大黑山島 隣近 島嶼의 有用 植物遺傳資源 蒐集

Wan Sik Ahn(安完植),Jong Woong Ahn(安鍾雄) 한국육종학회 1992 한국육종학회지 Vol.24 No.2

The exploring mission to collect wild soybean and useful plant genetic resources was carried out at the Huksan archipelago of the southwestern part of Korean peninsula during October 28 to November 3, 1990. Totally 31 plants classified into 14 families, 18 genera and 22 species were collected. Among them, 9 collected leguminosae plants including one cultivated kidney bean belonged to 4 genera and 5 species. Useful leguminosae plants collected consisted of Rhynchosia volubilis Lour, R. acuminatifolia Makino, Amphicarpaea edgeworthii var. trisperma Ohwi and Dunbaria villosa (Thunb.) Makino and 8 collected samples belonged to 3 genera and 4 species. R. volubilis Lour at Hongdo island and R. acuminatifolia Makino and D. villosa at Bigeumdo could be collected, repectively, while A. edgeworthii var. trisperma could be observed at any exploring places. It was thought that these results were due to the limitation of place and time collected. Japanese black pine, lentinus chestnut, Camellia japonica, and Machilus thunbergii had been naturally grown from place to place at the part of Huksan county. On the contrary, it has been thought that rare plants as well as wild oriental orchid, locally called “Pungnan” might be endangered and so could be hardly found anywhere to collect. More efforts and supports to conserve nature and resources at a sustainable level should be requested than ever.

Correlations of 3T DCE-MRI Quantitative Parameters with Microvessel Density in a Human-Colorectal-Cancer Xenograft Mouse Model

Ahn, Sung Jun,An, Chan Sik,Koom, Woong Sub,Song, Ho-Taek,Suh, Jin-Suck The Korean Society of Radiology 2011 KOREAN JOURNAL OF RADIOLOGY Vol.12 No.6

<P><B>Objective</B></P><P>To investigate the correlation between quantitative dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) parameters and microvascular density (MVD) in a human-colon-cancer xenograft mouse model using 3 Tesla MRI.</P><P><B>Materials and Methods</B></P><P>A human-colon-cancer xenograft model was produced by subcutaneously inoculating 1 × 10<SUP>6</SUP> DLD-1 human-colon-cancer cells into the right hind limbs of 10 mice. The tumors were allowed to grow for two weeks and then assessed using MRI. DCE-MRI was performed by tail vein injection of 0.3 mmol/kg of gadolinium. A region of interest (ROI) was drawn at the midpoints along the z-axes of the tumors, and a Tofts model analysis was performed. The quantitative parameters (K<SUP>trans</SUP>, K<SUB>ep</SUB> and V<SUB>e</SUB>) from the whole transverse ROI and the hotspot ROI of the tumor were calculated. Immunohistochemical microvessel staining was performed and analyzed according to Weidner's criteria at the corresponding MRI sections. Additional Hematoxylin and Eosin staining was performed to evaluate tumor necrosis. The Mann-Whitney test and Spearman's rho correlation analysis were performed to prove the existence of a correlation between the quantitative parameters, necrosis, and MVD.</P><P><B>Results</B></P><P>Whole transverse ROI of the tumor showed no significant relationship between the MVD values and quantitative DCE-MRI parameters. In the hotspot ROI, there was a difference in MVD between low and high group of K<SUP>trans</SUP> and K<SUB>ep</SUB> that had marginally statistical significance (<I>ps</I> = 0.06 and 0.07, respectively). Also, K<SUP>trans</SUP> and K<SUB>ep</SUB> were found to have an inverse relationship with MVD (<I>r</I> = -0.61, <I>p</I> = 0.06 in K<SUP>trans</SUP>; <I>r</I> = -0.60, <I>p</I> = 0.07 in K<SUB>ep</SUB>).</P><P><B>Conclusion</B></P><P>Quantitative analysis of T1-weighted DCE-MRI using hotspot ROI may provide a better histologic match than whole transverse section ROI. Within the hotspots, K<SUP>trans</SUP> and K<SUB>ep</SUB> tend to have a reverse correlation with MVD in this colon cancer mouse model.</P>

