LPL gene Pvu II polymorphism and hypertriglycer-idemia: a meta-analysis involving 1,640 subjects

( Yan-yan Li ),( Yan-hong Zhou ),( Ge Gong ),( Hong-yu Geng ),( Xin-xing Yang ),( Xiang-ming Wang ),( Chuan-wei Zhou ),( Jian Xu ),( Yun Qian ) 대한내과학회 2017 The Korean Journal of Internal Medicine Vol.32 No.6

Background/Aims: Although lipoprotein lipase (LPL) gene Pvu II polymorphism has been associated with an increased risk of hypertriglyceridemia (HT), there is no clear consensus within the scientific community. Methods: A meta-analysis of 1,640 subjects from six individual studies was conducted to better elucidate the potential relationship between the LPL gene Pvu II polymorphism and HT within the Chinese population. Pooled odds ratios (ORs) and their corresponding 95% confidence intervals (CIs) were evaluated by using fixed effect models. Results: Our analysis indicated a significant association between LPL gene Pvu II polymorphism and HT within the Chinese population under allelic (OR, 1.550; 95% CI, 1.320 to 1.830; p = 1.158 × 10^-7), recessive (OR, 0.540; 95% CI, 0.390 to 0.750; p = 0.0002), dominant (OR, 1.889; 95% CI, 1.501 to 2.377; p = 5.960 × 10^-8), homozygous (OR, 2.167; 95% CI, 1.531 to 3.067; p = 1.242 × 10^-5), heterozygous (OR, 1.810; 95% CI, 1.419 to 2.309; p = 1.842 × 10^-6), and additive genetic models (OR, 1.553; 95% CI, 1.320 to 1.828; p = 1.158 × 10^-7). Conclusions: Because LPL gene Pvu II restriction fragment length polymorphism polymorphism was associated with an elevated risk of HT, the P+ allele carriers of the LPL gene might be predisposed to HT.

Preparation of carbonated apatite nano-whiskers in SBF with ultrasonic assistance

Yanli Geng,Yanbao Li,Dongxu Li 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.5

Carbonated apatite nano-whiskers were prepared in simulated body fluids (SBF) with ultrasonic assistance at 37℃. The effects of the initial pH value in the SBF solution and reaction times were investigated in this study. The calcium phosphate precipitates were examined by XRD, FTIR and TEM techniques. The results showed that the initial pH value in the SBF had an important effect on the morphology and composition of the calcium phosphate precipitates, and carbonated apatite nanowhiskers with length/diameter ratio of ~20 were obtained at an initial pH = 7.5 after ultrasonic treatment for 24 h. Carbonated apatite nano-whiskers were prepared in simulated body fluids (SBF) with ultrasonic assistance at 37℃. The effects of the initial pH value in the SBF solution and reaction times were investigated in this study. The calcium phosphate precipitates were examined by XRD, FTIR and TEM techniques. The results showed that the initial pH value in the SBF had an important effect on the morphology and composition of the calcium phosphate precipitates, and carbonated apatite nanowhiskers with length/diameter ratio of ~20 were obtained at an initial pH = 7.5 after ultrasonic treatment for 24 h.

p₂₂phox C₂₄₂T gene polymorphism and overt diabetic nephropathy: a meta-analysis of 1,452 participants

( Yan-yan Li ),( Ge Gong ),( Hong-yu Geng ),( Yun Qian ) 대한내과학회 2017 The Korean Journal of Internal Medicine Vol.32 No.6

Background/Aims: The p22phox C242T gene polymorphism (rs4673) may be linked to an increased susceptibility for overt diabetic nephropathy (ODN), but the study results are still inconclusive. Methods: To explore the relationship between p22phox C242T gene polymorphism and ODN, the current meta-analysis of 707 ODN patients and 745 controls from five individual studies was conducted. The pooled odds ratio (OR) and its corresponding 95% confidence interval (CI) were evaluated by either a random or fixed effect model. Results: In our meta-analysis, a significant relationship between the p22phox C242T gene polymorphism and ODN was found under allelic (OR, 2.760; 95% CI, 1.400 to 5.450; p = 0.004), recessive (OR, 5.080; 95% CI, 1.020 to 25.430; p = 0.05), dominant (OR, 1.700; 95% CI, 1.167 to 2.477; p = 0.006), homozygous (OR, 3.900; 95% CI, 1.022 to 14.889; p = 0.046), heterozygous (OR, 1.523; 95% CI, 1.167 to 1.986; p = 0.002), and additive genetic models (OR, 2.019; 95% CI, 1.232 to 3.309; p = 0.005). Conclusions: A positive correlation between p22phox C242T gene polymorphism and ODN risk was found. The T allele carriers of p22phox C242T gene polymorphism might be predisposed to ODN.

