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한우의 근출혈 발생 상태에 따른 혈액내 포도당, Creatinine, Lactate, Cortisol 수준과 근육섬유의 조직학적 분석
선상수,이성민 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2017 동물자원연구 Vol.28 No.3
This study investigated the stress-related metabolites and hormones in blood and compared the muscle structure to identify the reason for blood splash in Hanwoo beef. Five slaughter houses were selected based on the region (Seoul, Gimhae, Jungbu, Naju, and Goryung) and a previous blood splash record. In total, three-hundred eighty blood samples (n=380) and forty-two muscle tissues (n=42) of control and blood splash Hanwoo beef were collected during the slaughter process and beef grading. Blood metabolites were analyzed including glucose, lactate, creatinine, urea-N, and hormones such as cortisol and thyroxin. Muscle fiber, fiber bundle, and capillary wall thickness were measured under microscope. The concentrations of blood glucose, lactate, and urea-N were not significantly different between the control and the blood splash samples. Cortisol and thyroxin levels were not significantly different in both samples. In contrast, the creatinine level was significantly increased (p<0.05) in the blood splash samples. There were also no significant differences observed in muscle fiber, bundles, and capillary wall thickness between the control and the blood splash tissues. In conclusion, blood metabolites, hormones, and muscle fiber showed no differences between the control and the blood splash animal. However, increased creatinine levels may be used as an indicator for identifying blood splash prior to slaughter in Hanwoo.
산란계의 뇌하수체 세포배양에서 Prolactin의 생성에 관계하는 Protein Kinase C의 역할
선상수 한국가금학회 1996 韓國家禽學會誌 Vol.23 No.3
A series of experiments were conducted to investigate the role of protein kinase C (PKC) as a second messenger in vasoactive intestinal peptide (VIP) mediated prolactin secretion. Primary pituitary cells (106 cells/treatment) were separated from laying hens and incubated in M-199 with 5% chicken serum and 5% fetal calf serum. The VIP(0.1 $\pi$M) treatment enhanced prolactin Secretion into media upto 9-fold during 48-h incubation. The phorbol 12-myristate 13-acetate (PMA), a PKG agonist, increased prolactin secretion upto 2-fold at 0.1 nM PMA (P<0.01), and the prolactin secretion was not significantly higher than this concentration. Staurosporine (ST; 1.0$\pi$M) a PKC antagonist, decreased by 70% of 0.1 $\pi$M VIP-stimulated prolactin secretion and by 48% of 10 ${\mu}$M PMA-stimulated prolactin secretion (P<0.01). However, pituitary cell prolactin content did not differ in any treatment (P>0.05). In conclusion, these results indicate that the PKC second messenger system is involved in VIP-stimulated prolactin release in chicken primary pituitary cell culture.
비전분성 탄수화물 분해효소 혼합급여가 육계의 생산성, 혈중 대사물질, 및 장내미생물의 변화에 미치는 영향
선상수,박철주 한국산학기술학회 2019 한국산학기술학회논문지 Vol.20 No.12
This study examined the productivity, blood characteristics, and cecal microorganisms with the addition of mixed enzymes in broiler chickens. Three hundred chickens (Ross 308) were assigned randomly to five treatment groups (control, 0.5 MXG, 1.0 MXG, 2.0 MXG, and 1.0 G) with three replications. Based on the results, the weight, feed intake, feed efficiency, and daily gain increased slightly by the treatment assignment. Carcass, stomach, and heart weights increased slightly in all treatment groups compared to the control. On the other hand, liver weight was significantly low by enzyme addition compared to the control group (p<0.05). The total number of appendix bacteria decreased significantly in all treatment groups compared to the control group (p<0.05). The total protein, blood urea nitrogen (BUN), and glucose level did not differ after treatment. On the other hand, the IgG level was significantly higher in the 1.0 MXG and 2.0 MXG groups than in the control group (p<0.05). In conclusion, the addition of a mixed enzyme (MXG) will improve the feed efficiency and IgG, as well as reduce the liver weight and total bacteria in broiler chickens. 본 연구는 혼합효소제를 육계사료에 첨가 급여 하였을때 생산성, 혈액성분, 및 장내 미생물의 변화를 구명하고자 실시하였다. 병아리(Ross 308) 300수를 공시하여 5개의 처리구 (대조구, 0.5MXG, 1.0MXG, 2.0MXG 와 1.0G)에 완전임의배치하고 3반복으로 수행하였다. 결과는 체중, 사료섭취량, 사료효율 및 일당증체량은 대조구에 비하여 MXG 효소 처리구에서 모두 높게 나타났다. 도체중, 근위, 심장의 무게는 대조구에 비하여 효소 첨가구에서 높게 측정되었으나, 이는 도체중의 증가에 대한 영향으로 보인다. 그러나, 간장의 무게는 대조구에 비하여 처리구에서 유의적으로 낮게 측정되었다 (p<0.05). 장내 전체 세균수는 대조구에 비하여 효소 첨가구(MXG)에서 유의적으로 감소하였다 (p<0.05). 혈액성분 중에서 total protein, 혈중요소태질소(BUN) 와 포도당 함량은 변화가 없었다. 그러나, 면역항체인 IgG 농도는 대조구에 비하여 1.0 과 2.0 MXG 혼합 급여구에서 유의적으로 높았다 (p<0.05). 결론적으로 혼합효소제 첨가는 육계에서 사료효율과 IgG 수준은 향상시켰으며, 간장의 무게와 장내 세균숫자는 감소시키는 효과가 있다.
