Molecular Cloning and Sequence of URA5 Gene Encoding Orotate Phosphoribosyl Transferase(OPRTase) from Entomopathogenic Fungus, Metarhizium anisopliae

HWANG, CHER WON,LEE, DONG KYU,KANG, SUN CHEOL 한국미생물 · 생명공학회 1998 Journal of microbiology and biotechnology Vol.8 No.3

A ura5 gene encoding Orotate Phosphoribosyl Transferase (OPRTasc) of Metarhizium anisopliae was cloned by PCR methods and sequenced. The sequenced ura 5 gene encodes a polypeptide of 234 amino acid residues. This deduced amino acid sequence showed high similarity to other fungi OPRTase and there was no intron sequence between ATG starting codon and TGA ending codon.

Antifungal and Plant Growth Promotion Activities of Recombinant Defensin Proteins from the Seed of Korean Radish (Raphanus sativus L.)

Hwang, Cher-Won The Korean Society of Environmental Agriculture 2009 한국환경농학회지 Vol.28 No.4

In the present study, we analyzed the defensin protein deduced from Korean radish (Raphanus sativus L.) seeds.To express the genes in E. coli, we constructed a recombinant expression vector with a defensin gene, named rKRs-AFP gene isolated from Korean radish seeds. Over expressed rKRs-AFP proteins was separated by SDS-PAGE to determine the purity, and protein concentration was determined by the Bradford method. Antifungal activity was assessed by disk assay method against the tested fungi. As a result, when 500 mL of cell culture were disrupted by sonicator, 32.5 mg total proteins were obtained. The purified protein showed a single band on SDS-PAGE with estimated molecular weight about 6 KDa, consistent with the molecular mass calculated from the deduced amino acid sequence. The purified rKRs-AFP protein showed remarkable antifungal activities against several fungi including Aspergillus niger, Botrytis cinerea causing the gray mold disease, and Candida albicans. In field tests using the purified rKRs-AFP protein, the protein showed the reducing activity of disease spot and the mitigating effect of spreading of disease like agrichemicals. The immuno-assay of rKRs-AFP protein showed that the purified protein entirely accumulated at B. cinerea cytoplasm through the hyphal septa shown by fluorescence imaging. There was no fluorescence inside the cell, when the hypha was incubated without the protein. These all results indicate that the recombinant rKRs-AFP proteins can be utilized as a potential antifungal drug to control harmful plant fungal pathogens.

A Partial Nucleotide Sequence of Chitin Synthase ( CHS ) Gene from Rice Blast Fungus , Pyricularia oryzae and Its Cloning

Cher Won Hwang,In Cheol Park,Won Hae Yeh,Masamchi Takagi,Jin Chang Ryu 한국미생물생명공학회 1997 Journal of microbiology and biotechnology Vol.7 No.2

Molecular Cloning and Sequence of URA 5 Gene Encoding Orotate Phosphoribosyl Transferase ( OPRTase ) From Entomopathogenic Fungus , Metarhizium anisopliae

Cher Won Hwang,Dong Kyu Lee,Sun Cheol Kang 한국미생물생명공학회 1998 Journal of microbiology and biotechnology Vol.8 No.3

N/A

Antifungal Activity of Korean Radish (Raphanus sativaus L) Extracts Against Pathogenic Plant

