Effect of Indirubin on Morphology Changes and mRNA Expression of Cytokines in LPS-induced RAW264.7

Ye Jin Hwang(Ye Jin Hwang),Jae Seon Kang(Jae Seon Kang) 대한약학회 2023 약학회지 Vol.67 No.1

This study was conducted to investigate the regulatory effect of Indirubin on LPS-induced inflammation in macrophage. The morphologic changes were shown as widened cytoplasm or biased nucleus from the center of the cell when LPS activated macrophage. The changes in inflammation activity were also estimated with mRNA levels of cytokines under time elapse at 2, 4, 8, 12, and 24 hours after the 10 ng/mL LPS treatment. By measuring those proinflammatory (TNF-α, IL-1β) and anti-inflammatory (IL-10) cytokines at each time set, the relationship between morphologic change and inflammation activity was compared. The highest pro-inflammatory mRNA expression was observed at 2 hours after the LPS treatment, and this was inhibited by those Indirubin standard (Indirubin) and Chungdae extract (CDEX). The mRNA expression level in LPS treated cell was markedly reduced at 8 hours as well as the other groups. However, IL-10, the anti-inflammatory cytokines was not affected by those treatments. The results suggested that the level of inflammation related changes as time elapsed. The current observation that both Indirubin and CDEX are able to inhibit LPS-induced inflammation by reducing the production of inflammatory cytokines. Moreover, the current observation suggested that estimating anti-inflammatory effects should include time-dependent measurements as biomarkers.

Correlation Between Knee Muscle Strength and Maximal Cycling Speed Measured Using 3D Depth Camera in Virtual Reality Environment

Ye Jin Kim(Ye Jin Kim),Hye-seon Jeon(Hye-seon Jeon),Joo-hee Park(Joo-hee Park),Gyeong-Ah Moon(Gyeong-Ah Moon),Yixin Wang(Yixin Wang) 한국전문물리치료학회 2022 한국전문물리치료학회지 Vol.29 No.4

Background: Virtual reality (VR) programs based on motion capture camera are the most convenient and cost-effective approaches for remote rehabilitation. Assessment of physical function is critical for providing optimal VR rehabilitation training; however, direct muscle strength measurement using camera-based kinematic data is impracticable. Therefore, it is necessary to develop a method to indirectly estimate the muscle strength of users from the value obtained using a motion capture camera. Objects: The purpose of this study was to determine whether the pedaling speed converted using the VR engine from the captured foot position data in the VR environment can be used as an indirect way to evaluate knee muscle strength, and to investigate the validity and reliability of a camera-based VR program. Methods: Thirty healthy adults were included in this study. Each subject performed a 15-second maximum pedaling test in the VR and built-in speedometer modes. In the VR speedometer mode, a motion capture camera was used to detect the position of the ankle joints and automatically calculate the pedaling speed. An isokinetic dynamometer was used to assess the isometric and isokinetic peak torques of knee flexion and extension. Results: The pedaling speeds in VR and built-in speedometer modes revealed a significantly high positive correlation (r = 0.922). In addition, the intra-rater reliability of the pedaling speed in the VR speedometer mode was good (ICC [intraclass correlation coefficient] = 0.685). The results of the Pearson correlation analysis revealed a significant moderate positive correlation between the pedaling speed of the VR speedometer and the peak torque of knee isokinetic flexion (r = 0.639) and extension (r = 0.598). Conclusion: This study suggests the potential benefits of measuring the maximum pedaling speed using 3D depth camera in a VR environment as an indirect assessment of muscle strength. However, technological improvements must be followed to obtain more accurate estimation of muscle strength from the VR cycling test.

Genome-wide identification of chemosensitive single nucleotide polymorphism markers in gastric cancer.

