담낭암의 발생에서 담췌관합류이상의 역할 규명과 암화과정에 관한 연구

한기준,송시영,정재복,이세준,김호근,강진경 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.4

담낭암 발생에서 담췌관합류이상의 역할 규명과 암화과정에 관한 연구

강진경,한기준,이세준,송시영,정재복,김호근 대한소화기학회 1998 대한소화기학회지 Vol.32 No.4

Baekground/Aims: To clarify the careinogenesis of gallbladder cancer (GBC) in patients with anomalous pancreaticobiliary ductal union (APBDU), we investigated the expression of mutated p53 protein and c-erbB2, the cell proliferation indices of Ki-67 and the histopathologic changes in gallbladder carcinoma and noncaneeraus mucosa which are associated with APBDU. Methods: In the clinical review, we analysed 62 patients (27 patients of GBC with APBDU and 35 patients of GBC without APBDU) who were diagnosed in Sevrance Hospital from January, 1992 to December, 1994. Immunohistochemical staining and histologic examination were performed on the specimens obtained from surgically resected gallbladders from the patients. For comparative study, the specimens were grogped as follows: 5 cases of carcinoma with APBDU, 4 eases of noneancerous mucosa with APBDU associated with GBC, 11 cases of noncancerous mucosa with APBDU not associated with GBC, 19 cases of carcinoma without APBDU and 7 cases of chronic inflammatory mucosa of gallbladder stones as controls. Results, GBC developed in 33.3% of patients with APBDU (especially, 66.7% of PC type) and showed no gallbladder stones. The frequency of papillary carcinoma was higher and depth of invasion was less in GBC with APBDU than in GBC without APBDU. Among 15 cases of noncancerous mucosa with APBDU, isolated dysplasia and adenomyomatous hyperplasia were noted in 10 cases and 4 cases, respectively. In one case of APBDU with GBC, the expression of p53 protein was noted in the focal area of low grade dysplasia. Cell proliferation indices of Ki-67 were stepwise increased with the progression of histologic findings from inflammation, papiUary hyperplasia, metaplasia and adenomyomatous hyperplasia to dysplasia. Particularly, remarkable increase was observed in adenomyomatous hyperplasia and dysplasia. Conclusions: The high incidence of isolated dysplasia and adenomyoma accompanying with increased cell proliferative indices seems to be closely related to th carcinogenesis of GBC in patients with APBDU. Additionally, the mutation of p53 may contribute to early event of carcinogenesis in some patients with APBDU.

