Self-Assembling Peptides and Their Application in the Treatment of Diseases

Lee,,Trinh,,Yoo,,Shin,,Lee,,Kim,,Hwang,,Lim,,Ryou, MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.23

<P>Self-assembling peptides are biomedical materials with unique structures that are formed in response to various environmental conditions. Governed by their physicochemical characteristics, the peptides can form a variety of structures with greater reactivity than conventional non-biological materials. The structural divergence of self-assembling peptides allows for various functional possibilities; when assembled, they can be used as scaffolds for cell and tissue regeneration, and vehicles for drug delivery, conferring controlled release, stability, and targeting, and avoiding side effects of drugs. These peptides can also be used as drugs themselves. In this review, we describe the basic structure and characteristics of self-assembling peptides and the various factors that affect the formation of peptide-based structures. We also summarize the applications of self-assembling peptides in the treatment of various diseases, including cancer. Furthermore, the in-cell self-assembly of peptides, termed reverse self-assembly, is discussed as a novel paradigm for self-assembling peptide-based nanovehicles and nanomedicines.</P>

조혈모세포이식 환자에서 발생한 Cytomegalovirus 질환의 특징 : 일개 대학변원에서 최근 10년간의 경험

최수미,이동건,박선희,김시현,김유진,민창기,김희제,이석,최정현,유진홍,김동욱,이종욱,민우성,신완식,김춘추 대한감염학회 2009 감염과 화학요법 Vol.41 No.1

Background : Studies on cytomegalovirus (CMV) diseases in Korean hematopoietic stem cell transplant (HSCT) recipients are lacking and do not reflect the recent trends of advances and changes. Therefore, we tried to analyze the clinical features of CMV diseases in HSCT recipients over the past 10 years at a tertiary university hospital in Korea. Methods : Retrospective review of medical records was done for all adult HSCT patients who received transplant at the Catholic HSCT Center from January 1998 to January 2008. Results : Forty-four cases (2.2%) of CMV diseases were identified. CMV pneumonia was diagnosed in 17 patients, retinitis in 16 patients, enterocolitis in 7 patients, esophagitis 1 patient, gastritis in 1 patient, duodenitis in 1 patient, and hepatitis in 1 patient. The median onset of symptom was 90 days after transplantation. Late CMV diseases accounted for 47.7%. CMV related death varied from 0 to 58.8% according to the involved organ. CMV retinitis was diagnosed relatively later in the course of transplantation mostly in patients who had chronic graft versus host disease (GVHD). On the contrary, CMV enterocolitis mainly occurred in patients who suffered from acute GVHD. The overall concurrent CMV reactivation was documented to be 63.6%: the concurrent CMV reactivation was observed only in 37.5% of patients with retinitis. Conclusions : We observed some differences in the pattern of CMV disease manifestation according to the involved organ and reconfirmed the fact that CMV pneumonia is the most common and fatal disease in HSCT recipients. Additionally, CMV retinitis was not uncommon in HSCT recipients. Since specific marker does not exist in predicting retinitis, regular ocular examination should be done thoroughly, especially in patients with chronic GVHD.

Novel Methylation Biomarker for Non-invasive Diagnostics in Lung Cancer

오태정,( Chang Hun Lee2 ),( Min Ki Lee ),( Yeul Hong Kim ),( Sang Yull Lee ),( Hyo Sung Jeon ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Yong Park ),( Sung Whan An ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

To identify aberrantly hypermethylated DNA in lung cancer cells we established a genome-wide analysis for hypermethylation sites, namely Methyl DNA Isolation and Amplification (MeDIA) coupled-CpG microarray analysis. In the comprehensive methyaltion profiling analysis between human lung cancer, A549 cells and normal NHBE cells, we observed that several clusters of genes show a significant level of aberrancy in CpG island methylation pattern in cancer cells compared to normal cells. We further identified PCDHGA12 gene as a new marker of non-invasive diagnostics for lung cancer based on followings. 1) Transcription of PCDHGA12 gene is reactivated after treatment of A549 cells with demethylating agent. 2) Bisulfide clonal-sequencing reveals that CpG island of PCDHGA12 shows a distinctive differential methylation between two cell lines. 3) Pyrosequencing-based quantitative methylation assay for such region in tumor and non-tumorous tissues from lung cancer patients shows aberrant hypermethylation in 37 (92%) of the 40 tumor tissues. In clinical validation by pyrosequencingin induced-sputum of lung cancer patients (n=87) and healthy controls (n=51), we observed aberrant hypermethylation incident at significantly elevated level in samples derived from lung cancer patients. According to the optimal threshold calculated by ROC curve analysis, sensitivity and specificity of PCDHGA12 was 86.2% and 82.4%, respectively. PCDHGA12 methylation status could be a potential methylation biomarker alone or combined with others for the screen and the detection of relapse of lung cancer.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

