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Cen Qian,Kunzhang Lin,Baojian Zhu,Chao-Liang Liu,Lei Wang,Ya Liu,Dan Liang,PeiWang,Guo-Qing Wei 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
C-Type lectins (CTLs) are carbohydrate-recognition proteins and play critical roles in innate immunity. In this study, we characterized a CTL fromAntheraea pernyi, named ApCTL,which has two carbohydrate-recognition domains (CRDs). A fragment of 1017 bp cDNA of ApCTL was isolated, which constituted 927 bp open reading frame (ORF), and it encoded putative protein of 308 amino acids. The recombinant protein of ApCTL was expressed in Escherichia coli, and anti-ApCTL antibodieswere prepared. ApCTL genewas constitutively expressed in a variety of tissueswhile it showed a higher expression level in fat body than other tissues. Protein andmRNAlevels of ApCTL in fat body and hemolymph were elevated upon nuclear polyhedrosis virus (NPV) and E. coli challenges. Recombinant ApCTL increased the agglutination of E. coli in a Ca2+-dependent manner and had the bacteriolytic activity on E. coli. Our results suggested that ApCTLmay play critical roles in pathogen recognition and could be involved in the innate immunity of A. pernyi.