한타바이러스와 그 숙주동물

송기준 대한감염학회 1996 감염 Vol.28 No.2

1994∼1995년 한국에서 발생한 가을철 열성질환에 대한 혈청역학적 연구

송기준,송진원,백락주,김상현,김세연,이용주 대한감염학회 1998 감염 Vol.30 No.4

목 적 : 최근 우리나라에서 발생했던 금성 열성질환의 전반적인 혈청역학적 양상의 분석을 위해 1994년부터 1995년까지 고려대학교 의과대학 미생물학교실, 바이러스병연구소에 급성 열성질환으로 검사가 의뢰된 환자혈청 5330건을 쯔쯔가무시병, 발진열, 신증후출혈열, 라임병 및 랩토스피라증에 대한 항체를 조사하였다. 방 법 : 1994년부터 1995년까지 2년간 고려대학교 의과대학 미생물학교실, 바이러스병 연구소에 검사가 의뢰된 환자혈청을 주 대상으로 간접면역형광항체법 검사로 쯔쯔가무시병, 발진열, 신증후출혈열 및 라임병에 대한 항체양성 유무를 검색하고 macroscopic agglutination법으로 렙토스피라증에 대한 항체를 조사하였다. 결 과 : 열성질환 환자 혈청 5330건을 대상으로 신증후출혈열, 쯔쯔가무시병, 발진열 및 라임병에 대한 항체검사 결과 쯔쯔가무시 항체양성자가 12% (640/5330)로 항체양성자 중 여자가 60%로 60대가 30% (192명)로 가장 많았다. 월별 분포에서는 대부분의 양성자 (89%)가 가을철에 집중되어 있었다. Richettsia, typhi에 대한 항체를 양성률을 보였다. 발진열 항체 양성자 중 남자가 60% (106/178)로 여자보다 많았으며, 모든 연령 층에서 발견되었으나 40∼50대가 57% (107명)로 가장 많았다. 대부분이 양성자 (44%)가 늦가을 (10∼12월)에 발생하였다. Macroscopic agglutination법에 의한 렙토스피라에 대한 항체률을 조사한 결과 1% (55/5330)의 항체 양성률을 보였으며 연도별로는 1994년 0.4% (11/2799), 1995년 1.7% (44/2531)로 1995년에 더 많은 항체 양성자가 발생하였다. 총 55건의 렙토스피라 항체양성자 중 남자가 71% 였으며, 연령별 분포에서는 60대가 47%로 가장 많았으며 20대 이상의 성인에서만 항체가 발견되었다. 대부분의 항체양성자 (78%)가 8∼10월에 집중되었다. 라임병은 총 5330건 중 항체양성자는 없었다. 결 론 : 1994∼1995년 우리나라에서 발생한 발열성질환환자 중 쯔쯔가무시병, 발진열, 신증후출혈열 및 렙토스피라증 환자가 전체의 29%였으며 항체 양성자수로는 신증후출혈열,쯔쯔가무시병,발진열,렙토스피라증 순이였다. 따라서 위의 4가지 질환이 우리나라에서 특히 가을철에 발생하는 급성열성질환의 주원이으로 생각되나 새로운 질병이나 신종 병원체에 의한 동일한 질환이 존재할 가능성이 매우 높으므로 지속적인 혈청역학적 조사가 수행되어야 할 것이다. Background :The principal acute febrile diseases reported in autumn, Korea, have been hemorrhagic fever with renal syndrome (HFRS), scrub typhus, murine typhus and leptospirosis. Methods : To evaluat the seroepidemiologic patterns of acute febrile illness in Korea, sera were collected from 5330 patients from 1994 to 1995, and examined for antibodies against Hantaan virus, Orientia tsutsugamushi, Rickettsia typhi, and Borrelia burgdorferi by indirect immunofluorescent antibidy technique (IFA) and by macroscopic agglutination test for Leptospira interogans. Results : Of 640 seropositive cases against O. tsutsugamushi, 60% were female; age group of the seventh decade occupied 30%; 89% were reported during October and November. By IFA test, the seropositive rate of murine typhus was 3.5% (18/5330) with high incidence rate (44%) in the period from October to December, and males in their forties and fifties were mostly affected. Cases seropositive for leptospirosis consisted 1% of 5330 acute febrile illness cases. Of 55 seropositive cases, 71% were male; 71% were in their sixties; 78% occurred during the period from August to October to January and males in the thirties were mostly infected. No seropositive case of Lyme disease was present. Conclusion : The above results indicate that hantavirus, O. tsutsugamushi, R. typhi, and L. interogans were the main causative agents of acute febrile diseases during autumn season in Korea.

皮膚科 外來 癩患者의 動態

宋浚榮,朴義守,徐舜鳳,徐基錫,河龍馬,孫在卿,金基洪,金蘭姬 大韓癩學會 1981 癩學 會誌 Vol.14 No.1

Hydroxy Apatite가 피복된 치과매식체의 주위골조직 반응에 관한 조직학적 연구

송준기,허성주,조인호 단국대학교 치의학연구소 1991 논문집 Vol.3 No.-

Even if we can recover the function of mastication, pronunciation and esthetic using the fixed or removable prosthesis in the loss of teeth or hard tissue in the oral cavity, we use several kinds of implants in order to solve the problem which can be occured when we can't install the denture because of excessive bone resorption or psychlogical affairs. At present Titanium implant plays a major role in this field and has osseointegrated. And the study on the modern material is going on, that result in developing and using the implant which is coated with HA, bone induced material. In this, I studied histologically the change of the bone tissue which is occured when three kinds of HA coated implants. Such as Integral, Sustain, Biovent implanted into mandible of dog and got a 8-weeks healing period. I got the conclusion as follows : 1. Most of the implant which is covered with serveral kinds of HA coating implant in bone affter 8 weeks being implanted and has osseointegrated, partially converted into the connective tissue. 2. Biovent formed the connective tissue in the perforated area of inferior alveolar canal and has osseointegrated.

