Genetically engineered antibody fusion proteins : potential drug delivery to the brain and their applications

신승언 경희대학교 동서약학연구소 1998 제4회 동서약학을 통한 신약개발 심포지움 Vol.- No.-

녹용중 지용성 성분의 당대사에 미치는 영향 Aldolase 의 활성화에 대하여

김영은,신승언 생화학분자생물학회 1984 BMB Reports Vol.10 No.4

음향방출신호를 이용한 밸브의 내부 누설 진단

이정형,신승언,서재영,신호연 해양환경안전학회 2021 해양환경안전학회 학술발표대회 논문집 Vol.2021 No.11

녹용의 약효 성분에 관한 연구 ( Ⅴ ) 녹용 및 판토크린의 당지질과 인지질의 조성에 관하여

김영은,임동구,신승언 ( Young Eun Kim,Dong Koo Lim,Seung Uon Shin ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.3

The lipid soluble fraction of Antler velvet layer (cervus nippon taiouanous) was extracted and compared to that of pantocrin (ethanol preparation of Antler, commercially available). Lipid soluble components (801㎎/24.5g from Antler velvet ayer and 979.1㎎/143㎖ from pantocrin) were fractionated by gel filteration using a sephadex CT-25 into neutral lipids, sulfatides, cerebrosides, phospholipids and non-lipid components (lipoprotein and peptide). A silicic acid and a DEAE-sephadex A-25 column chromatography were followed from refractionation and purification. Each fraction was identified by thin layer chromatography using standard materials. Typical lipid soluble fraction of Antler contained 65.3% neutral lipid, 5.2% glycolipid, 12.9% phospholipid, 6.1% ganglioside and 2.9% non-lipid components. The glycolipid was composed of 63.5% cerebroside and 36.5% sulfatide. The cerebroside fraction of the Antler velvet layer showed four spots while pantocrin gave only one spot on a thin layer chromatogram. Among the four spots of Antler cerebroside two had almost same migration with standard but two had quite different mobility compared to the standard. The latter fractions were hydrolyzed by 0.025M methanolic HCl in order to desulfate. The two unknown materials were identified as sulfatides by the method of IR spectroscopy and thin layer chromatography. By two dimensional thin layer chroma tography, it was found that phospholipid of thr. Antler velvet layer were composed of phosphatidylethanolamine, sphingomyelin, phosphatidylcholine, lysophospahtidylethanolamine, and lysophosphatidylcholine and phosphatidic acid. On the other hand pantocrin contained phosphatidylcholine and sphingomyelin as phospholipids.

녹용의 약효 성분에 관한 연구(V) 녹용 및 판토크린의 당지질과 인지질의 조성에 관하여

김영은,임동구,신승언,Kim, Young-Eun,Lim, Dong-Koo,Shin, Seung-Uon 생화학분자생물학회 1977 한국생화학회지 Vol.10 No.3

