발효 , 세포배양 , 생물공정 Red Beet 의 모상근 배양을 이용한 천연색소인 Betacyanin 생산의 최적화

김선희,김성훈,이주노,안상욱,김광수,황백,이현용 ( Sun Hee Kim,Sung Hoon Kim,Ju No Lee,Sang Wook An,Kwang Soo Kim,Baik Hwang,Hyeon Yong Lee ) 한국미생물생명공학회 1998 한국미생물·생명공학회지 Vol.26 No.5

건축구조용강재(SN490) 조립 H형강 기둥-보 접합부의 이력거동에 관한 실험적 연구

김선희,이성희,김진호,김대중,최성모,Kim, Sun Hee,Lee, Seong Hui,Kim, Jin Ho,Kim, Dae Jung,Choi, Sung Mo 한국강구조학회 2008 韓國鋼構造學會 論文集 Vol.20 No.6

1994년 노스리지 지진 이전 우수한 연성능력을 보유한 내진상세로 생각되던 WUF-B 접합부의 기둥-보플랜지 용접위치에서 발생 한 취성파괴는 WUF-B 접합부에 대한 새로운 고찰을 요구하였다. FEMA(Federal Emergency Management Agency)의 후원으로 SAC Steel Project에서는 WUF-B 접합부의 스캘럽(Weld access holes) 형상, 용접과정, 용접재료 등을 개정한 내진상세를 FEMA-350에 제안하 였다. AISC Seismic Provisions(2005)에서는 WUF-B 접합부를 OMF(Ordinary Moment Frames)로만 사용하도록 규정하고 있다. 본 연 구에서는 SM490 및 SN490 조립형강을 이용하여 FEMA-350에 제시된 WUF-B 상세 규정을 따라 강종(SM, SN), 보플랜지두께, 보의 춤을 변수로 기둥-보 접합부 실대 실험체 2개를 제작하여 실험을 수행하였으며, 내진성능 평가를 위한 가력 방법 및 내진성능을 평가하였다. JB-1, JB-2 실험체는 AISC Seismic Provisions(2005)에서 제시하고 있는 OMF와 SMF 내진성능을 만족하는 결과를 나타났으며, 보플랜지 두께와 보의 춤에 따른 WUF-B 접합부의 내진성능이 재정의 될 필요가 있는 것으로 사료된다. The brittle failure where is occurred the welding position of column-beam flange of WUF-B connection that consider about a seismic detail possess a superior ductility capacity before Northridge earthquake 1994, require newly study about WUF-B connection. SAC Steel Project suggests a seismic detail to FEMA-350 by supporting of FEMA. It revise shape of weld access holes of WUF-B connection, welding processand welding material etc, In spite of these revision, AISC Seismic Provisions (2005) prescribe WUF-B connection using an only OMF. Recently in Korea, as the earthquake of about seismic intensity 5 occur, the necessity of revision for connection seismic detail comes to the front in Korea and FEMA-350 connection seismic details are going to include in KBC-2008 as it is. In this study, two column-beam connection specimens were marked by using SM490, SN490 built-up H-section, and based on WUF-B detail prescription of FEMA350. The parameters of the specimens are types of steel (SM, SN), and evaluate the capacities of structure and seismic by experiment. Finally we confirm a superior ductility capacity aboutspecimens JB-1 and JB-2, using SM490 and SN490,and these specimens had sufficient OMF and SMF seismic capacity, as indicated in AISC Seismic Provisions (2005).

Metal Organic Chemical Vapor Deposition법을 이용한 Germanium 전구체의 증착 특성 연구

김선희,김봉준,김도형,이준기,Kim, Sun-Hee,Kim, Bong-June,Kim, Do-Heyoung,Lee, June-Key 한국재료학회 2008 한국재료학회지 Vol.18 No.6

Polycrystalline germanium (Ge) thin films were grown by metal organic chemical vapor deposition (MOCVD) using tetra-allyl germanium [$Ge(allyl)_4$], and germane ($GeH_4$) as precursors. Ge thin films were grown on a $TiN(50nm)/SiO_2/Si$ substrate by varying the growth conditions of the reactive gas ($H_2$), temperature ($300-700^{\circ}C$) and pressure (1-760Torr). $H_2$ gas helps to remove carbon from Ge film for a $Ge(allyl)_4$ precursor but not for a $GeH_4$ precursor. $Ge(allyl)_4$ exhibits island growth (VW mode) characteristics under conditions of 760Torr at $400-700^{\circ}C$, whereas $GeH_4$ shows a layer growth pattern (FM mode) under conditions of 5Torr at $400-700^{\circ}C$. The activation energies of the two precursors under optimized deposition conditions were 13.4 KJ/mol and 31.0 KJ/mol, respectively.

