Membrane Microarray를 이용한 Resveratrol에 의해 차별적으로 발현되는 유전자 군의 분석

김종식,장민정,김효은,김순영,김병오,손호용,Kim, Jong-Sik,Jang, Min-Jung,Kim, Hyo-Eun,Kim, Soon-Young,Kim, Byung-Oh,Sohn, Ho-Yong 한국생명과학회 2007 생명과학회지 Vol.17 No.8

본 연구에서는 다섯 종류의 phytochemical (resveratrol, genistein, epicatechin gallate, diaIly disulfide, caffeic acid phenetyl ester)과 sulindac sulfide가 암 억제 단백질 p53을 유도할 수 있는지에 대해 연구하였다. 처리한 모든 phytochemical에 의해 p53 단백질의 발현이 강하게 유도된 반면, sulindac sulfide에 의해서는 p53 단백질이 유도되지 않았다. 처리한 phytochemical 중 포도껍질이나 와인에 많이 들어있는 resveratrol에 의해 p53 단백질이 농도의존적 혹은 처리시간 의존적으로 증가 발현되는 것을 확인하였다. 암 억제 단백질인 p53 하위 단계의 유전자들만 집적되어 있는 membrane microarray를 이용하여 실험을 수행한 결과, 25개의 유전자가 up-regulation 된 반면, 2개의 유전자가 down-regulation 되는 것을 확인하였다. Up-regulation 되는 유전자중 4개를 선택하여, RT-PCR을 수행한 결과 모두 membrane microarray 실험의 결과와 일치하였다. 게다가 p53 null인 HCT116 세포주를 이용한 RT-PCR을 통하여 TSP-1 유전자의 발현은 p53 의존적이지 않은 반면, MASPIN 유전자는 p53 의존적임을 확인하였다. 이러한 연구 결과는 resveratrol에 의한 화학적 암 예방법의 분자생물학적 기전을 이해하는데 도움을 줄 것으로 기대된다. In the present study, we investigated whether several phytochemicals (resveratrol, genistein, epicatechin gallate, dially disulfide, caffeic acid phenetyl ester) and sulindac sulfide could induce expression of tumor suppressor p53 protein in human colorectal HCT116 cells. We found that p53 was dramatically induced by all phytochemical treatments except sulindac sulfide. Among treated phytochemicals, we selected resveratrol for further experiments because it is one of the highest p53 inducer. Using a Western blot analysis, we found that resveratrol induced p53 in a dose- and time-dependent manner. Additionally, using membrane-based microarray analysis, we found that twenty-five genes were up-regulated and two genes were down-regulated by resveratrol treatment. Among the up-regulated genes, we selected 4 genes and performed reverse-transcription-PCR to confirm microarray data. The results of RT-PCR were highly accorded with those of membrane microarray. In addition, we found that thrombospondin-1 (TSP-1) expression was not dependent on p53 presence, whereas mammary serine protease inhibitor (MASPIN) expression was dependent on p53 expressed by resveratrol treatment. The results of this study may help to promote our understandings of the molecular mechanisms of chemoprevention that are mediated by resveratrol in human colorectal cancer.

메타프로테오믹스의 미생물생태학적 응용

김종식,우정희,김준태,박년호,김충곤,Kim, Jong-Shik,Woo, Jung-Hee,Kim, Jun-Tae,Park, Nyun-Ho,Kim, Choong-Gon 한국미생물학회 2010 미생물학회지 Vol.46 No.1

미생물 군집과 기능연구를 위한 최근의 새로운 분석기술의 발전은 다양한 유전 관련 정보를 제공해왔다. mRNA를 포함하는 핵산을 기초로 한 연구를 뛰어넘어서 메타프로테오믹스는 미생물 군집의 유전형 및 표현형의 특징적인 정보를 보다 정교하게 제공할 수 있다. 이미 서로 다른 미생물 생태계인 해수, 인간의 배설물, 활성 슬러지, 산성 광산 폐수 생물막, 토양 등에 메타프로테오믹스 기술이 유용하게 사용되었다. 이들 연구는 여러 측면에서 상당히 다르지만 미생물 군집의 구조, 기능, 생리, 상호관계, 생태, 진화적 측면을 결정적으로 상호 연결한다는 것을 밝혀냈다. 본 총설은 메타프로테오믹스에 대한 현재까지의 가장 최신의 정보를 요약하여 제공함으로써 메타프로테오믹스에 대한 정확한 이해와 활용을 통해 다방면의 메타프로테오믹스가 가능하도록 하고자 하였다. New technologies are providing unprecedented knowledge into microbial community structure and functions. Even though nucleic acid based approaches provide a lot of information, metaproteomics could provide a high-resolution representation of genotypic and phenotypic traits of distinct microbial communities. Analyzing the metagenome from different microbial ecosystems, metaproteomics has been applied to seawater, human guts, activated sludge, acid mine drainage biofilm, and soil. Although these studies employed different approaches, they elucidated that metaproteomics could provide a link among microbial community structure, function, physiology, interaction, ecology, and evolution. These approaches are reviewed here to help gain insights into the function of microbial community in ecosystems.