Decellularized Human Adipose Tissue as an Alternative Graft Material for Bone Regeneration

Ahn Woo Beom,Lee Yu Bin,Ji Yi-Hwa,Moon Kyoung-Sik,Jang Hyon-Seok,Kang Sun-Woong 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.5

BACKGROUND: Tissue engineering approaches to treat damaged bone include various tissue transplants such as autologous, allogeneic, and xenografts. Artificial materials have been widely introduced to meet the demand for graft materials, but insufficiency in supply is still not resolved. In this study, human adipose tissue, easily obtained from the human body, was harvested, and the tissue was decellularized to fabricate a decellularized human adipose tissue matrix (DM) as an alternative graft material. METHODS: Human adipose tissue was obtained via liposuction. The obtained fresh adipose tissue sample was cut into pieces then put into decellularization solution (1% antibiotic–antimycotic solution and 1% phenylmethanesulphonyl fluoride). Lipids were further removed via treatment in isopropanol. The sample was then subjected to another enzymatic digestion and lipid removal processes. The obtained decellularized adipose tissue matrix was lyophilized to form a graft material in disc shape. RESULTS: Decellularization was confirmed by nuclear staining methods and detection of RNA and DNA via PCR. Bone morphogenetic protein 2 (BMP2)-loaded DM showed the ability to form new bone tissue when implanted in subcutaneous tissue. In recovery of a mouse calvarial defect model, BMP2-loaded DM exhibited similar levels of bone tissue regeneration efficiency compared with a well-defined commercial product, BMP2-loaded CollaCote. CONCLUSION: The DM developed in this study is expected to address the problem of insufficient supply of graft materials and contribute to the treatment of bone defects of critical size as an alternative bone graft material with preserved extracellular matrix components.

고혈압환자에서 혈압상승에 따른 심박동수의변이

안명수,김용훈,곽현진,안병진,윤광식,이홍순,유수웅 대한고혈압학회 1997 대한고혈압학회지 Vol.3 No.2

The Role of Radiation Therapy on Local Recurrence of Rectal Cancer

Woong Ki Chung,Sung Ja Ahn,Taek Keun Nam,Byung Sik Nah,Young Jin Kim 대한방사선종양학회 1992 Radiation Oncology Journal Vol.10 No.2

Ninety five patients of rectal cancer treated with surgery with or without adjuvant radiation therapy since January 1982 to December 1990 at the Chonnam University Hospital were analysed retrospectively regarding local failure. Of these 95 patients 72 patients were treated with surgery alone and remaining 23 patients received postoperative radiation therapy to pelvis. There were 45 men and 50 women with 53 years of median age. Minimum follow-up period was 19 months and median was 47 months (range, 19-125 months). Kaplan-Meier method was used to calculate actuarial risk of local recurrence and survival rate. Comparison between two groups was evaluated by Log rank test. Of total 95 patients twenty seven patients (28.4%) developed local recurrence and 13 patients (17.3%) developed local and distant metastasis concomitantly. Eighty nine percent (24/27) of patients developed local recurrence within 24 months. Pelvic organ adjacent to the primary tumor area was the most common site of initial local recurrence. Of 72 patients treated with surgery alone local recurrence developed in 24 patients. Of 17 patients with stage A and B1(Gunderson - Sosin modification of Dukes' staging system) 6 patients experienced local recurrence (31.2%). The local recurrence rate of B2 and B3 group was 29.9% (7/33) and that of C2 and C3 was 54.7% (11/19), respectively. There was statistically significant difference between two groups (p<0.05). Of 23 patients treated with definitive surgery and radiation therapy 10% (1/10) recurred in B2 and B3 patients. This was slightly lower than C2 and C3 patients (22.2%, 2/10) of similar policy, but revealed no statistically significant difference (p>0.05). In the patients of B2+3 local failure rate decreased when radiation therapy was added (29.9%) vs 10%, p>0.05) and also similar results in C2+3 group (34.7% vs 22.2%, p<0.05). The local failure rate in relation to distance from the anal verge had verge had no statistically significant difference.