행위자의 주관과 객관의 이중성은 어떻게 양립가능한가?

김선희(Sun-Hie Kim) 한국철학회 2017 철학 Vol.0 No.133

주관적이면서 객관적이기도 한 인간의 이중적 본성이 양립한다는 것을 어떻게 설명할 수 있는가? 김재권은 행위론에서 바로 이 문제를 제기한다. 인간 행위에 대한 기존의 대표적인 이론들, 인과론과 비인과론은 행위를 바라보는 주관적 관점과 객관적 관점을 이원적으로 분리하거나 어느 한쪽으로의 환원을 시도한다. 필자는 주관과 객관의 양립가능성 문제에 접근하는 기존의 모델들을 비판적으로 검토한 후 새로운 대안을 제안할 것이다. 여기서 이 문제를 해명하기 위한 시도의 하나로 김재권의 투사 및 시뮬레이션 모델을 검토하고 그것이 갖는 설명력과 한계를 논의한다. 나아가 필자가 고안한 시뮬레이션 집단사고실험 모델은 그러한 한계를 해결할 수 있다는 것을 보임으로써, 주관과 객관의 이중성을 해명하는 하나의 모델이 될 수 있다는 것을 논의할 것이다. How can we explain that the dual nature of human beings, both subjective and objective, is compatible? Kim Jaegwon poses this problem from his action theory. Existing representative theories of action, causal theory and non-causal theory, attempt to divide the subjective viewpoint and the objective stance of agents into two, or try to reduce to one side. I will critically review existing models approaching the issue of compatibility between subjectivity and objectivity and suggest new alternatives. Here, I examine Kim’s projection thesis and simulation model and discuss its explanatory power and limitations. Furthermore, I will argue that the model of group simulation thought experiments I designed can be a model for explaining our dual nature of subjectivity and objectivity by showing that it can solve such limitations.

검정콩의 주요 항산화 원인물질 및 항산화 효과의 비교

김선희,권태완,이영순,정명근,문갑순,Kim, Sun-Hee,Kwon, Tai-Wan,Lee, Young-Soon,Choung, Myoung-Gun,Moon, Gap-Soon 한국식품과학회 2005 한국식품과학회지 Vol.37 No.1

검정콩에 함유되어 있는 성분들 중 주요 항산화 원인물질을 구명학기 위하여 청자콩의 isoflavone, 토코페롤, phenolic acids 및 안토시아닌의 함량을 측정하였다. 그 결과 genistein이 43.86mg%, daidzein이 31.73mg% 함유되어 있었으며, 토코페롤 함량은 ${\alpha}$-토코페롤이 1.99mg%, ${\beta}$-토코페롤 0.47mg%, ${\gamma}$-토코페롤 10.68mg%, ${\dalta}$-토코페롤 3.95mg%이 함유되어 있었다. 11종류의 phenolic acids에서는 benzoic(126.70mg%)>p-coumaric(67.68)>salicylic(59.40)>gentisic(43.19)>ferulic(16.57)>syringic(15.04)>chlorogenic(8.00)>caffeic(4.53)>vanillic(2.82)>p-OH benzoic(2.47)>trans-cinnamic acid(1.00)의 순으로 함유되어 있었으며, 검정콩 종피에 함유되어 있는 안토시아닌을 측정한 결과 delphinidin 3-glucoside가 1.42, cyanidin 3-glucoside 5.77 및 petunidin 3-glucoside가 0.30mg/g이었다. 검정콩에 함유되어 있는 항산화 물질들 (안토시아닌, isoflavone, phenolic acids 및 토코페롤)을 검정콩에 들어있는 함량과 비례하도록 조제하여 TEAC법으로 항산화 효과를 측정한 결과, genistein, gentisic acid, ${\gamma}$-토코페롤, 안토시아닌의 항산화 효과가 높은 것으로 나타났다. 네 종류의 항산화 물질들의 시너지 효과를 분석한 결과, 이들은 서로 시너지 효과가 있는 것으로 나타났으며 특히 안토시아닌을 혼합할 때 시너지 효과가 가장 높게 나타나 검정콩의 항산화 효과에 안토시아닌이 크게 관여하고 있음을 알 수 있었다. Contents of isoflavone, phenolic acids, tocopherol, and anthocyanin in black soybean (Glycine max) were measured by HPLC. To compare antioxidative activities of main black soybean components, antioxidative effects of the same levels of commercial standard components were measured by Trolox equivalent antioxidant capacity assay (TEAC). Most effective component was gentisic acid followed by anthocyanin, p-coumaric acid, ferulic acid, genistein, syringic acid, and daidzein. TEAC assay results revealed genistein in isoflavone, gentisic acid in phenolic acids, p-tocopherol in tocopherol, and anthocyanin showed highest antioxidative and synergistic acitivities, with anthocyanin showing strongest synergy effect.

