알레르기 비염의 한의 진료 현황 설문조사

김영은,정의민,이동효,Kim, Young-Eun,Jeong, Ui-Min,Lee, Dong-Hyo 대한한방안이비인후피부과학회 2017 한방안이비인후피부과학회지 Vol.30 No.4

Objectives : The purpose of this study was to investigate the treatment trend for allergic rhinitis in Korean Medicine. Methods : We conducted an online survey for Korean Medicine Doctors who were registered in the association of Korean Medicine. The questionnaire was consisted of patient characteristics, diagnosis status, treatment status, and future research needs. Results : Data from total of 396 respondents were analyzed. More than 70% of the patient came to the Korean Medicine Clinic after visiting the Western Medicine Clinic in 43.9% of the respondents. 55.6% of the respondents performed combination therapy. History taking, nasal examination, x-ray, and Korean Medicine diagnostic test were used for diagnosis. The mean duration of treatment ranged from $4.9{\pm}2.91$ to $15.2{\pm}8.45$ for pediatric and early childhood patients and from $17.5{\pm}16.15$ to $5.3{\pm}3.85$ weeks for adolescents and adult patients. The mean number of treatment times was from $9.8{\pm}7.00$ to $33.5{\pm}24.45$ for pediatric and early childhood patients and from $10.8{\pm}11.55$ to $40.4{\pm}48.18$ times for adolescents and adult patients. 64.5%, 48.0%, and 91.2% of the respondents used herbal medication in national health insurance coverage, herbal medication uninsured in health insurance and herbal prescription filled at each medical institution, respectively for pediatric and early childhood patients and 67.6%, 42.8% and 86.1% for adolescents and adult patients. 36.9% and 36.4% of respondents answered that the study of acupuncture and herbal medicine are needed preferentially. Conclusions : The results of this survey will be used to develop clinical practice guideline that reflect actual clinical practice.

녹용의 약효 성분에 관한 연구 ( Ⅴ ) 녹용 및 판토크린의 당지질과 인지질의 조성에 관하여

김영은,임동구,신승언 ( Young Eun Kim,Dong Koo Lim,Seung Uon Shin ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.3

The lipid soluble fraction of Antler velvet layer (cervus nippon taiouanous) was extracted and compared to that of pantocrin (ethanol preparation of Antler, commercially available). Lipid soluble components (801㎎/24.5g from Antler velvet ayer and 979.1㎎/143㎖ from pantocrin) were fractionated by gel filteration using a sephadex CT-25 into neutral lipids, sulfatides, cerebrosides, phospholipids and non-lipid components (lipoprotein and peptide). A silicic acid and a DEAE-sephadex A-25 column chromatography were followed from refractionation and purification. Each fraction was identified by thin layer chromatography using standard materials. Typical lipid soluble fraction of Antler contained 65.3% neutral lipid, 5.2% glycolipid, 12.9% phospholipid, 6.1% ganglioside and 2.9% non-lipid components. The glycolipid was composed of 63.5% cerebroside and 36.5% sulfatide. The cerebroside fraction of the Antler velvet layer showed four spots while pantocrin gave only one spot on a thin layer chromatogram. Among the four spots of Antler cerebroside two had almost same migration with standard but two had quite different mobility compared to the standard. The latter fractions were hydrolyzed by 0.025M methanolic HCl in order to desulfate. The two unknown materials were identified as sulfatides by the method of IR spectroscopy and thin layer chromatography. By two dimensional thin layer chroma tography, it was found that phospholipid of thr. Antler velvet layer were composed of phosphatidylethanolamine, sphingomyelin, phosphatidylcholine, lysophospahtidylethanolamine, and lysophosphatidylcholine and phosphatidic acid. On the other hand pantocrin contained phosphatidylcholine and sphingomyelin as phospholipids.

녹용의 약효 성분에 관한 연구 ( Ⅳ ) 녹용 벨벳층의 프로스타그란딘의 검출

김영은,이승기,이명희 ( Young Eun Kim,Seung Ki Lee,Myoung Hee Lee ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.1

The fresh antler was cut, frozen and mechanically separated into spongy bone layer and velvet layer. The prostaglandin-like components were extracted from antler velvet layer and qualitatively analyzed by three different methods: thin layer chromatography, ultra-violet spectroscopy after conversion to PGB series by alkaline treatment, and gas liquid chromatography. The prostaglandins detected were PGE₂, 15-epi-PGE₁, PGF_(1α), PGF_(1β). The experiments indicated that the petroleum ether fraction contained mostly PGE series and that the ethylether fraction contained PGF series. One major prostaglandin-like components of antler velvet layer was found to be the main compound in the petroleum ether extract. The structure of this compound is still unknown but the gas liquid chromatography data suggest that it is a hydroxy fatty acid.