간헐성 복통과 구토를 주소로 하는 중장염전이 동반된 장 이상회전 1례

김종식,정주영,박동철,김상우,김홍주,김영훈,Kim, Jong-Shik,Chung, Ju-Young,Park, Dong-Churl,Kim, Sang-Woo,Kim, Hong-Joo,Kim, Young-Hoon 대한소아소화기영양학회 2002 Pediatric gastroenterology, hepatology & nutrition Vol.5 No.1

저자들은 간헐적인 구토와 복통을 주소로 내원한 6세 된 남아에서 상부 위장관 조영술과 복부단층촬영으로 진단된 중장염전을 동반한 장 이상회전 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Intestinal malrotation is a developmental anomaly resulting from embryologic failure of fixation and rotation of the gut and predisposed to midgut volvulus and small bowel obstruction. Acute midgut volvulus is most often encountered in the newborn period. But older children and adults may have a history of intermittent episodes of partial volvulus presenting with recurrent colicky abdominal pain accompanying bilious vomiting. We experienced a case of intestinal malrotation complicated by midgut volvulus presented with recurrent vomiting and abdominal pain in a 6-year-old boy.

흰쥐에서 대사작용 억제에 의한 혈중 Mg²⁺ 조절

김종식,김상진,김진상,Kim, Jong-shick,Kim, Shang-jin,Kim, Jin-shang 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.1

Magnesium ($Mg^{2+}$) plays an important role in the regulation of a range of intracellular processes. Regulation of extracellular $Mg^{2+}$ contents was studied in the anesthetized Sprague-Dawley (SD) rats. Animals were injected intraperitoneally with sodium nitrite ($NaNO_2$), and circulating $Mg^{2+}$($[Mg^{2+}]c$) was measured after the injection and then 10 and 20 minutes later. A dose-dependent increase in $[Mg^{2+}]c$ was observed in animals injected with $NaNO_2$ at a dose of 10mg/kg or higher. Pretreatment with methylene blue prevented the $NaNO_2$-induced increase in $[Mg^{2+}]c$. $[Mg^{2+}]c$ displayed an inverse linear correlation with hemoglobin and exponential correlation during $NaNO_2$ injection. Injection of KCN or rotenone also induced an increase in $[Mg^{2+}]c$. An increase in $[Mg^{2+}]c$ was observed when respiration rate was reduced from 100/min (140ml/min) to 10/min (14ml/min) during 30 min. These results indicate that changes in $[Mg^{2+}]c$ inversely reflect alteration of ATP in a model of metabolic inhibition.

탐방-뉴질랜드 루트번트랙에서

김종식,Kim, Jong-Sik 한국자연공원협회 1997 한국자연공원 Vol.66 No.-

주정원료 및 제조방법과 그 특성

김종식,Kim, Jong-Sik 한국주류산업협회 1992 주류산업 Vol.12 No.1

'91중국국제과학기술학술회의 참가기

김종식,Kim, Jong-Sik 한국과학기술단체총연합회 1991 과학과 기술 Vol.24 No.11

Correction of Dose Distribution at Total Body Irradiation using Compensator

김종식,조현상,김영곤,조정근,주상규,박영환,Kim Jong Sik,Cho Hyun Sang,Kim Young Kon,Cho Jung Keun,Ju Sang Kyu,Park Young Hwan 대한방사선치료학회 1997 大韓放射線治療技術學會誌 Vol.9 No.1

The using of compensator is required to adjust the irregular dose distribution due to irregular thickness of the body in Total Body Irradiation. Aluminuim, copper or lead is generally used as compensator. In our study, we would like to introduce a result of the attenuation and compensation effect of radiation use compensator made by duralumin and its clinical use. The thickness of compensator was calculated by the attenustion of radiation, which was measured by polystyrene phantom and ionization chamber(farmer). The compensation effect of radiation was measured by diode detector. All of conditions were set as in real treatment, and the distanc from source to detector was 446 cm. We also made fixation of device to easily attach the compensator to LINAC. Beam spoiler was menufactured and placed on the patient to irradiate sufficient dose to the skin. diode detector were placed on head, neck, chest, umbilicus. pelvis and knee with each their entranced exit points, and datas of dose distribution were evaluated and compared in each points for eleven patients(Feb. 96-Feb. 97). The attenuation rate of irradiation by duralumin compensator was measured as $1.4\%$ in 2mm thickness. The mean attenuation rate was $1.3\%$ per 2mm as increasing the thickness gradually to 50 mm. By using duralunim compensator, dose distribution in each points of body was measured with ${\pm}2.8\%$ by diode detectior. We could easily calculate the thickness of compensator by measuring the attenuation rate of radiation, remarkably reduce the irragularity of dose distribution duo to the thickness of body and magnify the effect of radiation therapy.

난분해성물질을 함유한 매립장 침출수의 화학적 처리에 관한 연구

김종식,최준호 ( Jong Shik Kim,Joon Ho Choi ) 한국공업화학회 1997 한국공업화학회 연구논문 초록집 Vol.1997 No.0

N/A Treatment of landfill leachate using ozonation and coagulation process were investigated. Ferric(III) chloride and Cation were found to be effective coagulants to treat landfill leachate. In the case of ozonation, leachate qualities were improved as pH increasing. Preozonation process removed more CODcr, Color(535nm) and Turbidity than Post-ozonation about 8%, 3.5% and 1% respectively.

시론-수학자가 이룬 노력의 산물 잊지말자

김종식,Kim, Jong-Sik 한국과학기술단체총연합회 1989 과학과 기술 Vol.22 No.7