소프트웨어 컴포넌트 이해를 위한 데이터 북 구성

김선희,최은만,Kim, Seon-Hui,Choe, Eun-Man 한국정보처리학회 2002 정보처리학회논문지D Vol.9 No.3

소프트웨어 위기를 극복하기 위하여 컴포넌트 기술이 제안되어 개발에 적용되고 있다. 소프트웨어 컴포넌트는 하드웨어의 집적회로와 같은 블랙박스로 취급되지만 사용자가 효과적으로 이해할 수 있도록 지원되지 않는다면 이용할 수가 없다. 이 논문은 하드웨어 컴포넌트의 이해를 돕기 위한 데이터 북 형식이 소프트웨어 컴포넌트를 표현하는데 잘 적용될 수 있다는 것을 보였다. 데이터 북의 내용으로 UML과 API모형을 도입하여 컴포넌트를 이해하는 접근 방법을 채택하였으며 소프트웨어 컴포넌트의 중요한 부분인 아키텍처와 인터페이스 부분을 추가하였다. 실증적인 실험을 위하여 제안한 데이터 북을 EJB의 배치 디스크립터에 확장 포함하여 컴포넌트 데이터북을 웹 브라우저에서 볼 수 있도록 하였고 그 효용성을 실험하였다. Component technology was proposed and applied to software development to overcome software crisis. Software component is a black box like an integrated circuit in hardware but it can not be utilized without good support specially for helping users understand efficiently. This paper shows that data book format for understanding hardware component can be well applied to representing software component. We selected an approach to understand component by matching the contents of data book with UML and API model technique. Besides, we added the architecture part and the interface which are the most important property of software component to the data book for software components. In order to verify effectiveness of components data book we extended batch descriptor in EJB and performed an experiment providing data book to programmers with components.

홍국발효 대두의 항산화 및 항당뇨 활성

김선희,강순아,Kim, Sun Hee,Kang, Soon Ah 한국식품영양학회 2021 韓國食品營養學會誌 Vol.34 No.2

This study was performed to investigate the antioxidant and antidiabetic activities of soybean fermented with Monascus. Also, the changes in the content of isoflavones and Monacolin K were analyzed. It was observed that the glycoside forms of daidzin and genistin were converted to aglycones of daidzein and genistein within 6 days of fermentation. The product can be used as a health functional material that can increase bioavailability. Monacolin K production was found to increase significantly with the progression of fermentation with an increase to 0.04 mg/g and 0.44 mg/g on 6 and 12 days of fermentation, respectively. The DPPH radical scavenging activity of soybean fermented with Monascus was significantly increased compared to that of soybean. The protein expression of inflammation-related genes (TNF-α, IL-6, and COX-2) in the MIN cell was significantly increased in the presence of alloxan compared to the normal group, but a decrease was observed in the presence of soybean fermented with Monascus. In conclusion, soybean fermented with Monascus showed the highest antidiabetic and antioxidant effects. These results suggest that soybean fermented with Monascus has the potential to be used as a beneficial ingredient with antidiabetic and antioxidant effects.

균형성과표를 이용한 노인장기요양 방문간호센터의 핵심성과지표 개발

김선희,임지영,Kim, Seonhee,Lim, Ji Young 한국가정간호학회 2021 가정간호학회지 Vol.28 No.2

Purpose: The purpose of this study was to develop effective management indicators for improving efficiencies of visiting nursing centers. Method: This was a methodological research study to develop the key performance indicators based on balanced score cards for long-term care visiting nursing centers. The main methods used in this study were literature review, focus group interview, and content validity index. The data analysis was used frequency, percentage, mean, and standard deviation. Results: The common vision of the long-term care visiting nursing centers was identified as "The healthy visiting nursing center to serve high quality cares." Eight action strategies and 15 key performance indicators to achieve this vision were developed. Conclusion: Based on the results of this study, we suggest that the developed balanced score cards will be used as an effective managerial guideline to improve performances of long-term care visiting nursing centers.

Bacillus atrophaeus DYL-130이 생산하는 biosurfactant의 분리 및 특성

김선희,이상철,박인혜,유주순,주우홍,황철원,최용락,Kim Sun-Hee,Lee Sang-Cheol,Park In-Hye,Yoo Ju-Soon,Joo Woo-Hong,Hwang Cher-Won,Choi Young-Lark Korean Society of Life Science 2005 생명과학회지 Vol.15 No.5

