nM PMA (P<0.01), and the prolactin secretion was not significantly higher than this concentration. Staurosporine (ST; 1.0$\pi$M) a PKC antagonist, decreased by 70% of 0.1 $\pi$M VIP-stimulated prolactin secretion and by 48% of 10 ${\mu}$M PMA-stimulated prolactin secretion (P<0.01). However, pituitary cell prolactin content did not differ in any treatment (P>0.05). In conclusion, these results indicate that the PKC second messenger system is involved in VIP-stimulated prolactin release in chicken primary pituitary cell culture.
Mannanase와 Xylanase 첨가급여에 따른 육계의 생산성, 혈중대사물질, 맹장내 미생물, 및 계분악취에 미치는 영향
선상수,박철주 한국산학기술학회 2024 한국산학기술학회논문지 Vol.25 No.3
본 연구는 mannanase (M)와 xylanase (X)를 육계사료에 첨가 급여 하였을때 생산성, 혈액성분, 장내 미생물, 및 분뇨악취에 미치는 영향을 구명하고자 실시하였다. 병아리 (Ross 308) 300수를 공시하여 5개의 처리구(대조구, M1.0, M2.0, X1.0, X2.0)에 완전 임의배치하고 3반복으로 수행하였다. 효소제는 mannanase (200U/kg) 와 xylanase (1,000U/kg)을 기준으로 사료내 1.0배, 2.0배 사료에 혼합 급여하였다. 결과는 생체중, 사료섭취량, 일당증체량, 및 생산지수에는 변화가 없었으나 사료효율은 M2.0, X1.0 과 X2.0 급여구에서 유의적 (p <0.05)으로높게 측정되었다. 또한 간장과 맹장의 무게는 대조구에 비하여 효소제 급여구에서 유의적 (p <0.05)으로 높게 측정되었다. 전체 세균수는 증가하고 E. Coli 와 Salmonella는 모두 대조구에 비하여 효소 급여구에서 감소하는 경향을 보였으나, 장내세균에는 유의차가 나타나지 않았다. 혈액성분 중에서 단백질, 혈중요소태질소 와 포도당 함량은변화가 없었으나, 면역항체인 IgG 농도는 대조구에 비하여 M2.0 과 X2.0 급여구에서 유의적으로 높았다 (p <0.05). 또한, 계분내 암모니아 발생량은 대조구 대비 M2.0 과 X2.0 급여구에서 유의적으로 낮았다 (p <0.05). 결론적으로mannanase 와 xylanase 첨가 급여는 육계에서 사료효율과 IgG 수준을 향상시켰고, 간장과 맹장의 무게가 증진되었으며, 계분 암모니아 발생량을 감소시키는 효과가 있다.