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( Zhi-qiang Ren ),( Yan Liu ),( Xiao-qiong Pei ),( Zhong-liu Wu ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.11
Ethyl (S)-3-hydroxy-3-(2-thienyl) propanoate ((S)-HEES) acts as a key chiral intermediate for the blockbuster antidepressant drug duloxetine, which can be achieved via the stereoselective bioreduction of ethyl 3-oxo-3-(2-thienyl) propanoate (KEES) that contains a 3-oxoacyl structure. The sequences of the short-chain dehydrogenase/reductases from Chryseobacterium sp. CA49 were analyzed, and the putative 3-oxoacyl-acyl-carrier-protein reductase, ChKRED12, was able to stereoselectively catalyze the NADPH-dependent reduction to produce (S)-HEES. The reductase activity of ChKRED12 towards other substrates with 3- oxoacyl structure were confirmed with excellent stereoselectivity (>99% enantiomeric excess) in most cases. When coupled with a cofactor recycling system using glucose dehydrogenase, the ChKRED12 was able to catalyze the complete conversion of 100 g/l KEES within 12 h, yielding the enantiopure product with >99% ee, showing a remarkable potential to produce (S)-HEES.
Hai-Yan Yu,Zhi-Feng Zhou,Jun-Qiang Jia,Zhong-Zheng Gui 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
Delta 6-fatty acid desaturase is a membrane-bound enzyme, which is the rate-limiting factor in the biosynthesis of polyunsaturated fatty acids. In this study, a novel delta 6-desaturase gene was cloned from Bombyx mori (BmD6DES). Sequencing analysis revealed that BmD6DES has an open reading frame of 1357 bp that encodes 448 amino acids. Heterologous expression in yeast demonstrated that BmD6DES could synthesize γ-linolenic acid (GLA, 18:3, Δ6,9,12) by utilizing the endogenous substrate linoleic acid (LA, 18:2, Δ9,12). We found that BmD6DES transcripts were distributed in almost all B. mori tissues, with high expression levels observed at the 5th instar larval, pupal, and adult moth stages. A functional analysis of BmD6DES was performed by measuring mRNA levels after temperature stress, fungal infection, and RNA interference (RNAi). The results indicated that the highest expression of BmD6DES was observed at low temperatures (0 °C) and 6 h to 36 h after fungal infection. qPCR analysis demonstrated that BmD6DES mRNA levels in pupa after BmD6DES RNAi treatment were significantly reduced from 12 h to 72 h compared to those in the control group. Our findings suggest that BmD6DES not only induces the formation of the third carbon–carbon double bond in the LA carbon chain, but also leads to sensitivity to low-temperature stress and fungal infection. These results imply that BmD6DES is a key gene in the γ-linolenic acid pathway during B. mori development.
Zhi-PingWu,Yan-Yan Liu,Guo-Qiang Chen,Ting-LiangWang,Jian-Zhong Tan 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.1
The silkworm fat body is the site of many intermediary metabolic processes, and a source of sustenance forgrowth throughout the life cycle. Fat body proteins are responsible for storing nutrients, providing energy, andregulating hormones, and they have been identified using proteomic approaches. However, detailed differentialexpression of sex-related fat body proteins has not previously been evaluated. In the present study, we characterizedthe differential expression of sex-related fat body proteins, by using 2-dimensional gel electrophoresis(2-DE) followed bymass spectrometry identification and bioinformaticsmethods.We extracted the fat body proteinsfrom 5-day-old fifth instar larvae (L5), 10-day-old fifth instar larvae (corresponding to the end of spinning[LE]), and 0-day-old pupae (P0) of the multivoltine silkworm variety “Da Zao”. We confirmed the presence of 11important sex-specific expression proteins and 14 stage-specific expression proteins.We accurately identified 13of these specific expression proteins, including actin, calponin-like protein, 75 kDa subunit NADH, receptor foractivated protein kinase C from Bombyx mori (BmRACK), IMP (inosine monophosphate) cyclohydrolase, tropomyosin1, β-tubulin, hypothetical protein, antichymotrypsin precursor, and 30 K protein precursor.We showedthat BmRACK was differentially expressed betweenmale and female silkworms.Wediscuss the biological roles ofthe specific expression proteins during the larval–pupal developmental stages.