녹용의 지용성 성분에 관한 생화학적 연구의 일환으로 대만산 꽃사슴(Cervus nippon taiouans)의 녹용을 직접 절단하여 동결시킨 후, 벨?殼?(velvet layer)과 해면상 골조직층(spongybone layer)을 분리하였다. 조직을 Virtis homogenizer로 분쇄한 후 $CHCl_3$ : $CH_3OH$ (1 : 2) 및 (2 : 1) 비율의 혼합 용매로 지용성 성분을 추출하고, Rouser 법에 따라서 Sephadex G-25 column chromatography를 행하여 총지질로부터 ganglioside 및 nonlipid를 분리하고 이어서 Rouser의 방법에 준하여 silicic acid column chromatography를 행하여 총지질을 중성지질, 당지질, 인지질로 분리하였다. 대조시험으로, 녹용의 수성에탄올 제제인 Pantocrin을 질소 기류하에서 감압 건조한후, 상기와 동일한 방법으로 지질 성분을 분획하였다. 지용성 성분의 건조 중량에 대한 각 분획의 백분율은 녹용의 경우 neutrallipid 65.30%, glycolipid 5.22%, phospholipid 12.86%, ganglioside 6.12%, nonlipid(proteolipid & peptide) 10.51%의 비율로 합류되어 있었으며, Pantocrin은 neutral lipid 41.7%, glycolipid 0.62%, phospholipid 5.39%, ganglioside 49.32%, nonlipid(proteolipid & peptide) 2.93%의 함량이었다. 녹용의 glycolipid는 DEAE Sephadex A-25 column chromatography를 행하여 glycolipid의 전체양에 대하여 cerebroside 63.5%, sulfatide 36.5%를 얻었으며 각각을 표준품과 비교하여 T.L.C.를 행하여 7종의 spots를 얻었으며 그중 2종은 cerebroside로 3종은 sulfatide로 확인되었으나 2종은 미지물질로 확인되지 않았다. Pantocrin에서는 1종의 cerebroside를 확인하였다. 녹용과 Pantocrin에 존재하는 ganglioside는 Sephadex G-25 column chromatography법보다는 용매 분획법에 의해 얻은 시료에서 T.L.C.법으로 확인하였다, 또 녹용에서 얻은 phospholipid로는 phosphatidyl ethanolamine, phosphatidyl choline, sphingomyelin, phosphatidic acid, lysophosphatidyl ethanolamine 및 lysophosphatidyl choline등으로 동정되었으며, Pantocrin에서는 phosphatidyl choline, sphingomyelin을 검출하였다. The lipid soluble fraction of Antler velvet layer (cervus nippon taiouanous) was extracted and compared to that of pantocrin (ethanol preparation of Antler, commercially available). Lipid soluble components (801mg/24.5g from Antler velvet ayer and 979.1mg/143ml from pantocrin) were fractionated by gel filteration using a sephadex CT-25 into neutral lipids, sulfatides, cerebrosides, phospholipids and non-lipid components (lipoprotein and peptide). A silicic acid and a DEAE-sephadex A-25 column chromatography were followed from refractionation and purification. Each fraction was identified by thin layer chromatography using standard materials. Typical lipid soluble fraction of Antler contained 65.3% neutral lipid, 5.2% glycolipid, 12.9% phospholipid, 6.1% ganglioside and 2.9% non-lipid components. The glycolipid was composed of 63.5% cerebroside and 36.5% sulfatide. The cerebroside fraction of the Antler velvet layer showed four spots while pantocrin gave only one spot on a thin layer chromatogram. Among the four spots of Antler cerebroside two had almost same migration with standard but two had quite different mobility compared to the standard. The latter fractions were hydrolyzed by 0.025M methanolic HCl in order to desulfate. The two unknown materials were identified as sulfatides by the method of IR spectroscopy and thin layer chromatography. By two dimensional thin layer chroma tography, it was found that phospholipid of the Antler velvet layer were composed of phosphatidylethanolamine, sphingomyelin, phosphatidylcholine, lysophospahtidylethanolamine, and lysophosphatidylcholine and phosphatidic acid. On the other hand pantocrin Ccontained phosphatidylcholine and sphingomyelin as phospholipids.

인체 LDL에서 분리한 Apo B-100의 표면 및 중심부위에서 지질과 결합하는 peptide에 관한 연구

조현미,정희경,황기철,장양수,신승언,김태웅 한국지질동맥경화학회 2001 韓國脂質學會誌 Vol.11 No.4

모터구동형 안전벨트 리트랙터의 구동 메커니즘 설계

탁태오,박재순,국민구,김대희,신승언,최석 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.A

Motorized retractors enhance the safety of passengers by removing the slack of webbing and by holding upright driving position. Reliability of a driving mechanism that includes one-way clutch between the driving motor and webbing is directly linked to the safety of passenger. In this research, conditions for locking one-way clutch, and also conditions for sustaining locking are theoretically investigated. The operation of a motorized retractor under realistic conditions is simulated in order to validate the proposed retractor design.

모터구동형 안전벨트 리트랙터의 구동 메커니즘 설계

탁태오,박재순,국민구,김대희,신승언,최석 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.B

Motorized retractors enhance the safety of passengers by removing the slack of webbing and by holding upright driving position. Reliability of a driving mechanism that includes one-way clutch between the driving motor and webbing is directly linked to the safety of passenger. In this research, conditions for locking one-way clutch. and also conditions for sustaining locking are theoretically investigated. The operation of a motorized retractor under realistic conditions is simulated in order to validate the proposed retractor design.

