Utilization Patterns of Disease-Modifying Antirheumatic Drugs in Elderly Rheumatoid Arthritis Patients

Xue-Mei Jin,이중엽,최남경,성종미,신주영,김예지,김미숙,양보람,박병주 대한의학회 2014 Journal of Korean medical science Vol.29 No.2

This study was conducted to investigate disease-modifying antirheumatic drug (DMARD) utilization in Korean elderly patients with rheumatoid arthritis (RA). We used data from January 1, 2005 to June 30, 2006 from the Health Insurance Review and Assessment Service claims database. The study subjects were defined as patients aged 65 yr or older with at least two claims with a diagnosis of RA. DMARD use was compared by the patients’ age-group, gender, medical service, and geographic divisions. The patterns of DMARD use in mono- and combination therapy were calculated. RA medication use was calculated by the number of defined daily doses (DDD)/1,000 patients/day. A total of 166,388 patients were identified during the study period. DMARD use in RA patients was 12.0%. The proportion of DMARD use was higher in the younger elderly, females, and patients treated in big cities. Hydroxychloroquine was the most commonly used DMARD in monotherapy, and most of the combination therapies prescribed it with methotrexate. DMARD use in elderly RA patients was noticeably low, although drug prescriptions showed an increasing trend during the study period, clinicians may need to pay more attention to elderly RA patients.

<i>H2AFX</i> Polymorphisms Are Associated with Decreased Risk of Diffuse Large B Cell Lymphoma in Koreans

Jin, Xue Mei,Kim, Hee Nam,Shin, Min-Ho,Lee, Il-Kwon,Lee, Ji Shin,Lee, Jae-Hyuk,Kim, Hyeoung-Joon,Choi, Jin-Su,Juhng, Sang Woo,Choi, Chan Mary Ann Liebert 2011 DNA and cell biology Vol.30 No.12

<P>Polymorphisms of the H2A histone family member X (H2AFX) gene have been associated with decreased non-Hodgkin lymphoma (NHL, -417AA) risk and increased breast cancer (1654AG/GG, and -1420GA/AA) risk. We investigated whether H2AFX polymorphisms are associated with the risk of NHL and its subtypes in 573 NHL Korean patients and 721 cancer-free control subjects, using high resolution melting polymerase chain reaction and an automatic sequencer. There was no association between polymorphisms and the risk of overall NHL, all B cell lymphoma, or all T cell lymphoma. However, the -1420 AA genotype was associated with decreased diffuse large B cell lymphoma (DLBCL) risk (OR, 0.65; 95% CI, 0.43-0.97), and there was a trend for allele dose-effect (p-trend=0.03). The -1187 CC genotype was associated with decreased DLBCL risk with borderline significance (OR, 0.70; 95% CI, 0.48-1.02). There was a trend for an allele dose-effect with borderline significance (p-trend=0.06). These results suggest that the -1420 AA genotype of H2AFX may be associated with reduced DLBCL risks in the Korean population.</P>

Effects of Increased NADPH Concentration by Metabolic Engineering of the Pentose Phosphate Pathway on Antibiotic Production and Sporulation in Streptomyces lividans TK24

( Xue-mei Jin ),( Yong-keun Chang ),( Jae Hag Lee ),( Soon-kwang Hong ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.10

