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Helicobacter pylori와 대장균의 Shuttle Vector 개발
조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-
In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.
Assessing Renal Ischemia/Reperfusion Injury in Mice Using Time-Dependent BOLD and DTI at 9.4 T
Woo, Dong-Cheol,Kim, Nayoung,Lee, Do-Wan,Song, Youyol,Shim, Woo Hyun,Choi, Yoonseok,Woo, Chul-Woong,Kim, Sang-Tae,Kim, Jeong-Kon,Kim, Kyungwon,Ha, Hyun-Kwon,Lee, Jin Seong Springer-Verlag 2015 Applied magnetic resonance Vol.46 No.6
( Woo Kon Jeong ),( Myung Ho Jeong ),( Kye Hun Kim ),( Sang Rok Lee ),( Ok Young Park ),( Ju Hyup Yum ),( Joo Han Kim ),( Won Kim ),( Jae Young Rhew ),( Youn Keun Ahn ),( Jeong Gwan Cho ),( Byoung Hee 대한내과학회 2003 The Korean Journal of Internal Medicine Vol.18 No.3
Background: The current techniques for percutaneous coronary interventions (PCI) remain limited by restenosis. Recent studies have provided evidence of inflammation playing a role in the pathogenesis of cardiovascular disease. Methods: Whether inflammator
High-Speed and Low-Complexity Decoding Architecture for Double Binary Turbo Code
KWON, Kon-Woo,BAEK, Kwang-Hyun,LEE, Jeong Woo The Institute of Electronics, Information and Comm 2011 IEICE transactions on fundamentals of electronics, Vol.ea94 No.11
<P>We propose a high-speed and low-complexity architecture for the very large-scale integration (VLSI) implementation of the maximum <I>a posteriori</I> probability (MAP) algorithm suited to the double binary turbo decoder. For this purpose, equation manipulations on the conventional Linear-Log-MAP algorithm and architectural optimization are proposed. It is shown by synthesized simulations that the proposed architecture improves speed, area and power compared with the state-of-the-art Linear-Log-MAP architecture. It is also observed that the proposed architecture shows good overall performance in terms of error correction capability as well as decoder hardware's speed, complexity and throughput.</P>
Appropriate diagnosis of biliary cystic tumors: comparison with atypical hepatic simple cysts
Seo, Jeong Kyun,Kim, Su Hyun,Lee, Sang Hyub,Park, Joo Kyung,Woo, Sang Myung,Jeong, Ji Bong,Hwang, Jin-Hyeok,Ryu, Ji Kon,Kim, Jin-Wook,Jeong, Sook-Hyang,Kim, Yong-Tae,Yoon, Yong Bum,Lee, Kuhn Uk,Kim, S Lippincott Williams Wilkins, Inc. 2010 European journal of gastroenterology & hepatology Vol.22 No.8
BACKGROUND: Biliary cystadenoma (BCA) and biliary cystadenocarcinoma (BCAC) are often confused with other intrahepatic cystic diseases. AIMS: The aims of this study were to investigate predictive factors of biliary cystic tumor (BCT) and clinical characteristic of BCAC. METHODS: We retrospectively reviewed preoperative diagnoses, overall characteristics and postoperative outcome of 20 BCTs and 19 cystadenoma-mimicking simple cysts that were pathologically confirmed. RESULTS: Comparing with atypical simple cysts, symptoms, left-lobe cyst, thick wall, septation, mural nodule, bile duct dilatation and an increase of serum alkaline phosphatase were associated with BCTs. However, on multivariate analysis, mural nodule, left-lobe cyst, and an increase of serum alkaline phosphatase were significantly frequent in BCTs with odds ratios of 75.5, 13.8, and 33.0, respectively. Among the 20 BCTs, seven BCACs were diagnosed. The characteristics of BCACs were mural nodule (P<0.01), intrahepatic cyst debris (P<0.01), and bile duct dilation (P=0.04). Cystic fluid analysis provided no significant differences between BCT and simple cyst. After fine needle aspiration cytology of BCTs, all except one BCAC with atypical cell showed nonspecific findings. After complete surgical excision (97.4% of patients), only one patient with BCAC had recurrence during 29 months of follow-up period. CONCLUSION: In hepatic cysts with mural nodule, left-lobe cyst or increment of serum alkaline phosphatase seem to be indicative of BCTs in the diagnosis of suspicious hepatic cyst. Intracystic debris, bile duct dilation, and mural nodule may be suggestive clinical features of malignancy in BCTs.