조갑진균증의 치료에서 터비나핀과 이트라코나졸의 이중맹검 비교연구 : 공동연구

김기홍,최종수,송준영,김상원,김수찬,안성구,성열오,서순봉 대한화학요법학회 1995 대한화학요법학회지 Vol.13 No.2

저자들은 새로 개발된 alyllamine계 항진균제인 터비나핀의 발톱의 조갑백서에서 치료 효과, 내약성 및 부작용 등을 평가하기 위해 triazole계의 이트라코나졸과 이중 맹검으로 비교 조사하였다. 치료방법은 터비나핀은 250㎎/days로 12주간, 이트라코나졸은 100㎎/day로 124주간 복용하고 양군 모두 치료 시작 후 36주까지 추적 관찰하였다. 조사방법은 조갑진균증 환자에 대해 치료에 대해 설명후 동의서를 받고 장기간 치료가 부적합한 경우를 제외한 149명(터비나핀군 74명, 이트라코나졸군 75명)이 치료를 받았으며 이중 25명이 탈락하고 13명이 부작용등으로 치료를 중지하여 111명(터비나핀군 51명, 이트라코나볼군 60명)이 치료를 완료하였다. 1. 임상적인 소견은 점진적으로 호전되었으며, 치료 시작후 36주에는 병변조갑의 감소 비율로 볼때 터비나핀군은 51명중 완전치료가 24(47.0%), 76%이상 호전이 18명(35.3%), 51%~75% 호전이 6명(11.8%), 26~50% 호전이 2명 (3.9%), 25% 이하의 호전을 보인 예는 1명(2.0%)였다. 이트라코나졸군은 60명중 완전치료가 27(45.0%), 76% 이상 호전이 14명(23.3%), 51%~75%호전이 9명(15.0%), 26~50% 호전이 2명(3.9%), 25% 이하의 호전을 보인 예는 1명(2.0%)였다. 이트라코나졸군은 60명중 완전치료가 27(45.0%), 76% 이상 호전이 14명(23.3%), 51%~75%호전이 9명(15.0%), 26~50% 호전이 7명 (11.7%), 25% 이하의 호전을 보인예는 3(5%)였다. 2. KOH도말 검사에서 음성 소견을 보인 비율은 터비나핀은 24주 후 50예(98.0%)였으며, 36주후에는 모두 음성을 나타내었고, 이트라코나졸은 치료 24주 후 51예(85.0%)였고, 36주후에는 59예(98.3%)에서 음성을 나타내었다. 진균배양 검사에서는 치료 12주 후에 터비나핀군은 51예중 44예에서 (86.3%), 이트라코나졸군은 60예중 52예에서 각각 음성이었으며(86.7%), 치료 36주 후에는 양군 모두에서 음성이었다. 3. 내약성은 양군 모두 시간이 지나면서 증가하였으며, 터비나핀은 moderate이상이 98~·00%였고, 이트라코나졸은 moderate이상이 96.7~100%였다. 4. 부작용은 위장관 증상이 가장 많았고 터비나핀은 23명, 이트라코나졸은 37명이었고, 2명, 3명에서 각각 일시적인 간효소치의 상승이 있었다. 이상의 결과로 보아 조갑진균증의 치료에 터비나핀 1일 250㎎씩 12주간의 경구투여는 치료율, 안정성 및 부작용에서, 추천할 만한 치료법이라 생각된다. In this randomized double-blind trial on multicenter study, the efficacy of the new antifungal agent, terbinafine(Lamlsil) was compared with the triazole antifungal agent, itraconazole, in the treatment of patients with onychomychosis. One hundred and forty nine patients(74 terbinafine, 75 itraconazole) with onychomlychosis were enrolled. Twenty five patients were lost to follow up and 13 reported adverse reaction with premature discontinuation of therapy. And 111 patients were eligible for follow-up until 36 weeks after starting the treatment. Fifty one patients received Lamisil(terbinafine) 250mg daily for twelve weeks (terbinafine group) and 50 received itraconazole 100mg daily for 24 weeks (itraconazole group). They were checked clinical symptoms(onycholysis, subungual hyperkeratosis, paronychyal inflammation, color change, brittleness, ridging, normal length of nails) and mycological improvement with KOH wet mount and culture during the study. Clinical improvements were slow but progressive. In terbinafine group of 5t patients, 24 patients(47.0%) were completely cleared and 18 patients(35.3%) were improved more than 76%, and 6 patients(11.8%) were improved in 53-75%. In itraconazole group of 60 patients, 27 patients(45.0% ) were completely cleared and 14 patients(23-3%) were improved more than 76%, and 9 patients(150%) were improved in 51-75% . In mycologic study, cure rate of the terbinafine group showed all negative at 36 week after the treatment while ittraconazole group showed all negative in culture but negative KOH in 98.3%. Adverse reactions and tolerability of both the drugs were good and net different significantly. All these findings suggest that the efficacy of Lamisil(terbinafine) 250mg daily for 12 weeks in the treatment of onychomycosis was similar to that of itraconazole 100mg daily for 24 weeks.

