시험관내 및 생체내로 투여한 카드뮴이 랏트의 간, 신 및 고환조직 내의 Superoxide Radical, Superoxide Dismutase, Catalase 및 ATPase 활성도에 미치는 영향

김성무,정규철,Kim, Sung-Moo,Chung, Kyou-Chull 대한예방의학회 1990 Journal of Preventive Medicine and Public Health Vol.23 No.4

Production of free radicals of superoxide anion in tissues by cadmium, activities of superoxide dismutase and catalase to protect tissue damages caused by the free radicals and ATPase that plays an important role in energy metabolism at cellular level were investigated. Experiments in vivo were conducted with liver, kidney and testicle tissue homogenates of rats adding $0.05{\sim}0.50mM$ cadmium chloride, and in vivo experiments administering single dose of 5 mg of cadmium/kg of body weight in 0.1% cadmium chloride solution intraperitoneally 48 hours prior to evisceration. Production of superoxide radicals in liver and testicle increased with addition of cadmium in vitro, but not in kidney. In vivo experiments, however, superoxide radicals slightly increased in liver and kidney but not in testicle. Superoxide dismutase (Cu, Zn-SOD and Mn-SOD), catalase and ATPase (total, $Mg^{++}-\;&\;Na^+,\;K^+-$) activity decreased in the presence of cadimium in dose dependent manner. Reduction of these enzyme activities varied not only with dosage of cadmium but also with type of tissue and between in vitro and in vivo experiment.

금속단백분해효소의 활성 저해를 통한 삼귀고의탕의 전이억제 효과

김성무(Sung Moo Kim),이연희(Yun Hee Rhee),이주호(Joo Ho Lee),김성훈(Sung Hoon Kim),이은옥(Eun Ok Lee) 한의병리학회 2008 동의생리병리학회지 Vol.22 No.6

소화기 영역에서 Epithelial-mesenchymal Transition의 역할

김성무 ( Sung Moo Kim ),한정호 ( Joung Ho Han ),박선미 ( Seon Mee Park ) 대한소화기학회 2010 대한소화기학회지 Vol.56 No.2

The epithelial-mesenchymal transition (EMT) plays physiologic roles in the embryogenesis, wound healing, and tissue regeneration. In terms of pathological direction, it causes organ fibrosis, cancer development, progression, metastasis, and chemoresistance. Recently, the underlying mechanism of EMT and many kinds of EMT regulators have been identified. Pharmaceutical treatment strategies which target EMT pathway could be applied for the prevention of tissue fibrosis and cancer progression. In the field of gastroenterology, profuse evidences have been collected about the critical roles of EMT in cancers of the gastrointestinal tract, liver, and pancreas and hepatic fibrosis. However, EMT varies widely among cancer types, and much remains to be identified about the main regulators of EMT in a specific disease. In this review, we present recent research results regarding the roles of EMT in cancers and organic fibrosis, especially in the area of gastroenterology. (Korean J Gastroenterol 2010;56:69-77)

증례 : 알레르기 ; 박트림에 의한 아나필락시스 1예

김성무 ( Sung Moo Kim ),김경숙 ( Kyung Sook Kim ),김미경 ( Mi Kyung Kim ) 대한내과학회 2011 대한내과학회지 Vol.80 No.3

내원 5년 전 우측 신장 이식술 시행 후 박트림을 복용하였던 만성신부전 환자가 최근 만성중이염으로 박트림을 복용 후 전신두드러기, 혈관부종과 저혈압을 경험하여, 치료 후박트림을 복용하지 말라고 교육하였지만, 만성중이염이 지속되어 환자 스스로 박트림을 다시 복용하여 저혈압과 혈관부종, 전신 두드러기 등 증상이 재발하였다. 박트림으로 피부단자 검사를 시행하여 강양성반응이 나타나 박트림에 의한 아나필락시스임을 확진하였으며 면역효소법과 면역효소억제능 검사 결과 박트림의 다섯 가지 구성성분 중 sulfamethoxazole이 원인 알레르겐임을 확인하였다. Bactrim consists of the sulfonamides trimethoprim and sulfamethoxazole. These induce relatively frequent adverse drug reactions, including allergic reactions ranging from urticaria to anaphylaxis. Either component can be the causative allergen, so it is necessary to determine which has caused an allergic reaction to prevent further allergy. We report the case of a 46-year-old male with chronic renal failure who experienced anaphylactic shock twice after ingesting trimethoprim-sulfamethoxazole, as was proven by the medical history and skin prick testing. Enzyme-linked immunoassays and enzyme-linked allergen inhibition assays for allergen-specific IgE antibody for the five components of Bactrim showed that sulfamethoxazole was the causative allergen. (Korean J Med 2011;80:365-369)

단호박의존성 운동유발성 아나필락시스

김성무 ( Sung Moo Kim ),유숙희 ( Suk Hee Yoo ),김미경 ( Mi Kyeong Kim ) 대한천식알레르기학회 2011 천식 및 알레르기 Vol.31 No.2

