단백질 염산 가수분해 조미액의 제조중 chloropropanols 생성 억제 공정 개발

류형호 慶星大學校 1998 論文集 Vol.19 No.2

Protein hydrolyzates by hydrochloric acid widely used as seasonings and sensory flavours. Protein hydrolyzates contained mutagens of chloropanols such as monochloropropanol, dichloropropanols. This study have now been developed for the chloropropanols reduction using modified bydrolyzed processing. The Ist was performed to adjust the range of 0.5∼1.0 in mole rate of hydrochloric acid/protein-N at 95∼98℃ for 5 days, 2nd process hydrolyzed the ranges of 0.5∼1.0 in mole ratio HCl/protein-N and adjust with pH 8.8 for 2 day. The final 3rd process filterated and adjusted pH 5.2. The modified process maintained to reduce the chlorophenols of protein hydrolyzates prepared by hydrochloric acid.

화학적 지연 처치가 동맥화된 정맥피판의 생존에 미치는 영향

류형호,조병채,변진석,이정형,백봉수 大韓成形外科學會 1996 Archives of Plastic Surgery Vol.23 No.4

Although a free tissue transfer is a well established technique for reconstruction of tissue defects, available free flaps are limited and often associated with significant donor site morbidity. Flaps based on venous trees would greatly increase the selection and diversity of tissue available for reconstruction. A new concept of an arterialized venous flap was first introduced by Nakayama in 1981 using the epigastric flap model of a rat. Since then, free small to moderately sized arterialized venous flap transfers have been applied clinically with increasing frequency in the fields of hand surgery, traumatology, and so on. But large sized flaps are less predictable in their survival. Our previous experiment, demonstrated that the surgical delay improved the arteralized venous flap survival and the optimal delay period, to obtain maximal survival was 2 weeks. The aim of this study was to investigate the effects of chemical delay and the possibillity to reduce the 2 weeks of surgical delay period. Thirty New Zealand white rabbits were randomized into three groups; 1) nitroglycerin patch group (Group 1, n=10), 2) doxazosin mesylate group (Group 2, n=10), 3)combination group with nitroglycerin patch and doxazosin mesylate group (Group3, n=10). After one week of chemical delay, the ear flaps were completely divided and arterialized by anastomosing the central artery to the anterior branch of the central vein. The survival areas of flaps were assessed at 14 days. The mean survival area of each group was 14.7% in the nitroglycerin patch group, 28.5% in the doaxzosin mesylate group and 83.1% in the combination group. The results of this study show that the effect of single pharmacological agent for the viability of an arterialized venous flap is unreliable and the combination of pharmacological agents which act as different mechanisms of flap survival increase the viability much more than the single agent. Also, chemical delay using pharmacological agents has the possibility to substitute for a costly and time-consuming surgical delay procedure.

얼굴·목 아토피피부염에서의 0.1% Tacrolimus 연고 치료의 다기관 관찰연구

류형호 ( Hyeong Ho Ryu ),박영립 ( Young Lip Park ),김광호 ( Kwang Ho Kim ),이종희 ( Jong Hee Lee ),나정임 ( Jung Im Na ),조소연 ( So Yun Cho ) 대한피부과학회 2013 대한피부과학회지 Vol.51 No.11

