한국산 호도의 단백질 및 지질의 조성에 관한 연구

최청,성태수,차원섭,손규목 한국농화학회 1986 Applied Biological Chemistry (Appl Biol Chem) Vol.29 No.3

We employed gel filtration, polyacrylamide gel electrophoresis, amino acid autoanalyzer, thin layer chromatography for determining protein and lipid composition in walnut. The walnut contained 22.18% of crude protein and 64.23% of crude lipid. Glutamic acid (38.60%) was the major amino acid in soluble protein, followed by arginine and aspartic acid. The sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed 12 band in soluble protein of walnut, and collection rate of main protein fraction purified by Sephadex G-150 was 60.67%. The molecular weight for t he main protein was estimated to be 43,000. The lipid fraction obtained by silicic acid column chromatography were mainly composed of about 93.05% neutral lipid, whereas compound lipid was only 7.0% level. Among the neutral lipid by thin layer chromatography, triglyceride was 82.05%, sterol ester and free fatty acid were 3.86% and 4.80%, respectively. The predominant fatty acids of total and neutral lipids were linoleic acid (64.48∼69.98%) and oleic acid (13.89∼15.36%). The major fatty acids of triglyceride separated from neutral lipid were linolenic acid (69.98%).

2003학년도 재학생 실태조사

최청일,박자연,김주현 東亞大學校 學生生活硏究所 2004 學生硏究 Vol.32 No.-

대학입시와 시장 기능

최청일 대한사립학교장회(구 대한사립중고등학교장회) 1998 私學 Vol.83 No.-

고상법에 의한 Bradykinin 유사물의 합성 (II)

최청,이재성,배만종,윤상홍,Choi, Cheong,Lee, Jae-Sung,Bae, Man-Jong,Yoon, Sang-Hong 생화학분자생물학회 1982 한국생화학회지 Vol.15 No.4

Bradykinin(BK)의 receptor $B_1$ 상에서 nonapeptide의 구조 및 활성에 대한 연구 목적으로 bradykinin유도체 ($Leu^1$, $Leu^2$)BK, ($Leu^2$, $Leu^3$)BK, ($Ala^7$, $Ala^9$)BK 및 ($Ala^8$, $Ala^9$)BK을 고상법으로 합성하였다. coupling은 N,N'-dicyclohexylcarbodiimide로 행하였으며 HBr응액으로 cleavage한 후 peptides는 Sephadex G-25와 carboxymethyl cellulose칼럼 크로마토 그라피로 정제하였다. 이들 peptides의 순도측청은 paper chromatography, thin layer chromatography, paper electrophoresis, slab gel electrophoresis. melting point 및 아미노산 분석에 따랐다. endopeptidase인 ${\alpha}$-chymotrypsin과 trypsin, exopeptidase인 carboxypeptidase A와 leucine aminopeptidase를 사용하여 이들 peptides의 분해실험을 하였다. 즉 carboxypeptidase A와 ${\alpha}$-chymotrypsin은 이들을 빠르게 분해하였고 trypsin은 느리게 분해 하였으나, ($Ala^7$, $Ala^9$) 및 ($Ala_8$, $Ala^9$)BK은 N-말단에 모두 proline의 imino와 결합되었기 때문에 leucine aminopeptidase의 작응을 받지 않았고 ($Leu^1$, $Leu^2$)BK은 1번 위치에 arginine과 같은 방향족 아미노산이 치환되지 않았으므로 trypsin의 작응을 받지 않았다. We have synthesized, by solid phase method, a series of 4 analogues of the nonapeptide, bradykinin(BK) in order to conduct a structure-activity study of these peptides on the newly discovered bradykinin $B_1$ receptor. Coupling were performed by dicyclohexylcarbodimide. After cleavage with liquid HBr, peptides were purified by gel fitration on Sephadex G-25 and ion-exchange chromatography on carboxymethyl cellulose. The purity of the nonapeptide was then checked by paper chromatography, thin layer chromatography, paper electrophresis slab gel electrophoresis, melting point and amino acid analysis. ($Leu^1$, $Leu^2$), ($Leu^2$, $Leu^3$), ($Leu^7$, $Leu^9$) and ($Leu^8$, $Leu^9$) analogues of bradykinin were incubated in vitro with endopeptidase ($\alpha$-chymotrypsin, trypsin) and exopeptidase (carboxypeptidase A, leucine aminopeptidase) in order to study the degradation patterns of peptides. ($Leu^1$, $Leu^2$), ($Leu^2$, $Leu^3$), ($Leu^7$, $Leu^9$) and ($Leu^8$, $Leu^9$) analogues of bradykinin were rapidly degraded by $\alpha$-chymotrypsin and carboxypeptidase A, while the degradation by trypsin was slow. But ($Ala^7$, $Ala^9$) BK and ($Ala^8$, $Ala^9$)BK contain imino peptide bond from proline at N-terminal and therefore they were not attacked by leucine aminopeptidase.