Dihydroartemisinin inhibits HepG2.2.15 proliferation by inducing cellular senescence and autophagy
( Jiang Zou ),( Qiang Ma ),( Ru Sun ),( Jiajing Cai ),( Hebin Liao ),( Lei Xu ),( Jingruo Xia ),( Guangcheng Huang ),( Lihua Yao ),( Yan Cai ),( Xiaowu Zhong ),( Xiaolan Guo ) 생화학분자생물학회(구 한국생화학분자생물학회) 2019 BMB Reports Vol.52 No.8
Dihydroartemisinin (DHA) has been reported to possess anti-cancer activity against many cancers. However, the pharmacologic effect of DHA on HBV-positive hepatocellular carcinoma (HCC) remains unknown. Thus, the objective of the present study was to determine whether DHA could inhibit the proliferation of HepG2.2.15 cells and uncover the underlying mechanisms involved in the effect of DHA on HepG2.2.15 cells. We found that DHA effectively inhibited HepG2.2.15 HCC cell proliferation both in vivo and in vitro. DHA also reduced the migration and tumorigenicity capacity of HepG2.2.15 cells. Regarding the underlying mechanisms, results showed that DHA induced cellular senescence by up-regulating expression levels of proteins such as p-ATM, p-ATR, γ-H<sub>2</sub>AX, P53, and P21 involved in DNA damage response. DHA also induced autophagy (green LC3 puncta gathered together and LC3II/LC3I ratio increased through AKT-mTOR pathway suppression). Results also revealed that DHA-induced autophagy was not linked to senescence or cell death. TPP1 (telomere shelterin) overexpression could not rescue DHA-induced anticancer activity (cell proliferation). Moreover, DHA down-regulated TPP1 expression. Gene knockdown of TPP1 caused similar phenotypes and mechanisms as DHA induced phenotypes and mechanisms in HepG2.2.15 cells. These results demonstrate that DHA might inhibit HepG2.2.15 cells proliferation through inducing cellular senescence and autophagy. [BMB Reports 2019; 52(8): 520-525]
Ru-Jian Ma,Chun-Yan Wang,Yan-Wei Liu,Thasma Raman Sivakumar,Zi-Xu Ren,Ying Fang,Jun-Qiang Jia,Zhong-Zheng Gui 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.1
While screening for cellulase-producing fungi from insect gut, a fungus with high endoglucanase (carboxymethylcellulase; CMCase) activitywas isolated fromthe larval gut of Bombyx mori. Based onmorphological characteristicsand using an 18S rRNA-based molecular phylogenetic approach, the fungus, strain BMC-2, was identified as aMucor sp. expressing a novel alkalotolerant cellulase. The maximum production of cellulase by the BMC-2 strainwas observed at 55°C and pH8.0. The CMCase activity was inhibited by Cu2+ N Na+ N Zn2+ N Mg2+ N Ba2+, andinduced by Ca2+, Mn2+, Fe2+, and K+.
Performances of the Effervescent Atomizer for Water Mist Fire Extinguishing
( Jin Xiang Wu ),( Qiang Liang ),( Lian Sheng Liu ),( Yun Zhong Yan ),( En Yu Wang ),( Qing Miao ) 한국액체미립화학회 2005 한국액체미립화학회 학술강연회 논문집 Vol.2005 No.-
Based on the mechanism of effervescent atomization, a new effervescent atomization system of water mist was designed. In this system, the gas medium is carbon dioxide, which is soluble and can be used in fire suppression. High pressure saturated carbon dioxide-water solution flowed through the throttle orifice, carbon dioxide was bubbled, and the two-phase flow was formed in the mixing chamber of nozzle. At last, the water mist was formed downstream of the exit orifice of nozzle. In order to examine the feasibility of this system, data were collected, processed and analyzed with DUAL PDA. The velocity profile and drop-size distribution (D10, D32, Dv0.9) downstream of the effervescent atomizer were gained. The efficiency of fire suppression of this system were experimentally investigated by putting out the fire of industry alcohol and diesel oil. The experimental results showed that this water mist system is feasible for fire suppression.
Comparison of image masking methods for pump impeller blade region PIV experiments
Xiaodong Liu,Tingting Yan,Qiang Zhong,Zhuqing Liu,Fujun Wang,Yaojun Li,Wei Yang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.6
Due to the blade reflection effect when applying the PIV technology to the internal flow field measurement of rotating machinery, the blade area requires special treatment. In this paper, zero-value, particle image, random number, and mean-value masking methods are compared and analyzed based on particle images from the PIV challenge and rotating impeller. The peak significance and coordinates deviations are used as the masking effect evaluation criteria. The results indicate that an increase in the interrogation window masking area proportion causes a weakening of the PIV calculation particle cluster displacement peak value. The peak position is shifted, which leads to the wrong velocity vector. The masking area proportion should not be higher than 0.7, 0.5, 0.19, and 0.22 for mean-value, random number, zero-value, and particle image masking methods, respectively. The results show that flow field calculation results near the blade boundary are more accurate for mean-value masking.
Bing Liu,Hai-Qiang Li,Hao-Bing Li,Jian Liu,Yi-Zhong Yang,Yan-Hui Lu,Abid Ali 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.2
Lygus pratensis distributes worldwide and is one of the common insect pests on cotton in Xinjiang Uygur Autonomous Region, the largest cotton growing region of China. This study assessed the effects of six constant temperatures (10, 15, 20, 25, 30 and 35 °C) at 60% relative humidity (RH) on egg and nymphal development of L. pratensis. Eggs failed to hatch and the newly-hatched nymphs could not normally develop at 10 °C. The developmental duration of egg and nymph decreased as temperature increased from 15 to 30 except 35 °C for egg. Based on the linear model, the lower developmental threshold and effective accumulated temperature were 10.68 °C and 150.2 DD for egg, and 12.08 °C and 208.3 DD for nymph, respectively. Among three non-linear models (Briere-1, Logan-6 and Lactin), Logan-6 provided the most accurate estimate for the mean optimum and lethal maximum temperatures (33.6 and 40.9 °C for egg, and 34.0 and 37.4 °C for nymphs). The interactive effects of three RH levels (45, 60 and 75%) and two temperatures (25 and 35 °C) on the immature developmental stages were tested. Temperature, RH and their interaction showed significant effects on egg and nymphal development. High relative humidity (75% RH) shortened the developmental duration of egg at 25 °C and nymph at 25 and 35 °C,whereas no significant difference was found for egg duration between different RH levels at 35 °C. The present study is useful for further predicting the phenology of L. pratensis and developing forecast and management strategies for this emerging mirid bug in China.