Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury

Yu Zhenjun,Li Yuhan,Shao Shuai,Guo Beichen,Zhang Mengxia,Zheng Lina,Zhang Kun,Zhou Feng,Zhang Li,Chen Chiyi,Jiang Wentao,Hong Wei,Han Tao 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Some long noncoding RNAs (lncRNAs), which harbor microRNAs in their gene sequence and are also known as microRNA host gene derived lncRNAs (lnc-MIRHGs), play a dominant role alongside miRNAs, or both perform biological functions synergistically or independently. However, only a small number of lnc-MIRHGs have been identified. Here, multiple liver injury datasets were analyzed to screen and identify the target lncRNA Mir122hg. Mir122hg was mainly enriched in liver tissues with human-mouse homology. In both CCl4-induced acute liver injury and Dgal/LPS-induced fulminant liver failure in mice, Mir122hg was sharply downregulated at the early stage, while a subsequent significant increase was only found in the CCl4 group with liver recovery. Overexpression and silencing assays confirmed that Mir122hg played a protective role in acute injury by promoting hepatocyte proliferation in vivo and in vitro. Consistent with the results of gene enrichment analysis, Mir122hg binding to C/EBPα affected its transcriptional repression, promoted gene transcription of downstream chemokines, Cxcl2, Cxcl3, and Cxcl5, and exerted pro-proliferative effects on hepatocytes through activation of the AKT/GSK-3β/p27 signaling pathway by CXC/CXCR2 complexes. This study identifies a novel lncRNA with protective effects in acute liver injury and demonstrates that the binding of Mir122hg-C/EBPα promotes hepatocyte proliferation via upregulation of CXC chemokine and activation of AKT signaling.

The Adsorption and Desorption of NH₃ on Rutile TiO₂(110)-1×1 Surfaces

김보성,Zhenjun Li,Bruce D. Kay,Zdenek Dohnalek,김유권 한국진공학회 2012 한국진공학회 학술발표회초록집 Vol.2012 No.2

Unexpected Nondissociative Binding of N₂O on Oxygen Vacancies on a Rutile TiO₂(110)-1×1

Kim, Boseong,Li, Zhenjun,Kay, Bruce D.,Dohná,lek, Zdenek,Kim, Yu Kwon American Chemical Society 2012 The Journal of Physical Chemistry Part C Vol. No.

<P>The interaction of N<SUB>2</SUB>O with oxygen vacancies (V<SUB>O</SUB>’s) on a partially reduced rutile TiO<SUB>2</SUB>(110)-1×1 surface was investigated using temperature-programmed desorption (TPD). Contrary to a common belief that V<SUB>O</SUB> on a rutile TiO<SUB>2</SUB>(110) is a dissociation site for N<SUB>2</SUB>O, our results indicate that N<SUB>2</SUB>O does not dissociate to form N<SUB>2</SUB>(g) and O(a). In TPD, N<SUB>2</SUB>O desorption shows two peaks with maxima at 135 and 175 K that are assigned to N<SUB>2</SUB>O desorption from Ti<SUP>4+</SUP> and V<SUB>O</SUB> sites, respectively, with absolute coverages determined to be 5.4 × 10<SUP>14</SUP> N<SUB>2</SUB>O/cm<SUP>2</SUP> and 2.3 × 10<SUP>13</SUP> N<SUB>2</SUB>O/cm<SUP>2</SUP>, respectively, on the TiO<SUB>2</SUB>(110)-1×1 surface used (V<SUB>O</SUB> concentration of 5%, 2.6 × 10<SUP>13</SUP>/cm<SUP>2</SUP>). When V<SUB>O</SUB>’s are passivated by dissociative adsorption of H<SUB>2</SUB>O, the N<SUB>2</SUB>O desorption peak at 175 K disappears, evidencing that the peak is related to V<SUB>O</SUB>-bonded N<SUB>2</SUB>O. The absence of N<SUB>2</SUB>O dissociation on V<SUB>O</SUB>’s is supported by a number of observations. First, the integrated amount of N<SUB>2</SUB>O desorbed from the substrate during TPD vs the amount of N<SUB>2</SUB>O dosed at 70 K shows a straight line with no offset, indicating no loss of N<SUB>2</SUB>O due to the N<SUB>2</SUB> formation. Second, N<SUB>2</SUB>O scattering experiments at 300–350 K indicate no change in the V<SUB>O</SUB> concentration as determined from the H<SUB>2</SUB>O TPD spectra. Third, N<SUB>2</SUB>O uptake experiments at 70–90 K show that the N<SUB>2</SUB> desorption feature is observed from TiO<SUB>2</SUB>(110) surfaces without V<SUB>O</SUB>’s, suggesting a possible contribution from background N<SUB>2</SUB> adsorption. On the basis of the above observations, we conclude that N<SUB>2</SUB>O does not dissociate on V<SUB>O</SUB> sites on TiO<SUB>2</SUB>(110), in contrast with the currently accepted view.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2012/jpccck.2012.116.issue-1/jp210636j/production/images/medium/jp-2011-10636j_0007.gif'></P>