프리텐셔너가 장착된 시트벨트 시스템의 관성잠김 안전버클 개발

탁태오,국민구,김대희,박재순,신승언,최석 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.A

To improve passenger safety, seat belt systems with pre-tensioner that tightens seat belt webbing using explosive just before collision are widely used these days. Even though seatbelt must not unlatched without passengers' operation. explosive power of pre-tensioner can cause unlocking of a buckle. To prevent the unlocking, an anti-g mass that blocks displacement of the release button has been attached to the buckle. In this study, the dynamics and statics of locking mechanism associated with operation of anti-g buckle has been theoretically investigated, and important design variables that affect the operation of anti-g buckle have been identified. Through the total seat belt system's dynamic simulation using force and displacement inputs obtained from seat belt sled test, design of the proposed anti-g buckle has been validated.

녹용의 약효 성분에 관한 연구 ( 3 ) 녹용 및 Pantocrin 의 지방산 조성에 관하여

김영은,이승기,이명희,신승언 ( Young Eun Kim,Seung Ki Lee,Myoung Hee Lee,Seung Uon Shin ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.4

The lipid soluble fraction of antler velvet layer (Cervus nippon taiouanus) was extracted and compared to that of pantocrin (ethanol preparation of antler, commericially available). The lipid soluble components(801.5㎎ from antler velvet layer and 979.1㎎/150㎖ from pantocrin) were fractionated into neutral lipids, glycolipids, phosphclipids, gangliosides, and nonlipid components (lipoprotein and peptide) on Sephadex G-25 column followed by silicic acid column chromatography. The percentage composition of the lipid soluble fraction was neutral lipid 65.3%, glycolipid 5.2%, phospholipid 12.9%, ganglioside 6.1 %, nolipid 2.9 %. The neutral lipids were fractionated into squalene, sterolester, triglyceride, free fatty acid, sterol, diglyceride, monoglyceride on boric acid impregnated silicic acid column. The fractions collected were determined by thin layer chromatography. The compositions of the free fatty acid fractions were determined by gas liquid chromatography. The sterolester, triglyceride fractions were hydrolyzed to give free fatty acids with 10% KOH solution, refluxed at 80-85℃ for four hours, and carried out gas liquid chromatography. The free fatty acids in antler velvet layer were found to be nine kinds of saturated fatty acids, nine kinds of unsaturated fatty acids, two branched fatty acids and six unknown components. The main components of antler free fatty sacid were oleic (21.7%), linolenic(31.2%), palmitic(19.6%), stearic(12.1%), palmitoleic(10.2%), and those make 94.4% of antler free fatty acids. In pantocrin free fatty acid fraction, seven kinds of saturated fatty acids, four kinds of unsaturated fatty acids, one branched fatty acid and three unknown compopents were detected. The main components of pantocrin free fatty, acid fraction wereoleic(45%), palmitic(19.5%), lauric(10.9%), stearic(6.6%), palmitoleic(5.6%), myristic(3.4%), capric(3.1%), and those make 94.1% of pantocrin free tfatty acids. The ester fatty acids of triglyceride fraction were found as follows: In antler triglyceride faction, nine kinds of saturated fatty acids, three kinds of unsaturated fatty acids, three branched fatty acids and ten unknown components were detected. In pantocrin triglyceride fraction, it was found to contain five kinds of saturated fatty acids, two kinds of unsaturated fatty acids, one branched fatty acid and ten unknown components. after saponification of triglyceride fraction, two new fatty acids (pentadecylic, oleic) in antler neutral lipid and two new fatty acids (lauric, dodecenoic) in pantocrin neutral lipid were detected. In sterol ester fraction, there were six saturated fatty acids, three unsaturated fatty acids, one branched fatty acid and eight unknown components in antler neutral lipid, and five saturated fatty acid, three unsaturated fatty acids and five unknown components in pantocrin neutral lipid. After saponification of sterol ester fractions, new fatty acids were determined as dodecenoic, lauric, anteiso-pentadecylic, isopalmitic, heptadecanoic in antler sterol ester fraction and dodecenoic, palmitic, heptadecanoic, heptadecenoic in pantccrin sterol ester fraction.