Most of the biosynthetic pathways for secondary metabolites are influenced by carbon metabolism and supply of cytosolic NADPH. We engineered carbon distribution to the pentose phosphate pathway (PPP) and redesigned the host to produce high levels of NADPH and primary intermediates from the PPP. The main enzymes producing NADPH in the PPP, glucose 6-phosphate dehydrogenase (encoded by zwf1 and zwf2) and 6-phosphogluconate dehydrogenase (encoded by zwf3), were overexpressed with opc encoding a positive allosteric effector essential for Zwf activity in various combinations in Streptomyces lividans TK24. Most S. lividans transformants showed better cell growth and higher concentration of cytosolic NADPH than those of the control, and S. lividans TK24/pWHM3-Z23O2 containing zwf2+zwf3+opc2 showed the highest NADPH concentration but poor sporulation in R2YE medium. S. lividans TK24/pWHM3-Z23O2 in minimal medium showed the maximum growth (6.2 mg/ml) at day 4. Thereafter, a gradual decrease of biomass and a sharp increase of cytosolic NADPH and sedoheptulose 7-phosphate between days 2 and 4 and between days 1 and 3, respectively, were observed. Moreover, S. lividans TK24/pWHM3-Z23O2 produced 0.9 times less actinorhodin but 1.8 times more undecylprodigiosin than the control. These results suggested that the increased NADPH concentration and various intermediates from the PPP specifically triggered undecylprodigiosin biosynthesis that required many precursors and NADPH-dependent reduction reaction. This study is the first report on bespoke metabolic engineering of PPP routes especially suitable for producing secondary metabolites that need diverse primary precursors and NADPH, which is useful information for metabolic engineering in Streptomyces.

SCO6992, a Protein with β-Glucuronidase Activity, Complements a Mutation at the absR Locus and Promotes Antibiotic Biosynthesis in Streptomyces coelicolor

( Xue-mei Jin ),( Mu-yong Choi ),( Maral Tsevelkhoroloo ),( Uhnmee Park ),( Joo-won Suh ),( Soon-kwang Hong ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.11

Streptomyces coelicolor is a filamentous soil bacterium producing several kinds of antibiotics. S. coelicolor abs8752 is an abs (antibiotic synthesis deficient)-type mutation at the absR locus; it is characterized by an incapacity to produce any of the four antibiotics synthesized by its parental strain J1501. A chromosomal DNA fragment from S. coelicolor J1501, capable of complementing the abs- phenotype of the abs8752 mutant, was cloned and analyzed. DNA sequencing revealed that two complete ORFs (SCO6992 and SCO6993) were present in opposite directions in the clone. Introduction of SCO6992 in the mutant strain resulted in a remarkable increase in the production of two pigmented antibiotics, actinorhodin and undecylprodigiosin, in S. coelicolor J1501 and abs8752. However, introduction of SCO6993 did not show any significant difference compared to the control, suggesting that SCO6992 is primarily involved in stimulating the biosynthesis of antibiotics in S. coelicolor. In silico analysis of SCO6992 (359 aa, 39.5 kDa) revealed that sequences homologous to SCO6992 were all annotated as hypothetical proteins. Although a metalloprotease domain with a conserved metal-binding motif was found in SCO6992, the recombinant rSCO6992 did not show any protease activity. Instead, it showed very strong β-glucuronidase activity in an API ZYM assay and toward two artificial substrates, p-nitrophenyl-β-D-glucuronide and AS-BI-β-D-glucuronide. The binding between rSCO6992 and Zn²⁺ was confirmed by circular dichroism spectroscopy. We report for the first time that SCO6992 is a novel protein with β-glucuronidase activity, that has a distinct primary structure and physiological role from those of previously reported β-glucuronidases.

서파 오도일 「丙寅燕行日乘」의 내용상의 특징

김설매(JIN XUE MEI) 한국언어문학회 2015 한국언어문학 Vol.93 No.-

Oh Doil(1645-1703)was a scholar and politician of the Joseon dynasty in 17th century. In June 1868, he started off to Peking as Seojanggwan(an official in charge of keeping records) and returned in November the same year. He wrote the ByeongInYeonHaengIISung during the journey. The main content of the ByeongInYeonHaengIISung focused on the journey from ApRokGang to Peking and the days he stayed in Peking. The returning course only took a small part in the book. Entries in ByeongInYeonHaengIISung were mostly written in essay which is more flexible and detailed compared to the poetries in early Yeonhaengroks. In ByeongInYeonHaengIISung, Oh Doil recorded information about the politics, economy, culture and many other aspects of Qing dynasty at the time which is very valuable for the study of the period in China. At the beginning of the Qing dynasty, most scholars in Joseon dynasty considered Qing as barbarian, and don't think it'll stay for long. In ByeongInYeonHaengIISung, although Oh Doil still predicted the Qing dynasty's destiny the same way, but with a little reluctant he started to recognized the economy development and civilization in Qing which is an example that the attitudes of Joseon scholars towards Qing gradually changed through time.