Low-Temperature Desorption of N₂O from NO on Rutile TiO₂(110)-1 × 1

Kim, Boseong,Li, Zhenjun,Kay, Bruce D.,Dohná,lek, Zdenek,Kim, Yu Kwon American Chemical Society 2014 The Journal of Physical Chemistry Part C Vol.118 No.18

<P>We find that NO dosed on rutile TiO<SUB>2</SUB>(110)-1 × 1 at substrate temperatures as low as 50 K readily reacts to produce N<SUB>2</SUB>O, which desorbs promptly from the surface leaving an oxygen adatom behind. The desorption rate of N<SUB>2</SUB>O reaches a maximum value after 1–2 s at an NO flux of 1.2 × 10<SUP>14</SUP> NO/cm<SUP>2</SUP>·sec and then decreases rapidly as the initially clean, reduced TiO<SUB>2</SUB>(110) surface with ∼5% oxygen vacancies (V<SUB>O</SUB>’s) becomes covered with oxygen adatoms and unreacted NO. The maximum desorption rate is also found to increase as the substrate temperature is raised up to about 100 K. Interestingly, the N<SUB>2</SUB>O desorption during the low-temperature (LT) NO dose is strongly suppressed when molecular oxygen is predosed, whereas it persists on the surface with V<SUB>O</SUB>’s passivated by surface hydroxyls. Our results show that the surface charge, not the V<SUB>O</SUB> sites, plays a dominant role in the LT N<SUB>2</SUB>O desorption induced by a facile NO reduction at such low temperatures.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2014/jpccck.2014.118.issue-18/jp501179y/production/images/medium/jp-2014-01179y_0006.gif'></P>

The effect of oxygen vacancies on the binding interactions of NH₃ with rutile TiO₂(110)-1 × 1

Kim, Boseong,Li, Zhenjun,Kay, Bruce D.,Dohná,lek, Zdenek,Kim, Yu Kwon The Royal Society of Chemistry 2012 Physical chemistry chemical physics Vol.14 No.43

<P>A series of NH<SUB>3</SUB> temperature-programmed desorption (TPD) spectra were taken after dosing NH<SUB>3</SUB> at 70 K on rutile TiO<SUB>2</SUB>(110)-1 × 1 surfaces with oxygen vacancy (V<SUB>O</SUB>) concentrations of ∼0% (p-TiO<SUB>2</SUB>) and 5% (r-TiO<SUB>2</SUB>), respectively, to study the effect of V<SUB>O</SUB>s on the desorption energy of NH<SUB>3</SUB> as a function of coverage, <I>&thetas;</I>. Our results show that in the zero coverage limit, the desorption energy of NH<SUB>3</SUB> on r-TiO<SUB>2</SUB> is 115 kJ mol<SUP>−1</SUP>, which is 10 kJ mol<SUP>−1</SUP> less than that on p-TiO<SUB>2</SUB>. The desorption energy from the Ti<SUP>4+</SUP> sites decreases with increasing <I>&thetas;</I> due to repulsive NH<SUB>3</SUB>–NH<SUB>3</SUB> interactions and approaches ∼55 kJ mol<SUP>−1</SUP> upon the saturation of Ti<SUP>4+</SUP> sites (<I>&thetas;</I> = 1 monolayer, ML) on both p- and r-TiO<SUB>2</SUB>. The absolute monolayer saturation coverage is determined to be about 10% smaller on r-TiO<SUB>2</SUB> than that on p-TiO<SUB>2</SUB>. Additionally, the trailing edges of the NH<SUB>3</SUB> TPD spectra on the hydroxylated TiO<SUB>2</SUB>(110) (h-TiO<SUB>2</SUB>) appear to be the same as that on r-TiO<SUB>2</SUB> while those on oxidized TiO<SUB>2</SUB>(110) (o-TiO<SUB>2</SUB>) shift to higher temperatures. We present a detailed analysis of the results and reconcile the observed differences based on the repulsive adsorbate–adsorbate dipole interactions between neighboring NH<SUB>3</SUB> molecules and the surface charge associated with the presence of V<SUB>O</SUB>s.</P> <P>Graphic Abstract</P><P>A series of NH<SUB>3</SUB> temperature-programmed desorption (TPD) spectra were taken after dosing NH<SUB>3</SUB> at 70 K on rutile TiO<SUB>2</SUB>(110)-1 × 1 surfaces with oxygen vacancy (V<SUB>O</SUB>) concentrations of ∼0% (p-TiO<SUB>2</SUB>) and 5% (r-TiO<SUB>2</SUB>), respectively, to study the effect of V<SUB>O</SUB>'s on the desorption energy of NH<SUB>3</SUB> as a function of coverage, <I>&thetas;</I>. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2cp42754k'> </P>

