재래닭의 근육 성장과 관련되는 cDNA Clone의 염기서열 및 특성

선상수,명규호,국길,김남오,Sun, S.S.,Myung, K.H.,Kuk, K.,Kim, N.O. 한국가금학회 2006 韓國家禽學會誌 Vol.33 No.4

본 연구는 재래닭의 성장 관련 유용 유전자를 검색하기 위하여, 재래닭에서 발현되는 유전자와 코니쉬에서 발현되는 유전자를 subtraction하여 cDNA library를 구축하고, 염기서열을 밝혀서 재래닭 특이 유용 유전자를 검증하고자 하였다. cDNA library에서 얻은 clone들의 염기서열을 분석하여 나타난 결과를 5개의 clone을 비교 분석하였다. Clone NDS-1(618nt)은 비록 타 종과의 상동성은 낮으나(10%) 해당 과정에서 중요한 역할을 담당하는 triosephosphate isomerase이며, Clone NDS-6(651nt)는 자에서 해당 과정에 관여하는 glyceraldehyde-3-phosphate dehydrogenase로 여겨진다. 그러나 3가지 유전자(clone NDS-2, NDS-10, NDS-12)는 다른 유전자들과 비교하여 5.0%내외의 낮은 상동성을 보이고 고등 동물과 거의 유사성이 없으므로 재래닭 특이 성장 관련 유용 유전자일 가능성이 있다. This experiment was conducted to examine the effective DNA related with muscle growth of Korean native chicken. cDNA library was constructed with mRNA subtraction from Korean native chicken to Cornish. Total mRNA was purified from pectoralis muscle of adult chicken. Five clones were compared their DNA sequence and characteristics based on GenBank. Clone NDS-1 (618nt) was low homology (10%) with other species, but it is closely related with triosephosphate isomerase which is play an important role in glycolysis. Clone NDS-6 (651nt) is corresponding to glyceraldehyde-3-phosphate dehydrogenase. These two clones are encoding to enzymes in key role in glycolysis. However, other three clones (NDS-2, NDS-10, NDS-12) have low homology with other species about 5.0%. These clones were not similar with any other eukaryotics. Therefore, three clones (NDS-2, NDS-10, NDS-12) are high possibility of specific DNA for muscle growth in Korean native chicken.

메추리의 6개 칼슘공급원에 따른 진정이용칼슘과 외견상이용칼슘가의 비교

김용식,선상수,명규호 ( Y . S . Kim,S . S . Sun,K . H . Myung ) 한국축산학회 1985 한국축산학회지 Vol.27 No.5

True available calcium(TAC) values of 6 calcium supplements were a little greater than apparent available calcium(AAC) value of them, however, no significant difference was found between two kinds of available calcium values. There were significant differences (p$lt;0.01) among each TAC and AAC values of 6 calcium supplements. TAC values of calcium carbonate and oyster shell in mixed diets were significantly (p$lt;0.05) greater then AAC values of them. TAC and AAC values of calcium carbonate were the highest as 99.94%±2.10 and 98.45%±1.07, and those of oyster shell were the lowest as 67.78%±1.96 and 67.40%±2.07 respectively.

칼슘급여수준· 급여방법·입자도·연령이 메추리의 진정이용칼슘가에 미치는 영향

김용식,선상수,명규호 ( Y . S . Kim,S . S . Sun,K . H . Myung ) 한국축산학회 1985 한국축산학회지 Vol.27 No.6

The relationships between calcium input level and excreta calcium, and true available calcium (TAC) values were Y=3.21 × 10^(-4)x²+ 0.52X-0.48 (r=0.9832^(**) and Y=-6.21 × 10^(-3)X² -0.03 (r=0.9738^(**)) respectively. The TAC values of limestone and oyster shell were the highest in the 40-80 mesh as 74.67%, and the lowest in the 10-30 mesh as 68.2% and 61.01% respectively. Excreted calcium from fed birds were significantly increased (p$lt;0.05) as the age of birds increased, however, excreted calcium from unfed birds were almost same in all the age of birds. The TAC value of calcium carbonate was the highest in that of 16 weeks old bird as 99.42±1.32%. The relationships between age of bird and excreta calcium, and TAC value were Y=0.578x-0.0475 (r=0.9960^(**)) and Y=-0.129X+100.0425 (r=-0.9850^(**)) respectively.

