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홍용,김현식,고유곤,홍동표,김성욱 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-
In this paper, gear system for Micro-press actuator parts is developed. The micro-press system consists of AC geared servo motor, gear system and high precision ball screw. Herein, the micro-press gear system not only forms the micro thin foil valve but also forms the hydro dynamic bearing for a high precision rotational equipment, we analyzed the transmitted power capacity of gear system and torque, angular velocity of ball screw, because we are to select the appropriate servo motor and then we apply result of analysis to the micro press system. For the next step, we plan to study for the development of forming speed for a productivity with high precision.
홍용,김현식,고유곤,홍동표,김성욱 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-
In this paper, gear system for Micro-press actuator parts is developed. The micro-press system consists of AC geared servo motor, gear system and high precision ball screw. Herein, the micro-press gear system not only forms the micro thin foil valve but also forms the hydro dynamic bearing for a high precision rotational equipment. we analyzed the transmitted power capacity of gear system and torque, angular velocity of ball screw, because we are to select the appropriate servo motor and then we apply result of analysis to the micro press system. For the next step, we plan to study for the development of forming speed for a productivity with high precision.
朴映淳,朴鍾君,金賢玉,金鍾鎬,李東燁,金完柱 圓光大學校 1988 論文集 Vol.22 No.2
培養鷄胚筋細胸 分化에 미치는 促進物質의 영향을 알아보고자 2가양이온 (Ca2+, Mg2+) 胸胚抽出物 (EE), polyethylene glycol (PEG), 再生中인 도마뱀 (Lacertilia)꼬리 추출물 (BE)을 처리하여 細胸融合指數를 비교 조사하였다. 정상배양시의 細胸融合指數는 배양시작 70 시간에 최대에 달했으며 더이상 증가하지 않았고, Ca2+와 Mg2+ 처리할 경우에는 농도가 증가할수록 細胸融合指數는 저하되고, 근세포의 굵기도 정상세포보다 가늘고 핵이 모여있었다. Polyethylene Glycol (PEG)와 Blastemata Extract (BE) 처리군에서는 대조구와 큰 차이가 없는 것으로 나타났다. 鷄胚抽出物 (EE) 을 筋細胞分化에 필수적이나 과량으로 添加시에는 細胞融合은 억제되고 세포수는 증가되는것으로 나타났다. To study the effects of promoting factors on the differentiation of muscle cells, chick embryonic myoblasts were cultured with divalent cations (Ca2+, Mg2+) Chick embryo extract (EE), Polyethylene Glycol (PEG) and Blastema extract of Lacertilia (BE). Fusion index and Growth ratio were measured at various culture time. The fusion index of normal myoblast culture was reached maximum at 70 hours and the divalent cations (Ca2+, Mg2+) treated groups were decreased at higher concentration. The cellular formation of myoblasts treated with divalent cations was thiner and more aggregated between nuclei than that of normal myoblasts calture was. When polyethylene glycol (PEG) and Blastema extract of Lacertilia (BE) were treated to the myoblasts, tha fusion indices were similar to the control. Embryo Extract (EE) was seemed to be necessary for the fusion of chick embryonic myoblasts in culture, however, when excess of EE was added the fusion index was decreased and the number of cells was increased.
Kim, Heonjo,Chae, Youngjoo,Lee, Duck Hyun,Kim, Minsik,Huh, Jiyoung,Kim, Youngki,Kim, Hyunjin,Kim, Hyun Jung,Kim, Sang Ouk,Baik, Hionsuck,Choi, Kihang,Kim, Jong Seung,Yi, Gi-Ra,Lee, Kwangyeol WILEY-VCH Verlag 2010 Angewandte Chemie Vol.49 No.33
<B>Graphic Abstract</B> <P>Playing a dual role as a catalyst that destabilizes Fe nanoparticles to form soluble precursors in situ and as a catalytic center for nanorod growth allows Pd nanoparticles to transform Fe nanoparticles and a P source into Fe<SUB>2</SUB>P nanorods (see scheme). The diameter and length of the Fe<SUB>2</SUB>P nanorods can be fine-tuned by means of the diameter of the Pd nanoparticles and the Fe/Pd ratio, respectively. <img src='wiley_img_2010/14337851-2010-49-33-ANIE201001822-content.gif' alt='wiley_img_2010/14337851-2010-49-33-ANIE201001822-content'> </P>
점증부하 최대운동이 운동 전 후 혈중지질에 미치는 영향
김성수,현정화,신말순,김영표,이삼준,권순욱,이재현,김상호 대한스포츠의학회 1996 대한스포츠의학회지 Vol.14 No.1
This study was designed to investigate the effects of graded maximal exercises on serum lipids. The subjects consisted of 7 athletes group(A group) and 7 non-athlete group(B group) and who all were about twenty-one years old. Their blood samples were collected at pre and post(after exhaustion). The collected blood were analyzed for the triglyceride, cholesterol, High density lipoprotein cholesterol and TC/HDL-C ratio, Apo A, B. The result through the statistical analysis of this data were summarized as follows; 1. Triglyceride, total cholesterol, LDL-C, TC/HDL-C ratio level, athlete groups were higher than those in non-athlete groups after maximal exercise. There were no significant differences between two groups. 2. HDL-C level was decreased after maximal exercise in athlete groups but was increased in non-athlete groups. There were significant differences between two groups. 3. Apo A level was increased after maximal exercise in two groups, especially athlete groups were higher than those in non-athlete groups after maximal exercise. There were significant differences between two groups. 4. Apo B were no significant difference between two groups
Anchored protease-activatable polymersomes for molecular diagnostics of metastatic cancer cells
Kim, Hyun-Ouk,Lim, Jong-Woo,Choi, Jihye,Lee, Hwunjae,Son, Hye Young,Kim, Jihye,Park, Geunseon,Chun, Haejin,Song, Daesub,Huh, Yong-Min,Haam, Seungjoo Royal Society of Chemistry 2017 Journal of Materials Chemistry B Vol. No.