Ha, Ye Jin,Yoon, Sang Nam,Jeon, Yeo Jin,Cho, Dong Hyung,Roh, Seon Ae,Kim, Byung Sik,Kim, Hee Jin,Kim, Seon Young,Kim, Yong Sung,Kim, Jin Cheon Potamitis Press 2011 Anticancer research Vol.31 No.12

<P>A chemosensitive single nucleotide polymorphism (SNP) discovery schema is presented that utilizes (i) genome-wide SNP screening, with a human SNP array and an in vitro chemosensitivity assay, in 93 patients with gastric cancer (GC), and (ii) biological utility assessment using cell viability assays of transfected GC cells. Cytotoxicity analysis showed that most of the MKN1 and SNU638 clones transfected with the G allele of Deoxyribonuclease II beta (DNASE2B) rs3738573 were more sensitive to docetaxel than those with the C allele (p0.001-0.029) and most of the AGS and SNU638 clones transfected with the T allele of 5-hydroxytryptamine receptor IE (HTRIE) rs3828741 were more sensitive to paclitaxel than those with the C allele (p0.001-0.019). Our findings show that the two novel markers, DNASE2B rs3738573 and HTR1E rs3828741, have potential for improving the prediction of chemosensitivity of GC patients.</P>

The Huge ovarian mass mimicking a uterine sarcoma

( Ye Jin Seon ),( So Ryeoung Kim ),( Ji Suk Han ),( Hee Young Ahn ),( Eun Jin Heo ),( Yun Dan Kang ),( Jong Soo Kim ),( Jin Wan Park ) 대한산부인과학회 2018 대한산부인과학회 학술대회 Vol.104 No.-

Uterine sarcomas are rare mesodermal tumors that account for 3-7% of uterine cancer. They are a heterogeneous group of tumors and have poor prognosis. It is difficult to diagnosis at preoperatively, that the positive predictive value using image study such as Abdominal Pelvis computed tomography (AP-CT) or Pelvis magnetic resonance (MRI) scan is very low. We present a rare case of the primary ovarian fibrothercoma mimicking uterine sarcoma. A 89-years old postmenopausal women present with progressive abdominal pain and palpable abdominal mass. AP-CT revealed about 16cm sized uterine was suspected uterine sarcoma. Tumor markers are normal range. She underwent exploratory laparotomy. There are a huge irregular hard mass with several feeding vessels originated right ovary and grossly normal uterus and left adnexa. A surgical exploration was performed within whole abdomen. there was no metastatic nodules. we performed Right salpingo-oophorectomy and requested frozen biopsy at pathologist. It was a ovarian stromal tumor. Therefore, we performed total hysterectomy, Left salpingo-oophorectomy and both pelvic lymph node biopsy. The final pathologic diagnosis documented an ovarian fibrothecoma. This case reported an unusual case of the primary ovarian fibrothecoma mimicking uterine sarcoma.

Spontaneous vaginal hemorrhage caused by choriocarcinoma : Necessity for the early diagnosis and treatment

( Ye Jin Seon ),( Ji Hye Kim ),( Yun Dan Kang ),( Jin Wan Park ),( Jong Soo Kim ) 대한산부인과학회 2019 대한산부인과학회 학술대회 Vol.105 No.-

Hemorrhagic choriocarcinoma lesions may lead to life-threatening conditions, but an early diagnosis contributes to a potential cure. We report a case of an woman with choriocarcinoma, that occurred within one month after full term delivery. The 25-year-old patient with gravida 1, para 1 was admitted in our hospital for massive vaginal bleeding and dry cough. Radiological investigation found a 8.4cm mass in enlarged uterus and also multiple nodules in both lungs. Her serum β-hCG level was 747,682 mIU/mL. An emergency surgery was performed to control heavy vaginal bleeding. The patient was early diagnosed as choriocarcinoma FIGO stage III and the score of the Prognostic Scoring Index modified by the WHO was 12. Multiagent chemotherapy, the EMACO regimen(etoposide, actinomycin D, methotrexate, vincristine and cyclophosphamide) was administered. After the seventh chemotherapy session, her serum β-hCG level was <5 mIU/mL. The diagnosis of choriocarcinoma may be difficult, especially in the setting with the limit of medical resources. The early application of various diagnostic techniques such as x-ray, computed tomography is helpful for evaluating the patient´s condition.