Study on Strength Characteristics and Microstructure of Completely Decomposed Migmatitic Granite

Song Yan,Hua Tang,Zhenjun Wu,Sitao Li,Peifeng Li 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.1

Migmatitic granite is the product of mixed lithification and granitization, and completely decomposed migmatitic granite (CDMG) was formed after weathering. The engineering properties of CDMG are complex, and its engineering properties are complex after weathering, and water content has a great influence on the properties of (CDMG). Due to its loose structure and strong heterogeneity, it is difficult to determine its strength characteristics by conventional test methods. In the paper triaxial and micro-CT test were conducted to study the relationship between shear strength and microstructure of CDMG under different water content. The results show that with the increase of water content from 6% to 14%, the internal friction angle decreases from 31.8° to 27.3°, and the cohesion first increases from 76.5 kPa to 94.3 kPa and then decreases to 77.7 kPa. This is because the sliding friction coefficient between coarse particles decreases with the increase of water film thickness on the surface of particles, and the occlusal effect between coarse particles weakens and the displacement adjustment is easier during the triaxial loading process. Under different water content, the shear strength of CDMG is not sensitive to particle structure parameters including particle size, particle morphology and particle arrangement. The particle analysis results of post-test sample show that the crushing proportion of coarse particles increases with the increase of the internal friction angle. The clay minerals produce differential expansion potential with the increase of water content, and the porosity and pore connectivity first decrease and then increase. Cohesion has a linear relationship with porosity and pore connectivity. With the increase of porosity from 23.95% to 26.95%, cohesion decreases linearly by 17%. These results indicate that the internal friction angle of CDMG can be inferred by water content, and the cohesion can be inferred by porosity and pore connectivity obtained by microstructure analysis.

Simultaneous distillation-extraction for manufacturing ultra-high-purity electronic-grade octamethylcyclotetrasiloxane (D4)

Wenhui Guo,Shuhu Guo,Xu Zhao,Zhenjun Yuan,Yu Zhao,Xin Chang,Hong Li,Xiong Zhao,Ye Wan,Dazhou Yan,Zhongyuan Ren,Xiaolei Fan,Xin Gao 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.109 No.-

Ultra-high-purity (UHP) electronic-grade octamethylcyclotetrasiloxane (D4) is the key precursor of lowdielectricconstant (low-k) SiCOH films to manufacture integrated circuits (IC), meeting the stringentrequirements of the rapidly developing semiconductor industry. Commonly, metallic impurities in D4were removed by multiple unit operations of adsorption, filtration, and distillation, which could reducethe concentration of a single metallic impurity below 1 ppb. However, D4 with higher purity is requiredby semiconductor production due to an increase in transistor density. Herein, a novel method based onthe integrated simultaneous distillation–extraction (SDE) was developed for manufacturing UHPelectronic-grade D4. The lab and pilot scale experiments showed that the purity of water and D4 has apositive correlation. Based on the experimental data, a double-column process, consisting of azeotropic/extractive distillation column and precision distillation column with UNIQUAC method, was establishedto access the feasibility of scaling up the SDE process. According to the simulation results, D4with the purity > 99.999 wt.% and total metallic impurities (TMI) content below 1 ppb could be obtainedusing ultra-pure water.