Food-dependent exercise-induced anaphylaxis (FDEIA) is a kind of exercise-induced anaphylaxis, which is characterized by anaphylactic symptoms only after eating certain foods. In Korean patients, wheat flour, apple, parsley, and crown daisy have been reported as causative foods. We report the case of an 18-year-old male with squash-dependent, exercise-induced anaphylaxis. The patient had urticaria, angioedema and chest discomfort during walking after taking roasted pork belly, green onion, lettuce, sesame leaf, mushroom and squash. He had also experienced the same event 4 months before this presentation. Skin prick test showed a strong positive reaction only to squash. Exercise provocation test turned out to be a negative result. However, exercise provocation test performed 30 minutes after ingestion of boiled squash resulted in hives with itching. Even though exercise provocation test performed just after ingestion of squash did not show any typical anaphylaxis reactions, this patient can be diagnosed with squash-dependent, exercise-induced anaphylaxis, based on his typical medical history and development of urticaria after the provocation test. (Korean J Asthma Allergy Clin Immunol 2011;31:140-143)

재래감귤 팔삭의 과피 추출물이 LPS로 활성화 된 RAW264.7 대식세포에서 염증매개물질 억제에 미치는 효과

김철원,김성무,정승원,김소미,안광석,Kim, Chul-Won,Kim, Sung-Moo,Jeong, Seung-Weon,K., So-Mi Cho,Ahn, Kwang-Seok 대한암한의학회 2011 大韓癌韓醫學會誌 Vol.16 No.2

Objectives : Citrus is the fruit that is readily available around us. Therefore, we investigated the anti-inflammatory effects of fraction isolated from the Citrus hassaku pericarp in RAW264.7 macrophage cells. Methods : The effects of fraction from Citrus hassaku pericarp on cell viability on RAW264.7 cells were measured by the MTT assay. The mRNA levels of iNOS and COX-2, its protein level by fraction of Citrus hassaku pericarp treatment in RAW264.7 macrophage cells were investigated by RT-PCR and immunoblots. Nitrite accumulation in the culture was measured colorimetrically by the Griess reaction using a Griess reagent. The amount of IL-6 and TNF-${\alpha}$ production was determined using an enzyme-linked immunosorbent assay (ELISA) kit. Results : The results indicated that the fraction of Citrus hassaku pericarp concentration highly suppressed lipopolysaccharide (LPS)-induced nitric oxide (NO) and IL-6 productions without a cytotoxic effect on RAW264.7 cells. fraction of Citrus hassaku pericarp inhibited the expressions of LPS-induced iNOS and COX-2 protein and their mRNA in a dose-dependent manner. Particularly, fraction of Citrus hassaku pericarp suppressed the level of nuclear factor-${\kappa}B$ (NF-${\kappa}B$) activity, which was linked with the suppression of LPS-induced phosphorylation of p65 at serine 276 and p65 translocation into nuclei, but not MAPK signaling. In addition, treatment with fraction of Citrus hassaku pericarp inhibited the production of IL-6 and TNF-${\alpha}$ in LPS-stimulated RAW264.7 cells. Conclusion : Our results indicate that fraction of Citrus hassaku pericarp potentially inhibits the biomarkers related to inflammation through the blocking of NF-${\kappa}B$ p65 activation, and it may be a potential therapeutic candidate for the treatment of inflammatory diseases.

전립선 암세포주 DU145의 세포고사 유도를 통한 신선초 (Angelica keiskei) 메틸렌 클로라이드 추출물의 항암효과

강윤묵,김성무,김현중,박경란,심범상,김성훈,최승훈,안규석,안광석,Kang, Yoon-Mook,Kim, Sung-Moo,Kim, Hyun-Jung,Park, Kyung-Ran,Shim, Bum-Sang,Kim, Sung-Hoon,Choi, Seung-Hoon,Ahn, Kyoo-Seok,Ahn, Kwang-Seok 대한암한의학회 2010 大韓癌韓醫學會誌 Vol.15 No.1

The roots and leaves of Angelica keiskei (AK) have been used for the treatment of various diseases including coronary heartdisease, hypertension, and cancer in the Korean folk medicine. However, the mechanism by which methylenechloride fraction (MF) from AK exerts anti-tumorigenic activity in human prostate cancer cells has not been fully understood. In the present study, we report the MF exerted the highest cytotoxicity against prostate cancer DU145 cells compared with other fractions. Especially, MF caused the accumulation of sub-G1 DNA contents of cell cycle and increased annexin V-positive apoptotic bodies and DNA fragmentation. MF down-regulated several proliferative (Cyclin D1) and anti-apoptotic (Bcl-xl, Bcl-2, IAP-1/2, and survivin)gene products in these cells. Hence, MF induced apoptosis through the caspase-3 activation in DU145 cells. We further confirmed that caspase-3 plays an importance role in MF-induced apoptosis in DU145 cells by using caspase-3 inhibitor. Additionally, we observed that MF potentiated Dox-induced apoptosis in DU145 cells. Taken together, our data demonstrate the evidence that MF induces apoptosis depend on caspase-3 activation of and overcomes resistance to chemotherapy in human prostate cancer cells.