Background: Atopic dermatitis (AD) is a chronic or chronically relapsing, inflammatory, pruritic skin disease. Because tacrolimus ointment does not induce skin atrophy, it is especially indicated in delicate areas such as the face, intertriginous areas and anogenital region. Objective: With the aim of investigating the differences between the established clinical guidelines and actual treatment response, an open-label, non-comparative, multicenter clinical observational study was undertaken. Methods: One hundred and fifty-three patients with AD in the head and neck area were treated with topical tacrolimus 0.1% twice daily. Clinical evaluations were conducted at baseline, week 4 and week 8. Efficacy was assessed through the investigator`s evaluation of the patients` individual signs (erythema, edema, oozing, excoriation, scaling, lichenification and fissuring) and symptoms (burning, pruritus and telangiectasia) using a 4-point grading system. The physician`s and patient`s global assessments and quality of sleep were also evaluated. Results: Among 153 enrolled patients, 25 (16.3%) were lost to follow-up, and 6 (3.9%) dropped out due to adverse events, leaving 79.7% of the population who completed the trial. Head and neck dermatitis was improved or cleared in 93.6% (week 4) and 94.4% (week 8) by physicians and in 92.1% (week 4) and 90.8% (week 8) by patients. Tacrolimus treatment improved the quality of sleep in head and neck dermatitis patients. Eighty-seven percent of the subjects were compliant, applying it at least 3 days per week. Burning sensation was the most frequently reported adverse event, followed by pruritus and aggravation of the lesion. Conclusion: Tacrolimus ointment 0.1% is a safe and effective treatment option for atopic dermatitis of head and neck including eyelids. (Korean J Dermatol 2013;51(11):851∼857)

모낭의 하부 1/2을 이식시 모낭의 재생

박세정,류형호,서정민,김정철 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

We have examined the regenerative capabilities of the human scalp hair follicle after grafting the lower half of the follicle. Twenty-eight of 32 intact whole-hair follicles isolated from the human scalp regenerated hairs when grafted onto the forehead of the same person. Seven of the 15 lower-half follicles regenerated complete hair follicles 8 months after grafting showed that the lower-half follicle implant reconstituted the complete hair follicle. The sebaceous gland was not regenerated, but there was an outgrowth in the sebaceous gland region. Some grafts formed epithelial cysts. Two years after grafting, the histological examination of the regenerated follicle from the lower-half implant showed that the sebaceous gland was completely regenerated. While an intact follicle shows prominent naked shaft outgrowth, the sheath grows concomitantly with the shaft in lower-half follicles in culture. If grafted lower-half follicles were located too deep, the regrown sheath could not reach the epidermal layer. In this situation, the formation of an epidermal cyst was likely.

원통모양의 유리 전박 피판으로 경부 식도의 재건

백봉수,류형호,이정형,조병채,박준식,변진석 大韓成形外科學會 1996 Archives of Plastic Surgery Vol.23 No.4

Various reconstruction of pharyngoesophageal defects after ablative surgery have been made to restore functions of the pharyngoesophagus. A tubed free radial forearm flap was used to reconstruct the pharyngoesophagus is 23 patients after resection of neoplasms from May 1989 to October 1994. 19 were male and 4 were female. Average patient age 62.2 years. Follow-up period ranged 10 to 64 months with mean 18 months. Oral intake within 3 weeks was possible in 18 patients(78%). 19patients(82%) were discharged within 4 weeks. The immediate postoperative complications were hematoma (n=1), bleeding(n=2), infection(n=3), fistula(m=4), and venous thrombosis(n=1). The late complication was stricture of lower anastomosing site (n=3). The tubed free radial forearm flap had advantages over free jejunal transfer : Larger caliber of the vascular pedicle, longer ischemic time, no laparotomy with less morbidity of the donor site and better toleration to the radiotherapy. Troublesome disadvantages were stricture and fistula formation at suture sites. We modified conventional free radial forearm flap to reduce the complications. 1. A small monitoring flap supplied by the septocutaneous branch of the radial artery was elevated to check the survival of the flap. 2. During tubing, the longitudinal suture site was overlapped with a deepithelialized skin flap and double layer sutures were done to prevent the fistula. 3. Two small triangular flaps were designed and inserted in the distal anastomotic site to prevent circular contracture. The outer layer sutures were anchored to surrounding rigid structure to withstand shrinkage and circular contraction. With this modification, the incidence of stricture and fistula formation was reduced to 13.0% 17.4% respectively and these complication could be treated conservatively.