Inhibitory Effects of Ginseng Saponins on c-fos mRNA Expression and the Proliferation of Rat Aortic Vascular Smooth Muscle Cells Stimulated by Angiotensin II

Woong Choi,Jin Young Jung,Hun Sik Kim,Yeo Pyo Yun,Jong Dae Park.,Hee Yul Ahn 대한생리학회-대한약리학회 1998 The Korean Journal of Physiology & Pharmacology Vol.2 No.2

<P> To evaluate the possibility that the ginseng saponins could be developed as an anti-arteriosclerotic agent, we examined the inhibitory effects of ginseng saponins (total saponin[TS], panaxatriol[PT], panaxadiol[PD]) on the expression of c-fos mRNA and the proliferation of cultured rat aortic vascular smooth muscle cells (VSMCs) stimulated by angiotensin II (Ang II). TS and PT (1.0 mg/ml) suppressed c-fos mRNA induction in VSMCs stimulated by 10<SUP>-5</SUP> M Ang II. The order of inhibitory potency was PT>TS. Ginseng saponins (0.01∼1.0 mg/ml) inhibited the proliferation of VSMCs stimulated by Ang II in a concentration dependent manner, the inhibitory potency was TS>PT>PD at 0.1∼1.0 mg/ml. These results suggest that ginseng saponins may suppress Ang II-stimulated proliferation of aortic VSMCs which can be seen in atherosclerosis, hypertension and restenosis.

EU의 Biomass를 이용한 에너지 생산

이종식,고문환,박우균,안종웅 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.3

EU 국가들의 1993년부터 1999년까지의 6년간 재생에너지 사용량 변화를 살펴보기 위하여 절대량 증가와 증가율을 조사하였다. 많은 나라들이 높은 사용량 증가 또는 증가율을 나타내었다. 초기에 재생에너지 사용이 적었던 나라들은 1999년에도 실질적인 생산량은 적지만 기간 중 높은 증가율을 보였다. 반면에 1993년에 이미 사용량이 높았던 나라들은 낮은 증가율을 보였으나 전체사용량은 높은 수준이었다. 재생 에너지 사용의 성공 여부는 보급 확대를 위해 많은 장해물들을 극복한 후에 성취될 수 있다. 보급 확대를 위한 요인들로는 정치적, 제도적, 재정적, 행정적인 지원과 기술개발, 홍보, 교육 및 훈련 등이 있다. Biomass를 이용한 에너지 생산 및 보급에 성공한 덴마크, 핀란드, 독일, 스페인 및 스웨덴의 송공 요인들을 소개하였다. In order to identify those Member State/technology combinations where there has been most success in achieving renewable energy penetration over the six-year period 1993-1999, the two selection criteria such as absolute increase and percentage of increase have been applied. Many Member States show either a large absolute increase in renewable energy output, or a large percentage of increase. Member States with only very low initial levels of renewable energy use may demonstrate rapid growth rates even though the actual quantity of output is still only small. Conversely, Member State with levels of renewable energy use that were already relatively high in 1993 show a less rapid percentage growth rate, but these Member States may still Have added significant quantities to the overall output. Successful penetration of renewable energy can only be achieved after overcoming many varied obstacles to an increase in their exploitation. Factors, which can influence the successful implementation of renewable energy projects, include political, legislative, financial administration, technological development, information, education and training. Examples of successful penetration of biomass power were shown for Denmark, Finland, Germany, Spain and Sweden.

