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Qin Ye,Yonggang Wang,Peng Du,Renbao Gan,Zhimin Li 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2
Secretion of the expressed heterologous proteins can reduce the stress to the host cells and is beneficial to their recovery and purification. In this study, fed-batch cultures of Escherichia coli YK537 (pAET-8) were conducted in a 5-L fermentor for the secretory production of human epidermal growth factor (hEGF) whose expression was under the control of alkaline phosphatase promoter. The effects of feeding of glucose and complex nitrogen sources on hEGF production were investigated. When the fed-batch culture was conducted in a chemically defined medium, the cell density was 9.68 g/L and the secreted hEGF was 44.7 mg/L in a period of 60 h. When a complex medium was used and glucose was added in pH-stat mode, the secreted hEGF was improved to 345 mg/L. When the culture was fed with glucose at a constant specific rate of 0.25 gg-1h-1, hEGF reached 514 mg/L. The effects of adding a solution containing yeast extract and tryptone were further studied. Different rate of the nitrogen source feeding resulted in different levels of phosphate and acetic acid formation, thus affected hEGF expression. At the optimal feeding rate, hEGF production achieved 686 mg/L.
Renan Qin,Hong Nie,Jianyu Zhang,Chuyuan Li,Xiaoqi Zhang,Aihua Xiong,Feng Huang,Zhen Yin,Kongyan Li,Wenyu Qin,Mingzhen Chen,Shubing Zhang,Lingyi Liang,Huiye Zhang,Wencai Ye 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.7
In the present study, the protective effects of gypenosides from Gynostemma pentaphyllum on fatty liver disease (FLD) were examined in rats treated with high fat and cholesterol diet and alcohol. Male SD rats were divided into seven groups: control, model, lovastatin, silymarin, gypenosides high-, medium- and low-treatment groups. The latter 6 groups were fed high-fat and cholesterol diet and administered alcohol intragastricly once a day. Body weight was measured every week for 10 weeks, and the hepatic index was measured after 10 weeks. Compared with model group, levels of serum triglyceride (TG), total cholesterol (TC), free fatty acid (FFA), and low density lipoprotein cholesterol (LDL-C) level, malondialdehyde (MDA), serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity, and hepatocyte apoptosis were significantly decreased in gypenosides groups; while serum high density lipoprotein cholesterol (HDL-C), superoxide dismutase (SOD) activity in both serum and hepatic tissue and mRNA and protein level of peroxisome proliferator-activated receptor α (PPAR-α) were significantly increased. Moreover, hepatic steatosis and mitochondrial damage were improved. These results suggested that gypenosides could prevent liver fatty degeneration in fatty liver disease through modulating lipid metabolism, ameliorating liver dysfunction and reducing oxidative stress.
Ye, Yan-Qing,Xia, Cong-Fang,Yang, Juan-Xia,Yang, Yu-Chun,Qin, Ying,Gao, Xue-Mei,Du, Gang,Li, Xue-Mei,Hu, Qiu-Fen Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.10
Two new butyrolactones, asperphenol A (1) and B (2), together with four known butyrolactones (3-6) were isolated from the fermentation products of an endophytic fungus Aspergillus versicolor. Their structures were elucidated by spectroscopic methods including extensive 1D- and 2D-NMR techniques. Compounds 1-6 were also tested for their anti-tobacco mosaic virus (anti-TMV) activities. The results showed that compound 2 exhibited high anti-TMV activity with inhibition rate of 46.7%. The other compounds also exhibited potential anti-TMV activities with inhibition rates in the range of 21.8-28.4%.
Qin, Ai-Qiu,Liang, Zhong-Guo,Ye, Jia-Xiang,Li, Jing,Wang, Jian-Li,Chen, Chang-Xian,Song, Hong-Lin Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.8
Background: Whether concurrent chemotherapy treatment is superior to radiotherapy alone as an adjuvant regimen for postoperative cervical carcinoma with risk factors remains controversial. Materials and Methods: A literature search strategy examined Pubmed, Embase, the Cochrane Library, the China National Knowledge Internet Web, the Chinese Biomedical Database and the Wanfang Database. Article reference lists and scientific meeting abstracts were also screened. Controlled trials comparing concurrent chemoradiotherapy versus radiotherapy alone in postoperative cervical cancer were included. The methodological quality of non-randomized controlled trials was evaluated using the Newcastle-Ottawa Scale. Randomized controlled studies were evaluated with the Cochrane handbook. A meta-analysis was performed with RevMan 5.3. Results: A total of 1,073 patients from 11 clinical trials were analysed, with 582 patients in the concurrent chemoradiotherapy group and 491 patients in the radiotherapy group. Hazard ratios (HR) of 0.47 (95% CI 0.31-0.72) and 0.50 (95% CI 0.35-0.72) were observed for overall survival and progression-free survival, indicating a benefit from the additional use of concurrent chemotherapy. Subgroup analyses demonstrated that cervical cancer with high risk factors significantly benefitted from concurrent chemotherapy when examining overall survival (HR 0.44, 95% CI 0.28-0.67) and progression-free survival (HR 0.48, 95% CI 0.33-0.70), but patients with intermediate risk factors showed no benefit from concurrent chemotherapy in overall survival (HR 1.72, 95% CI 0.28-10.41) and progression-free survival (HR 1.09, 95% CI 0.19-6.14). No significant differences were observed for grade 3-4 anaemia (risk ratio (RR) 3.87, 95% CI 0.69-21.84), grade 3-4 thrombocytopenia (RR 3.04, 95% CI 0.88-10.58), grade 3-4 vomiting or nausea (RR 1.71, 95% CI 0.27-10.96), or grade 3-4 diarrhoea (RR 1.40, 95% CI 0.69-2.83). Significant differences were observed for grade 3-4 neutropenia in favour of the radiotherapy group (RR 7.23, 95% CI 3.94-13.26). Conclusions: In conclusion, concurrent chemoradiotherapy improves survival in postoperative cervical cancer with high risk factors but not in those with intermediate risk factors.