Linear Alkylbenzene Sulfonates 분해세균의 생태학적 연구 : 수영천과 온천천을 중심으로

김전기,박송희,이상준 부산대학교 환경문제 연구소 1987 環境硏究報 Vol.5 No.-

부산시 수영만으로 유입되는 수영천과 온천천의 4개지점에서 1985년 5월부터 1986년 4월까지 매월 1회 채수한 시료로부터 분해세균을 분리동정하여 그 생태학적 특성에 관해 조사한 결과는 다음과 같다. 1. 수온은 평균 18.2±7.72℃ 이었으며, 그 범위는 4.5-30.0℃이었다. 월별 수온변화는 2월이 가장 낮고 8월이 가장 높았다. 2. pH는 평균 6.8±0.26 이었으며, 그 범위는 6.4-7.2이었다. 여름철은 약알카리성, 겨울철은 약산성을 나타내었다. 3. LAS 농도는 평균 4.8±2.11ppm 이었으며, 그 범위는 1-9 ppm이었다. 지점별로는 온천교 6.0±1.88ppm, 이섭교 5.9±2.30ppm, 원동교 4.0±1.33ppm, 수영교 3.5±1.51ppm의 순이었다. 4. LAS 분해균수의 변화는 LAS 농도보다는 오히려 계절적인 영향을 많이 받는것으로 나타났다. 5. 총균수에 대한 LAS 분해균의 점유율은 봄철이 0.4%로 가장 높았으며, 지점별로는 온천교 부근이 가장 높았고 수영교 부근이 가장 낮았다. 6. 분리된 LAS분해균주를 분류동정한 결과 총 15속으로 대부분 그람음성세균이었으며, Listeria속만이 그람양성세균으로 나타났다. 7. 전 지점에서 년평균 10% 이상의 출현빈도를 보인 LAS 분해세균은 Alcaligenes속, Klebsiella속, Pasteurella속이었다. 8. Pasteurella 속이 전지점에서 우점종으로 나타났으며, 온천교 부근과 이섭교 부근에서는 Klebsiella속, 원동교와 수영교 부근에서는 Alcaligenes 속이 준우점종으로 나타났다. Lenear alkylbenzene sulfonates(LAS) degrading bacteria were isolated from the Oncheon and the Sooyoung streams in Pusan area. Samples were monthly taken from the surface waters at four designated sites from May, 1985 to April, 1986. The isolates strains were identified and ecological studies on these identified strains were investigated. The results obtained were as follows: 1) Average water temperature was 18.2±7.72℃ with range of 4.5 to 30.0℃. Water temperature was the lowest in February and the highest in August. 2) The mean value of pH was 6.8±0.26 with range of 6.4 to 7.2. The pH value showed slight alkalinity in the summer and slight acidity in the winter. 3) The mean concentration of LAS was 4.8±2.11ppm with range of 1 to 9 ppm. The highest concentration of LAS was found in the water sample of the Oncheon bridge area(6.0±1.88ppm) followed by the Iseup bridge (5.9±2.30ppm), the Weondong bridge(4.0±1.33ppm), and the Sooyoung bridge area(3.5±1.51ppm). 4) Number of the LAS degrading bacteria was effected by the seasonal change rather than LAS concentration. 5) The ratio of the LAS degrading bacteria to the total cells was the highest as 0.4% in spring. The highest and lowest ratio was at the Oncheon and the Sooyoung bridge area respectively. 6) All isolated strains were characteristically Gram-negative bacteria except only Listeria genus. 7) The appearance frequency of Alcaligenes, Klebsiella, and Pasteurella was showed more than 10% at all sites. 8) The dominant strains of LAS degrading bacteria were genus Pasteurella at all sites. The subdominants were genus Klebsiella at the Oncheon and the Iseup bridge area, and genus Alcaligenes at the Weondong and the Sooyoung bridge area.