발작성 야간혈색소뇨증의 생존에 관한 연군

이영식,한지숙,이선주,조준구,안용정,고윤웅 大韓血液學會 1987 大韓血液學會誌 Vol.22 No.2

비인두암의 국소 종양 치유와 생존율에 관한 예후 인자 분석

정웅기(Woong-Ki Chung),조재식(Jae-Shik Cho),박승진(Seung Jin Park),이재홍(Jae-Hong Lee),안성자(Sung Ja Ahn),남택근(Taek Keun Nam),최찬(Chan Choi),노영희(Young Hee Noh),나병식(Byung Sik Nah) 대한방사선종양학회 1999 Radiation Oncology Journal Vol.17 No.2

목 적 : 비인두암 환자에서 항암화학요법과 방사선치료 후 국소종양제어율, 생존율, 무병생존율에 미치는 예후 인자를 알아보고자 하였다. 대상 및 방법 : 1986년 7월부터 1996년 6월까지 약 10년간 전남대학교병원에서 비인두암으로 확진되어 근치적 목적으로 치료를 받은 47명의 환자를 대상으로 후향적 분석을 시행하였다. 대상 환자의 연령 분포는 16세에서 80세까지였고 중앙 값은 52세였다. 성별 분포는 남자가 33명(70%), 여자가 14명(30%)이었다. WHO의 기준에 의한 조직학적 유형은 제1형(케 라틴형성 편평세포암)이 3례(6%), 제2형(비케라틴형성 편평세포암)이 30례(64%), 제3형(미분화암)이 13례(28%)였고 나머 지 1례(2%)는 조직학적 유형이 알려지지 않았다. 미국암합동위원회(1997)의 병기분류법에 따라 후향적으로 다시 분류한 병기는 T1, T2a, T2b, T3, T4에서 각각 11례(23%), 6례(13%), 9례(19%), 7례(15%), 14례(30%)였다. 그리고 림프절 침범 상태는 N0, N1, N2, N3에서 각각 7례(15%), 14례(30%), 21례(45%), 5례(10%) 있었다. 병기군별 분포는 Stage I, IIA, IIB, III, IVA, IVB에서 각각 2례(4%), 2례(4%), 10례(21%), 14 례(30%), 14례(30%), 5례(11%) 있었다. 방사선치료 전에 항암제 치료를 받은 환자는 42례이며 5례는 항암제 치료가 시행되지 않았다. 방사선치료는 선형가속기의 6MV와 10MV X-ray 및 9 MeV 전자선을 사용하였으며 원발 병소에 조사된 총방사선량은 6120- 7920cGy(중앙값: 7020cGy)였다. 항암화학요법은 Cisplatin+5-Fluorouracil(25명), Cisplatin+Pepleomycin(17명)으로 1회에서 3회까지 시행하였다. 국소종양제어율, 생존율, 무병생존율을 Kaplan-Meier법에 의하여 산출하였으며 두 군간의 생존율의 차이는 Generalized Wilcoxon test를 이용하여 검증하였다. 영향을 주는 인자의 다변량분석에는 Cox 모델을 이용하였다. 결 과 : 국소종양 제어율은 2년에 89%, 5년에 81%이었다. 5년 생존율은 60%(범위; 6-132개월, 중앙값; 32개월)이었다. 예후에 영향을 미치는 위험 인자로 연령, 성별, 두개신경침범, 병리조직학적 유형, 병기군, 항암화학요법, 항암화학요법과 방사선치료 사이의 간격, 방사선량, 방사선치료기간을 다변량분석에 포함시켰다. 국소종양제어율에는 두개신경침범(P=0.004)만이 의의 있는 것으로 나타났다. 생존율과 무병생존율에는 병기군(P=0.006, P=0.003)과 총방사선량(P=0.012, P=0.008)이 의의 있는 것으로 나타났다. 치료 후 합병증은 구강건조증, 치아손상, 이증상 등이 많았으며 2례의 갑상선기능저하증이 있었다. 결 론 : 비인두암에서 예후에 영향을 미치는 인자로서 국소종양제어율은 두개신경침범 여부가, 생존율 및 무병생존율에는 총방사선량 과 병기군, 특히 N 병기가 의의 있는 것을 알 수 있었다. 사용된 항암화학요법과 방사선치료는 심각한 부작용이 없이 효과적으로 이용될 수 있음을 알 수 있었다. Purpose : This study was performed to find out the prognostic fac tors affecting local control, survival and disease free survival rate in nasopharyngeal carcinomas treated with chemotherapy and radiation therapy. Materials and Methods : We analysed 47 patients of nasopharyngeal carcinomas, histologically confirmed and treated at Chonnam University Hospital between July 1986 and June 1996, retrospectively. Range of patients' age were from 16 to 80 years (median; 52 years). Thirty three (70%) patients was male. Histological types were composed of 3 (6%) of keratinizing, 30 (64%) of nonkeratinizing squamous cell carcinoma and 13 (28%) of undifferentiated carcinoma. Histological type was not known in 1 