Novel analysis procedure for red ginseng polysaccharides by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry

Ye Rin Jin,Myung Jin Oh,Heung Joo Yuk,Hyun Joo An,Dong Seon Kim 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.5

Background: Red ginseng polysaccharides (RGPs) have been acknowledged for their outstanding immunomodulation and anti-tumor activities. However, their studies are still limited by the complexity of their structural features, the absence of purification and enrichment methods, and the rarity of the analytical instruments that apply to the analysis of such macromolecules. Thus, this study is an attempt to establish a new mass spectrometry (MS)-based analysis procedure for RGPs. Methods: Saponin pre-excluded powder of RG (RG-SPEP, 10 mg) was treated with 200 mL of distilled water and centrifuged for 5 h at 1000 rpm and 85 _C. Ethanol-based precipitation and centrifugation were applied to obtain RGPs from the heated extracts. Further, endo-carbohydrase treatments were performed to produce specific saccharide fragments. Solid-phase extraction (SPE) processes were implemented to purify and enrich the enzyme-treated RGPs, while matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) MS was employed for the partial structural analysis of the obtained RGPs. Results: Utilizing cellulase, porous graphitized carbon (PGC), hydrophilic interaction chromatography (HILIC), and MALDI-TOF/TOF MS, the neutral and acidic RGPs were qualitatively analyzed. Hexn and Hexn-18 (cellulose analogs) were determined to be novel neutral RGPs. Additionally, the [Unknown + Hexn] species were also determined as new acidic RGPs. Furthermore, HexAn (H) was determined as another form of the acidic RGPs. Conclusion: Compared to the previous methods of analysis, these unprecedented applications of HILICSPE and MALDI-TOF/TOF MS to analyze RGPs proved to be fairly effective for fractionating and detecting neutral and acidic components. This new procedure exhibits great potential as a specific tool for searching and determining various polysaccharides in many herbal medicines.

Biodegradation of Polyethylene by Pseudomonas alcaligene KY1214 Was Detected through FTIR and SEM Analysis

Ye-Jin KIM,Sol-Hee LEE,Hak-Seon KIM,Hyun-Ouk KIM,Suk-Jin HA 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.4

Neuroendocrine Differentiation in Acquired Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor

( You Jin Chang ),( Seon Ye Kim ),( Yun Jung Choi ),( Kwang Sup So ),( Jin Kyung Rho ),( Woo Sung Kim ),( Jae Cheol Lee ),( Jin Haeng Chung ),( Chang Min Choi ) 대한결핵 및 호흡기학회 2013 Tuberculosis and Respiratory Diseases Vol.75 No.3

Background:Smallcelllungcancer(SCLC)transformationduringepidermalgrowthfactorreceptortyrosinekinaseinhibitor(EGFR-TKI)treatmentinlungcancerhasbeensuggestedasoneofpossibleresistancemechanisms.Methods:WeevaluatedwhetherSCLCtransformationorneuroendocrine(NE)differentiationcanbefoundinthecelllinemodel.Inaddition,wealsoinvestigateditseffectonresponsestoconventionalchemotherapeuticdrugsoftheSCLCtreatment.Results:ResistantcelllinestovariouskindsofEGFR-TKIssuchasgefitinib,erlotinib,CL-387,785andZD6474withA549,PC-9andHCC827lungadenocarcinomacelllineswereestablished.Amongthem,tworesistantcelllines,A549/GR(resistanttogefitinib)andPC-9/ZDR(resistanttoZD6474)showedincreasedexpressionsofCD56whileincreasedsynaptophysin,Rb,p16andpoly(ADP-ribose)polymerasewerefoundonlyinA549/GRinwesternblotting,suggestingthatNEdifferentiationoccurredinA549/GR.A549/GRcellsweremoresensitivetoetoposideandcisplatin,chemotherapeuticdrugsforSCLC,comparedtoparentalcells.TreatmentwithcAMPandIBMXinducedsynaptophysinandchromograninAexpressioninA549cells,whichalsomadethemmoresensitivetoetoposideandcisplatinthanparentalcells.Furthermore,wefoundatissuesamplefromapatientwhichshowedincreasedexpressionsofCD56andsynaptophysinafterdevelopmentofresistancetoerlotinib.Conclusion:NEdifferentiationcanoccurduringacquisitionofresistancetoEGFR-TKI,leadingtoincreasedchemosensitivity.