Early-age thermal analysis and strain monitoring of massive concrete structures

Yan Geng,Xiongyan Li,Suduo Xue,Jinguang Li,Yanjie Song 사단법인 한국계산역학회 2018 Computers and Concrete, An International Journal Vol.21 No.3

Hydration heat and thermal induced cracking have always been a fatal problem for massive concrete structures. In order to study a massive reinforced concrete wall of a storage tank for liquefied natural gas (LNG) during its construction, two mock-ups of 0.8 m×0.8 m×0.8 m without and with metal corrugated pipes were designed based on the actual wall construction plan. Temperature distribution and strain development of both mock-ups were measured and compared inside and on the surface of them. Meanwhile, time-dependent thermal and mechanical properties of the concrete were tested standardly and introduced into the finite-element (FE) software with a proposed hydration degree model. According to the comparison results, the FE simulation of temperature field agreed well with the measured data. Besides, the maximum temperature rise was slightly higher and the shrinkage was generally larger in the mock-up without pipes, indicating that corrugated pipes could reduce concrete temperature and decrease shrinkage of surrounding concrete. In addition, the cooling rate decreased approximately linearly with the reduction of heat transfer coefficient h, implying that a target cooling curve can be achieved by calculating a desired coefficient h. Moreover, the maximum cooling rate did not necessarily decrease with the extension of demoulding time. It is better to remove the formwork at least after 116 hours after concrete casting, which promises lower risk of thermal cracking of early-age concrete.

The effect of a parabolic potential on the properties of a strongly coupled polaron in an asymmetric Gaussian quantum well

Geng Yan-Bo,Ding Zhao-Hua,Xiao Jing-Lin 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.79 No.1

In this work, the effect of parabolic potential on the properties of strongly coupled polaron in an asymmetric Gaussian quantum well (QW) has been studied. Relations of the frequency vibration and the strongly coupled polaron’s ground-state energy in an asymmetric Gaussian QW for RbCl crystals at different confinement strengths along the x and the y directions, the asymmetric Gaussian QW barrier heights, and the Gaussian confinement potential widths and temperatures have been studied using the Lee–Low–Pines unitary transformation and the linear combination operator methods. Calculations show that the absolute value of ground-state energy increased with increasing confinement strengths along the x and the y directions and asymmetric Gaussian QW barrier height, and derease with increeasing Gaussian confinement potential width, and temperature. In addition, vibrational frequency was found to decrease with increasing Gaussian confinement potential width and to increase with increasing confinement strengths along the x and the y directions, asymmetric Gaussian QW barrier height, and temperature. The five parameters involved in this paper are important physical quantities for studying the properties of Gaussian QWs, and have important physical significance for the study of nanomaterials.

Forecasting Logistics Demand Using Unbiased GM (1,1) Model Optimized by AIWPSO Algorithm

Li-Yan Geng,Zhan-Fu Zhang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.10

Accurate forecast of logistics demand can provide scientific guidance for logistics planning and decision making. With the complexity and uncertainty characteristics in logistics demand, the forecasting of logistics demand shows comprehensive and complex. The forecasting precision of the traditional forecasting methods often are not satisfying. It is necessary to look for novel forecasting methods to enhance the forecasting precision of logistics demand. Integrating the unbiased GM (1,1) model (UGM (1,1)) into the adaptive inertia weight particle swarm optimization (AIWPSO) algorithm, this paper developed a novel model for forecasting logistics demand, called AIWPSO-UGM (1,1) model, in which the UGM (1,1) model was used to forecast logistics demand and the AIWPSO algorithm was adopted to optimize the grey parameters needed in UGM (1,1) model. Two examples were selected to prove the out-of-sample performance of the AIWPSO-UGM (1,1) model in forecasting logistics demand. The results imply that the proposed AIWPSO-UGM (1,1) model performs better in logistics demand forecasting compared to the GM (1,1) model optimized by AIWPSO algorithm (AIWPSO-GM (1,1)), UGM (1,1), and GM (1,1) models.