내시경을 이용한 광배 근판 박리술

이정형,류형호,백봉수,정호윤,조병채 대한미용성형외과학회 1996 Archives of Aesthetic Plastic Surgery Vol.2 No.1

Endoscopic plastic surgery techniques have involved aesthetic procedures such as face lift, breast augmentation, abdominoplasty, and placement of tissue expander. Recently endoscopic harvest of the donor tissue for free flap transfer has been applied in the omentum, jejunum, latissimus dorsi muscle and rectus abdominis muscle. 10 patients who had a soft tissue defect in the foot or in the lower leg were treated with free muscle transfer after endoscopic dissection of the latissimus dorsi muscle from December 1994 to October 1995. 9 patients were male and 1 patient was female. A 5~6cm axillary incision is made, allowing direct identification of the thoracodorsal vascular pedicle. The latissimus dorsi muscle is dissected posteriorly until the limits of open dissection are reached, and then the dissection is continued under endoscopic visualization. The largest harvested muscle was 15×25cm in size. We followed up from 6 to 15 months. We believe that plastic surgeons take advantage of this technique to obtain good and safe results with smaller scrs and reduce postoperative donor site orbidity such as pain and wound healing problems. In conclusion, this procedure may prove particularly useful in women, children, and those who are prone to hypertrophic scars.

가토에서 외과적, 화학적 지연처치가 동맥화된 정맥피판의 생존에 미치는 영향

한상희,류형호,서만수,이무상,조병채,변진석,백봉수 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.1

An arterialized venous flap has the advantages of being thin and pliable, utilizing a large-caliber vein with a pedicle of almost any length, as well as obviating the need to sacrifice a donor artery. However, the main disadvantage of this flap is the partial necrosis of the large flap. The aim of this study was to investigate the efficacy of a surgical delay procedure and a combined surgical and chemical delay procedure on the survival of arterialized venous flaps. Ninety New Zealand white rabbits were divided into three groups: control group, a surgical delay group and a combined surgical and chemical delay group. These groups were further divided into subgroups depending on the delay period and the chemical agents. One arterialized venous flap was made from only one ear of each rabbit due to operative mortality, and 10 rabbits were distributed to each subgroup. The arterialized venous flap had an arterial inflow by anastomosis of the central auricular artery to the anterior branch of the central auricular vein and a venous outflow through the anterior marginal vein. The results were as follows ; 1. Control group : The arterialized venous flaps without any delay procedure showed complete necrosis of all flaps. 2. Surgical delay group : The mean percentages of survival areas of arterialized venous flaps were 36.6% in the 4-day delay group, 59.7% in the 7-day delay group. 3. Combined surgical and chemical delay group: a. A 3-day chemical delay in a continuation of a 4-day simultaneous surgical and chemical delay group: The mean percentages of survival areas of the arterialized venous flaps were 81.1% in the doxazosin mesylate group, 72.8% in the nitroglycerine patch group and 92.9% in a combination group of doxazosin mesylate and nitroglycerine patch. b. A 3-day chemical delay in a continuation of a 7-day simultaneous surgical and chemical delay group : The mean percentages of survival areas of the arterialized venous flaps were 94% in the doxazosin mesylate group, 90.2% in the nitroglycerine patch group and 99% in a combination group of doxazosin mesylate and nitroglycerine patch. In conclusion, the surgical delay procedure increases the percentage of survival areas of the arterialized venous flap in proportion to the delay period. The combination group of surgical and chemical delay procedure had a significant increase of the percentage of survival areas than that of the surgical delay group(p < 0.001). The best survival of the flap was obtained from the subgroup which had a 3-day chemical delay in a continuation of a 7-day simultaneous surgical and chemical delay with combined chemical agents.

