大豆에서 水分스트레스에 依한 抗酸化酵素의 活性度 變化

朴愚喆,姜相載,金兌性 慶北大學校農業科學技術硏究所 1998 慶北大農學誌 Vol.16 No.-

水分 스트레스에 의한 植物體의 防禦機構와 關聯된 superoxide dismutase와 catalase의 活性度 變化를 調査한 結果는 다음과 같다. 水分 스트레스(drought, flooding) 處理時 큰올콩과 은하콩 모두 水分 含量과 可溶性 蛋白質 含量이 減少하여 末成熟 老化를 나타내었다. 可視的인 被害는 drought 處理時가 flooding 處理에 比해 크게 나타났고 큰올콩이 은하콩에 比해 크게 나타났다. superoxide dismutase의 活性은 drought處理時 2日째에 急激한 減少를 보이다가 回復되고 다시 減少하는 傾向을 보였으며 flooding處理時는 處理期間에 따라 漸次 減少하였고 큰올콩의 減少程度가 더 크게 나타났다. catalase의 活性은 drought處理時는 減少하는 傾向이었으나 flooding處理時 2日째에 急激한 減少를 나타낸 후 一定하게 維持되는 傾向을 보였다. 水分 스트레스 處理後 3日間의 回復期를 주었을 때 두 酵素의 活性은 漸次 回復되었다. This experiment was carried out to elucidate and study about plant defense mechanism subjected to water stress(drought, flooding). We measured water content, total soluble protein content as stress marker and superoxide dismutase(SOD), catalase (CAT) as antioxidant enzymes subjected to water stress(drought, flooding) and recovery in soybean leaves. The results obtained were as follows; Two soybean lines(keonolkong, euhakong)leaves exposed to water stress (drought, flooding) showed premature senescence as evidence by the decrease in water content, and total soluble protein content, but those of soybean leaves subject to water stress recovery for 3 days were recovered. Visual damage was much worse at drought stress than flooding stress and was worse keunolkong than enhakong. The activity of superoxide dismutase, catalase subjected to water stress(drought, flooding)was on the decrease, but degree of decrease was different from a sort of soybean lines, drought and flooding stress.

주의력결핍과잉행동장애와 Alpha-1C-Adrenergic 수용체 유전자와의 연관성에 대한 연구

조수철,김재원,김붕년,황준원,박미라,김순애,조대연,유희정,정운선,손정우,박태원 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.1

Objectives : Neurobiological and pharmacological research has suggested that the dysregulation of the central noradrenergic systems might be involved in the pathophysiology of ADHD. The aim of this study was to examine the association of the alpha-1C-adrenergic receptor gene (ADRA1C) with ADHD in Korean subjects. Methods : In a case-control study, we assessed 186 DSM-IV ADHD probands and 150 normal controls. One hundred and eight trios were studied in a family-based association analysis. Psychiatric diagnoses were derived through structured diagnostic interviews. For the clinical evaluation of the ADHD subjects, the Child Behavior Checklist (CBCL), the ADHD Rating Scale-IV (ARS) and the Junior Temperament and Character Inventory (JTCI) were administered. A computerized continuous performance test (CPT) was used to measure the inattention and impulsivity of the ADHD children. Results : There were no significant differences in the genotype or allele frequencies of the ADRA1C PstI polymorphism between the ADHD and control group (p>0.05). The transmission disequilibrium test (TDT) analysis observed no evidence for biased transmission of any of the alleles of the PstI polymorphism. There were no significant differences in the CPT or JTCI profiles between those ADHD subjects with the CC genotype and those with the other (CT+TT) genotypes at the PstI polymorphism. Conclusion : The results of this study do not support the ADRA1C as a major genetic susceptibility factor in ADHD.