악성 림프종 환자에서 발생한 다형홍반

여운철,조광현,이유신,허대석,이승철 ( Un Cheol Yeo,Kwang Hyun Cho,Yoo Shin Lee,Dae Seog Heo,Seung Chul Lee ) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.5

A Case of Erythema Multiforme A ssociated with Malignant Lymphoma Un Cheol Yeo, M.D., Kwang Hyun Cho, M.D., Yoo Shin Lee, M.D., Dae Seog Heo*, M.D., Seoung Chul Lee**, M.D Department of Dermatology and Internal Medicine*, College of Medicine, Seoul National University, Seoul, Korea, Department of Dermatology**, College of Medicine, Inha University, Kyung Gi Do, Korea We report a case of erythema multiforme associated with malignant lymphoma. Fifty-six-year-old male patient visited our clinic complaining generalized skin lesions which were compatible with erythema multiforme clinically and histopathologically. The skin lesions were treatment-resistant, recurrent and persistent for 3 years. In consideration of old age, atypical features of erythema multiforme and no other demonstrable etiology of erythema multiforme, we studied for internal malignancy. Malignant lymphoma at the duodenum was revealed by UGI series, abdominal CT, and endoscopic biopsy. After chemotherapy, the tumor mass and active skin lesions were cleared simultaneously. Erythema multiforme may be regarded as one of the paraneoplastic syndromes.

