Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury

Yu Zhenjun,Li Yuhan,Shao Shuai,Guo Beichen,Zhang Mengxia,Zheng Lina,Zhang Kun,Zhou Feng,Zhang Li,Chen Chiyi,Jiang Wentao,Hong Wei,Han Tao 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Some long noncoding RNAs (lncRNAs), which harbor microRNAs in their gene sequence and are also known as microRNA host gene derived lncRNAs (lnc-MIRHGs), play a dominant role alongside miRNAs, or both perform biological functions synergistically or independently. However, only a small number of lnc-MIRHGs have been identified. Here, multiple liver injury datasets were analyzed to screen and identify the target lncRNA Mir122hg. Mir122hg was mainly enriched in liver tissues with human-mouse homology. In both CCl4-induced acute liver injury and Dgal/LPS-induced fulminant liver failure in mice, Mir122hg was sharply downregulated at the early stage, while a subsequent significant increase was only found in the CCl4 group with liver recovery. Overexpression and silencing assays confirmed that Mir122hg played a protective role in acute injury by promoting hepatocyte proliferation in vivo and in vitro. Consistent with the results of gene enrichment analysis, Mir122hg binding to C/EBPα affected its transcriptional repression, promoted gene transcription of downstream chemokines, Cxcl2, Cxcl3, and Cxcl5, and exerted pro-proliferative effects on hepatocytes through activation of the AKT/GSK-3β/p27 signaling pathway by CXC/CXCR2 complexes. This study identifies a novel lncRNA with protective effects in acute liver injury and demonstrates that the binding of Mir122hg-C/EBPα promotes hepatocyte proliferation via upregulation of CXC chemokine and activation of AKT signaling.

Low-Temperature Desorption of N₂O from NO on Rutile TiO₂(110)-1 × 1

Kim, Boseong,Li, Zhenjun,Kay, Bruce D.,Dohná,lek, Zdenek,Kim, Yu Kwon American Chemical Society 2014 The Journal of Physical Chemistry Part C Vol.118 No.18

<P>We find that NO dosed on rutile TiO<SUB>2</SUB>(110)-1 × 1 at substrate temperatures as low as 50 K readily reacts to produce N<SUB>2</SUB>O, which desorbs promptly from the surface leaving an oxygen adatom behind. The desorption rate of N<SUB>2</SUB>O reaches a maximum value after 1–2 s at an NO flux of 1.2 × 10<SUP>14</SUP> NO/cm<SUP>2</SUP>·sec and then decreases rapidly as the initially clean, reduced TiO<SUB>2</SUB>(110) surface with ∼5% oxygen vacancies (V<SUB>O</SUB>’s) becomes covered with oxygen adatoms and unreacted NO. The maximum desorption rate is also found to increase as the substrate temperature is raised up to about 100 K. Interestingly, the N<SUB>2</SUB>O desorption during the low-temperature (LT) NO dose is strongly suppressed when molecular oxygen is predosed, whereas it persists on the surface with V<SUB>O</SUB>’s passivated by surface hydroxyls. Our results show that the surface charge, not the V<SUB>O</SUB> sites, plays a dominant role in the LT N<SUB>2</SUB>O desorption induced by a facile NO reduction at such low temperatures.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2014/jpccck.2014.118.issue-18/jp501179y/production/images/medium/jp-2014-01179y_0006.gif'></P>

The effect of oxygen vacancies on the binding interactions of NH₃ with rutile TiO₂(110)-1 × 1

Kim, Boseong,Li, Zhenjun,Kay, Bruce D.,Dohná,lek, Zdenek,Kim, Yu Kwon The Royal Society of Chemistry 2012 Physical chemistry chemical physics Vol.14 No.43

