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- 저자 : ( Yongsheng Tian ) (검색결과 4 건)
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Murine Model Study of a New Receptor-Targeted Tracer for Sentinel Lymph Node in Breast Cancer
Chonglin Tian,Xiao Sun,Bin-Bin Cong,Pengfei Qiu,Yongsheng Wang 한국유방암학회 2019 Journal of breast cancer Vol.22 No.2
Purpose: Sentinel lymph node biopsy (SLNB), a critical staging and treatment step, has replaced axillary lymph node (LN) dissection as the standard staging procedure for early stage breast cancer patients with clinically negative axillary LNs. Hence, using a murine sentinel lymph node (SLN) model, we investigated the localization effect of the new receptor-targeted tracer, indocyanine green (ICG)-rituximab, on breast cancer SLNB. Methods: After establishing the murine SLN model, different doses of ICG-rituximab were subcutaneously injected into the hind insteps of BALB/c mice to determine the optimal dose and imaging time using continuous (> 3 hours) MDM-I fluorescence vasculature imaging. To explore the capacity of ICG-rituximab for sustained SLN localization with the optimal dose, MDM-I imaging was monitored at 6, 12, and 24 hours. Results: The popliteal LN was defined as the SLN for hindlimb lymphatic drainage, the iliac LN as the secondary, and the para-aortic or renal LN as the tertiary LNs. The SLN initial imaging and optimal imaging times were shortened with increased ICG-rituximab doses, and the imaging rates of the secondary and tertiary LNs increased accordingly. The optimal ICG dose was 0.12 μg, and its optimal imaging time was 34 minutes. After 24 hours, the SLN imaging rate remained 100%, while those of the secondary and the tertiary LNs increased from 0% (6 hours) and 0% (6 hours) to 10% (12 hours) and 10% (12 hours) to 20% (24 hours) and 10% (24 hours), respectively. Conclusion: ICG-rituximab localized to the SLN without imaging from the secondary or tertiary LNs within 6 hours. The optimal ICG dose was 0.12 μg, and the optimal interval for SLN detection was 34 minutes to 6 hours post-injection. This novel receptor-targeted tracer is of great value to clinical research and application.
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Shuo Zhang,Jiantao Yao,Ruochao Wang,Yu Tian,Jiaxin Wang,Yongsheng Zhao 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.1
This paper mainly studies the optimization of the patrol route of a fire inspection robot, based on analytic hierarchy process (AHP) and fire source detection. First, the A* algorithm is improved based on two aspects of the heuristic function and of the obstacle boundary setting, while the suboptimal path is obtained in MATLAB. Next, the path planned according to the improved A* algorithm is smoothed and optimized, by means of gradient descent method, Bezier curve and B-spline curve, while the index parameters are optimized by means of MATLAB simulation. In view of the simulation results, the trajectory optimization performance index evaluation system, established by five decision criteria, including running time, path length, ride comfort, no-collision effect and quadratic optimization space, is put forward. The three kinds of optimization methods are analyzed qualitatively and quantitatively, and the results show that, in the total hierarchical ranking, the B-spline curve trajectory optimization scheme has the largest weight and is more important than the other two schemes. Finally, the superiority of B-spline curve is verified experimentally.
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Ziyu Song,Meng Jin,Shenglong Wang,Yanzuo Wu,Qi Huang,Wangda Xu,Yongsheng Fan,Fengyuan Tian The Korean Society of Ginseng 2024 Journal of Ginseng Research Vol.48 No.2
Background: Deposition of immune complexes drives podocyte injury acting in the initial phase of lupus nephritis (LN), a process mediated by B cell involvement. Accordingly, targeting B cell subsets represents a potential therapeutic approach for LN. Ginsenoside compound K (CK), a bioavailable component of ginseng, possesses nephritis benefits in lupus-prone mice; however, the underlying mechanisms involving B cell subpopulations remain elusive. Methods: Female MRL/lpr mice were administered CK (40 mg/kg) intragastrically for 10 weeks, followed by measurements of anti-dsDNA antibodies, inflammatory chemokines, and metabolite profiles on renal samples. Podocyte function and ultrastructure were detected. Publicly available single-cell RNA sequencing data and flow cytometry analysis were employed to investigate B cell subpopulations. Metabolomics analysis was adopted. SIRT1 and AMPK expression were analyzed by immunoblotting and immunofluorescence assays. Results: CK reduced proteinuria and protected podocyte ultrastructure in MRL/lpr mice by suppressing circulating anti-dsDNA antibodies and mitigating systemic inflammation. It activated B cell-specific SIRT1 and AMPK with Rhamnose accumulation, hindering the conversion of renal B cells into plasma cells. This cascade facilitated the resolution of local renal inflammation. CK facilitated the clearance of deposited immune complexes, thus reinstating podocyte morphology and mobility by normalizing the expression of nephrin and SYNPO. Conclusions: Our study reveals the synergistic interplay between SIRT1 and AMPK, orchestrating the restoration of renal B cell subsets. This process effectively mitigates immune complex deposition and preserves podocyte function. Accordingly, CK emerges as a promising therapeutic agent, potentially alleviating the hyperactivity of renal B cell subsets during LN.
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( Bo Wang ),( Ying Yan ),( Jing Xu ),( Xiaoyan Fu ),( Hongjuan Han ),( Jianjie Gao ),( Zhenjun Li ),( Lijuan Wang ),( Yongsheng Tian ),( Rihe Peng ),( Quanhong Yao ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12
Laccases can oxidize a variety of phenolic and non-phenolic substrates including synthetic dyes. In this research, a laccase gene Lcc9 from Laccaria bicolor was chemically synthesized and optimized to heterogeneous expression in Pichia pastoris and Arabidopsis thaliana. The properties of recombinant laccase expressed by P. pastoris were investigated. The laccase activity was optimal at 3.6 pH and 40°C. It exhibited K<sub>m</sub> and V<sub>max</sub> values of 0.565 mmol l<sup>-1</sup> and 1.51 μmol l<sup>-1</sup> min<sup>-1</sup> for ABTS respectively. As compared with untransformed control plants, the laccase activity in crude extracts of transgenic lines exhibited a 5.4 to 12.4-fold increase. Both laccases expressed in transgenic P. pastoris or A. thaliana could decolorize crystal violet. These results indicated that L. bicolor laccase gene may be transgenically exploited in fungi or plants for dye decolorization.
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