문장부호를 사용한 효과적인 중국어 최장명사구 식별기법

백설매(Xue-Mei Bai),이금희(Jin-Ji Li),김미훈(Mei-Xun Jin),정유진(You-Jin Cheng),이종혁(Jong-Hyeok Lee) 한국정보과학회 2005 한국정보과학회 학술발표논문집 Vol.32 No.1

일반적으로 중국어의 명사구는 최단명사구, 기본명사구, 최장명사구로 분류된다. 최장명사구에 대한 정확한 식별은 문장의 전체적인 구조를 파악하고 문장의 정확한 지배용언을 찾아내는데 중요한 역할을 한다. 본 논문에서는 특성에 따라 5개의 클래스로 세분화된 문장부호를 학습자질로 사용하여 최장명사구 자동식별을 진행한다. 제안된 기법은 평균길이가 4인 최장명사구의 식별실험에서 기본모델(baseline)보다 4.5% 향상된 평균 85.1%의 우수한 F-measure 성능을 보인다.

문장부호 정보와 확장된 청크에 기반한 중국어 최장명사구 식별

백설매(Xue-Mei Bai),김미훈(Mei-Xun Jin),이금희(Jin-Ji Li),정유진(You-Jin Chung),이종혁(Jong-Hyeok Lee) 한국정보과학회 언어공학연구회 2005 한국정보과학회 언어공학연구회 학술발표 논문집 Vol.2005 No.10

명사구는 기본명사구와 최장명사구로 분류된다. 최장명사구에 대한 정확한 식별은 문장의 전체적인 구문구조를 파악하고 문장의 정확한 지배용언을 찾아내는데 중요한 역할을 수행한다. 본 논문에서는 확장된 청크(chunk) 개념과 다섯 개의 클래스로 세분화된 문장부호 정보를 사용한 최장명사구 식별 기법을 제안한다. 제안된 기법은 기본모델(baseline)보다 4.05% 향상된 평균 88.63%의 우수한 F-measure 성능을 보인다.

L-carnitine treatment attenuates renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction

( Hai Yan Zhao ),( Hui Ying Li ),( Jian Jin ),( Ji Zhe Jin ),( Long Ye Zhang ),( Mei Ying Xuan ),( Xue Mei Jin ),( Yu Ji Jiang ),( Hai Lan Zheng ),( Ying Shun Jin ),( Yong Jie Jin ),( Bum Soon Choi ) 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.0

Background/Aims: Accumulating evidence indicates that L-carnitine (LC) protects against multiorgan damage through its antioxidant properties and preservation of the mitochondria. Little information is available about the effects of LC on renal fibrosis. This study examined whether LC treatment would provide renoprotection in a rat model of unilateral ureteral obstruction (UUO) and in vitro. Methods: Sprague-Dawley rats that underwent UUO were treated daily with LC for 7 or 14 days. The influence of LC on renal injury caused by UUO was evaluated by histopathology, and analysis of gene expression, oxidative stress, mitochondrial function, programmed cell death, and phosphatidylinositol 3-kinase (PI3K)/ AKT/forkhead box protein O 1a (FoxO1a) signaling. In addition, H₂O₂-exposed human kidney cells (HK-2) were treated with LC. Results: LC treatment inhibited expression of proinflammatory and profibrotic cytokines, and was followed by a significant attenuation of tubulointerstitial inflammation and fibrosis. The increased oxidative stress caused by UUO was associated with mitochondrial dysfunction and excessive apoptosis and autophagy via PI3K/AKT/FoxO1a-dependent signaling, and this was abrogated by administration of LC. In H₂O₂-exposed HK-2 cells, LC decreased intracellular production of reactive oxygen species, and suppressed expression of profibrotic cytokines and reduced the number of apoptotic cells. Conclusions: LC protects against the progression of tubulointerstitial fibrosis in an obstructed kidney.

