간접 혈구응집반응(IHA)을 이용한 간흡충증의 혈청학적 진단에 관한 연구

김태성,주경환,임한종 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.1

Detection of infections with Clonorchis sinensis has been accomplished through various serodiagnostic procedure including complement fixation test, gel diffusion test, indirect fluorescent antibody test and enzyme linked immunosorbent assay etc. In 1960, Pacheco, et al. reported that indirect hemagglutination test showed relatively more sensitive reaction than CFT in experimentally infected animal and 3 human cases. However, more detailed studies on the use of IHA in serodiagnosis of clonorchiasis were not accomplished. Evaluation of an indirect hemagglutination test was made in 100 egg positive cases in comparison with 51 parasite infected cases other than C. sinensis and 58 parasite free control. Kagan's modification of Boyden technique was employed using ether treated borate buffer extract adult worm antigen with 0.5㎎/ml concentration by Chaffee's method. The results obtained were as follows: 1. Ether treated borate buffer extract adult worm antigen was more sensitive than that of veronal buffered salt extract. 2. Sensitivity of IHA test was 84.0%) among 100 Clonorchs egg positive cases when cut-off titer of 1:16 was applied. Only 1 control sera out of 58 cases(98.3%) specificity) showed positive reaction. 3. Sensitivity was only 80.0% in light infection, 87.1% in moderate infection. However, only 1 case showed false negative reaction among 14 cases in heavy and very heavy infection category. 4. ELISA was more sensitive than IHA test, showing 90.0% sensitivity and 98.3% specificity. 5. Only 2 cases infected with Paragonimus westermani showed positive cross reaction in either IHA test or ELISA. 6. A positive correlation was found between results of ELISA and IHA test of patients with clonorchiasis. And the results obtained in both techniques were same in 86% of positive sera and 100% of negative sera. In conclusion, it is suggested that in addition to ELISA, IHA is a relatively sensitive and reliable test for the sere-diagnosis and sero-epidemiological survey of C. sinensis infection.

음식물 섭취에 따른 요중 마뇨산 배설량의 변화

김정일,박태혁,정갑열,신해림,김준연 동아대학교 산업의학연구소 2000 산업의학연구소 논총 Vol.- No.5

Objectives ; Urinary hippuric acid levels have been usually determined to evaluate workers exposes to toluene. Food and drink intake could affect urinary hippuric acid excretion and chi study was carried out to investigate what kinds of food and drink would affect urinary hippuric acid excretion. Methods ; The subjects were 136 medical school students. Authors measured urinary hippuric acid levels three times from 10 a. m. to noon; before, one hour after and two hours after food or drink-intake, and they were asked not to eat from the previous night(9 p. m) of testing. We selected six diets(bread and coffee, brown sauce noodle, Korean style meal, noodle, pork-galbi and rice and soybean stew and rice) , six fruits(apple, grape, orange, pear, persimmon and plum) and five beverages(Alps-D□, Demisoda□, Orange juice□, Pear juice□ and Pocari sweat□). Analysis of urinary hippuric acid was performed by high performance liquid chromatography. Results ; Intake of popular Korean diets, fruits and some drinks didn't affect urinary hippuric acid excretion. But intake of sodium benzoate-contained Alpa-D□ increased urinary hippuric acid excretion. Conclusions ; For the health examination on workers exposed to toluene, the history of diet and fruit intake can be negligible, but that of beverage intake should be included.

임플란트 식립 방향에 따른 상부구조물의 정확도 차이에 관한 비교연구

김태석,임재석 고려대학교 임프란트연구소 2001 임프란트연구지 Vol.3 No.1

The gap discrepancies between cast implant superstructure supported by two fixtures aligned without puntic and fixture replica were measured utilizing stereomicroscope with the assumptions that the direction of two fixtures was parallel or in convergent relation(20˚). The results were as follews : 1.The gap discrepancies measured between the replica and implant superstructure cast with plastic burn-out cylinder using non-precious alloy were 6.04±1.07㎛. 2.The gap discrepancies of non-parallel replica were 119.24±15.73㎛(Mean±SD) and that of parallel replica were 87.02±17.54㎛(Mean±SD), which show statistically significant difference (P〈0.001).

대식세포주 RAW264.7에서 Nitric oxide에 의해 과발현되는 CD53의 기능분석

김태림,이혜영,김인규,백상기 충남대학교 생물공학연구소 2004 생물공학연구지 Vol.10 No.2

The CD53 antigen is a member of the tetraspanin membrane protein family that is expressed in the lymphoid-myeloid lineage. It is highly expressed in Radio-resistant tumor cells. Recently, it was reported that CD53 associates with the GSH-metabolizing protein γ-glutamyl transpeptidase. Its biological roles however remains unknown. Macrophages activated by microbial lipopolysaccharides (LPS) produce a burst of nitric oxide and reactive oxygen species. Nitric oxide plays important roles in macrophage activation as a toxic agent towards infectious organisms, an inducer or suppressor of apoptosis or an immunoregulator, whether nitric oxide can induce or inhibit apoptosis is highly dependent on different cell types at different stages of the inflammatory process. Using cDNA microarrays, we identified CD53 as one of the principal genes up-regulated by exposure of macrophages to LPS. We found that mRNA and protein levels of CD53 were increased by nitric oxide as well as LPS treatment in macrophages. Cells stably transfected with sense CD53 cDNA had lower levels of peroxide, and were more resistant to γ-irradiation. The stably transfected RAW264.7 cells with antisense CD53 recombination had the opposite properties. Activation of CD53 by cross-linking with monoclonal anti-CD53 antibody (OX-79) showed the expression of the inducible nitric oxide synthase. We propose that the induction of CD53 is one of major self-protection mechanisms of macrophages against LPS induced-oxidative stress and irradiation.