고상법에 의한 Angiotensin Ⅱ 유사물의 합성(제2보)

崔淸 嶺南大學校附設 基礎科學硏究所 1982 基礎科學硏究 Vol.2 No.-

Synthesis of (5-Gly), (6-Gly) and (8-Gly) analogues of angiotensin Ⅱ by solid phase method using an improved reaction vessel was carried out. I employed gel chromatography, paper chromatograpy, thin layer chromatography, paper electrophoresis, salb-gel electrophoresis and amino acid analysis for purification and for determining the purity of synthesized peptides. (5-Gly), (6-Gly), (7-Gly) and (8-Gly) analogues of angiotensin Ⅱ were incubated in vitro with endopeptidase (α-chymotrypsin, trypsin) and exopeptidase (carboxypeptidase A, leucineamineaminopeptidase) in order to study the degradation patterns of the peptides. (5-Gly), (6-Gly), (7-Gly) and (8-Gly) analogues of angiotensin Ⅱ were rapidly degradated by α-chymotrypsin, trypsin, and carboxypeptidase A while the degradation by leucineaminopeptidase is slow. (7-Gly) angiotensin Ⅱ contain imino position and therefore it was not attacked by leucineaminopeptidase.

地方文化와 地方大學의 役割과 機能

최청일,설기문,박영태,이광원 東亞大學校 學生生活硏究所 1992 學生硏究 Vol.20 No.-

A local culture ought to be established by local citizens and existed for the sake of local citizens, at the same time a local culture should be cultures developing, prospering the locality. That is why a local culture must be separate from centralizm. On this account it is necessary that local inhabitants should create their own cultures in their own way. Up to now, however, a central government has controlled local cultures, so we find it very difficult for the inhabitants to form creative cultures for their own locality. Therefore universities, colleges, and community colleges where are located in the locality are now asked to play initiative roles to produce local cultures. There are factors to make allowances for advancing local cultures; that is, the qualities, a cultural space, a cultural organization, cultural events, maintenance and transmission. The functions of higher educaion are instruction, research, service, these general functions of higher education and the above factors are indispensable to the development and creation of local cultures. In this paper we mean to investigate the functions and roles of higher education in Pusan area for the prosperity of local cultures. The followings specify the functions and roles of higher education for the advancement of local cultures. 1. Higher education has the responsibility of creating and maintaining of the qualities of local cultures corresponding to the area. Pusan is surrounded with rivers, seas, mountains and has plentiful of traditional inheritances. In spite of these environments Pusan is regarded as a marine city, Pusan has not only activities to maintain qualities as a marine city but also contains activities to keep pace with cultural qualities as a riverside city, a mountainous city including historical inheritances. 2. Higher education tries to play an important role to search for programs to utilize the existing cultural space and to devise new cultural space. In case local qualities in Pusan are divided into riverside, mountainous, marine, and traditional, Dong-A University and Busan Junior College are situated at Nak-dong River, so they had better devise cultural acticities in connection with riverside cultures. Busan National University, Dong- Ui University, Busan Women's University, Busan Teachers'College are surrounded with mountanous areas, therefore they had better plan cultural programs to devise activities connected with mountainous cultures. Korean Marine University, Gyongsong University, Susan University, Goshin University are surrounded with seas, so they had better devise programs to make use of activities related to marine cultures. 3. Higher education should provide local citizens with the facilities for cultural space. 4. Higher education should educate persons who keep and create local cultures. 5. Higher education should have close ties with cultural organizations in the locality to activitize and study the programs, and supply them with the materials. 6. Higher education should make their resource personnel take part in the activities of the cultural organizations and contribute to the development of the cultural organizations. 7. Higher education should get their own members to participate in the cultural events and to help to activitize the events. 8. Higher education should develop cultural event corresponding to local qualities and devide them into weak points and strong points to activitize the program. 9. Higher education should participate in the cultural events and manage the events. 10. Higher education should have research activities to keep the existing local cultures and to develop new cultural patterns. 11. Higher education should have their own members educate the inhabitants to transmit local cultures and try to practise examplary cultural patterns.

