Highly Efficient Shoot Regeneration from Cotyledonary Nodes of Vegetable Soybean

Qian-Qian Liu,Gang Chen,Jun-Yi Gai,Yue-Lin Zhu,Li-Fei Yang,Guo-Ping Wei,Cong Wang 한국원예학회 2010 원예과학기술지 Vol.28 No.2

풋콩(Glycine max (L.) Merrill) 6개 품종의 자엽절 절편체로부터 thidiazuron(TDZ)과 NAA 농도를 달리한 배지에서 신초의 효율적인 재분화를 조사한 후, 가장 효과가 좋은 생장조절물질 조합으로 배지 종류, 접종방법 및 품종별 신초 재분화율을 조사하였다. 또한 절편체를 배지에 수직 또는 수평으로 치상하는 방법과 B5, 1/2 B5, MS, 1/2 MS, MSB(MS salts + B5 organics) 등의 5가지 기본 배지에 관하여 시험하였다. B5 배지에 1㎎?L<SUP>-1</SUP> TDZ, 0.05㎎?L<SUP>-1</SUP> NAA, 5㎎?L<SUP>-1</SUP> AgNO₃를 첨가한 처리에서 6개 품종의 신초 재분화율이 55.3-88.9%로 높았다. ‘L?ling No. 1’의 경우 수직으로 치상하는 것이 수평으로 치상하는 것보다 신초 재분화율이 37.5% 더 높았다. 따라서 풋콩은 TDZ를 처리한 B5 배지에 자엽절을 수직으로 치상할 때 신초 재분화율이 높았다. To establish a highly efficient system for shoot regeneration in vegetable soybean (Glycine max (L.) Merrill), explants were obtained from six genotypes and adventitious shoots were regenerated from cotyledonary nodes cultured on medium supplemented with different concentrations of N-phenyl-N′-1, 2, 3-thiadiazol-5-ylurea (thidiazuron TDZ) and 1-naphthylacetic acid (NAA). The most effective combination of plant growth regulators was selected first and then the effects of medium types, inoculation methods and genotypic differences on shoot regeneration were studied. The explants were inserted either vertically or horizontally into the medium and five basal media, including B5, ½ B5, MS, ½ MS, and MSB (MS salts and B5 organics), were tested. The shoot regeneration frequency of the six genotypes ranged from 53.5% to 88.9% and three of them reached 88.9%, 87.5% and 83.3%, respectively, on B5 medium supplemented with 1 ㎎?L<SUP>-1</SUP> TDZ, 0.05 ㎎?L<SUP>-1</SUP> NAA, and 5 ㎎?L<SUP>-1</SUP> AgNO₃. The shoot regeneration frequency of explants cultured on B5 medium was significantly higher than that of the other four basal media. The vertically inserted explants were found to yield a higher shoot regeneration frequency than that of horizontally inserted ones; the maximum difference of regenerating percentages between the two methods was 37.5%. In summary, TDZ was an efficient plant growth regulator for shoot induction. B5 medium and vertically inserted explants promoted shoot regeneration. We believe this highly efficient shoot regeneration system will provide foundation for the further transgenic studies in vegetable soybean.

High Efficient in Vitro Shoot Regeneration from Cotyledonary Nodes of Vegetable Soybean

Qian-Qian Liu,Jun-Yi Gai,Yue-Lin Zhu,Gang Chen,Li-Fei Yang,Guo-Ping Wei,Cong Wang 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-

Fault-tolerant Control for Linear System Under Sensor Saturation Constraint

Jun-Wei Zhu,Qiao-Qian Zhou,Jianming Xu,Jian-Wei Dong 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.1

An observer-based fault-tolerant control method is proposed for a linear system with sensor saturationconstraint. Considering the linear system with simultaneous actuator faults and sensor faults, the sensor saturation would bring the output measurement error of the system, which would result in the estimation performancedegradation. Firstly, the intermediate estimator is modified to estimate the system states and fault signals at thesimultaneous time, and the fault-tolerant controller is designed based on the estimation to compensate the effectof actuator faults effectively. Through Lyapunov stability analysis, the sufficient conditions are obtained to ensurethe states of closed-loop system to be uniformly ultimately bounded. The effect of sensor saturation error can besuppressed by adjusting some specified parameters directly without introducing any performance index. Finally, theeffectiveness and superiority of the proposed method are verified by a simulation example

Pin1 Promoter rs2233678 and rs2233679 Polymorphisms in Cancer: A Meta-analysis

Zhu, Yan-Mei,Liu, Jing-Wei,Xu, Qian,Yuan, Yuan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