Won, Hwang-Cher- Korean Society of Life Science 2003 생명과학회지 Vol.13 No.2

국내산 무의 추출물을 여러가지 Column과 HPLC를 이용하여 잿빛곰팡이 병원균에 대한 항 진균성 물질을 분리하였다. 투석튜브를 이용한 분리에서 이물질이 3.5kDa 이하의 저분자물질이며 또한 내열성 물질임을 확인하였다. 이물질들은 최소한 5종의 물질로 구성되어 있으며 이중 가장 항진균성이 강한 물질을 HPLC를 이용하여 순수 분리하였으며 잿빛곰팡이병에 감염된 식물에 적용한 결과 농약과 비슷한 효과를 나타내어 환경 친화적인 항진균 물질로서 사용가능성을 확인하였다. A study of the anti-fungal properties in Korean radish was conducted using a variety of purification procedures such as Extrelut column, RP(Reverse Phase) Cl8 Column Chromatography, HPLC etc. to separate anti-fungal substances from Korean radish juices to test them against a common gray mold called Botrytis cenerea. Dialysis tube operation showed that these substances were presumably thermostable compounds with low molecular mass (less than 3.5 kDa). Differences of anti-fungal activities depending upon types of radishes used did not show any noticeable variation. The antifungals were presumably composed of more than 5 compounds. Among these, the most anti-fungal fraction was analyzed by HPLC in which one peak was obtained. Disease-affected plants were inoculated with 10mg of Extrelut fraction and results showed similar anti-fungal activity to pesticides suggesting possible usage of these substances as environmentally friendly antibiotics.

Antifungal and Plant Growth Promotion Activities of Recombinant Defensin Proteins from the Seed of Korean Radish ( Raphanus sativus L.)

Cher Won Hwang 韓國環境農學會 2009 한국환경농학회지 Vol.28 No.4

In the present study, we analyzed the defensin protein deduced from Korean radish (Raphanus sativus L.) seeds.To express the genes in E. coli, we constructed a recombinant expression vector with a defensin gene, named rKRs-AFP gene isolated from Korean radish seeds. Over expressed rKRs-AFP proteins was separated by SDS-PAGE to determine the purity, and protein concentration was determined by the Bradford method. Antifungal activity was assessed by disk assay method against the tested fungi. As a result, when 500 mL of cell culture were disrupted by sonicator, 32.5 mg total proteins were obtained. The purified protein showed a single band on SDS-PAGE with estimated molecular weight about 6 KDa, consistent with the molecular mass calculated from the deduced amino acid sequence. The purified rKRs-AFP protein showed remarkable antifungal activities against several fungi including Aspergillus niger, Botrytis cinerea causing the gray mold disease, and Candida albicans. In field tests using the purified rKRs-AFP protein, the protein showed the reducing activity of disease spot and the mitigating effect of spreading of disease like agrichemicals. The immuno-assay of rKRs-AFP protein showed that the purified protein entirely accumulated at B. cinerea cytoplasm through the hyphal septa shown by fluorescence imaging. There was no fluorescence inside the cell, when the hypha was incubated without the protein. These all results indicate that the recombinant rKRs-AFP proteins can be utilized as a potential antifungal drug to control harmful plant fungal pathogens.

한국과 미국 지역 대학생들의 환경 문제 인식에 대한 통계 분석적 비교연구

황철원 ( Cher Won Hwang ) 한국지리환경교육학회 2012 한국지리환경교육학회지 Vol.20 No.2

본 연구는 한국과 미국의 환경교육과정의 차이에 따른 환경문제 인식수준을 통계적으로 비교분석 함으로써, 한국과 미국의 환경교육과정의 차이를 이해하고, 그에 따른 효과를 파악하고자 한다. 이론적 배경으로 한국은 초등학교를 제외하고 환경과목을 독립과목으로 채택하여 실시하고 있는 반면, 미국은 대부분 중핵 교육과정(Core Curriculum)을 통한 통합적인 환경교육을 실시하고 있다. 통계분석 비교결과 첫째, 미국과 한국 모두 환경교육은 교과서를 중심으로 하는 수업을 하고 있지만, 미국의 경우 교과서 수업과 더불어 환경에 대한 호기심을 양성하는 체험학습 및 기타 영상·컴퓨터수업을 시행하고 있다. 둘째, 미국은 환경을 경험하는 기회와 그에 대한 포괄적인 배경을 제공하고, 다른 교과와 연결하여 이해시킴으로써, 미국지역 학생들은 환경수업에 대체로 만족하고 있지만, 교과서 중심의 단순한 수업을 받고 있는 한국 학생들은 환경수업에 만족하지 못하고 있다. 그 결과, 한국학생들은 환경에 대한 관심도와 참여수준이 낮은 것으로 나타났다. 따라서, 본 연구는 한국의 초·중·고 환경교육과정이 중핵 교육과정을 통한 통합적 교육으로 전환되어야 할 필요성을 시사한다. The purpose of this study is to understand the differences of environmental education course between Korean and American area, also to find out the effects on those by comparing and analyzing the situation of environmental education and environmental consciousness of both Korean and American area in the statistics. The theoretical background shows that Korean area conducts environmental education by adopting a separated education excepting the elementary school, while American area conducts integrated education through core curriculum. The results of statistic analysis are as follows. Firstly, both Korea and America conduct the environmental education through the text book, but American area conducts the environmental education with textbooks as well as experience study, video and computer classes. Secondly, the education of American area gives student high satisfaction of the environmental classes by offering various experiences, comprehensive backgrounds of environment and many chances that make students understand environment with other subjects, while Korean education did not offer satisfaction to students because the classes are mainly composed of the textbook. As a result, the interests and participations for environment are estimated to be low. Therefore, this study suggests the necessities that the environmental education of Korea needs to be converted into the intergrated education through core curriculum.