Study on the Flavor Contribution of Phospholipids and Triglycerides to Pork
Ye-Chuan Huang,Hong-Jun Li,Zhi-Fei He,Ting Wang,Gang Qin 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.5
For investigating the flavor contribution of phospholipids and triglycerides to pork, the longissimus muscle of Rongchang (RC) and PIC (bred by PIC Company of England) pig were selectively removed of intramuscular triglycerides or total intramuscular lipids. After cooking, the flavor of different samples was compared by sensory evaluation, gas chromatographymass spectrometry (GC-MS), and electronic nose. The results showed that removing triglycerides had little effect on the aroma of the cooked meat, but when total intramuscular lipids were removed a remarkable difference was observed. The control samples covaried strongly with some lipid-derived products, and the petroleum ether defatted samples also positively related to these compounds,but to a lesser extent, both of them negatively covaried with chloroform-methanol extracted samples which associated with some Maillard reaction products. Moreover the flavor difference between 2 breeds would decrease when triglycerides were removed, and reduce further when phospholipids and triglycerides were removed simultaneously. These results suggested that intramuscular lipids especially phospholipids contributed significantly to pork flavor, the flavor difference between 2 breeds resulted mainly from different intramuscular lipids content and composition.
Mao, Ye-Qing,Xu, Xin,Lin, Yi-Wei,Chen, Hong,Hu, Zheng-Hui,Xu, Xiang-Lai,Zhu, Yi,Wu, Jian,Zheng, Xiang-Yi,Qin, Jie,Xie, Li-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12
Insulin-like growth factor-binding protein-3 (IGFBP3) has been identified as a putative tumor suppressor with multifunctional roles in the IGF axis. Recently, there have been a growing body of studies investigating the relation between the IGFBP3 A-202C polymorphism, circulating IGFBP3 and prostate cancer risk, but their outcomes varied leading to controversy. Hence, it is necessary to perform a meta-analysis covering all eligible studies to shed a light on the association of IGFBP3 A-202C and cancer risk. Finally, we included a total of 11 relevant articles between 2003 and 2010 covering 14 case-control studies including 9,238 cases and 8,741 controls for our analysis. Our results showed that A-202C was a marginal risk factor of prostate cancer (allele contrast: OR=1.08, 95% CI :1.01-1.16; dominant model: OR=1.11, 95% CI :1.01-1.22; heterozygote codominant model: OR=1.11, 95% CI :1.03-1.18; homozygote contrast: OR=1.19, 95% CI :1.03-1.37). Stratification analysis revealed that sample size and control source were two major heterogeneous meta-factors especially in the recessive model (source: Population-based control group :p=0.30,I2=16.7%, Hospital-based control group: p=0.20, I2=30.3%; sample size: Small: p=0.22,I2= 32.8%, Medium: p=0.09,I2=48%, Large p=0.60,I2=0.0%); However, contrary to previous findings, no significance was found in racial subgroups. No significant publication bias was found in our analysis. Considering the robustness of the results and the discrepancy among some studies, there might be some unsolved confounding factors, and further more critical large studies are needed for confirmation.
Inkjet Printed Quantum Dots Color Conversion Layers for Full-color Micro-LED Displays
Feng Qin,Cao Liu,Wenhui Wu,Wenxiang Peng,Sitao Huo,Jiandong Ye,Shulin Gu 대한금속·재료학회 2023 ELECTRONIC MATERIALS LETTERS Vol.19 No.1
With the ever-growing demands for larger size and high resolution displays, Micro-light-emitting diode (Micro-LED) displaywith quantum dots (QDs) fi lm as color conversion layers (CCLs) has become one of the most promising candidates offuture display for its advantages in low power consumption and wide color range. In this study, we report a novel full-colordisplay based on blue Micro LED, which has patterned red and green QDs color conversion (QDCC) layers fabricated byinkjet printing (IJP). A structure of double-layer bank was designed to reduce color deviation, prevent crosstalk, and fl attenthe QDCC layer. By optimizing the thickness of the red/green QDCC layers and the wavelength of blue Micro LEDbacklights, a full-color QDCC-LED display with 228 PPI resolution and size of 1.11-inch was successfully fabricated andshowed superb performance. We not only eff ectively reduced crosstalk, but also improved the color conversion effi ciency ofQDs. In addition, this QDCC-LED display prepared by embedded bonding process shows a color gamut of 107.53% NTSC.
Xu Ye,Dietrich Christopher H.,Qin Dao-Zheng 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1
Two genera, Dayus Mahmood, 1967 and Znana Dworakowska, 1994 of the leafhopper tribe Empoascini (Hem iptera: Cicadellidae: Typhlocybinae) are reviewed. One new species of each genus, Dayus furcatus sp. nov. and Znana furca sp. nov., are described from Thailand. A checklist and distribution summary of Dayus Mahmood species is given. Male habitus photos and illustrations of male genitalia of the two new species and notes on allied species are also provided.