PIN1 is one member of the parvulin PPIase family. By controlling Pro-directed phosphorylation, PIN1 plays an important role in cell transformation and oncogenesis. There are many polymorphisms in the PIN1 gene, including rs2233678 and rs2233679 affecting the PIN1 promoter. Recently, a number of case-control studies were conducted to investigate the association between PIN1 gene rs2233678 and rs2233679 polymorphism and cancer risk. However, published data are still conflicting. In this paper, we summarized data for 5,427 cancer cases and 5,469 controls from 9 studies and attempted to assess the susceptibility of PIN1 gene polymorphism to cancers by a synthetic meta-analysis. Odds ratios (ORs) with 95% confidence intervals (CIs) were estimated to assess the relationship. All analyses were performed using Stata software. Our results suggested that rs2233678 represented a protective factor in overall analysis (CC vs GG: OR= 0.697, 95%CI: 0.498-0.976; CG vs GG: OR=0.701, 95%CI: 0.572-0.858; Dominant model: OR= 0.707, 95%CI: 0.590-0.847; C allele vs G allele: OR=0.734, 95%CI: 0.623-0.867) and especially for squamous cell carcinoma of the head and neck, lung cancer and breast cancer in Asians and Caucasians. The rs2233679 polymorphism was significantly associated with decreased cancer risk in overall analysis (CT vs CC: OR=0.893, 95%CI=0.812-0.981; Dominant model: OR=0.893, 95%CI=0.816-0.976; T allele vs C allele; OR=0.947, 95%CI=0.896-1.000) and especially in Asians. In conclusion, our meta-analysis suggested that -842G>C (rs2233678) and -667C>T (rs2233679) may contribute to genetic susceptibility for cancer risks. Further prospective research with larger numbers of worldwide participants is warranted to draw comprehensive and firm conclusions.

Transcriptome analysis of colchicine-induced tetraploid Kiwifruit leaves with increased biomass and cell size

Zhu Yanyan,Tang Wei,Tang Xiaofeng,Wang Lihuan,Li Wei,Zhang Qian,Li Mingzhang,Fang Congbing,Liu Yongsheng,Wang Songhu 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.5

Colchicine-induced polyploidization has been extensively utilized in plant-breeding programs to increase biomass and overall yield of various crop species. Chromosome doubling usually increases the plant size and cell size. However, the underly- ing mechanisms remain elusive. In this study, we showed that 0.1% colchicine is an optimized concentration for inducing tetraploidization of Actinidia chinensis var. chinensis ‘Hongyang’, a commercially important diploid kiwifruit cultivar. The tetraploid plants showed increased plant height, leaf size, and biomass, as compared with the corresponding diploid plants. Scanning electron microscopy and histological analysis indicated that the leaf cell size was significantly increased in the tetraploid plants. Our further transcriptome analysis revealed the 5922 differentially expressed genes between the diploid and tetraploid plants. Gene Ontology analysis enriched the cell wall-related genes, including the pectin methylesterases (PMEs) and expansins (EXPs), both of which play a critical role in cell wall loosening and extension. The increased expression of PME and EXP genes might contribute to the increased cell size in the tetraploid plants. Together, our work indicated that tetraploidization increases the organ and cell size in kiwifruit, possibly by enhancing the cell wall extensibility.

Local Joule heating targets catalyst surface for hydrocarbon combustion

Qian Xiong,Xingbao Zhu,Ri He,Xueyi Mei,Yexin Zhang,Zhicheng Zhong,Wei Zhao,Weiming Nie,Jian Zhang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-

Most industrial catalytic reactions are achieved by external heating and catalysts are entirely heated tooffer enough thermal energy to surface active sites. However, there is an inherent drawback that mostinput energy is dissipated into the bulk while minor is donated to the surface, leading to high energywaste. Here, we proposed a so-called local Joule heating method via passing an electric current throughpacked catalyst nanoparticles with a large contact resistance, which can generate sufficient heat to targetat the surface region. We selected hydrocarbon combustion, a common way to eliminate unburned pollutants,as a probe reaction and used the conductive antimony-doped tin oxide (ATO) as a model catalyst. Compared with traditional external heating, this method consumed one order lower energy input,reduced the macroscopically average temperature for same conversion by 100 C, improved the durabilitywith smaller activity loss within 100 h operation, and suppressed water poisoning effect by 60 %. Also, the combustion was sparked in seconds by pulsing electric current into the catalyst bed, allowing anapplication in prompt treatment of leaked hydrocarbons. The local Joule heating between contactednanoparticles, which could focus thermal energy on catalyst surface, is prospective to improve catalysisefficiency.