기혈양허로 변증한 파킨슨병 환자 증례 보고

김영은,김일화,이재화,이성근,이기상,Kim, Young-Eun,Kim, Il-Wha,Lee, Jae-Hwa,Lee, Seoung-Geun,Lee, Key-Sang 대한한방내과학회 2009 大韓韓方內科學會誌 Vol.30 No.4

Parkinson's disease is a slowly progressive degenerative disorder of the central nervous system. It is characterized by tremor when muscles are at rest, increased muscle tone, slowness of voluntary movements, and difficulty maintaining balance. In oriental medicine, these symptoms are diagnosed as yin(陰)-deficiency of liver and kidney, deficiency of qi(氣) and blood, retention of phlegm(痰), qi-stagnation and blood stasis. In this case, we diagnosed patients as deficiency of qi(氣) and blood type according to symptoms and treated by herbs that strengthen yang and benefiting yin for two weeks, while maintaining existing parkinson's western medication. After treatment, clinical symptoms were improved, while UPDRS (Unified Parkinson's Disease Rating Scale) score was decreased. These cases suggest that oriental medicine therapy maybe effective in the treatment of Parkinson's disease.

RF 마그네트론 스퍼터 방법에 의한 다결정 NiO 박막의 비저항 변화

김영은,노영수,박동희,최지원,채근화,김태환,최원국,Kim, Youmg-Eun,No, Young-Soo,Park, Dong-Hee,Choi, Ji-Won,Chae, Keun-Hwa,Kim, Tae-Hwan,Choi, Won-Kook 한국진공학회 2010 Applied Science and Convergence Technology Vol.19 No.6

NiO 산화물 타겟을 이용한 RF 마그네트론 스퍼터 방법으로 유리 기판 위에 NiO 박막을 Ar 가스만을 사용하여 증착하였으며, 증착 온도에 따라 NiO 박막 특성에 미치는 영향을 조사하였다. XRD 측정으로부터 증착된 박막의 결정구조는 $200^{\circ}C$ 이하에서 (111) 면의 우선 배향성으로 보이다가 $350^{\circ}C$ 이상에서 (220) 면의 우선 배향성을 가지는 다결정 입방구조임을 확인하였다. NiO 박막의 전기적 특성의 변화는 기판의 온도가 $200^{\circ}C$까지는 $10^5\;{\Omega}cm$의 부도체에 가까운 높은 비저항을 보였고 기판의 온도가 $300^{\circ}C$ 이상에서는 $10^{-1}{\sim}10^{-2}{\Omega}cm$의 도체의 특성을 보이는 낮은 비저항으로 감소하는 Mott-Insulator Transition(MIT) 현상을 관측하였다. NiO 박막 내의 증착 온도 변화에 따른 ${\sim}10^7$ 정도의 큰 비저항 변화를 결정성, 결정립의 변화 및 밴드 갭의 변화 등으로 설명하였다. Polycrystalline NiO thin films were deposited on glass substrate by RF magnetron sputtering using only Ar as a plasma sputter gas. based on the analysis of x-ray diffraction (XRD), NiO films had a polycrystalline cubic (NaCl type) structure. NiO thin films grown below and above $200^{\circ}C$ showed preferred orientation of (111) and (220) respectively. It showed colossal change in electrical resistivity as much a ${\sim}10^7$ order form an insulating state of $105\;{\Omega}cm$ below $200^{\circ}C$ to a conducting state of $10^{-2}{\sim}10^{-1}\;{\Omega}cm$ above $300^{\circ}C$ such a Mott metal-insulator transition (MIT) in polycrystalline.

전방 충돌에 따른 유아 승객 거동을 위한 3세 유아 모델의 개발

김영은,김희석,Kim, Yeong-Eun,Kim, Hui-Seok 대한의용생체공학회 1999 의공학회지 Vol.20 No.1

최근 들어 많은 관심에 대상이 되고 있는 동차 사고시 어린이 승객의 부상현상을 해석하기 위하여 범용 동력학 패키지인 DADS를 이용하여 3세 어린이 모델을 개발하였다. 모델은 모두 14개의 요소들과 12개의 조인트로 구성 시켰으며 부스터를 장착한 시트에 3점식 벨트를 체결한 형태로 개발되었으며 썰매를 이용한 전방 충돌 시뮬레이션 결과 기존의 더미를 이용한 실험 결과와 일치하는 응답 특성을 얻을 수 있었다. In order to increase our understanding of the injury mechanism in the child occupant, three year old child model was developed using commercial dynamic package DADS. Total 14 segments and 12 joints were used to compose a model in three points belted condition with booster seat. HYGE sled test case was simulated to validate the developed model. Based on the comparison of the model and published test results, the developed model appears to be a resonable representation of the three year old dummy.