생소한 HBsAg 선별검사키트 bioelisa와 IEMA Well의 평가

김덕언,김홍석,김신규,이혜림,박일규,강정옥,최태열 대한임상검사정도관리학회 1997 臨床檢査와 精度管理 Vol.19 No.2

한랭노출과 고혈압의 연관성

장태원,김윤규,윤동영,이창희,홍영습,신해림,정갑열,김준연 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.4

Objectives : This study was carried out to suggest preventive methods for hypertension in cold-exposed workers. Methods : In 11 refs·iteration industries, 68 workers working in refrigerated areas more than one time per day were selected as the exposed group, and 68 workers not exposed to cold were selected as the control group. We interviewed the subjects with a questionnaire covering occupational history, and conducted clinical and laboratory tests including measurements of blood pressure and core temperature. Results : The systolic blood pressure in the exposed group(130.0±13.3 mmHg) was significantly higher than that recorded In the control group(118.3±12.1 mmHg), as was the diastolic blood pressure in the exposed group(82.7±8.5 mmHg) versus the control group(77.4±8.7 mmHg). The core temperature in the exposed group(36.1±0.7℃) was significantly lower than that experienced in the control group (36.4±0.5℃). In logistic regression analysis, age, cold exposure severity and milk intake were significant variables, with odds ratios of 5.204(95 % CI 1.440∼18.812), 2.674(95 % CI 1.080∼6.618), and 0.364(95 % Cl 0.141∼0.942) , respectively. Conclusions : Our study suggests that cold-exposed workers have higher a higher risk of hypertension, and that their core temperature is lower. Risk factors affecting hyper-tension of cold-exposed workers include age, cold exposure severity and milk intake. For the prevention of hypertension, cold-exposed workers should minimize cold-exposure time as much as possible.

플라스틱 V-벨트 풀리 설계 및 시작품 제작에 관한 연구

손태일,임재규,김형종 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.B

In this study, product design and prototype manufacturing of a plastic water-pump pulley has been tried. The designed model is supposed to be made of 33% glass reinforced resin of which the tensile strength is 180 MPa, and has 24 ribs on each side to increase its structural strength. Structural analysis under a static load of 300kgf acting on both edges of the belt has been carried out using a commercial finite element code, MARC. The analysis result showed the maximum effective stress near a rib of designed model would be at must 35MPa (less than 20% of the tensile strength), therefore, the plastic product would be sufficiently safe under that loading condition. On the basis of the structural analysis, a prototype of the designed model has been manufactured by using the fused deposition modelling (FDM) method which is one of the rapid prototyping (RP) methods, using ABS resin and support materials. The CAD data exported to the RP system in STL format was prepared by a commercial solid modeling soft ware, SolidWorks. It has been proved that the plastic pulley can successfully replace the existing flow-farmed steel product.

항진균물질을 생산하는 Streptomyces fimbriatus AF21-3의 분리 및 동정

장주현,김정완,이우윤,윤영준,이경림,김승곤,이태수 인천대학교 2000 基礎科學論文集 Vol.11 No.1

Actinomycetes were isolated from soil to find new antibiotics. An active antimicrobial compound was isolated from the culture filtrate of Actinomycetes AF 21-3. The Actinomycetes strain which showed strong antifungal activity against Candida tropicalis and Microsporum gypseum was identified as Streptomyces fimbriatus and named as Streptomyces fimbriatus AF 21-3.

Propylthiouracil에 의한 중증 급성간염 1예

임종주,심미령,이명수,김태현,오석규,안선호,박무림,김남호,박병현,조성구 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.2

PTU에 의한 간염의 발생은 매우 드물게 발생하는 것으로 알려져 있으나 저자들은 Graves씨 병으로 진단 받고 propylthiouracil 투여를 받고있던 환자에서 중증의 급성 간염이 발생한 1예를 경험하였기에 보고하는 바이다. Propylthiouracil is widely used to treat patients with hyperthyroidism. This drug has been associated with severe hepatotoxicity rarely. We presented the case of jaundice and profound liver dysfunction from a 23-year old woman treated with propylthiouracil for hyperthyroidism. Viral, metabolic, and autoimmune liver disease could be excluded and liver biopsy revealed a pattern of acute hepatitis. After discontinuing the drug, there was a progressive resolution of hepatic symptoms and decrease in biochemical data of the liver. Despite propylthiouracil induced hepatitis in rare case, patients receiving propylthiouracil are exposed to develope severe hepatotoxicity. Therefore it might be advisable to monitor level of the transaminase on regular base from patients receiving propylthiouracil.

Fabrication of Bi-2212/ Composite Superconductors by Melting Powder Mixtures

Kim Kyu-Tae,Jang Seok-Hern,Lim Jun-Hyung,Park Eui-Cheol,Joo Jin-Ho,Lee Hoo-Jeong,Hong Gye-Won,Kim Chan-Joong,Kim Hye-Rim,Hyun Ok-Bae 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1

We fabricated Bi-2212/ composite superconductors and evaluated the effects of the powder mixing method and melting temperature on their microstructure and superconducting properties. The Bi-2212 powders were mixed with by hand-mixing (HM) and planetary ball milling (PBM) and then the powder mixtures were melted at , solidified, and annealed. We found that the powder mixture prepared by PBM was finer and more homogeneously mixed than that prepared by HM, resulting in more homogeneous microstructure and smaller and second phases after annealing.