원유 분해능이 강력한 균주를 얻고자 덕유산의 토양으로부터 crude oil을 탄소원으로 이용하는 수십 종을 분리하였다. 분리된 균주 중 원유분해능 및 biosurfactant 생성능이 우수한 균주를 선별하여, 형태학적 특성을 관찰하고 165 rDNA sequence와 』gyrA gene sequence를 통하여 Bacillus atrophaeus DYL-130으로 동정하였다. 동정된 균주 배양액의 표면장력은 최저 28 mN/m까지 감소되었다. 또한 Bacillus atrophaeus DYL-130이 생산하는 biosur-factant가 column chromatography에 의하여 분리 되었으며, 분리된 biosurfactant의 toluene 유화능 및 crude biosurfactant의 유화활성과 안정성이 시험 되었다. Crude biosurfactant의 유화활성은 kerosene에서 최대였으며, soybean oil에서도 높은 편이였으나 유화안정성의 경우 soybean oil을 기질로 하였을 경우가 kerosene을 기질로 하였을 때 보다 우수하였다. 또한 crude biosurfactant의 유화안정성을 합성계면활성제와 비교 한 결과 DYL-130이 생산하는 biosurfactant의 유화안정성이 합성계면활성제와 유사하거나 뛰어남을 확인하였다. Column chroma-tography에 의하여 분리된 biosurfactant는 drop-collapsing method에 의하여 surface-activity가 확인 되었으며 또한 toluene에 대한 유화력이 매우 뛰어난 것을 확인 하였다. 따라서 DYL-130에서 추출한 biosurfactant는 합성계면활성제를 대체할 수 있는 환경친화적인 생물 계면활성제로 사용될 수 있는 가능성을 보여주고 있다. 산업적으로 이 물질을 이용하기 위해서 Bacillus atrephaeus DYL-130이 생산하는 biosurfactant에 대한 구조분석과 물리 화학적 특성, 생분해도 및 환경독성 등의 조사를 수행하여야 할 것으로 생각된다. The objective of this study was investigate the characteristic of biosurfactant produced from the iso-lated strain. The strain was isolated from soli samples of Duck-Yu Mountain and it was identified as Bacillus atrophaeus DYL-130 by 16S rDNA and gyrA gene nucleotide sequence analysis. The surface ten-sion of culture filtrate of Bacillus atrophaeus DYL-130 decreased to 28 mN/m and its biosurfactant con-centration was determined by diluting the culture filtrate until the critical micelle concentration (CMC). The emulsifying activity and stability of crude biosurfactant was measured by using water-immiscible hydrocarbons and oils as substrate. The biosurfactant was purified by affinity chromatography and the surface activity of purified biosurfactant was measured by drop-collapsing method and it could be effectively emulsify toluene.

일반인에 있어서 측두하악장애의 인지도 조사에 대한 연구

김선희,Kim, Sun-Hee 대한안면통증구강내과학회 2000 Journal of Oral Medicine and Pain Vol.25 No.4

This study was performed to research how predominantly TMD is known among people and how exactly it is understood. The data set up by this study could be used as beneficial references. Based on this data, people can be more knowledgeable of TMD so that they can recognize the signs and symptoms of TMD. Then, the patients can visit eligible clinician, TMD professionals. Nine hundred thirty six people (426 men and 510 women, ranging from 18 to 69 years old) were selected for the subjects of this study and were investigated by use of self-administered questionnaire. The obtained results were as follows : 1. 10.68% of the subjects were reported to have heard of the term, "Temporomandibular disorders". Females occupied significantly higher percentage than males and 18-29 age group occupied higher percentage than 30-49 age and 50-69 age groups. On the other hand, even more people (52.24%) were reported to have heard of the tenn, "Jaw joint disease". It also had same sex and age prevalences, too. 2. As for the route through which people have heard of TMD, more than half (58.38%) of the people mentioned mass media of communication (T.V. radio 40.36%, newspapers magazines 15.86%, internet 2.16%). Other people who had already heard of TMD (25.05%), dentist (7.75%), and other sources (8.83%) such as physicians, physicians of Chinese medicine, pharmacists were also mentioned. There were not significant differences among sex and age groups. 3. When it comes to the cause and concept of TMD, 32.59% of the subjects considered" an inappropriate overuse of the mandible" as the cause of TMD. There were not significant differences among sex and age groups. 4. The most frequently reported presumable TMD signs and symptoms were jaw pain (61.00%), jaw joint sound (57.80%), and difficulty with mouth opening (50.11%). 5. In answer to the question who is the eligible person to treat jaw joint disease, 35.26% answered the dentists are and 41.99%, orthopedicians. Of the people who chose dentist, 30-49 age group occupied the largest part. In the case of orthopedicians, 18-29 age group was the largest. 6. Of the 7.69% of subjects who had been treated of TMD, only 5.02% of them visited the dentist for the treatment of TMD. There were not significant differences among sex and age groups. 7. In answer to the question of how to prevent development of TMD, 58.87% selected "Avoid eating hard food", 58.65%, "Avoid opening the mouth wide", and 51.07%, "Avoid chewing gum frequently".