형상 기억 합금 고리를 이용한 조갑 감입증의 치료

박현선,류형호,나선재,나세영,박영운,진선필,박세영,이종희,조소연 대한피부과학회 2011 초록집 Vol.49 No.20

Opitz G/BBB Syndrome 1예

변상영,류형호,김보리,박현선,윤현선,조소연 대한피부과학회 2014 大韓皮膚科學會誌 Vol.52 No.9

Opitz G/BBB syndrome (OS)은 배 쪽 정중선 구조의 발달 장애를 특징으로 하는 선천성 다발성 기형 질환이다. OS는 양안 격리증, 광범위한 콧등, 구순구개열을 포함한 얼굴 정중선의 기형과 함께, 후두기관식도기형, 요도하열, 무공항문 등을 특징으로 한다. 또한 중추신경계의 발달장애로 뇌량무발생, 소뇌충부저형성증 등을 나타내거나 심장발달장애로 심방 또는 심실 중격결손증, 동맥관개존증, 대동맥 축착의 특징을 보이기도 하고, OS 환자의 2/3에서는 발달 지연을 동반한다1. 본 증례는 9개월 남자 환아로, 뒤통수 정중앙 두피의 황색 결절과 옆구리 및 허벅지의 선천성 다발성 혈관종 병변을 주증상으로 내원하였다. 혈관종은 각각 1.3 cm×1 cm,1.1 cm×0.9 cm 정도의 크기로, 급격히 자라는 모습은 아니었다. 환아는 특징적으로 얼굴에서 양안격리증, 광범위한 콧등, 그리고 구개구순열을 수술한 흔적을 관찰할 수 있었고, 요도하열, 우측의 내반족 동반하였다(Fig. 1). 양안격리증은 환아의 안쪽 안와간의 간격이 약 3 cm 정도로 정상범위로 판단하는 95% 신뢰구간(1.41 cm∼2.32 cm)을 벗어나는 것으로 진단하였다2. 두피의 병변은 조직검사상선천성 피부결손의 흔적으로 생각되는 국소적 피부 섬유화 소견을 확인할 수 있었다. 또한 환아는 선천심장기형으로 동맥관개존증을 발견하여 수술한 과거력이 있었다. 음식물의 섭취와 배설은 정상적으로 이루어져 위장관의 이상은 발견되지 않았고, 신체검진상 잠복고환, 갈라진 음낭은 동반되지 않았으며, 발달과정 또한 정상으로 뇌질환을 의심할 만한 증거는 없었다(Table 1). 환아는 제태 40주에 미혼모에게 정상 질식분만으로 태어났으며 출생 시 체중이 2.57 kg으로 저체중아에 가까웠고 엄마가 임신 중 담배하루 1갑, 맥주 두 달에 한 캔 마신 과거력이 있었다. 환아는 OS을 의심하여 염색체 검사를 시행하였으나 46, XY 정상소견을 보였고, 가족력의 확인이나 추가적인 유전자 검사 등은 환아가 위탁된 위탁시설의 경제적 문제로 시행이 불가능하였다. 현재까지 보고된 바에 따르면, OS는 다양한 유전양상을 보일 수 있는데, 22q11.2 자리에 위치한 유전자에서의 변이를 보이는 상염색체 우성(autosomal dominant form OS; ADOS)양상3과 Xp22.3 자리에 위한 MID1 유전자의 기능상실로 인한 성염색체 열성(X-linked recessive form OS; XLOS) 양상4이 각각 존재한다. 하지만, 특정 유전자의 이상을 발견할 수 없는 경우나 유전자의 이상이 있더라도 정상 핵형을 보이는 OS 환자도 존재한다. ADOS의 유전자는 현재까지 정확히 확인되지 않았다3. 반면, XLOS를 일으키는 MID1 유전자는 미소관(microtubule)을 구성하여 단백질 포스파타아제 2A (protein phosphatase 2A; PP2A)의 촉매 소단위 분해를 조절하는 역할을 하기 때문에, MID1 유전자 돌연변이는 단백질의 미소관 친화력을 낮추어 OS에서의 정중선 결손의 임상양상을 나타내게 한다5. 한국에서는 조 등6이 2006년 처음으로 4세 남자 환아의 XLOS 증례 1예를 보고한 적이 있었고, 이후 보고된 바는 없다. 본 증례는 OS의 특징적인 임상양상에 추가로 선천성피부결손, 선천성 다발성 혈관종, 내반족이 함께 발견되었던 증례로, 흥미롭게 생각되어 보고하는 바이다.

T-CAM이나 Regenin을 함유한 키토산 스폰지의 창상치유 효과

홍용택,서만수,류형호,박세정,조병채,양정덕,김종엽,정호윤,이동걸,김인산,김상우,배은희,이동신 대한성형외과학회 2004 Archives of Plastic Surgery Vol.31 No.4