알카리성 포스파타제 측정방법(測定方法)의 비교연구(比較硏究)

김순기 ( Sun Ki Kim ),이창규 ( Chang Kyou Lee ) 대한임상검사과학회 1981 대한임상검사과학회지(KJCLS) Vol.13 No.1

Measurement of actiyity of serum alkaline phosphatase has provided the clinician with valuable aid in confirming his diagnosis of dise2.se of the hepatobiliary and skeletal systems. After demonstration of a serum alkaline phosphatase in 1930 several analytical methods for clinical laboratory were proposed, including those of Bodansky, Bessey-Lowry, and Kind-King. The primary difference among the tests resides in the different substrates employed, although they also differ in substrate concentration, temperature, pH, buffer and incubation times utilized. The major aim of this study was to evaluate three technics for the measurement of serum alkaline phonphatase, to establ1sh their interrelating reliability.

Cholesterol의 효소법(酵素法)에 대한 검토(檢討)

김순기 ( Sun Ki Kim ) 대한임상검사과학회 1979 대한임상검사과학회지(KJCLS) Vol.11 No.1

We have studied the enzymatic method of cholesterol which was developed by Richmond in 1973 and Allain in 1974 comparing to Lieberman Buchard reaction and Kiliani reaction. 1) In enzymatic method there was no harm health because this method does not require the concentrated acid. 2) The volume of specimen required for the test is very small(0.02 ml) as compared to the other methods. (0.5-0.2 ml) 3) The use of autoanalyzer was proper to the method. 4) This method was expensive because of easy 10ss of its activity. This method is superior to the other methods in its sensitivity and reproducibility.

인천지역 대표 중,고등학교 여자 운동선수의 운동경기 종목별 철분 결핍에 관한 상태

정선희 ( Sun Hee Cheong ),성현이 ( Hyun I Sung ),김순기 ( Soon Ki Kim ),장경자 ( Kyung Ja Chang ) 한국운동영양학회 2004 Physical Activity and Nutrition (Phys Act Nutr) Vol.8 No.3

This study was carried out to investigate iron deficiency of elite female adolescent athletes in Inchon area. The subjects were 102 elite female athletes of middle and high schools located in Inchon. Nutrient intakes were obtained a self-reported questionnaire and 3-days recall method. Also anthropometric data and hematological parameters related to iron-deficiency were obtained. Statistical analysis by two-way ANOVA was performed to discriminate the main effects of age (as middle and high school athletes) and sports types, and their interaction. Partial correlations were also calculated to determine associations among the nutrient intakes, anthropometric measurements, exercised-related data and hematological parameters after age and total calorie intake were controlled. High school athletes` hemoglobin (Hb) concentration was significantly lower compared to middle school athletes (p<0.05). Also track and field athletes of middle school showed the lowest Hb concentration and hematocrit (Hct), which were significantly different compared to those of other sports types (p<0.05). More than half of the subjects were iron deficiency (Transferrin saturation<16% or serum ferritin 15 μg/dl). But there was no significant difference in iron deficiency among the athletes of different sports types. There was no significant difference in the daily nutrient intakes and % RDAs between iron deficiency and normal groups. Hb concentration of the subjects showed weak positive correlations with their protein, iron, plant iron and zinc intakes, which were adjusted for age and total energy intakes. However, Hb concentration of the subjects showed weak negative correlation with their usual training time. Hct of the subjects showed weak positive correlation with their total protein and animal protein intake, which were adjusted for age and total energy intakes. But it showed weak negative correlation with their height. The result of this study suggests that elite female adolescent athletes in Inchon area had high frequency of iron deficiency. Therefore, nutrition education and action program are needed to prevent elite female adolescent athletes` health problems such as iron-deficiency and menstrual dysfunction, and to improve athletic performance and nutritional status.

사용자 프로파일과 그룹 프로파일을 이용한 문서순위결정

우선미(Sun-Mi Woo),유춘식(Chun-Sik Yoo),김용성(Yong-Sung Kim),김순기(Soon-Ki Kim) 한국정보과학회 1999 한국정보과학회 학술발표논문집 Vol.26 No.1B

정보의 양과 분야가 기하급수적으로 증가함에 따라 사용자는 최적의 정보를 얻기 위해 어떠한 방법으로 정보 요구를 표현할 것인가와 무수한 양으로 제공되는 검색결과 중에서 어떤 것을 선택해야 하는가에 관한 문제에 직면하게 된다. 본 논문에서는 사용자에게 최적의 검색 결과와 편의성을 제공하기 위하여 사용자 프로파일(user profile)과 그룹 프로파일(group profile)을 생성하여 사용자의 선호도를 표현하고, 통계적 분석 기법을 이용하여 검색 결과 문서의 순위를 결정하는 기법을 제안한다.

