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赤血球膜의 Cholesterol含量 變動이 補體溶血에 미치는 效果
孫在卿,金麗姬,鄭泰浩 慶北大學校 醫科大學 1985 慶北醫大誌 Vol.26 No.3
세포막의 지질변동은 그세포를 표적으로 하는 세포상해 반응에 큰 영향을 줄수있다는 보고에 따라 본연구에서는 사람 정상적혈구막의 cholesterol 함량을 변동시켜 mouse antihuman hemolysin과 rabbit complement에 의한 용혈 정도를 측정하였다. 적혈구를 cholesterol로 처리한 cholesterol-enriched군의 적혈구막의 C/P molar ratio는 1.52±0.11으로 대조군의 0.82±0.08에 비해 유의성 있게 증가된 반면, 적혈구를 phosphatidylcholine으로 처리한 cholesterol-depleted군의 혈구막의 C/P molar ratio는 0.58±0.05으로 대조군에 비해 유의성 있게 감소되었다. 한편 mouse antihuman hemolysin과 가토보체를 적혈구와 반응 시켰을때 적혈구를 50% 용혈시키는 보체농도의 평균치는 cholesterol-enriched 군에서는 0.047±0.048㎖로 대조군의 0.09±0.047㎖보다 유의성있게 낮았으며, cholesterol-depleted 군에서는 0.117±0.055㎖로 대조군보다 유의성있는 높은 치를 나타내었다. 즉 적혈구막의 cholesterol함량이 높을수록 보체용혈에 대한 적혈구의 감수성이 높음을 나타내었다. The membrane lipid fluidity of normal human erythrocytes was modified by enrichment and depletion in cholesterol, and the expression of surface antigens was assayed by complement hemolysis test. Human erythrocytes, sensitized by specific mouse antihuman hemolysin, showed a significant increase in susceptibility to complement lysis after rigidification of the membrane lipid layer by incorporation of cholesterol. Conversely, fluidization of the membrane lipid layer by treatment with phosphatidylcholine for cholesterol depletion displayed increased tolerance to the complement lysis.
손재경,이시헌 대한의진균학회 2001 대한의진균학회지 Vol.6 No.2
Background: Onychomycosis is a common disease and has a tendency to increase in its incidence. Although there have been many reports about onychomycosis, very few studies about onychomycosis in the elderly have yet been conducted. Objective: The purpose of this study was to investigate the clinical and mycological features of onychomycosis in the elderly, and to evaluate the concept and attitude of the elder patients towards onychomycosis, which would affect the treatment and further infections of onychomycosis. Method: Sixty eight onychomycosis patients over 60 years old were examined clinically and surveyed by questionnaire to evaluate their concern about the onychomycosis. All the patients were confined by potassium hydroxide (KOH) examinations and underwent cultures on the both cornmeal potato agar with and without cycloheximide. An additional culture was done a week later when the intial culture showed contamination or no growth. Results: Among 648 patients with onychomycosis, 68 patients (10.5%) were over 60 years old. Fifty(73.5%) were female and 18 (26.5%) ware male. Twenty six (38.2%) had systemic illness. The average duration of onychomycosis was 13 years and the average number of onychomycosis involving nails was 4.7. Sixty three patients (92.6%) had toe nail onychomycosis. Sixty six patients (97.1%) showed distal subungal onychomycosis. Trichophyton rubrun was the main causative organism in 38 patients (55.9%). Forty one patients (60.3%) discontinued the medication in 4 weeks, and 11 (16.2) were treated over 12 weeks. From the questionnaire, only 10 patients reported previous treatment far this problem, but with duration of treatment no longer than one month. Fifty three patients had mere understanding atypical alternation in their nails before the visit. The main reasons of the discontinuing the medication were the cost of the treatment, physical discomfort due to age, and extended duration of the therapy. Conclusion: From this study, we noticed that the elder onychomycosis patients could be long time carriers of fungi because of their lack of the knowledge of onychomycosis and insufficient treatment. Therefore, it is necessary to deucate and support to treat the elder patients effectively. [Kor J Med Mycol 6(2): 77-83]
손재경(Jae Kyung Son),이동섭(Tong Sup Lee),양한섭(Han Seob Yang) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.5
To understand the role of shelf sediment in phosphorus biogeochemical cycle, we carried out sequential sediment extraction (SEDEX) of P and porewater analysis on 14 core samples collected in the South Sea of Korea. SEDEX classified P-pools into 5 phases and results are grouped into two cateories: reactive P (loosely sorbed-P and Fe bound-P) and refractory P (detrital inorganic-P, authigenic mineral-P and organic-P). Total P concentrations are decreased with sediment depth in all samples as a result of dissolution to porewater. Reactive P comprises about 20∼50% of total P, and iron bound-P is the major form consisting 70∼80% of reactive P-pool. Iron bound-P decreases sharply with depth. Depth profiles of dissolved P concentration in porewater show mirror image of iron bound-P, revealing the role of FeOOH as a regulator of reactive P supply to overlying water culumn. Authigenic mineral-P consists less than 5% of total P, thus removal of reactive P by converting into refractory P seems inefficient in shelf sediment. This implies that continental shelf sediment sequesters P temporarily rather than permanently. Results show local variation. Nakdong estuary receiving large amount of terrigenous input shows the highest concentration of total P and reactive P. Here iron oxyhydroxides at the surface sediment control the water column flux of P from sediment. Although total P content at the surface is comparable (500∼600 ㎍ g^(-1)) between the South Sea and East China Sea, the former contains more iron bound-P and less derital inorganic-P than the latter. Reasons for the difference seem due in part to particle texture, and to biological productivity which depends roughly on the distance from land.
얇은막대 배치작업에 대한 N-R 과 EKF 방법을 이용하여 개발한 로봇비젼 제어알고리즘의 평가
손재경(Jae Kyung Son),장완식(Wan Shik Jang),홍성문(Sung Mun Hong) 대한기계학회 2013 大韓機械學會論文集A Vol.37 No.4
실제 산업현장에서 비젼 시스템을 적용하기에는 로봇 비젼 제어알고리즘의 기구학모델의 정확도, 로봇이 움직이는 동안 카메라 초점거리와 방위에 대한 보정, 3 차원 물리적 좌표에서 2 차원 카메라 좌표로의 매핑에 대한 이해 등 해결해야 할 많은 문제점들이 있다. 본 논문에 제안된 비젼 시스템 모델은 카메라와 로봇 사이의 상대적인 위치가 알려지지 않아도 제어가 가능하고, 카메라 보정 문제를 해결하기 위해 6 개의 카메라 매개변수를 가지는 비젼 시스템 모델을 제시하였으며, 이를 이용하여 로봇 비젼 제어알고리즘 개발에 N-R 방법과 EKF 방법을 적용하였다. 최종적으로 N-R 과 EKF 방법에 의하여 개발된 로봇 비젼 제어 알고리즘의 위치 정밀도와 데이터 처리 시간을 얇은 막대 배치작업을 수행하여 비교하였다. Many problems need to be solved before vision systems can actually be applied in industry, such as the precision of the kinematics model of the robot control algorithm based on visual information, active compensation of the camera’s focal length and orientation during the movement of the robot, and understanding the mapping of the physical 3-D space into 2-D camera coordinates. An algorithm is proposed to enable robot to move actively even if the relative positions between the camera and the robot is unknown. To solve the correction problem, this study proposes vision system model with six camera parameters. To develop the robot vision control algorithm, the N-R and EKF methods are applied to the vision system model. Finally, the position accuracy and processing time of the two algorithms developed based based on the EKF and the N-R methods are compared experimentally by making the robot perform slender bar placement task.