녹용의 약효 성분에 관한 연구(V) 녹용 및 판토크린의 당지질과 인지질의 조성에 관하여

김영은,임동구,신승언,Kim, Young-Eun,Lim, Dong-Koo,Shin, Seung-Uon 생화학분자생물학회 1977 한국생화학회지 Vol.10 No.3

녹용의 지용성 성분에 관한 생화학적 연구의 일환으로 대만산 꽃사슴(Cervus nippon taiouans)의 녹용을 직접 절단하여 동결시킨 후, 벨?殼?(velvet layer)과 해면상 골조직층(spongybone layer)을 분리하였다. 조직을 Virtis homogenizer로 분쇄한 후 $CHCl_3$ : $CH_3OH$ (1 : 2) 및 (2 : 1) 비율의 혼합 용매로 지용성 성분을 추출하고, Rouser 법에 따라서 Sephadex G-25 column chromatography를 행하여 총지질로부터 ganglioside 및 nonlipid를 분리하고 이어서 Rouser의 방법에 준하여 silicic acid column chromatography를 행하여 총지질을 중성지질, 당지질, 인지질로 분리하였다. 대조시험으로, 녹용의 수성에탄올 제제인 Pantocrin을 질소 기류하에서 감압 건조한후, 상기와 동일한 방법으로 지질 성분을 분획하였다. 지용성 성분의 건조 중량에 대한 각 분획의 백분율은 녹용의 경우 neutrallipid 65.30%, glycolipid 5.22%, phospholipid 12.86%, ganglioside 6.12%, nonlipid(proteolipid & peptide) 10.51%의 비율로 합류되어 있었으며, Pantocrin은 neutral lipid 41.7%, glycolipid 0.62%, phospholipid 5.39%, ganglioside 49.32%, nonlipid(proteolipid & peptide) 2.93%의 함량이었다. 녹용의 glycolipid는 DEAE Sephadex A-25 column chromatography를 행하여 glycolipid의 전체양에 대하여 cerebroside 63.5%, sulfatide 36.5%를 얻었으며 각각을 표준품과 비교하여 T.L.C.를 행하여 7종의 spots를 얻었으며 그중 2종은 cerebroside로 3종은 sulfatide로 확인되었으나 2종은 미지물질로 확인되지 않았다. Pantocrin에서는 1종의 cerebroside를 확인하였다. 녹용과 Pantocrin에 존재하는 ganglioside는 Sephadex G-25 column chromatography법보다는 용매 분획법에 의해 얻은 시료에서 T.L.C.법으로 확인하였다, 또 녹용에서 얻은 phospholipid로는 phosphatidyl ethanolamine, phosphatidyl choline, sphingomyelin, phosphatidic acid, lysophosphatidyl ethanolamine 및 lysophosphatidyl choline등으로 동정되었으며, Pantocrin에서는 phosphatidyl choline, sphingomyelin을 검출하였다. The lipid soluble fraction of Antler velvet layer (cervus nippon taiouanous) was extracted and compared to that of pantocrin (ethanol preparation of Antler, commercially available). Lipid soluble components (801mg/24.5g from Antler velvet ayer and 979.1mg/143ml from pantocrin) were fractionated by gel filteration using a sephadex CT-25 into neutral lipids, sulfatides, cerebrosides, phospholipids and non-lipid components (lipoprotein and peptide). A silicic acid and a DEAE-sephadex A-25 column chromatography were followed from refractionation and purification. Each fraction was identified by thin layer chromatography using standard materials. Typical lipid soluble fraction of Antler contained 65.3% neutral lipid, 5.2% glycolipid, 12.9% phospholipid, 6.1% ganglioside and 2.9% non-lipid components. The glycolipid was composed of 63.5% cerebroside and 36.5% sulfatide. The cerebroside fraction of the Antler velvet layer showed four spots while pantocrin gave only one spot on a thin layer chromatogram. Among the four spots of Antler cerebroside two had almost same migration with standard but two had quite different mobility compared to the standard. The latter fractions were hydrolyzed by 0.025M methanolic HCl in order to desulfate. The two unknown materials were identified as sulfatides by the method of IR spectroscopy and thin layer chromatography. By two dimensional thin layer chroma tography, it was found that phospholipid of the Antler velvet layer were composed of phosphatidylethanolamine, sphingomyelin, phosphatidylcholine, lysophospahtidylethanolamine, and lysophosphatidylcholine and phosphatidic acid. On the other hand pantocrin Ccontained phosphatidylcholine and sphingomyelin as phospholipids.