인천 지역 초, 중, 고등학생의 B형 간염 바이러스 표면항원 양성률에 대한 조사 연구

장지연,정수진,김순기,손병관,홍영진,홍광선,Chang, Ji Yeon,Jeong, Su Jin,Kim, Soon Ki,Son, Byong Kwan,Hong, Young Jin,Hong, Kwang Sun 대한소아감염학회 2003 Pediatric Infection and Vaccine Vol.10 No.2

목 적 : B형 간염 바이러스 백신은 1983년부터 국내에서 생산되기 시작하여 1985년부터는 보편적으로 사용되면서 B형 간염 바이러스 감염의 예방에 큰 역할을 하고 있다. 그러나 국내의 B형 간염 바이러스 백신의 항체 생성률이 낮다는 보고가 있어, B형 간염 바이러스 백신의 예방 효과에 대한 평가의 필요성이 제기되고 있다. 저자들은 B형 간염 바이러스 백신이 보편적으로 사용되기 시작한 이후 출생한 연령층을 대상으로 인천지역의 HBsAg 양성률을 조사하여 B형 간염 바이러스 백신의 예방 효과에 대해 알아보고자 하였다. 방 법 : 인천시내의 초, 중, 고등학교 학생들을 대상으로 1997, 1998년 2년에 걸쳐 Reversed Passive Hemagglutination(RPHA)법으로 HBsAg을 검사하였으며, 이전에 전국적으로 주로 초, 중, 고등학생을 대상으로 RPHA법으로 조사한 19세 이하 연령군의 HBsAg 검사 결과와 비교하였다. 결 과 : 1) 1997년 초등학생은 0.7%(337/46,861), 중학생은 2.5%(381/15,026), 고등학생은 3.1%(681/21,938)의 HBsAg 양성률을 보였으며 19세 이하의 연령군 전체는 1.7%(1,404/83,984)였다. 2) 1998년 초등학생은 0.6%(257/41,946), 중학생은 2.7%(379/13,652), 고등학생은 2.4%(628/25,277)의 HBsAg 양성률을 보였으며 19세 이하의 연령군 전체는 1.6%(1,266/80,926)였다. 3) 1985년, 1990년과 1995년에 한국건강관리협회에서 RPHA법으로 전국 19세 이하 연령층에서 조사한 HBsAg 양성률은 각각 6.1%, 5.2%, 3.5%였다. 결 론 : 인천지역에서 초, 중, 고등학생의 HBsAg 양성률은 과거의 전국 조사에 비해 낮으며, 특히 초등학생의 경우 현저히 낮은 HBsAg 양성률을 보이고 있다. 이는 1997년도 및 1998년도의 초등학교 6학년 이하에서 많은 수가 출생시 B형 간염 바이러스의 예방 접종을 시작한 연령층으로 이를 HBsAg 양성률의 현저한 감소는 주로 예방 접종의 효과로 생각된다. Purpose : The incidence of hepatitis B virus infection has gradually decreased since 1983 when hepatitis B vaccine was firstly produced. This study was performed to evaluate the efficacy of hepatitis B immunization. Methods : The elementary, middle and high school children in Incheon area were enrolled in this study in 1997 and 1998. Hepatitis B virus surface antigen(HBsAg) was measured using reversed passive hemagglutination(RPHA). Results : The results were as follows The positive rates of HBsAg in elementary, middle and high school children were 0.7%(337/46,861), 2.5%(381/15,026) and 3.1%(681/21,938) respectively in 1997 and 0.6%(257/41,946), 2.7%(379/13,652) and 2.4%(628/25,277) respectively in 1998. The positive rates of HBsAg in children under 19 years of age in 1985, 1990 and 1995 were 6.1, 5.2 and 3.5% respectively. Conclusion : The positive rates of HBsAg in elementary school children in 1997 and 1998 has decreased compared with those of the previous studies. The majority of elementary school children were given hepatitis B vaccination. These results suggest that hepatitis B vaccines used in Korea were effective for prevention of hepatitis B infection in school children.

경인 지역 소아의 A형 간염 항체 보유율

권영세,홍영진,최연호,김순기,손병관,강문수,배수환,홍광선,Kwon, Young Se,Hong, Young Jin,Choe, Yon Ho,Kim, Soon Ki,Son, Byong Kwan,Kang, Moon Su,Pai, Soo Hwan,Hong, Kwang Sun 대한소아감염학회 1998 Pediatric Infection and Vaccine Vol.5 No.2