<P>A series of NH<SUB>3</SUB> temperature-programmed desorption (TPD) spectra were taken after dosing NH<SUB>3</SUB> at 70 K on rutile TiO<SUB>2</SUB>(110)-1 × 1 surfaces with oxygen vacancy (V<SUB>O</SUB>) concentrations of ∼0% (p-TiO<SUB>2</SUB>) and 5% (r-TiO<SUB>2</SUB>), respectively, to study the effect of V<SUB>O</SUB>s on the desorption energy of NH<SUB>3</SUB> as a function of coverage, <I>&thetas;</I>. Our results show that in the zero coverage limit, the desorption energy of NH<SUB>3</SUB> on r-TiO<SUB>2</SUB> is 115 kJ mol<SUP>−1</SUP>, which is 10 kJ mol<SUP>−1</SUP> less than that on p-TiO<SUB>2</SUB>. The desorption energy from the Ti<SUP>4+</SUP> sites decreases with increasing <I>&thetas;</I> due to repulsive NH<SUB>3</SUB>–NH<SUB>3</SUB> interactions and approaches ∼55 kJ mol<SUP>−1</SUP> upon the saturation of Ti<SUP>4+</SUP> sites (<I>&thetas;</I> = 1 monolayer, ML) on both p- and r-TiO<SUB>2</SUB>. The absolute monolayer saturation coverage is determined to be about 10% smaller on r-TiO<SUB>2</SUB> than that on p-TiO<SUB>2</SUB>. Additionally, the trailing edges of the NH<SUB>3</SUB> TPD spectra on the hydroxylated TiO<SUB>2</SUB>(110) (h-TiO<SUB>2</SUB>) appear to be the same as that on r-TiO<SUB>2</SUB> while those on oxidized TiO<SUB>2</SUB>(110) (o-TiO<SUB>2</SUB>) shift to higher temperatures. We present a detailed analysis of the results and reconcile the observed differences based on the repulsive adsorbate–adsorbate dipole interactions between neighboring NH<SUB>3</SUB> molecules and the surface charge associated with the presence of V<SUB>O</SUB>s.</P> <P>Graphic Abstract</P><P>A series of NH<SUB>3</SUB> temperature-programmed desorption (TPD) spectra were taken after dosing NH<SUB>3</SUB> at 70 K on rutile TiO<SUB>2</SUB>(110)-1 × 1 surfaces with oxygen vacancy (V<SUB>O</SUB>) concentrations of ∼0% (p-TiO<SUB>2</SUB>) and 5% (r-TiO<SUB>2</SUB>), respectively, to study the effect of V<SUB>O</SUB>'s on the desorption energy of NH<SUB>3</SUB> as a function of coverage, <I>&thetas;</I>. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2cp42754k'> </P>

Unexpected Nondissociative Binding of N₂O on Oxygen Vacancies on a Rutile TiO₂(110)-1×1

Kim, Boseong,Li, Zhenjun,Kay, Bruce D.,Dohná,lek, Zdenek,Kim, Yu Kwon American Chemical Society 2012 The Journal of Physical Chemistry Part C Vol. No.

<P>The interaction of N<SUB>2</SUB>O with oxygen vacancies (V<SUB>O</SUB>’s) on a partially reduced rutile TiO<SUB>2</SUB>(110)-1×1 surface was investigated using temperature-programmed desorption (TPD). Contrary to a common belief that V<SUB>O</SUB> on a rutile TiO<SUB>2</SUB>(110) is a dissociation site for N<SUB>2</SUB>O, our results indicate that N<SUB>2</SUB>O does not dissociate to form N<SUB>2</SUB>(g) and O(a). In TPD, N<SUB>2</SUB>O desorption shows two peaks with maxima at 135 and 175 K that are assigned to N<SUB>2</SUB>O desorption from Ti<SUP>4+</SUP> and V<SUB>O</SUB> sites, respectively, with absolute coverages determined to be 5.4 × 10<SUP>14</SUP> N<SUB>2</SUB>O/cm<SUP>2</SUP> and 2.3 × 10<SUP>13</SUP> N<SUB>2</SUB>O/cm<SUP>2</SUP>, respectively, on the TiO<SUB>2</SUB>(110)-1×1 surface used (V<SUB>O</SUB> concentration of 5%, 2.6 × 10<SUP>13</SUP>/cm<SUP>2</SUP>). When V<SUB>O</SUB>’s are passivated by dissociative adsorption of H<SUB>2</SUB>O, the N<SUB>2</SUB>O desorption peak at 175 K disappears, evidencing that the peak is related to V<SUB>O</SUB>-bonded N<SUB>2</SUB>O. The absence of N<SUB>2</SUB>O dissociation on V<SUB>O</SUB>’s is supported by a number of observations. First, the integrated amount of N<SUB>2</SUB>O desorbed from the substrate during TPD vs the amount of N<SUB>2</SUB>O dosed at 70 K shows a straight line with no offset, indicating no loss of N<SUB>2</SUB>O due to the N<SUB>2</SUB> formation. Second, N<SUB>2</SUB>O scattering experiments at 300–350 K indicate no change in the V<SUB>O</SUB> concentration as determined from the H<SUB>2</SUB>O TPD spectra. Third, N<SUB>2</SUB>O uptake experiments at 70–90 K show that the N<SUB>2</SUB> desorption feature is observed from TiO<SUB>2</SUB>(110) surfaces without V<SUB>O</SUB>’s, suggesting a possible contribution from background N<SUB>2</SUB> adsorption. On the basis of the above observations, we conclude that N<SUB>2</SUB>O does not dissociate on V<SUB>O</SUB> sites on TiO<SUB>2</SUB>(110), in contrast with the currently accepted view.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2012/jpccck.2012.116.issue-1/jp210636j/production/images/medium/jp-2011-10636j_0007.gif'></P>

