http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
김주강,정현정 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1
Recent advances in genome editing technologies based on the CRISPR-Cas system has enabled rapid and accurate alterations of genomic information in various organisms. Functionalization of the genome editing protein Cas9 with various bioactive molecules allows diverse applications of the CRISPR system for both medical and industrial purposes. Herein, we report a versatile approach for introducing chemoselective functionalities into the Cas9 protein via biorthogonal noncannonical amino acid tagging. We used L-azidohomoalanine (AHA) to replace the methionine groups in Cas9, which can be tagged via azide-alkyne cycloaddition for further bioorthogonal functionalization. We characterized the functionalized protein by labeling with fluorophores and visualization. This approach will widen the applications of CRISPR, especially for therapeutic purposes where genetic modification should be performed through a nonviral procedure.
Application of fluorescence-activating and absorption-shifting tag for multiplexed live cell imaging
김주강,정현정 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1
Fluorescent labeling of proteins has been greatly important for bioimaging to study complex molecular mechanisms in cells. The yellow fluorescence-activating and absorption-shifting tag (Y-FAST) is 14-kd protein which by addition of a fluorogen can be rapidly and reversibly activated. However, an effective strategy for multiplexed imaging while allowing temporal control has been lacking. We have developed a multiplexed version of the Y-FAST protein by synthesizing different chromophores that allow fluorescence activation and spectral shifts in various wavelengths. The effects of binding of the fluorogens to Y-FAST were characterized using the purified protein and live cells expressing the protein. This system can be potentially applied for tagging various target proteins for multiplexed imaging in live cells for studies on drug binding, function, and signaling.
Bioorthogonal functionalization of genome editing protein by amino acid tagging and click chemistry
김주강,정현정 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
Recently, CRISPR/Cas9-mediated genome editing system has enabled the rapid and accurate alterations of genomic information in various organisms. The genomic editing protein Cas9 can be functionalized with various bioactive molecules, making it possible to apply a variety of CRISPR systems for both industrial and medical purposes. Herein, we introduce chemoselective functionalities into the Cas9 protein through biorthogonal noncannonical amino acid tagging. We used azidohomoalanine (AHA) to replace the methionine group of Cas9, which can be further labeled by an azide-alkyne cycloaddition reaction with dibenzocyclooctyne. The high reaction rates and its desired biocompatibility allows Cas9 to be functionalized for therapeutic purposes via nonviral procedures. This approach will have great potential for creating bioorthogonally functionalized Cas9 which can widen the application of CRISPR, in particular for the development of therapeutic proteins with new and improved properties.
리튬이온 배터리의 정확한 잔량 측정 기술개발을 위한 연구 방향 제안
주강우,이윤철,하주환,김광선 한국반도체디스플레이기술학회 2016 반도체디스플레이기술학회지 Vol.15 No.2
More efficient use of the battery pack is dependent upon how to measure the remaining capacity of the battery accurately. Among various measurement methods, the basic correction measurement method has still been a hot research topic area to reduce the errors. In this paper, the problems of the existing methods have been investigated and the research direction for measuring more accurate remaining capacity has been suggested by applying the numerical simulations in the future.
주강우,장경민,김광선 한국반도체디스플레이기술학회 2015 반도체디스플레이기술학회지 Vol.14 No.1
This paper is to derive information about the optimal shape of the pack has a minimum temperature range of a Li-ion battery equipped with a module. We selected the shape of the pack in order to reduce the temperature deviation between the batteries as a variable. And we derived the experimental points with a minimum of DOE by D-optimal. We analyzed the temperature and the flow within the battery pack by using a numerical analysis verified in previous studies. We derive the equation for the temperature variation in the objective function using the RSM and performed optimization. As a result, it was confirmed that with the variation in the 1.706e-4 ℃ when to apply an optimized shape.
치료받지 않은 고혈압 환자에서 발생한 신동맥류의 자발성 파열
김지일 ( Kim Ji Il ),서학준 ( Seo Hag Jun ),신옥란 ( Sin Og Lan ),송선화 ( Song Seon Hwa ),황은미 ( Hwang Eun Mi ),주강 ( Ju Gang ),김영수 ( Kim Yeong Su ),윤선애 ( Yun Seon Ae ),김영옥 ( Kim Yeong Og ) 대한신장학회 2003 Kidney Research and Clinical Practice Vol.22 No.6
Spontaneous rupture of renal artery aneurysm is a rare, but life threatening complication with high mortality. The etiology of non-traumatic renal artery aneurysm is fibromuscular dysplasia, atherosclerosis, vasculitis, and pregnancy. We here report a case of spontaneous rupture of renal artery aneurysm in a patient with untreated hypertension. A 39-year-old non-pregnant woman complained of sudden onset of right flank and lower quadrant abdominal pain. Ul-trasonography showed large fluid collection in right lower abdomen. Emergency laparotomy demonstrated huge retroperitoneal hematoma due to spontaneous rupture of right renal artery aneurysm. Microscopic examination of the aneurysmal wall revealed intimal hyperplasia without atherosclerotic change. (Korean J Nephrol 2003;22(6):763-766)
김광선,김준영,장경민,주강우 한국반도체디스플레이기술학회 2013 반도체디스플레이기술학회지 Vol.12 No.4
In the semiconductor heat-treatment process, the temperature uniformity determines the film quality of a wafer. This film quality effects on the overall yield rate. The heat transfer of the wafer surface in the heat-treatment process equipment is occurred by convection and radiation complexly. Because of this, there is the nonlinearity between the wafer temperature and reactor. Therefore, the accurate prediction of temperature on the wafer surface is difficult without the direct measurement. The thermal camera and the T/C wafer are general ways to confirm the temperature uniformity on the heat-treatment process. As above ways have limit to measure the temperature in the precise domain under the micro-scale. In this study, we developed the thin film type temperature sensor using the MEMS technology to establish the system which can measure the temperature under the micro-scale. We combined the experiment and numerical analysis to verify and calibrate the system. Finally, we measured the temperature on the wafer surface on the semiconductor process using the developed system, and confirmed the temperature variation by comparison with the commercial T/C wafer.