목 적 : A형 간염은 수년동안 거의 발생 보고가 없었으나 최근 경인지역에서 A형 간염 발생이 증가하였다고 보고되고 있으며, 소아에서도 대전에서 집단 발생이 보고된 바가 있다. 이는 사회경제적으로 발전하고 주거환경이 좋아지면서 소아에서의 A형 간염에 대한 항체 보유율이 낮아진 결과, 감염원에 노출된 연장아와 성인에서 현증 감염이 증가된 것으로 추측된다. 이에 저자들은 경인 지역에서 건강한 소아의 A형 간염 항체 보유율을 조사하여 A형 간염의 역학적 특성을 이해하는데 도움이 되고자 이 연구를 시행하였다. 방 법 : 경인 지역의 l세 76명의 유아와 3세 78명 및 5세 58명의 유아원생과 11세, 13세, 15세, 17세의 초중고생 각각 100명씩을 채혈하였고 혈청을 분리하여 $-80^{\circ}C$에 보관하였다가 Abbott사의 AxSYM$^{(R)}$ HAVAB(Microparticle Enzyme Immunoassay : MEIA)를 이용하여 anti-HAV를 측정하였다. 결 과 : 1) 전부 612명을 검사하였고 남아 316, 여아 296명으로 남녀의 비는 1.1 : 1이었다. 2) 1세군에서는 10.5%(남아 6례, 여아 2 례)에서 anti-HAV가 양성이었고, 3, 5, 11, 13세 군에서는 전부 anti-HAV가 음성이었으며, 15세 군에는 6%(남학생 4례, 여학생 2례)에서 anti-HAV가 양성이었고, 17세 군에서는 11 %(남학생 7례, 여학생 4례)에서 양성이었으며 남녀간의 차이는 없었다. 결 론 : 경인 지역의 유아 및 학동기 소아에서 A형 간염 항체가 거의 형성되어 있지 않으므로 감염자와의 접촉이나 오염된 음식들을 통해 학동기 소아에서의 현증 환자 발생의 위험이 있어, A형 간염에 대한 관심을 가지고 진료에 임하는 것이 필요하며 예방 방법에 대한 지침이 만들어져야 될 것으로 생각된다. Purpose : The incidence of hepatitis A virus infection in Korea has decreased recently. However, the prevalence in children and young adults is on an increasing trend in Inchon-Kyunggi prefecture. Economic development that leads to better living conditions and improves hygiene facilities has reduced the rate of HAV infection. This study was designed to evaluate the recent seroprevalence rate of hepatitis A in children and adolescents. Methods : Study population consisted of 612 children and adolescents aged 1 to 17 years in Inchon-Kyunggi prefecture. Serum samples were collected between 1996 and 1997 and tested for anti HAV by Microparticle Enzyme Immunoassay(MEIA). Results : According to age, the seropositive rates were 10.5%(male 13%, female 6.7%) in 1 year-old group, 0% in 3, 5, 11 and 13 year-old group, 6%(male 8%, female 4%) in 15 year-old group, and 11%(male 14%, female 8%) in 17 year-old group. Conclusion : This study showed that seropositive rate of HAV infection increased with age. While it revealed extremely low incidence of HAV infection in children less than 13 years old, they are also likely 10 be exposed 10 the infection. Our findings suggest that the effective administration of vaccination is needed in selected and high risk groups.

류마티스 관절염에 동반된 빈혈에서 혈청 Transferrin 수용체 측정의 유용성: 골수 저장철과의 비교

김성수 ( Sung Soo Kim ),박원 ( Won Park ),배성권 ( Sung Kwon Bae ),이용환 ( Yong Hwan Lee ),송정수 ( Jung Soo Song ),최종원 ( Jong Weon Choi ),김순기 ( Sun Ki Kim ) 대한류마티스학회 2000 대한류마티스학회지 Vol.7 No.4

Objective: This study was aimed at investigating the usefulness of serum transferrin receptor (sTfR) in anemic patients with rheumatoid arthritis (RA) compared with bone marrow storage iron and other tests for anemia. Methods: Fifty-five anemic RA patients were undergone anemia study including hematologic indices, iron panel, and sTfR. Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were also measured. Eighteen patients performed marrow iron stain. The sTfR and serum ferritin levels were compared with bone marrow iron store, hematologic values, iron batteries, and markers of the disease activity. Results: 1) Mean sTfR concentration was 2.68±1.29mg/L in all patients. 2) sTfR correlated significantly with hemoglobin concentration (r=-0.491; p<0.001), hematocrit (r=-0.348; p=0.009), MCV (r=-0.597; p<0.001), RDW (r=0.696; p<0.001), serum iron (r=-0.389; p=0.003), and transferrin saturation (r=-0.451; p=0.001) 3) Ferritin did not correlated significantly with hemoglobin, hematocrit, serum iron/TIBC, MCV and RDW, except reticulocyte count (r=0.295; p=0.032) and total iron binding capacity (TIBC) (r=-0.503; p<0.001). 4) sTfR showed no significant correlation with ESR and CRP, whereas ferritin correlated with CRP (r=0.342; p=0.019). 5) Among the patients who performed iron staining from bone marrow, sTfR was higher in iron-depleted group compared with iron replete group (p=0.040). Conclusion: This study suggests that measurement of sTfR may be useful assay for anemia and the possible substitute for invasive bone marrow study in differentiating iron deficiency anemia from anemia of chronic diseases in patients with RA.

Cholesterol의 酵素法에 대한 檢討

金淳基 대한임상병리사협회 1979 임상병리사회지 Vol.11 No.1

10∼18세 남녀 학생에 있어서 빈혈의 유병률

김순기,이범석,임문빈,이해선 大韓血液學會 1998 大韓血液學會誌 Vol.33 No.2

부천시 초중고 학생에서 철결핍과 철결핍성빈혈의 빈도 및 식이와의 관계

김순기,임대현,최연호,전용훈,홍영진,손병관,배수환,이해선 大韓血液學會 1998 大韓血液學會誌 Vol.33 No.2