항 곰팡이 단백질 유전자 분석에 의한 국내 무 품종간 유연성에 관한 연구

황철원,Hwang, Cher-Won 한국생명과학회 2007 생명과학회지 Vol.17 No.9

국내 시판 무 (Baekwoon) 의 씨앗으로부터 항 진균 단백질들을 (RAP-l,2)분리 하였으며[12] 이들 항 진균 단백질을 MALDI-TOF실험결과, 2S storage albumin, Rs-AFP등 지하부 식물의 defensin protein과[15] 일치함을 확인하였고 이에 시판되는 7종의 각각의 무 씨앗으로부터 조 단백질과 Total RNA를 분리 하여 항 효모 (Saccharomyces cerevisiae, Candida albicans.) 및 항 곰팡이 (Botrytis cenma)에 대한 항 진균성을 실험한 결과 항 곰팡이 활성은 모든 품종에서 보였으나 항 효모 활성은2 종 (Myungsan, Baekwoon) 의 무에서만 보였 다 . 또한 기존에 알려진 항 진균 단백질 (Rs-AFP)의 유전자를 Gene Bank/EMBL 에서 획득하여 씨앗으로부터 분리한 Total RNA 에 RT-PCR 한결과, 7종 중 2종은 0.2kb 의 산물이 보이지 않았다. 이들 Ks-AFP 유전자산물을 염기서열을 분석하였으며 이 염기서열에서 얻어진 아미노산 서열을 Clustal W를 이용한 pairwise alignment 분석에 의해 국내 시판 무 의 품종간 각clone의 계통수를 분석한 결과를 보고한다. To define diversity of domestic radish, we analysis genetic relationship of anti-fungal protein genes from several domestic radish (Raphanus sativus L.) seeds. We have isolated from domestic radish (Baekwoon) anti-fungal protein named RAP[12]. In this report, we isolate RNAs and raw protein from radish seeds then, RT-PCR analysis was done with another known anti-fungal sequences of radish from Gene Bank/EMBL and anti-fun- gal, anti-yeast activity were done against Bot교tis cenerea, Saccharomyces cerevisiaeι Candida albicans with it's raw proteins. The anti-fungal activity was shown used all seeds but anti-yeast activity was shown only two seeds (Myungsan, Baekwoon). RT-PCR products (about 0.2 Kb) were not shown only two seeds. To identify the sequencing relationship of the domestic radish, we have cloned and sequenced RAP genes of the radish and analysis the sequence relationship with clustalw program. Thus we report the result that there are some different relationship between domestic radish and known other radish's anti- fungal protein[15].

흑연(GRAPHITE)의 벼 생육에 미치는 영향

황철원(Cher-Won Hwang) 한국토양비료학회 2003 한국토양비료학회지 Vol.36 No.2

포항 부추의 생화학적 기능성 효과

황철원(Cher Won Hwang),신현길(Hyun Kil Shin),도명술(Myoung Sool Do),김윤주(Yun Joo Kim),박종흠(Jong Heum Park),최영섭(Young Sub Choi),주우홍(Woo Hong Joo) 한국식품과학회 2001 한국식품과학회지 Vol.33 No.3