Curcumin Induces Apoptosis in SGC-7901 Gastric Adenocarcinoma Cells via Regulation of Mitochondrial Signaling Pathways

Xue, Xia,Yu, Jin-Long,Sun, De-Qing,Kong, Feng,Qu, Xian-Jun,Zou, Wen,Wu, Jing,Wang, Rong-Mei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

Curcumin, a polyphenol compound derived from the rhizome of the plant Curcuma longa L. has been verified as an anticancer compound against several types of cancer. However, understanding of the molecular mechanisms by which it induces apoptosis is limited. In this study, the anticancer efficacy of curcumin was investigated in human gastric adenocarcinoma SGC-7901 cells. The results demonstrated that curcumin induced morphological changes and decreased cell viability. Apoptosis triggered by curcumin was visualized using Annexin V-FITC/7-AAD staining. Curcumin-induced apoptosis of SGC-7901 cells was associated with the dissipation of mitochondrial membrane potential (MMP) and the release of cytochrome c into the cytosol. Furthermore, the down-regulation of Bcl-2 and up-regulation of Bax that led to the cleavage of caspase-3 and increased cleaved PARP was observed in SGC-7901 cells treated with curcumin. Therefore, curcumin-induced apoptosis of SGC-7901 cells might be mediated through the mitochondria pathway, which gives the rationale for in vivo studies on the utilization of curcumin as a potential cancer therapeutic compound.

Azidothymidine Downregulates Insulin-Like Growth Factor-1 Induced Lipogenesis by Suppressing Mitochondrial Biogenesis and Mitophagy in Immortalized Human Sebocytes

( Jin Gwi Yoo ),( Xue Mei Li ),( Jae Kyung Lee ),( Sanghyun Park ),( Dongkyun Hong ),( Kyung Eun Jung ),( Young Lee ),( Young-joon Seo ),( Chang Deok Kim ),( Jung-min Shin ),( Chong Won Choi ) 대한피부과학회 2021 Annals of Dermatology Vol.33 No.5

Background: Increased sebum secretion is considered the main causative factor in the pathogenesis of acne. There is an unmet pharmacological need for a novel drug that can control sebum production with a favorable adverse effect profile. Objective: To investigate the effect of azidothymidine on lipid synthesis in sebocytes and to identify the underlying mechanism of the inhibitory effect of azidothymidine on insulin-like growth factor (IGF)-1-induced lipid synthesis in sebocytes. Methods: Immortalized human sebocytes were used for the analysis. Thin-layer chromatography (TLC) and Oil Red O staining were performed to evaluate lipid synthesis in the sebocytes. The differentiation, lipid synthesis, mitochondrial biogenesis, and mitophagy in sebocytes were investigated. Results: TLC and Oil Red O staining revealed that azidothymidine reduced IGF-1 induced lipid synthesis in the immortalized human sebocytes. Azidothymidine also reduced IGF-1-induced expression of transcriptional factors and enzymes involved in sebocyte differentiation and lipid synthesis, respectively. Moreover, we found that IGF-1 upregulated the levels of peroxisome proliferator-activated receptor-gamma coactivator-1α, LC-3B, p62, and Parkin, major regulators of mitochondrial biogenesis and mitophagy in immortalized human sebocytes. In contrast, azidothymidine inhibited IGF-1 induced mitochondrial biogenesis and mitophagy in the sebocytes. Conclusion: These results suggest that azidothymidine downregulates IGF-1-induced lipogenesis by dysregulating the quality of mitochondria through suppression of mitochondrial biogenesis and mitophagy in immortalized human sebocytes. Our study provides early evidence that azidothymidine may be an effective candidate for a new pharmacological agent for controlling lipogenesis in sebocytes. (Ann Dermatol 33(5) 425∼431, 2021)