patient (2%). We restaged according to the staging system of 1997 American Joint Committee on Cancer. Forty seven patients we re recorded as follows: T1; 11 (23%), T2a; 6 (13%), T2b; 9 (19%), T3; 7 (15%), T4; 14 (30%), and N0; 7 (15%), N1; 14 (30%), N2; 21 (45%), N3; 5 (10%). Clinical staging was grouped as follows: Stage I; 2 (4%), IIA; 2 (4%), IIB; 10 (21%), III; 14 (30%), IVA ; 14 (30%) and IVB; 5 (11%). Radiation therapy was done using 6 MV and 10 MV X- ray of linear accelerator. Electron beam was used for the lymph nodes of posterior neck after 4500 cGy. The range of total radiation dose delivered to the primary tumor was fro m 6120 to 7920 cGy (median; 7020 cGy). Neoadjuvant chemotherapy was performed with cisplatin+5-fluorouracil (25 patients) or cisplatin +pepleomycin (17 patients) with one to three cycles. Five patients have not received chemotherapy. Local control rate, sur vival and disease free survival rate were calculated by Kaplan- Meier method. Generalized Wilcoxon test was used to evaluate the difference of survival rates between groups. Multivariate analysis using Cox proportional hazard model was done for finding prognostic factors. Results : Local control rate was 81% in 5 year. Five year survival rate was 60% (median survival; 32 months). We included age, sex, cranial nerve deficit, histologic type, stage group, chemotherapy, elapsed days between chemotherapy and radiotherapy, total radiation dose, period of radiotherapy as potential prognostic factors in multivariate analysis. As a result, cranial nerve deficit (P=0.004) had statistical significance in local control rate. Stage group and total radiation dose were sig nificant prognostic factors in survival (P=0.006, P=0.012), and in disease free survival rates (P=0.003, P=0.008), respectively. Common complications were xerostomia, tooth and ear problems. Hypothyroidism was developed in 2 patients. Conclusion : In our study, cranial nerve deficit was a significant prognostic factor in local control rate, and stage group and total radiation dose were significant factors in both survival and disease free survival of nasopharyngeal carcinoma. We have concluded that chemothe rapy and radiotherapy used in our patients were effective without any serious complication.

Long-Term Clinical Outcome of Lamivudine Monotherapy in HBeAg Positive Patients with Chronic Hepatitis B

( Hyun Woong Lee ),( Kwang Hyub Han ),( Sung Min Myung ),( Yun Hee Chung ),( Sang Hoon Ahn ),( Ja Kyung Kim ),( Yong Han Paik ),( Kwan Sik Lee ),( Chae Yoon Chon ),( Young Myoung Moon ) 대한간학회 2006 Clinical and Molecular Hepatology(대한간학회지) Vol.12 No.3(S)