증가된 C - Reactive Protein 은 관상동맥 중재술 후 재협착에 대한 유일한 예측인자
정우곤(Woo Kon Jeong),정명호(Myung Ho Jeong),김계훈(Kye Hun Kim),이상록(Sang Rok Lee),박옥영(Ok Young Park),염주협(Ju Hyup Yum),김원(Won Kim),김주한(Ju Han Kim),류제영(Jae Young Rhew),안영근(Youn Keun Ahn),조정관(Jeong Gwan Cho),안병희 대한내과학회 2002 대한내과학회지 Vol.62 No.4
연구배경: 최근에 동맥경화 및 관상동맥 질환의 발생의 중요한 역할을 하는 감염 및 염증인자에 대한 연구가 활발히 이루어지고 있다. 본 연구에서는 관상동맥 중재술 후 발생하는 재협착과 염증인자의 상관관계 및 CMV, C. pneumoniae, H. pylori 등의 감염과 상관관계에 대해서 전향적 조사를 하였다. 대상 및 방법: 1999년 9월부터 2001년 3월까지 처음으로 관상동맥 질환으로 진단된 후 관상동맥 중재술을 시행한 후 추적 관상동맥 조영술을 시행받았던 272예 중 재협착이 발생하였던 재협착군 (99명, 134병변, 59.5±10.8세)과 재협착이 발생하지 않았던 비재협착군 (173명, 211병변, 58.8±10.2세)으로 나누어, 양군간의 세 가지 감염항체의 양성률 및 역가와 CRP를 측정하여 재협착과 연관성 등을 전향적으로 분석하였다.결과: 재협착군과 비재협착군에서 C. pneumoniae, H. pylori, CMV의 항체 양성률은 각각 27.3%와 26.0%, 61.6%와 63.6%, 100%와 100%로서 양군간에 유의한 차이는 없었다. CRP의 양성률은 재협착군과 비재협착군에서 각각 57.6%와 36.4%로서 재협착군에서 유의하게 높았고 (p=0.001), CRP의 측정치는 3.38±5.80 mg/dL와 1.36±2.62 mg/dL로서 재협착군에서 유의하게 높았다 (p=0.001). 관상동맥 재협착 예측인자에 대한 다변량 회귀 분석에서 CRP는 상대위험도 2.1169, 95% 신뢰구간 1.2062∼3.7154 (p=0.009)로서 재협착 발생에 대한 유일한 독립적인 위험인자이었다. 결론: 추적 관상동맥 조영술상 재협착군과 비재협착군에서 C. pneumoniae, H. pylori, CMV의 항체 양성률은 양군간에 유의한 차이는 없었고 CRP의 양성률, 측정치 모두 유의하게 높았고, CRP는 재협착 발생을 예측할 수 있는 유일한 인자이었다. Background: Current techniques of percutaneous coronary interventions (PCI) remain limited by the restenosis. Recent studies provide evidence that inflammation plays a role in the pathogenesis of cardiovascular disease. Methods: We prospectively tested whether inflammatory markers are predictors of subsequent restenosis in 272 consecutive patients with angiographically proved coronary artery disease. The patients who underwent PCI at Chonnam National University Hospital between Sep. 1999 and Mar. 2001 were divided into two groups according to the occurrence of restenosis on follow-up coronary angiogram: patients with restenosis (Group I: n=99, 59.5×10.8 years, M:F=77:22) and patients without restenosis (Group II: n=173, 58.8×10.2 years, M:F=131:42). IgG seropositivity and titer of CMV, C. pneumoniae, H. pylori, levels of C-reactive protein (CRP) were compared between two groups. Results: There were no statistical differences in the seropositivity of CMV IgG, C. pneumoniae IgG, H. pylori IgG between two groups (Group I vs. II: 100% vs. 100%, 24.7% vs. 25.7%, 62.2% vs. 63.7% in group I vs. II respectively). Among angiographic parameters, low TIMI flow (TIMI 0 or I) was more common in Group I than in Group II (p=0.038). The patients with elevated CRP (>0.5 mg/dL) were more common in Group I than those in Group II (57.6% vs. 36.4%, p=0.001) and the value of CRP was higher in Group I than in Group II (3.3±5.8 mg/dL vs. 1.3±2.6 mg/dL, p=0.001). According to multiple logistic regression analysis, CRP was the only predictor of restenosis with odd ratio of 2.1169 (95% C.I. 1.2062-3.7154, p=0.009). Conclusion: The value of CRP is the most important predictor of restenosis after PCI. (Korean J Med 62:405-414, 2002)