Molecular Cloning, Segmental Distribution and Ontogenetic Regulation of Cationic Amino Acid Transporter 2 in Pigs

Zou, Shi-geng,Zhi, Ai-min,Zhou, Xiang-yan,Zuo, Jian-jun,Zhang, Yan,Huang, Zhi-yi,Xu, Ping-Wen,Feng, Ding-yuan Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.5

The goal of this study was to elucidate the expression and segmental distribution of the glomerular cationic amino acid metabolism transporter-2 (CAT-2) and thus to improve our understanding of porcine cationic amino acid transporters and amino acid absorption. Porcine CAT-2 was cloned, sequenced and characterized. The predicted amino acid sequence of porcine CAT-2 shared 86.1% and 92.1% identity with human and mouse CAT-2A, respectively. The tissue distribution patterns and ontogenic changes of CAT-2 mRNAs were determined by real-time Q-PCR. The results showed that porcine CAT-2 was highly expressed in the heart and intestinal tract (duodenum, ileum and jejunum). In addition, the mRNA of CAT-2 was found in liver, lung, kidney, brain and muscle. Within the intestinal tract, CAT-2 mRNA was most abundant in the ileum and rarely expressed in the duodenum. In the duodenum, the levels of CAT-2 mRNA reached their peak on day 7 (p<0.05) while in the jejunum, levels were low on day 1 and 7 and increased rapidly after day 26 before peaking on days 30 and 60 (p<0.05). The levels then dramatically decreased by day 90 (p<0.05). In the ileum, levels achieved their maximum on day 30 and then decreased significantly on day 60 (p<0.05).

Comparative Genomics Uncovers the Genetic Diversity and Synthetic Biology of Secondary Metabolite Production of Trametes

( Yan Zhang ),( Jingjing Wang ),( Chen Yajun ),( Minghui Zhou ),( Wei Wang ),( Ming Geng ),( Decong Xu ),( Zhongdong Xu ) 한국균학회 2020 Mycobiology Vol.48 No.2

The carbohydrate-active enzyme (CAZyme) genes of Trametes contribute to polysaccharide degradation. However, the comprehensive analysis of the composition of CAZymes and the biosynthetic gene clusters (BGCs) of Trametes remain unclear. Here, we conducted comparative analysis, detected the CAZyme genes, and predicted the BGCs for nine Trametes strains. Among the 82,053 homologous clusters obtained for Trametes, we identified 8518 core genes, 60,441 accessory genes, and 13,094 specific genes. A large proportion of CAZyme genes were cataloged into glycoside hydrolases, glycosyltransferases, and carbohydrate esterases. The predicted BGCs of Trametes were divided into six strategies, and the nine Trametes strains harbored 47.78 BGCs on average. Our study revealed that Trametes exhibits an open pan-genome structure. These findings provide insights into the genetic diversity and explored the synthetic biology of secondary metabolite production for Trametes.

Preparation and Characterization of Paclitaxel-Loaded PLGA Nanoparticles Coated with Cationic SM5-1 Single-chain Antibody

( Geng Kou ),( Jie Gao ),( Hao Wang ),( Huai Wen Chen ),( Bo Hua Li ),( Da Peng Zhang ),( Shu Hui Wang ),( Sheng Hou ),( Wei Zhu Qian ),( Jian Xin Dai ),( Yan Qiang Zhong ),( Ya Jun Guo ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.5

The clinical performance of APTIMA human papillomavirus and Hybrid Capture 2 assays in the triage of lesser abnormal cervical cytologies

Yanli Guo,Ke You,Li Geng,Jie Qiao 대한부인종양학회 2014 Journal of Gynecologic Oncology Vol.25 No.4

Objective: This study was performed to evaluate the clinical performance of APTIMA human papillomavirus (AHPV) assay and Hybrid Capture 2 (HC2) assay in screening for cervical disease, especially in women with atypical squamous cell of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL). Methods: A total of 411 women diagnosed with ASC-US or LSIL were referred and further triaged by HC2 test. Prior to colposcopy, liquid-based cytology specimens were collected for the AHPV assay. Sensitivity and specificity were established based on the histological findings of cervical intraepithelial neoplasia (CIN). Results: In all 411 subjects, the positive detection rate of AHPV assay was 70.8% (95% confidence interval [CI], 66.4 to 75.2), which was significantly lower than the positive detection rate of 94.9% obtained using HC2 test (95% CI, 92.3 to 96.8). Only one CIN 3-positive case was detected among the 120 AHPV-negative women, which was then confirmed by Pap smear test to be LSIL. The sensitivities of AHPV and HC2 for CIN 3 were similar (94.1% and 100%, respectively). However, AHPV showed a significantly higher specificity than HC2 test (30.2% and 5.3%, respectively; p<0.001). Conclusion: AHPV assay is effective in identifying CIN 3-positive cases because of its high specificity and lower false-negative rate. The use of AHPV for the triage of ASC-US and LSIL might help to reduce the referral rate of colposcopy during cervical cancer screening.