New dammarane-type triterpenoid saponins from Panax notoginseng saponins

Qian Li,Mingrui Yuan,Xiaohui Li,Jinyu Li,Ming Xu,Di Wei,Desong Wu,Jinfu Wan,Shuangxi Mei,Tao Cui,Jingkun Wang,Zhaoyun Zhu 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.5

Background: Panax notoginseng saponin (PNS) is the extraction from the roots and rhizomes of Panax notoginseng (Burk.) F. H. Chen. PNS is the main bioactive component of Xuesaitong, Xueshuantong, and other Chinese patent medicines, which are all bestselling prescriptions in China to treat cardiocerebrovascular diseases. Notoginsenoside R₁ and ginsenoside Rg₁, Rd, Re, and Rb₁ are the principal effective constituents of PNS, but a systematic research on the rare saponin compositions has not been conducted. Objective: The objective of this study was to conduct a systematic chemical study on PNS and establish the HPLC fingerprint of PNS to provide scientific evidence in quality control. In addition, the cytotoxicity of the new compounds was tested. Methods: Pure saponins from PNS were isolated by means of many chromatographic methods, and their structures were determined by extensive analyses of NMR and HR-ESI-MS studies. The fingerprint was established by HPLC-UV method. The cytotoxicity of the compounds was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5 -diphenyltetrazolium bromide assay. Results and Conclusion: Three new triterpenoid saponins (1e3) together with 25 known rare saponins (4 e28) were isolated from PNS, except for the five main compounds (notoginsenoside R₁ and ginsenoside Rg₁, Rd, Re, and Rb₁). In addition, the HPLC fingerprint of PNS was established, and the peaks of the isolated compounds were marked. The study of chemical constituents and fingerprint was useful for the quality control of PNS. The study on antitumor activities showed that new Compound 2 exhibited significant inhibitory activity against the tested cell lines.

Phase Distribution and Coercivity Enhancement in (MM,Nd)-Fe-B Magnets with Dual-main-phase

Dong-wei Duan,Lei Wei,Zhu-bai Li,Qian Zhao,Yong-feng Li 한국자기학회 2021 Journal of Magnetics Vol.26 No.2

Identification of a C-type lectin from Antheraea pernyi, which may associate with the immune response to pathogens

Cen Qian,Kunzhang Lin,Baojian Zhu,Chao-Liang Liu,Lei Wang,Ya Liu,Dan Liang,PeiWang,Guo-Qing Wei 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

C-Type lectins (CTLs) are carbohydrate-recognition proteins and play critical roles in innate immunity. In this study, we characterized a CTL fromAntheraea pernyi, named ApCTL,which has two carbohydrate-recognition domains (CRDs). A fragment of 1017 bp cDNA of ApCTL was isolated, which constituted 927 bp open reading frame (ORF), and it encoded putative protein of 308 amino acids. The recombinant protein of ApCTL was expressed in Escherichia coli, and anti-ApCTL antibodieswere prepared. ApCTL genewas constitutively expressed in a variety of tissueswhile it showed a higher expression level in fat body than other tissues. Protein andmRNAlevels of ApCTL in fat body and hemolymph were elevated upon nuclear polyhedrosis virus (NPV) and E. coli challenges. Recombinant ApCTL increased the agglutination of E. coli in a Ca2+-dependent manner and had the bacteriolytic activity on E. coli. Our results suggested that ApCTLmay play critical roles in pathogen recognition and could be involved in the innate immunity of A. pernyi.

Identification and characterization of an Apolipophorin-III gene from Actias selene Hübner (Lepidoptera: Saturniidae)

Cen Qian, LeiWang,FangWang,Bao-Jian Zhu,Guo-Qing Wei,Sheng Li,Chao-Liang Liu,Lei Wang 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.1

Apolipophorin-III (ApoLp-III) is involved in lipid transport and innate immunity in insects. In this study, we isolated ApoLp-III gene (As-ApoLp-III) cDNA fromActias selene Hübner (Lepidoptera: Saturniidae). It contained 660 nucleotides with a putative open reading frame (ORF) of 561 bp encoding 186 amino acid residues. It showed similarities with ApoLp-III proteins from other insect species, especially Antheraea pernyi. The recombinant protein of As-ApoLp-III was expressed in Escherichia coli, and anti-As-ApoLp-III antibodies were prepared. The As-ApoLp-III gene was expressed at high levels in the fat body and integument, and at low levels in the hemolymph, testis, silk gland, malpighian tubule, ovary, and midgut. As-ApoLp-III gene can be upregulated by E. coli, Micrococcus luteus, Beauveria bassiana, and nuclear polyhedrosis virus (NPV) and showed a greater sensitivity for gram-negative bacteria and fungi than for gram-positive bacteria and virus. These data indicated that As-ApoLp-III may play an important role in innate immune responses of Actias selene.