Gut Microbiota Community and Its Assembly Associated with Age and Diet in Chinese Centenarians

( Fang Wang ),( Ting Yu ),( Guo Hong Huang ),( Da Cai ),( Xiaolin Liang ),( Hai Yan Su ),( Zhenjun Zhu ),( Danlei Li ),( Yang Yang ),( Pei Hong Shen ),( Rui Feng Mao ),( Lian Yu ),( Mou Ming Zhao ),( 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.8

Increasing evidence suggests that gut microbiota underpin the development of health and longevity. However, our understanding of what influences the composition of this community of the longevous has not been adequately described. Therefore, illumina sequencing analysis was performed on the gut microbiota of centenarians (aged 100-108 years; RC) and younger elderlies (aged 85-99 years; RE) living in Bama County, Guangxi, China and the elderlies (aged 80-92 years; CE) living in Nanning City, Guangxi, China. In addition, their diet was monitored using a semiquantitative dietary questionary (FFQ 23). The results revealed the abundance of Roseburia and Escherichia was significantly greater, whereas that of Lactobacillus, Faecalibacterium, Parabacteroides, Butyricimonas, Coprococcus, Megamonas, Mitsuokella, Sutterella, and Akkermansia was significantly less in centenarians at the genus level. Both clustering analysis and UniFraq distance analysis showed structural segregation with age and diet among the three populations. Using partial least square discriminate analysis and redundancy analysis, we identified 33 and 34 operational taxonomic units (OTUs) as key OTUs that were significantly associated with age and diet, respectively. Age-related OTUs were characterized as Ruminococcaceae, Clostridiaceae, and Lachnospiraceae, and the former two were increased in the centenarians; diet-related OTUs were classified as Bacteroidales, Lachnospiraceae, and Ruminococcaceae. The former two were deceased, whereas the later one was increased, in the high-fiber diet. The age and high-fiber diet were concomitant with changes in the gut microbiota of centenarians, suggesting that age and high-fiber diet can establish a new structurally balanced architecture of gut microbiota that may benefit the health of centenarians.

Simultaneous distillation-extraction for manufacturing ultra-high-purity electronic-grade octamethylcyclotetrasiloxane (D4)

Wenhui Guo,Shuhu Guo,Xu Zhao,Zhenjun Yuan,Yu Zhao,Xin Chang,Hong Li,Xiong Zhao,Ye Wan,Dazhou Yan,Zhongyuan Ren,Xiaolei Fan,Xin Gao 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.109 No.-

Ultra-high-purity (UHP) electronic-grade octamethylcyclotetrasiloxane (D4) is the key precursor of lowdielectricconstant (low-k) SiCOH films to manufacture integrated circuits (IC), meeting the stringentrequirements of the rapidly developing semiconductor industry. Commonly, metallic impurities in D4were removed by multiple unit operations of adsorption, filtration, and distillation, which could reducethe concentration of a single metallic impurity below 1 ppb. However, D4 with higher purity is requiredby semiconductor production due to an increase in transistor density. Herein, a novel method based onthe integrated simultaneous distillation–extraction (SDE) was developed for manufacturing UHPelectronic-grade D4. The lab and pilot scale experiments showed that the purity of water and D4 has apositive correlation. Based on the experimental data, a double-column process, consisting of azeotropic/extractive distillation column and precision distillation column with UNIQUAC method, was establishedto access the feasibility of scaling up the SDE process. According to the simulation results, D4with the purity > 99.999 wt.% and total metallic impurities (TMI) content below 1 ppb could be obtainedusing ultra-pure water.