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Ag2O nanoparticle clusters coated with porous gelatin-g-PMMA copolymer
Ying Liu,Xiaoheng Liu,Xiaodong Wu,Xujie Yang,Lude Lu 한국물리학회 2010 Current Applied Physics Vol.10 No.3
Herein a special nanoparticle cluster coated with a porous copolymer is designed and prepared. At first,Ag2O nanoparticles (secondary particles) were fabricated in gelatin solution by a facile chemical approach. Then these nanoparticles were entrapped in a copolymerization system containing gelatin,methyl methacrylate (MMA), an initiator, and using water as a solvent. The nanoparticle clusters coated with porous gelatin-g-PMMA copolymer (Ag2O/gelatin-g-PMMA) were prepared by grafting methyl methacrylate onto gelatin, followed by coating solidification. One significant feature for our approach is that every Ag2O aggregated cluster has been coated with porous gelatin-g-PMMA copolymer film in a unique way, and the Ag2O nanoparticle could penetrate and escape from the coating freely in water by ultrasonication. As a result, this study provides a new approach to prepare monodispersed nanoparticles by ordered porous copolymers with controlled releasing.
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Hybrid Differential Evolution Technique
Liu Xiao-Wen,Xujie Tan,Hyun-Chang Lee,Oh-Hyung Kang,Seong-Yoon Shin 한국정보통신학회 2021 2016 INTERNATIONAL CONFERENCE Vol.12 No.1
In this paper, we proposed a hybrid DE based on an ecological model algorithm called SparkHDE-EM, where we introduced a Spark-based island model to implement parallelization of different DE transformations and maintain a balance between resources using the Monod model. It includes three DE variants: jDE, JADE and CUDE.
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( Mulan Wei ),( Xujie Liu ),( Chunyu Cao ),( Jianlin Yang ),( Yafeng Lv ),( Jiaojiao Huang ),( Yanlin Wang ),( Ye Qin ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.11
Recent studies showed that the PD-1/PD-L1 checkpoint blockade is a dramatic therapy for melanoma by enhancing antitumor immune activity. Currently, major strategies for the PD-1/PD-L1 blockade have mainly focused on the use of antibodies and compounds. Seeking an alternative approach, others employ endogenous proteins as blocking agents. The extracellular domain of PD-1 (ePD1) includes the binding site with PD-L1. Accordingly, we constructed a PD-1-based recombinantly tailored fusion protein (dFv-ePD1) that consists of bivalent variable fragments (dFv) of an MMP-2/9-targeted antibody and ePD1. The melanoma-binding intensity and antitumor activity were also investigated. We found the intense and selective binding capability of the protein dFv-ePD1 to human melanoma specimens was confirmed by a tissue microarray. In addition, dFv-ePD1 significantly suppressed the migration and invasion of mouse melanoma B16-F1 cells, and displayed cytotoxicity to cancer cells in vitro. Notably, dFv-ePD1 significantly inhibited the growth of mouse melanoma B16-F1 tumor cells in mice and in vivo fluorescence imaging showed that dFv-ePD was gradually accumulated into the B16-F1 tumor. Also the B16-F1 tumor fluorescence intensity at the tumor site was stronger than that of dFv. This study indicates that the recombinant protein dFv-ePD1 has an intensive melanoma-binding capability and exerts potent therapeutic efficacy against melanoma. The novel format of the PD-L1-blocked agent may play an active role in antitumor immunotherapy. [BMB Reports 2018; 51(11): 572-577]
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Lu Wang,Xiaoheng Liu,Xin Wang,Xujie Yang,Lude Lu 한국물리학회 2010 Current Applied Physics Vol.10 No.6
Mesoporous Co3O4 microspheres with unique crater-like morphology were obtained by utilizing the mesoporous silica material MCM-41 as a template. The analysis results of N2 adsorptionedesorption measurement indicate that the product has a large BrunauereEmmetteTeller (BET) surface area of 60 m2 g-1 and a narrow pore size distribution centering around 3.7 nm. Its electrochemical properties were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)measurements. The findings reveal that this novel morphology material has a smaller inner resistance of about 0.4 U and a higher onset frequency of 550 Hz. This material can provide a high specific capacitance of 102 F g-1 and a large capacity retention of 74% in 500 continuous cycles test at a sweep rate of 3 mV s-1. More significantly, the mass loading of electroactive species can reach as large as 2 mg cm-2, which is one order of magnitude larger than common amount used.
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Self-bending extrusion molding of distorted channels
Fanlei Min,Huiping Liu,Guangming Zhu,Zheng Chang,Xujie Gao,Bowen Yue,Nana Guo,Xiaoqing Zhai 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.5
Using an integrated profile extrusion and bending forming process with a streamlined extrusion die, a new self-bending extrusion molding technology is proposed with an axis-distorted variable channel. By designing the streamlined extrusion die structure of the distorted central axis, the metal was made to flow non-uniformly in the die cavity, thereby directly extruding a bent profile. The central axis of the streamlined extrusion die is described by a trigonometric function and a Gaussian function. A numerical simulation was applied to analyze the metal flow pattern, equivalent strain, and strain-rate distribution during the self-bending extrusion process. The influences of the extrusion velocity and the addition of a bearing on the self-bending deformation profiles were investigated. During the extrusion process, the streamline at the center of the billet could describe the overall flow of the metal in the die cavity, and the distance between the point on the end face of the die outlet and the center of the die outlet directly determined the degree of extrusion and bending. The greater the distance was, the larger was the degree of bending. The metal strain on the convex edge of the die was greater than that on the concave edge of the die, with the extruded profile always bending toward the concave edge. The strain rate of the metal changed the fastest near the most convex point of the die. As the extrusion velocity increased or more bearings were added, the radius of curvature of the extruded profile increased nonlinearly.
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Ling Zhao,Liang Zhang,Xiaona Shi,Xujie Duan,Huiping Li,Shuying Liu 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.6
Background: Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide. Objectives: In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated. Methods: Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis. Results: A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%–86.8% and 67.7%–75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5). Conclusions: The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.
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