梅毒의 治療

李惟信 대한의학협회 1976 대한의학협회지 Vol.19 No.10

메치실린 내성 황색 포도상구균(MRSA)에 대한 소독제 및 항생제의 살균력 효능

이화중,나영천,유석근,김동철,김신무 大韓成形外科學會 2002 Archives of Plastic Surgery Vol.29 No.6

주정중독 환자와 정상음주자에 대한 감정상태와 자기 지각, 가정환경, 사회적 지지에 관한 비교 연구

이정식,한광수,이유상,한일우,신용구 용인정신의학연구소 1997 용인정신의학보 Vol.4 No.1

Cis-Dichlorodiammineplatinum이 Mouse 간장의 인산염 분해효소활성에 미치는 영향

유신철,이동준,정호삼,이규식 漢陽大學校環境科學硏究所 1987 環境科學論文集 Vol.8 No.-

cis-Dichlorodiammineplatium(Ⅱ)(cis-DDP)은 최근에 개발된 효과적인 종양화합 요법제인 금속화합물로서 그 작용기작은 아직 불명하나 bifunctional alkylating agent와 유사한 작용 기전을 갖는 것으로 알려져 있다. cis-DDP는 체내에서 가수분해되어 DNA와 반응함을 핵산의 합성을 억제하여 암세포 뿐만 아니라 정상세포에도 영향을 미쳐 출혈성장염, 임파조직과 골수의 저세포증, 신손상, 단백뇨 및 혈뇨등의 부작용을 유발한다. 이에 저자는 cis-DDP가 mouse 간장에서 세포독성으로 작용하여 간실질세포의 변성을 일으키리라 사료되어 간실질세포내의 acid phosphatase, alkaline phosphstase 및 aden-osine triphosphatase의 변동을 조직화학적으로 관찰하였다. 실험동물은 DDY계의 웅성(체중 20g)mouse를 사용하였으며 체중 kg당 6mg의 cis-DDP를 투여한 후, 6시간, 12시간, 24시간 및 48시간 경과후에 희생하여 간조직을 적출하고 10% formalin용액에 고정하고 cryostat로 16㎛두께로 동결절편을 만들었다. 조직화학적인 연구를 위하여 acid phosphatase와 alkaline phosphatase의 활성은 Gomori 방법으로 그리고 adenosine triphosphatase의 활성은 Wachstein-Meisel 방법으로 이들 효소활성을 관찰하여 다음과 같은 결과를 얻었다. 1. Acid phosphatase 활성은 간소엽주변대에서 cis-DDP투여 6시간 경과군의 mouse에서는 약한 양성반응을 12시간 경과군의 mouse에서는 중등도의 양성반응을 48시간 경과군의 mouse에서는 약한 양성반응을 나타내었다. 2. Alkaline phosphatase 활성은 간소엽 주변대에서 cis-DDP투여 12시간, 24시간, 및 48시간 경과군의 mouse에서 중등도의 양성반응을 나타내었다. 3. Adenosine erihosphatase 활성은 간소엽 중심대에서 cis-DDP투여 6시간 경과군의 mouse에서 미약한 양성반응을 나타내었고, 12시간 경과군의 mouse에서는 중등도의 양성반응을 나타내었고, 24시간 및 48시간 경과군의 mouse에서는 각각 중등도 및 강한 양성반응을 나타내었다. 이상의 소견을 종합한바 cis-DDP의 투여로 mouse의 간장 손상을 일으켜 acid phosphstase, alkaline phospatase 및 adenosine triphosphatase 활성은 일시적으로 저하되었다가 회복되는 것으로 사료된다. cis-Dichlorodiammineplatinnm(Ⅱ) (cis-DDP), known as cisplatin, is a metallic compound that has recently been developed as an effective cancer chemotherapeutic agent. The precise mechanism of action of cis-DDP is still unknown. Similarities between the mechanism of action of this drug and bifunctional alkylating agents have been suggested. Upon entering the cell, the platinum compounds will become hydrolyzed, and then they can react with DNA. They act on tumor cells as well as normal cells. Side effects included severe hemorrhagic enterocolitis, severe hypocellularity of the bone marrow and lymphoid tissues, renal lesions, proteinuria and (microscopic henaturia). In this experiment, the author studied the effect of cis-DDP on the mouse liver, histochemically observing the changes in the activities of acid phosphatase, alkaline phosphatase, and adenosine triphosphatase. Male mice (the weight 20g), DDY strain, were used as experimental animals. The experimental animals were sacrificed at 6,12,24 and 48 hours after administration of 6mg of cis-DDP per kilogram of body weight. The specimens obtained from the liver were fixed in 10% formalin solution and sliced at 16㎛ thickness in a frozen cryostat. The activities of acid and alkaline phosphatases were observed by the Gomori's method and the activity of adenosine triphosphatase was observed by the Wachstein Meisel's method for histochemical study. The results are as follows. 1. The acid phosphatase activity was weakly positive at 6 hours and moderately positive at 12 hours and weakly positive at 48 hours after administration in the peripheral zone of the hepatic lobule. 2. The alkaline phosphatase activity was decreased to moderately positive at 12,24 and 48 hours after administration in the peripheral zone of the hepatic lobule. 3. The adenosine triphosphatase activity was trace positive at 6 hours after administration in the central zone of the hepatic lobule and moderately positive at 12 hours after admnistration in the central and peripheral zone of the hepatic lobule. It is suggested that cis-DDP causes changes in activities of acid phosphatase, alkaline phosphatase and adenosine triphophsatase in the liver, due probably to the cytotoxic effect in the parenchymal cells of the liver in mice.

남자 단거리 선수의 등속성 근력측정법에 따른 슬신전근의 속도곡선 및 최대파워분석

유관호,전용태,이신언 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

In recent years, the knee extensor forces of athletes have usually been evaluated by measuring isokinetic output torque. The purpose of this study was to confirm the usefulness of nonmalizing the torque(force)-velocity curve and calculating the maximal power of knee extensor under isokinetic contraction. Seventy two(46 elite, 26 non-elite) Japanese male sprinters were chosen as the subjects in this study. The peak torque of the dominant side of knee extensor was measured by using the isokinetic dynamometer (Cybex Ⅱ+) in three different angular velocities of 60, 180, 300deg/sec. Moreover, the isometric torque (0deg/sec) was measured in 39 athletes, 120 and 240deg/sec of contraction were performed in 12 out of 39 athletes. The exponent equation (F=F0×eav-kv: Fenn 1935) was applied to normalize the torque-velocity curve without including the coefficient of viscosity(k). The maximal power and its optimal velocity was presumed from this torque-velocity curve. The average of measured torque at 0 deg/sec contraction(F0) was lower than that of 60 deg/sec, therefore F0 was presumed as the same as the maximal power. Those parameters were not significantly different when calculated from 3 velocities (60, 180, 300 deg/sec) and 5 velocities (plus 210, 240 deg/sec) in 12 athletes. For this reason, each parameter was calculated from 3 velocities. The maximal torque (F0/BW) was the same between elite and non-elite group (40Nm/㎏). Nevertheless, the coefficient of torque loss(a), maximal power and its optimal velocity were significantly different (-0.1594 : -0.1911, 9.6 : 7.8watt/㎏, 373 : 309 deg/sec, respectively. P<.01 Student-t). It was said that to normalize the torque-velocity curve or to presume the maximal knee extension power and its optimal velocity were useful to assess the muscle function or the performance of athletes under isokinetic contraction.