사학재정의 정책방향

최청일,고영진 東亞大學校 學生生活硏究所 1995 學生硏究 Vol.17 No.-

대학재정, 어떻게 운영(관리, 통제)할 것인가?

최청일 한국교육행정학회 1992 敎育行政學硏究 Vol.9 No.2

敎育投資의 意思決定 基準分析

崔淸一,天九泰 東亞大學校 學生生活硏究所 1992 學生硏究 Vol.20 No.-

From the perspective of economics, educational decision can be thought of as producing human capital;that is, as embodying knowledge in students that has subsequent market value through increased labor market skills, broadly conceived, upon entry into the work force. The purpose of ths paper is to discuss rational decision criteria for human capital investment. The educational decision criteria can be divided into micro approaches and macro approaches. The micro approaches consist of rates of return, internal rate of return, net present value, benefit-cost ratio, cost-effectiveness. The macro approaches involve residual growth, manpower planning, social demand approach. Rate of return is that percentage rate of return that discounts the stream of earnings expected by the student over his life cycle back to its present value and equate them to the total educational costs compounded forward to the date of graduation. The internal rate of return is that discount rate which equates the present values of benefits and costs or the same things as sayings it is that discount rate at which the difference between the discounted value of benefits and the discounted value of costs is equal to zero. The net present value is the difference between the present values of benifits and of costs. We had better select all projets where the present value of benefits exceeds the present value of costs. The benefit-cost ratio means that the ratio of the present value of benefits to the present value of costs exceeds unity. A project will be worthwhile whenever B_0/C_0>1. Cost-effetiveness analysis is a technique for measuring the relationship between the total inputs, or costs, of a project or activity, and its outputs or objectives. This method may be employed to make choice of a project from among the alternatives, where programe effects are not valued in money terms and where imputing values will be difficult. The sources of economic growth are not only physical capital and labor but also education, health, on-the-job training. The contribution of these latter groups of factors to econonic growth is that part of the growth which can't be explained by increases in physical capital and labor inputs. The unexplained part of growth is generally termed residual growth. Manpower planning attempts to relate manpower needs of the economy to manpower supply through education and training when planning economic development. Social demand approach refers to the demand for education emerging from the needs and aspirations of individual persons as contrasted the educational demand based on the personnel requirements of society. The above criteria should be put educational decision in human capital investment into use.

固相法에 의한 Angiotensin II 유기물의 合成 (III)

崔淸 嶺南大學校附設 基礎科學硏究所 1986 基礎科學硏究 Vol.6 No.-

Synthesis of (Ala¹, Ala²), (Ala¹, Ala³) and (Ala¹, Ala⁴) analogues of angiotensin Ⅱ by solid phase method using an improved reaction vessel was carried out. The yield average 62.8∼66.5%. Homogeneity and purity of the in three solvent systems syntheized peptides were established with paper chromatography, paper electrophoresis, amino acid analysis and melting point apparatus. The pressure activity was gradually increased with the increase in the chain length from the N-terminal and of (Ala¹, Ala²) angiotensin Ⅱ, however the maximum value was within 30%. (Ala¹, Ala²), (Ala¹, Ala³)and (Ala¹, Ala⁴) analogues of angiotensin Ⅱ were incubated in vitro with α-chymotrypsin and leucineaminopeptidase in order to study of degradation patterns on the peptides. (Ala¹, Ala²), and (Ala¹, Ala³) analogues of angiotensin Ⅱ were rapidly degradated α-chymotrypsin and leucineaminopeptidase. (Ala¹, Ala⁴)angiotensin Ⅱcontain imine group position and therefore it was not attacked by leucineaminopeptidase.