Heterologous Expression and Characterization of a Laccase from Laccaria bicolor in Pichia pastoris and Arabidopsis thaliana

( Bo Wang ),( Ying Yan ),( Jing Xu ),( Xiaoyan Fu ),( Hongjuan Han ),( Jianjie Gao ),( Zhenjun Li ),( Lijuan Wang ),( Yongsheng Tian ),( Rihe Peng ),( Quanhong Yao ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12

Laccases can oxidize a variety of phenolic and non-phenolic substrates including synthetic dyes. In this research, a laccase gene Lcc9 from Laccaria bicolor was chemically synthesized and optimized to heterogeneous expression in Pichia pastoris and Arabidopsis thaliana. The properties of recombinant laccase expressed by P. pastoris were investigated. The laccase activity was optimal at 3.6 pH and 40°C. It exhibited K_m and V_max values of 0.565 mmol l^-1 and 1.51 μmol l^-1 min^-1 for ABTS respectively. As compared with untransformed control plants, the laccase activity in crude extracts of transgenic lines exhibited a 5.4 to 12.4-fold increase. Both laccases expressed in transgenic P. pastoris or A. thaliana could decolorize crystal violet. These results indicated that L. bicolor laccase gene may be transgenically exploited in fungi or plants for dye decolorization.

Isolation and characterization of tick-borne Roseomonas haemaphysalidis sp. nov. and rodent-borne Roseomonas marmotae sp. nov.

Zhu Wentao,Zhou Juan,Lu Shan,Yang Jing,Lai Xin-He,Jin Dong,Pu Ji,Huang Yuyuan,Liu Liyun,Li Zhenjun,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.2

Four novel Gram-negative, mesophilic, aerobic, motile, and cocci-shaped strains were isolated from tick samples (strains 546T and 573) and respiratory tracts of marmots (strains 1318T and 1311). The 16S rRNA gene sequencing revealed that strains 546T and 573 were 97.8% identical to Roseomonas wenyumeiae Z23T, whereas strains 1311 and 1318T were 98.3% identical to Roseomonas ludipueritiae DSM 14915T. In addition, a 98.0% identity was observed between strains 546T and 1318T. Phylogenetic and phylogenomic analyses revealed that strains 546T and 573 clustered with R. wenyumeiae Z23T, whereas strains 1311 and 1318T grouped with R. ludipueritiae DSM 14915T. The average nucleotide identity between our isolates and members of the genus Roseomonas was below 95%. The genomic G+C content of strains 546T and 1318T was 70.9% and 69.3%, respectively. Diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE) were the major polar lipids, with Q-10 as the predominant respiratory quinone. According to all genotypic, phenotypic, phylogenetic, and phylogenomic analyses, the four strains represent two novel species of the genus Roseomonas, for which the names Roseomonas haemaphysalidis sp. nov. and Roseomonas marmotae sp. nov. are proposed, with 546T (= GDMCC 1.1780T = JCM 34187T) and 1318T (= GDMCC 1.1781T = JCM 34188T) as type strains, respectively.

Gut Microbiota Community and Its Assembly Associated with Age and Diet in Chinese Centenarians

( Fang Wang ),( Ting Yu ),( Guo Hong Huang ),( Da Cai ),( Xiaolin Liang ),( Hai Yan Su ),( Zhenjun Zhu ),( Danlei Li ),( Yang Yang ),( Pei Hong Shen ),( Rui Feng Mao ),( Lian Yu ),( Mou Ming Zhao ),( 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.8

Increasing evidence suggests that gut microbiota underpin the development of health and longevity. However, our understanding of what influences the composition of this community of the longevous has not been adequately described. Therefore, illumina sequencing analysis was performed on the gut microbiota of centenarians (aged 100-108 years; RC) and younger elderlies (aged 85-99 years; RE) living in Bama County, Guangxi, China and the elderlies (aged 80-92 years; CE) living in Nanning City, Guangxi, China. In addition, their diet was monitored using a semiquantitative dietary questionary (FFQ 23). The results revealed the abundance of Roseburia and Escherichia was significantly greater, whereas that of Lactobacillus, Faecalibacterium, Parabacteroides, Butyricimonas, Coprococcus, Megamonas, Mitsuokella, Sutterella, and Akkermansia was significantly less in centenarians at the genus level. Both clustering analysis and UniFraq distance analysis showed structural segregation with age and diet among the three populations. Using partial least square discriminate analysis and redundancy analysis, we identified 33 and 34 operational taxonomic units (OTUs) as key OTUs that were significantly associated with age and diet, respectively. Age-related OTUs were characterized as Ruminococcaceae, Clostridiaceae, and Lachnospiraceae, and the former two were increased in the centenarians; diet-related OTUs were classified as Bacteroidales, Lachnospiraceae, and Ruminococcaceae. The former two were deceased, whereas the later one was increased, in the high-fiber diet. The age and high-fiber diet were concomitant with changes in the gut microbiota of centenarians, suggesting that age and high-fiber diet can establish a new structurally balanced architecture of gut microbiota that may benefit the health of centenarians.