비육돈에 발아보리와 청보리 혼합 첨가사료 급여가 소화율과 도체등급에 미치는 영향
선상수,정용수,이현삼,김경현 全南大學校 農業科學技術硏究所 2016 農業科學技術硏究 Vol.53 No.-
One-hundred twenty six "Golden Pigs" (YxBxD three-way crossbreed, 135-day old, 75~80kg BW) were randomly assigned each 42 pigs in three treatment groups. Three treatments were control, treatment 1 (10%VAW + 2% green barley straw), and treatment 2 (10%VAW + 4% GBS). Experimental diets were mixed commercial diet with vitalised activated white (VAW) and green barley straw(GBS) and then fed 45 days. Measurements were feed intake, weight gain, and digestibility. Meat grade were measures from "Korea Institute for Animal Products Quality Evaluation, KAPE". ADG was significantly (p<0.05) decreased in treatment 1 (533g) and treatment 2 (470g) that in control group. Meat panel test were control (36%), treatment 1 (28%), treatment 2 (36%). Back fat was low in treatment 1. Meat grade were control (1.125), treatment 1 (0.875), and treatment 2 (0.667). Therefore meat guality was improved by treatment. In result, VAW and green barley straw addition was improved meat quality without other detrimental effects in finishing pig.
한우 및 비육우의 출하지역별, 성별, 및 축종별 결함육 발생 현황 비교
선상수,장선식,강태종 전남대학교 농업과학기술연구소 2017 농업생명과학연구동향 Vol.54 No.
ABSTRACT Blood splash and carcass defects were analyzed from beef cattle in Seoul slaughter house and Hanwoo in nationwide all slaughter houses from 2003 to 2007. Carcass defects was analyzed by carcass grade (70,982 cattle) from different marketing province (Seoul-Gyunggi, Chung-nam, Chung-buk, Jeon-buk, Jeon-nam, Gyung-buk, Gyung-nam), sexes (male, female, steer), and breed (33,000 Hanwoo, 7,000 dairy cattle, 37,000 beef cattle). Data were analyzed not by the statistical differences but differences by items and years. Slaughtered cattle in Seoul slaughter house were came from Seoul-Kyunggi (37.8%), Chung Nam-Buk (28.5%), Kyung Buk (11.5%) and rest of province. Incidence of blood splash in Seoul slaughter house was 1.14% in Hanwoo, 0.72% in dairy cattle, and 2.96% in beef cattle and it was the highest in steers. In general, blood splash in nationwide was 0.26~0.32% in Hanwoo, and then it was 80.1~85.5% of carcass defects. This incidence was increased in 2006. However, intrinsic and extrinsic factors, such as transport method, distance, animal density etc, may effect on blood splash in Hanwoo. In conclusion, blood splash would be controlled by the manipulation of beef cattle management during transportation. Blood splash incidence was different among marketing province to Seoul slaughter house, and all carcass defects was increased gradualy during five years in nationwide.
재래닭의 근육 성장과 관련되는 cDNA Clone의 염기서열 및 특성
선상수,명규호,국길,김남오,Sun, S.S.,Myung, K.H.,Kuk, K.,Kim, N.O. 한국가금학회 2006 韓國家禽學會誌 Vol.33 No.4
본 연구는 재래닭의 성장 관련 유용 유전자를 검색하기 위하여, 재래닭에서 발현되는 유전자와 코니쉬에서 발현되는 유전자를 subtraction하여 cDNA library를 구축하고, 염기서열을 밝혀서 재래닭 특이 유용 유전자를 검증하고자 하였다. cDNA library에서 얻은 clone들의 염기서열을 분석하여 나타난 결과를 5개의 clone을 비교 분석하였다. Clone NDS-1(618nt)은 비록 타 종과의 상동성은 낮으나(10%) 해당 과정에서 중요한 역할을 담당하는 triosephosphate isomerase이며, Clone NDS-6(651nt)는 자에서 해당 과정에 관여하는 glyceraldehyde-3-phosphate dehydrogenase로 여겨진다. 그러나 3가지 유전자(clone NDS-2, NDS-10, NDS-12)는 다른 유전자들과 비교하여 5.0%내외의 낮은 상동성을 보이고 고등 동물과 거의 유사성이 없으므로 재래닭 특이 성장 관련 유용 유전자일 가능성이 있다. This experiment was conducted to examine the effective DNA related with muscle growth of Korean native chicken. cDNA library was constructed with mRNA subtraction from Korean native chicken to Cornish. Total mRNA was purified from pectoralis muscle of adult chicken. Five clones were compared their DNA sequence and characteristics based on GenBank. Clone NDS-1 (618nt) was low homology (10%) with other species, but it is closely related with triosephosphate isomerase which is play an important role in glycolysis. Clone NDS-6 (651nt) is corresponding to glyceraldehyde-3-phosphate dehydrogenase. These two clones are encoding to enzymes in key role in glycolysis. However, other three clones (NDS-2, NDS-10, NDS-12) have low homology with other species about 5.0%. These clones were not similar with any other eukaryotics. Therefore, three clones (NDS-2, NDS-10, NDS-12) are high possibility of specific DNA for muscle growth in Korean native chicken.