진정이용 ( 眞正利用 ) 광물질 측정방법에 의한 칼슘이용성 측정에 관한 연구 1 . 산란 메추리의 칼슘요구량 측정

명규호,선상수,김용식 ( K . H . Myung,S . S . Sun,Y . S . Kim ) 한국축산학회 1983 한국축산학회지 Vol.25 No.6

This experiment was conducted to determine the calcium requirement in the determination of calcium availability. Forty breeding Japanese quails of 13 weeks of age were randomly alloted to 10 calcium input levels(0.240,400,480,560,640,720,800,1000 and 1200㎎) with four replicates of one bird each. After the calcium fasting period of 24hr, the calcium unfed group (Omg) was fed the experimental diet to determine the metabolic fecal calcium (FmCa) plus endogeneous urinary calcium (UeCa), however, the calcium fed groups were forcedly fed the known amounts of calcium into the crop and fed the experimental diet afterward for 48hours. The results of the present study were summarized as follows. 1. According to the results of performance during experimental period, body weight, calcium devoid diet intake and excreta weight of tested birds were not significantly affected by the calcium input level. 2. The relationship between calcium excreta and input of 0 to 400㎎ was not showed, however, the highly correlation (P$lt;0.01) was found between calcium excreta and input of 480 to 1200㎎. The correlation equation between calcium excreta and input of 480 to 1200㎎ was Y = 0.9311 X - 463.6012 (r=0.9929**). 3. The maintenance requirement(FmCa+UeCa) of calcium was 0.71㎎ and production plus repletion requirement of that was 497.9 ㎎ for breeding quails. Therefore, the total calcium requirement of a breeding quail was 498.6㎎ in the determination of calcium availability. In conclusion, it could be said that a 72hours experimental period did not affect to body weight, calcium devoided diet intake and excreta weight of tested birds, and that the total requirement was 498.6㎎ in the determination of calcium availability.

땅콩짚의 NaOH 처리에 의한 사료가치 개선에 관한 연구

명규호,김용식,선상수 ( K . H . Myung,Y . S . Kim,S . S . Sun ) 한국축산학회 1986 한국축산학회지 Vol.28 No.2

To evaluate the effect of NaOH treatment on peanut straw of the different parts, chemical composition, in vitro dry matter digestibility (IVDMD, %) and in vitro organic matter digestibility (IVOMD, %) were tested. The parts of peanut straw were devided into four parts of blade, stem, hull and whole straw. The results obtained are summarized as follows; 1. The blade contained more crude protein, crude fat, nitrogen free extract and crude ash, but reverse phenomina were observed in the contents of crude fiber and cell wall constituents than the others. 2. In vitro dry matter digestibilities (IVDMD, %) of the four parts of NaOH-treated peanut straw were highly significantly different (p$lt;0.01) in different levels of NaOH treatment and of in vitro times. 3. In vitro organic matter digestibilities (IVOMD, %) of the four parts of peanut straw were showed the same tendency with the IVDMD`s (%).

BOVINE SOMATOTROPIN 투여에 의한 젖소의 유생산성 예측

명규호(K . H . Myung),문승주(S . J . Moon),선상수(S . S . Sun) 한국영양사료학회 1991 韓國營養飼料學會誌 Vol.15 No.5

육성비육돈의 기별사양이 생산성에 미치는 영향

김광현(K . H . Kim),명규호(K . H . Myung),선상수(S . S . Sun),김상조(S . J . Kim),김재필(J . P . Kim) 한국축산학회 1992 한국축산학회지 Vol.34 No.1

This experiment was conducted to determine the effect of phase feeding on the growth, carcass characteristics and feed cost of pigs. Fifty four weanling pigs (Landrace × Large White × Duroc) weighing about 12.6㎏ were randomly allotted to 3 treatments(treatment Ⅰ : weaner-grower-finisher diets of 9-15-21 wk, treatment Ⅱ: 10-18-21 wk, treatment Ⅲ: 12-21 wk) for feeding trial. Each treatment consisted of 3 replicates and 6 pigs per replicate. Twelve pigs were slaughtered for determining carcass characteristics after the feeding trial. The results obtained are summarized as follows : There were no significant differences(P$lt;0.05) among groups in daily body weight gain, feed consumed, feed conversion and carcass quality. In the analysis of production economy, feed costs required per ㎏ of body weight gain for pigs fed weaner-grower-finisher diets of 9-15-21 wk and 10-18-21 wk were less than that for those fed 12-21 wk(P$lt;0.05).

서방형 Bovine Somatotropin 투여에 의한 젖소의 홀몬 , 대사물질 및 장관 운동성의 변화

권동석(D . S . Kwon),김용식(Y . S . Kim),명규호(K . H . Myung),선상수(S . S . Sun) 한국축산학회 1991 한국축산학회지 Vol.33 No.9

The present study was conducted to evaluate the improvement of dairy performance of SR-BST (750 ㎎/head/14 d) using 6 lactating Holstein cows for 42 days. 3.5% FCM was significantly (P$lt;0.05) increased with SR-BST treatment while DM intake and feed efficiency were not greatly altered. Although glucose concentration in serum was not changed with treatment, NEFA concentration was increased twofold and uremia was found in BST cows. Serum concentration of BST was maintained higher by 7 days while that f IGF-I was kept higher ?-ing entire experimental period with BST treatment. However., no difference was found in insuim concentration. There was no difference in nutrient digestibility and gastro-intestial motility between treatments Although there was slightly higher percentage of milk fat, no great difference was found in milk composition with BST treatment. Therefore, it could be concluded from this study that SR-BST improved dairy performance through homeorhetic effect (nutrient repartitioning toward mammary gland) without altering nutrient digestibility and gastro-intestinal motility.