성인 뇌와 유아 뇌 모델의 진동 특성 해석

김영은,유진환,Kim, Yeong-Eun,Yu, Jin-Hwan 대한의용생체공학회 1998 의공학회지 Vol.19 No.6

머리(뇌 포함) 부분에 대한 3차원 유한 요소 모델을 이용하여 진동 방향에 따른 응답특성을 해석하였다. 해석 결과 성인의 뇌는 9Hz, 14Hz에서 고유 진동수를 나타내었으며 유아의 뇌에서는 2Hz, 3Hz에서 고유 진동수가 계산되었다. 진동 방향에 관계없이 각 진동에 대해 계산된 가속도를 제외한 전단력, 압력, von-Mises 값의 최대, 최소값의 비는 성인에 비해 유아모델에서 더 크게 나타났다. 각 고유 진동수에서 성인모델은 14Hz에서 상지 중추보다 하지 중추를 관장하는 뇌의 영역에서 보다 큰 응력이 계산되어 상지에 의한 오동작 보다 하지에 의한 오동작을 일으킬 가능성이 더 큼을 유추할 수 있었다. Using three dimensional finite element model of the human brain, vibratory characteristics of the human brain according to vibratory direction was analyzed. From this analysis 9, 14Hz and 2, 3Hz natural frequencies were calculated for adult's and baby's brain model respectively. Regardless of the vibratory direction relatively high shear stress, pressure and von Mises stress variation except acceleration were detected in the baby brain model. At each natural frequencies, adult's model showed relatively high stress level in the region of lower limb control area compared with upper limb control area at 14Hz natural frequency.

단어 단위 접근법을 이용한 음운장애 아동과 정상 아동의 음운 분석

김영은,최성일,박상희,Kim, Young-Eun,Choi, Sung-Il,Park, Sang-Hee 한국음성학회 2006 음성과학 Vol.13 No.4

Recently, many researchers have been interested in children with phonological disorders. The purpose of this study was to examine those children in comparison with normal children and to find better assessment criteria of the whole-word approach. Three children with phonological disorders and three normal children of 5 to 7 years old participated in the picture description tasks. Results of this study were as follows: there was a significant difference in the whole-word assessment between normal and phonological disorder children. Such criteria as whole-word correctness, whole-word complexity, whole-word intelligibility proved to be good for diagnosing children's phonological disorders. Further studies would be desirable to apply the approach to more children of various age groups.

표준 요 시료 중 Oxalate의 측정을 위한 FT-NIR 분광기의 유용성 검정

김영은,홍수형,김정완,이종영,Kim, Yeong-Eun,Hong, Su-Hyung,Kim, Jung-Wan,Lee, Jong-Young 대한예방의학회 2006 예방의학회지 Vol.39 No.2

Objectives : The determination of oxalate in urine is required for the diagnosis and treatment of primary hyperoxaluria, idiopathic stone disease and various intestinal diseases. We examined the possibility of using Fourier transform near-infrared (FT-NIR) spectroscopy analysis to quantitate urinary oxalate. The practical advantages of this method include ease of the sample preparation and operation technique, the absence of sample pre-treatments, rapid determination and noninvasiveness. Methods : The range of oxalate concentration in standard urine solutions was $0-221mg/{\ell}$. These 80 different samples were scanned in the region of 780-1,300 nm with a 0.5 nm data interval by a Spectrum One NTS FT-NIR spectrometer. PCR, PLSR and MLR regression models were used to calculate and evaluate the calibration equation. Results : The PCR and PLSR calibration models were obtained from the spectral data and they are exactly same. The standard error of estimation (SEE) and the % variance were $10.34mg/{\ell}$ and 97.86%, respectively. After full cross validation of this model, the standard error of estimation was $5,287mg/{\ell}$, which was much smaller than that of the pre-validation. Furthermore, the MCC (multiple correlation coefficient) was 0.998, which was compatible with the 0.923 or 0.999 obtained from the previous enzymatic methods. Conclusions : These results showed that FT-NIR spectroscopy can be used for rapid determination of the concentration of oxalate in human urine samples.