石灰 및 苦土가 土壤內 有效成分變化와 水稻生育에 미치는 影響

崔元凱,文泰萬,河浩成,朴愚喆 진주농과대학 1968 진주농과대학 연구논문집 Vol.- No.7

pH 5.5의 耕作土壤에 石灰를 1-6me/100gr까지 增加시켜 土壤內 成分과 水稻生育에 對하여 몇 가지 結果를 얻었으며, 역시 同一土壤에 Mg를 1-4me/100gr 施用하여 몇 가지 結果를 얻었다. (1) Ca當量別 增加에 따라 exch-Ca와 pH의 變化는 比例的으로 增加되어 高度의 相關이 있다. (2) 有效燐酸, 可溶性SiO2, 有機物, 活性鐵, 置換性 苦土等의 含量變化는 認定되지 않았다. (3) 燐酸의 吸收係數는 Ca施用에 따라 正 相關이 認定된다. (4) Ca施用에 따라 生育面에 莖數, 穗數는 減少되어 負相關이 認定된다. (5) 草長, 稈長, 稔實率, 穗當着粒數에는 變化가 認定되지 않는다. (6) Mg를 1-4me/100gr 增加施用에 따라 土壤內 exch-Mg와 有效燐酸은 增加되었다. (7) pH 및 基他成分은 變化가 나타나지 않았다. (8) Mg施用에 따라 生育은 草長, 莖數는 增加가 없었고 穗數와 稈長이 增加된 傾向이고 基他는 變化가 없다. Some results of rice growth and available element in soil were obtained by application 1-6me/100gr of calcium and 1-4me/100gr of magnessium in a soil which was pH 5.5. The results of the study showed the followings. 1. Increasing the application of calcium, exch-Ca, pH and absorption coefficient of phosphorus were increased proportionally, but available ??, soluble SiO₂, O.M., active Fe and exch-Mg were not changed. 2. Number of Penicles and tillers were decreased proportionally but plant height, the length of culm, rate of grain formation and number of grain per penicle were not changed. 3. Increasing the application of magnessium, exch-Mg and available ?? were increased but the others were not changed. 4. Plant height and number of penicles were not increased but the number of tillers and the length of culm had tendency to increase.

대류난방시 상하온도차가 인체의 온열 쾌적성에 미치는 영향

김동규,김광호,김경철,박태안,박희욱 동의공업대학 2000 論文集 Vol.26 No.1

Purpose of this study is first to show the effects of vertical air temperature differences on thermal comfort and physiological responses, and second to examine the acceptable vertical air temperature differences. In this experiments, 12 subjects (6 males and 6 females) were exposed at 12 test conditions. The subjects voted each 10 min, about his thermal feeling, and 7 skin surface temperature, the air temperature were measured every 20 second. From this experiments, it was found that the upper part temperature affects the thermal sensation more strongly than lower part temperature. In winter, the acceptable temperature differences drawn by this study are 3℃ in the case that the upper part temperature is higher than the lower part temperature.

새로운 isoquinoline 유도체, AQ44의 혈관평활근 수축에 미치는 영향

김보경,김윤선,용준환,장병준,박태규,조성일,장기철,이상목 한국노화학회 1998 한국노화학회지 Vol.8 No.3

The novel anti-adipogenic effect and mechanisms of action of SGI-1776, a Pim-specific inhibitor, in 3T3-L1 adipocytes

PARK, YU-KYOUNG,HONG, VICTOR SUKBONG,LEE, TAE-YOON,LEE, JINHO,CHOI, JONG-SOON,PARK, DONG-SOON,PARK, GI-YOUNG,JANG, BYEONG-CHURL UNKNOWN 2016 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.37 No.1

<P>The proviral integration site for moloney murine leukemia virus (Pim) kinases, consisting of Pim-1, Pim-2 and Pim-3, belongs to a family of serine/threonine kinases that are involved in controlling cell growth and differentiation. Pim kinases are emerging as important mediators of adipocyte differentiation. SGI-1776,an inhibitor of Pim kinases, is widely used to assess the physiological roles of Pim kinases, particularly cell functions. In the present study, we examined the effects of SGI-1776 on adipogenesis. The anti-adipogenic effect of SGI-1776 was measured by Oil Red O staining and AdipoRed assays. The effect of SGI-1776 on the growth of 3T3-L1 adipocytes was determined by cell count analysis. The effects of SGI-1776 on the protein and mRNA expression of adipogenesisrelated proteins and adipokines in 3T3-L1 adipocytes were also evaluated by western blot analysis and RT-PCR, respectively. Notably, SGI-1776 markedly inhibited lipid accumulation during the differentiation of 3T3-L1 preadipocytes into adipocytes. On a mechanistic level, SGI-1776 inhibited not only the expression of CCAAT/enhancer-binding protein-alpha (C/EBP-alpha), peroxisome proliferator-activated receptor-gamma (PPAR-gamma) and fatty acid synthase (FAS), but also the phosphorylation of signal transducer and activator of transcription-3 (STAT-3). Moreover, SGI-1776 decreased the expression of adipokines, including the expression of leptin and regulated on activation, normal T cell expressed and secreted (RANTES) during adipocyte differentiation. These findings demonstrate that SGI-1776 inhibits adipogenesis by downregulating the expression and/or phosphorylation levels of C/EBP-alpha, PPAR-gamma, FAS and STAT-3.</P>

Anti-adipogenic Effect and Mechanism in 3T3-L1 Preadipocytes by Cyclosporin A, an Immunosuppressant

Tae-Yun Lee,Yu-Kyoung Park,Byeong-Churl Jang 계명대학교 자연과학연구소 2018 Quantitative Bio-Science Vol.37 No.1