<P>Real-time quantitative and qualitative analyses of metastasis-associated proteases are critical for precise diagnosis and novel therapeutic treatment of advanced cancers. However, conventional methods based on DNA, peptides, and proteins require sophisticated chemistry and additional processes to expose detection moieties, and they lack elements of temporal control, which limit their applicability. We designed unique protease-activatable polymersomes (PeptiSomes) for high sensitivity,<I>in situ</I>quantitative analysis of activating membrane-type 1 matrix metalloproteinases (MT1-MMP, MMP14). To do this, we first synthesized an amphiphilic block polymer-peptide and a copolypeptide based on mPEG-<I>b</I>-pLeu and MT1-peptide-<I>b</I>-pLeu, respectively. Amphiphilic self-assembled PeptiSomes in water were capable of disassembling and releasing the encapsulated self-quenched fluorescence dye (calcein)<I>via</I>enzymatic activation by MT1-MMP. Our PeptiSome system may potentially prevent the initiation and progression of cancer metastasis. Furthermore, the PeptiSome approach described here is likely to facilitate the development of rapid protease assay techniques and further extend the role of proteases as metastasis indicators and therapeutic targets.</P>
Kim, Hong-Sik,Kang, Beom-Kyu,Seo, Jeong-Hyun,Ha, Tae-Joung,Kim, Hyun-Tae,Shin, Sang-Ouk,Park, Chang-Hwan,Kwak, Do-Yeon The Korean Society of Crop Science 2019 한국작물학회지 Vol.64 No.2
The variation of content of 12 soybean seed isoflavone components was determined in the aglycone, glucoside, malonylglucoside and acetylglucoside groups of 44 Korean soybean cultivars grown in 2016 as well as in 2017. The total isoflavone content of the 44 cultivars averaged at $2935.4{\mu}g/g$ and was in the range of 950.6 to $5226.3{\mu}g/g$ for two years. Malonylglucoside group averaged at $2437.2{\mu}g/g$ with the highest proportion of isoflavone composition (83.0%). Significant differences were observed between cultivars, years and their interactions for both the total isoflavone and each composition group contents (P < 0.0001); however, no year-wise differences were observed for daidzein and genistin. The broad-sense heritability ($h^2$) within the set of 44 Korean soybean cultivars was as high as 0.93 for the total isoflavone content and was in the range of 0.8-0.92 for each composition group of isoflavone except for acetylglucoside. The total isoflavone content in cultivar group for soy-sprout was higher ($3850.4{\mu}g/g$) than that for the other cultivar groups of soy-paste and tofu ($3082.8{\mu}g/g$), black or green soybean cooked with rice ($2345.8{\mu}g/g$), and early maturity group ($1298.6{\mu}g/g$). The total isoflavone content of 'Sowonkong', a soybean cultivar for soy-sprout, was the highest ($5226.3{\mu}g/g$). In the cultivar group for soy-paste and tofu, the average isoflavone contents of 'Daepung', 'Daepung2ho', 'Saegeum', 'Uram', and 'Jinpung' were higher than $4000{\mu}g/g$. With the exception of small seeded cultivars with low isoflavone contents such as 'Sohwang' and 'Socheongja', the seed size and total isoflavone content were significantly negatively correlated in 2016 and 2017, respectively ($r=-0.47^{**}$ and $-0.49^{**}$). The number of days of growth from flowering to maturity did not affect the variations observed in isoflavone content.
Dengue virus-Polymersome Hybrid nanovesicles for Real-Time Single Virus Tracking
( Hyun-ouk Kim ),( Woonsung Na ),( Minjoo Yeom ),( Seungjoo Haam ),( Daesub Song ) 대한인수공통전염병학회 2019 창립총회 및 학술대회 초록집 Vol.2019 No.1
Introduction: Dengue virus is one of the major infectious human pathogens worldwide. Currently, no antiviral drug has become available against the dengue virus induced diseases since little is known regarding how dengue virus interacts with host cells. Dengue virus-polymersome hybrid nanovesicles are powerful tools to explain the dynamics of host cell-virus interaction and tracking. Methods: Dengue virus-polymersome hybrid nanovesicles were prepared by dialysis method. We showed that DiD and BODIPY FL C5-Ceramide encapsulated dengue virus-polymersome hybrid nanovesicles triggered the formation of red and green fluorescence by using real-time fluorescence microscopy. The internalization to escape endosomal entrapment was determined by confocal laser scanning microscopy. Results: Fluorescence images of DiD and BODIPY FL C5-Ceramide encapsulated dengue virus-polymersome hybrid nanovesicles illustrated at different time points. For real-time virus tracking study, it is desirable to acquire images to obtain adequately high resolution to monitor the dynamics of host cell-virus interaction and tracking in living cells. Conclusion: This study exploited a virus-nanovesicles tracking technology to approach whether dengue virus interacts with autophagy. Therefore, we demonstrated that dengue virus-polymersome hybrid nanovesicles will be utilized for virus tracking studies to examine the mechanisms of viral infections.