운동유발성 천식을 동반한 콜린성 두드러기

윤태영 ( Tae Young Yoon ),김성무 ( Sung Moo Kim ),유숙희 ( Sook Hee You ),김미경 ( Mi Kyeong Kim ) 대한천식알레르기학회 2011 천식 및 알레르기 Vol.31 No.2

Exercise-induced asthma that presents with typical symptoms, such as shortness of breath, chest tightness and cough, occurs after exercise. Cholinergic urticaria occurs due to the conditions that increase core body temperature, such as exercise, hot bath, sauna or emotional stress. A 21-year-old male experienced wheezing, dyspnea and urticaria on his whole body after exercise at the age of 12. Thereafter, he experienced pinpoint-sized urticaria after exercise, sauna or emotional stress as well as during outdoor activities in summer. Wheezing and dyspnea sometimes appeared without any skin rashes after light exercise, while skin rashes occurred only after sauna. The result of treadmill exercise test was positive: FEV1 decreased by 36% relative to that of the baseline value and pinpoint-sized urticaria appeared on his trunk, back and face. Intradermal injection of 0.1 mL of methacholine (0.01 mg/mL) produced hives. Based on these results, we confirmed that cholinergic urticaria was accompanied by exercise-induced asthma in this patient. (Korean J Asthma Allergy Clin Immunol 2011;31:153-156)

원인불명의 담즙흉

성문혁 ( Mun Hyuk Seong ),김성무 ( Sung Moo Kim ),유숙희 ( Suk Hee Yoo ),박우리 ( Woo Ri Park ),안진영 ( Jin Young An ),최강현 ( Kang Hyeon Choe ),이기만 ( Ki Man Lee ),김시욱 ( Si Wook Kim ) 대한결핵 및 호흡기학회 2011 Tuberculosis and Respiratory Diseases Vol.70 No.3

Cholethorax is a bilious pleural effusion caused by a pleurobiliary fistula or leakage of bile into the pleural space. Most cases of cholethorax arise from a complication of abdominal trauma, hepatobiliary infection, or invasive procedures or surgery of hepatobiliary system. However, we experienced a case of a patient with cholethorax of unknown origin. There was no evidence of pleurobiliary fistula or leakage of bile from the hepatobiliary system although we examined the patient with various diagnostic tools including chest and abdominal computed tomography, endoscopic retrograde cholangiopancreatography, tubography, bronchofiberscopy, hepatobiliary scintigraphy and video-assisted thoracoscopic surgery. Herein we report a case of cholethorax for which the specific cause was not identified. The patient was improved by percutaneous drainage of pleural bile.

흰쥐 결장운동에서 Neurotensin의 수축작용과 자율신경계의 영향

지정훈 ( Jeong Hoon Ji ),김성무 ( Sung Moo Kim ),서의근 ( Euikeun Seo ),조영심 ( Young Shim Cho ),유숙희 ( Suk Hee Yoo ),한정호 ( Joung Ho Han ),채희복 ( Hee Bok Chae ),박선미 ( Seon Mee Park ),윤세진 ( Sei Jin Youn ) 대한장연구학회 2010 Intestinal Research Vol.8 No.2

Background/Aims: Although neurotensin (NT) stimulates colon motility and the passage of intestinal contents, the associated mechanism of action remains unclear. The objective of this study was to investigate the effects of NT on colon motility using isolated rat colon. Methods: Intraluminal pressure was measured at both the proximal and distal portions of the isolated colon. An isolated rat colon was perfused with Krebs solution via the superior mesenteric artery. After stabilization, NT was administered in concentrations of 14, 28, 138 and 276 pM. After pretreatment with phentolamine, propranolol, hexamethonium, atropine or tetrodotoxin, NT was administered at a concentration of 276 pM, and then the intraluminal pressure was monitored. Results: NT significantly increased colon motility at concentrations of 14, 28, 138, and 276 in the proximal colon (25.1±6.5%, 175.4±117.0%, 240.8±115.1% and 252.3±110.6%, respectively) and in the distal colon (35.6±11.8%, 97.5±35.1%, 132.7±36.7% and 212.1±75.2%, respectively). The stimulant effect of NT was more potent in the proximal colon, in a concentration-dependent manner (P<0.05). The stimulant effect of NT was significantly inhibited by atropine at both the proximal and distal colon and by tetrodotoxin at the proximal colon, but not by tetrodotoxin at the distal colon and not by propranolol, phentolamine, or hexamethonium at both the proximal and distal colon. Conclusions: NT increased colon motility at both the proximal and distal portions of the rat colon. The effects were more prominent at the proximal portion. The results of this study suggest that the stimulant action of NT may be mediated by local cholinergic muscarinic receptors. (Intest Res 2010;8:162-171)