Adipogenesis, also called the differentiation of preadipocytes, leads to the phenotype of mature adipocytes. However, excessive adipogenesis is closely linked to the development of obesity. Cyclosporin A, a neutral lipophilic cyclic undecapeptide isolated from the fungus Hypocladium inflatum gams, is known for several biological activities, including immunosuppression and anti-inflammation. Little is known about the relationship between cyclosporin A and obesity. In this study, we investigated the effect of cyclosporin A on adipogenesis in 3T3-L1 murine preadipocytes. 3T3-L1 preadipocyte differentiation was evaluated by Oil Red O staining and AdipoRed assay. The expression and phosphorylation levels of adipogenesis-related proteins in differentiating 3T3-L1 cells were evaluated by Western blotting. The mRNA expression levels of adipogenesis-related proteins in differentiating 3T3-L1 cells were measured by RT-PCR. Cyclosporin A considerably inhibited lipid accumulation and reduced triglyceride (TG) contents during the differentiation of 3T3-L1 preadipocytes into adipocytes in a concentration-dependent manner, suggesting its anti-adipogenic effect. Treatment with cyclosporin A at the concentrations tested was not cytotoxic to 3T3-L1 cells. Mechanistically, at a 10 μM concentration, cyclosporin A strongly down-regulated the protein and mRNA expressions of two adipogenic transcription factors, CCAAT/enhancer-binding protein-α (C/EBP-α) and peroxisome proliferator-activated receptor-γ (PPAR-γ) in differentiating 3T3-L1 cells. Furthermore, at a 10 μM concentration, cyclosporin A reduced the phosphorylation levels of signal transducers and activator of transcription-3 (STAT-3), other adipogenic transcription factor, and suppressed the protein and mRNA expressions of perilipin A, a protein that binds to and stabilizes lipid droplets, in differentiating 3T3-L1 cells. However, treatment with 10 μM of cyclosporin A did not modulate the protein and mRNA expressions of fatty acid synthase during 3T3-L1 preadipocyte differentiation. Collectively, these results show that cyclosporin A inhibits adipogenesis in differentiating 3T3-L1 cells, and that this inhibition is mediated through the reduced expression and phosphorylation levels of C/EBP-α, PPAR-γ, perilipin A, and STAT-3.

Anti-Adipogenic Effects on 3T3-L1 Cells and Zebrafish by Tanshinone IIA

Park, Yu-Kyoung,Obiang-Obounou, Brice Wilfried,Lee, Jinho,Lee, Tae-Yun,Bae, Myung-Ae,Hwang, Kyu-Seok,Lee, Kyung-Bok,Choi, Jong-Soon,Jang, Byeong-Churl MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.10

<P>Tanshinone IIA is a diterpene quinone isolated from the roots of <I>Salvia miltiorrhiza</I> bunge that has traditionally been used in China for the treatment of cardiovascular and cerebrovascular disorders. Although there is recent evidence showing that tanshinone IIA has an anti-obesity effect, its underlying mechanism of anti-obesity effect is poorly understood. Here, we investigated the effect of tanshinone IIA on lipid accumulation in 3T3-L1 preadipocytes and zebrafish. Notably, tanshinone IIA at 10 μM concentration greatly reduced lipid accumulation and triglyceride (TG) contents during 3T3-L1 preadipocyte differentiation, suggesting its anti-adipogenic effect. On mechanistic levels, tanshinone IIA reduced the expression levels of CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS), and perilipin A but also the phosphorylation levels of signal transducer and activator of transcription-3/5 (STAT-3/5) in differentiating 3T3-L1 cells. In addition, tanshinone IIA strongly inhibited leptin and resistin mRNA expression in differentiating 3T3-L1 cells. Importantly, the tanshinone IIA’s lipid-reducing effect was also seen in zebrafish. In sum, these findings demonstrate that tanshinone IIA has anti-adipogenic effects on 3T3-L1 cells and zebrafish, and its anti-adipogenic effect on 3T3-L1 cells is largely attributable to the reduced expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3/5.</P>

한국인 주의력결핍 과잉행동장애와 도파민 D5 수용체 유전자 다형성의 관련성 : 가족기반 연구 및 환자-대조군 연구

박태원,김붕년,임명호,유희정,강대희,조수철,유일한,조은정,백소영,배치운,서영주 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.1

Objectives : Recent genetic studies have suggested a preferential transmission of the Dopamine D5 receptor gene (DRD5) 148bp marker allele. The aim of this study is to test the association between DRD5 and ADHD. Methods : 106 Korean children with ADHD and their parents were analyzed using the transmission disequilibrium test (TDT) and haplotype-based haplotype relative risk test (HHRR). And also the ADHD children were compared with 212 ageand gender matched normal controls. Results : We found the evidence for an association of short alleles of DRD5 dinucleotide repeat polymorphism in both Case-control and family based studyies. Additionally, we observed some evidence for biased transmission of allele 152 bp and 144 bp. Conclusion : Our results lend credence to the notion that the relationship between ADHD and DRD5 is complex. The number of cases and informative transmissions in our study were small, therefore it would be premature to make any conclusions Con-ceming the role of DRD5 in ADHD. Further work is needed to support these findings.

소아 류마티양 관절염의 1례

박태규,이병철,이재청,차상덕 金原出版 1975 小兒科 Vol.16 No.5