5-fluorouracil이 결막fibronectin에 미치는 영향 : Immune Electron Microscopic Findings 배양하 면역전자현미경소견

최준규,송기영,고명규 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.1

The success of the filtering in glaucoma is dependent n the patency of the outflow pathway of the aqueous humor from the anterior chamber to the filtering bleb. This is influenced by many factors such as surgical technique, physiologic and pharmacologic factors. Most of the failure of the filtering bleb formation has resulted from the scar formation of teh bleb. This is mainly derived from the fibroblast proliferation which blocks the patent bleb site. The purpose of this study is to investigate the effect of the 5-FU on the extracellular matrix. This involves mainly fibronectin excreted from in vitro, by means of the immunocytochemical stain, at the ultrastructural level. The rabbit conjunctival fibroblast and blood vessel inteh conjunctival stroma were excised and placed in a culture dish containing D-MEM and fetal calf seru. After 2nd week cultivation in 5% CO₂, and 95% air humidified at 37℃, the 5-FU(0.5mg/L) was added. At the 4th week,the culture was terminated for immunocytochemical electron microscopy. The tissue was stained with antifibronectin antibody, followed by incubation with biotin-conjugated goat anti-rabbit IgG. After labelled with HRP-labelled avidin, the tissue was stained with Diaminobenzidine. The fibroblast in the control group showed an indented nucleus with prominent nucleoli, and numerous cytoplasmic inclusions within the cytoplasm. In addition, the cytoplasmic surfacr showed numerous villous processes. However, the plasma membrane was ruptured, and the microorganelles underwent degenerative changes in the fibroblast treated with the 5-FU. The immunoelectorn microscopy revealed an absent or weak reaction product on the vascular wall and plasma membrane of the fibroblast in the 5-FU treated specimen. In short, the basement membrane of the 5-FU added cultured cel revealed a reaction product that was faint when compared with the strong reaction product in the control group. These results suggest, that 5-FU, in a cultured fibroblast and vessel of the conjunctiva, strongly suppresses the excretion of the extracellular matrix, particularly fibronectin. These results suggest, that the application of 5-FU in a filtering operation may be useful for the success of glaucoma surgery, by preventing the secretion of the extracellular matrix, particularly fibronectin.

공기압 실린더의 위치제어기 선정에 관한 연구

김일수,김기우,정영재,손준식,송창재,박주석 한국공작기계학회 1999 한국생산제조학회지 Vol.8 No.6

This paper concentrates on the position control of a pneumatic cylinder under parameter variation. A closed-loop control is proposed to design the different controllers(P, PI, PID, PD controller) in order to choose the best controller based on the fast and accurate control of the system. It is shown that the control algorithm is robust and effective in attaining the fast and accurate position control of system under time-dependent parameter variation. Experimental results showed that PD controller law is effective to obtain the fast response and to increase the stability of the system. The method is a useful control algorithm which always automatically adjusts the position control in accordance with the error, using carrier wave of triangle type regardless of changes on the operating condition and physical differences between components.