Colonization of Beauveria bassiana ERL836 on damaged logs of pine tree reduced emergences of Japanese pine sawyer beetles

Jeong Seon Yu,So Eun Park,Ye Ram Im,In Soo Jeon,Yu Lim Park,Yu Jin Jeong,Sue Yeon Lee,Woo Jin Kim,Se Jin Lee,Jae Su Kim 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.5

불소도포 및 지각과민처치제 적용 후 상아세관 폐쇄효과와 지속력 평가

이예진 ( Ye-jin Lee ),정문진 ( Moon-jin Jeong ),안용순 ( Yong-soon Ahn ),천수경 ( Su-kyung Cheun ),정순정 ( Soon-jeong Jeong ),임도선 ( Do-seon Lim ) 한국치위생과학회 2016 치위생과학회지 Vol.16 No.4

본 연구는 치과병·의원에서 일반적으로 사용되고 있는 불소화합물과 지각과민처치제의 상아세관 폐쇄효과를 비교분석하고, 전동칫솔질을 시행하여 시간경과에 따른 유효성 분의 지속력을 평가하고자 하였다. 발거된 지 3개월 이내의 우식이나 수복, 보철물이 없는 건전한 사람의 대구치 35개를 선택하여 4등분하였고 이 중 135개의 시편을 본 연구에 사용하였다. 이들 시편을 아무런 처치를 하지 않은 대조군과 5개의 실험군으로 구분하였고, 실험군은 산성불화인산염, 불소바니쉬, Gluma, Super Seal, SE-Bond를 선정하여 대상치아에 도포하였다. 이후 1주(140회), 2주(280회), 4주 (560회)에 해당하는 전동칫솔질을 시행하였고, 각 시편별 3 부위를 주사전자현미경(SEM)으로 상아세관 폐쇄도를 관찰하였다. 그 결과 전동칫솔질 시행 1주, 2주, 4주에서 모두 불소바니쉬 도포군의 상아세관 폐쇄도가 가장 높았으며, 그 다음으로 SE-Bond 도포군이 높게 나타났다. 또한 Gluma 도포군은 다른 실험군에 비해 가장 낮은 상아세관 폐쇄도를 나타냈으며, 칫솔질 시행 4주에서는 Gluma 도포군과 Super Seal 도포군이 다른 실험군에 비해 낮은 상아세관 폐쇄도를 나타냈다. 본 연구를 종합하면, 처치 4주 후에도 불소바니쉬와 SE-Bond는 다른 실험군에 비해 상아세관 폐쇄도가 높은 것으로 나타났다. 따라서 임상에서 지각과민 치료 시 불소바니쉬와 SE-Bond의 사용이 효과적일 것으로 생각된다 This study compared and analyzed the occluding effects of fluoride compounds and desensitizers, which are commonly used in dental clinics, on dentinal tubules. This study also evaluated the persistence of the active ingredients over time by performing toothbrushing with an electric toothbrush. Thirty-five molar teeth, which had been extracted within the past 3 months from healthy people without tooth decays, amalgam fillings, or dental crowns, were divided into 4 pieces each. Of these, 135 teeth pieces were used as study specimens. These specimens were divided into a control group, an untreated group, and 5 experimental groups (acidulated fluoride gel, fluoride varnish, Gluma, Super Seal, and SE-Bond). The specimens were then subjected to toothbrushing equivalent to 1 week (140 times), 2 weeks (280 times), and 4 weeks (560 times), and the occluding effects on dentinal tubules in 3 regions of each specimen were examined under a scanning electron microscope. The fluoride varnish treated group showed the highest degree of dentinal tubule occlusion effects during the first, second, and fourth weeks of toothbrushing, with the SE-Bond treated group showing the second highest degree and the Gluma treated group showing the lowest degree. After 4 weeks of toothbrushing, the Gluma treated group and the Super Seal treated group showed the lowest degrees of dentinal tubule occlusion effects. In summary, the fluoride varnish treated group and the SE-Bond treated group displayed higher occlusion effects even after 4 weeks of treatment than did the other experimental groups. Therefore, it is the authors’ belief that fluoride varnish and SE-Bond are effective for treating dentinal hyperesthesia.