녹두의 발아과정중 CA3 및 ABA 의 처리가 Peroxidase, Catalase 활성변화와 Isoperoxidase Pattern 에 미치는 영향

박우철,이상갑 한국콩연구회 1989 韓國콩硏究會誌 Vol.6 No.1

식물 생장조절제에 의한 녹두의 부위별 peroxidase, catalase 활성과 전기영동상 peroxidase 등위효소의 변화를 조사하였다. 녹두의 생육이 진행됨에 따라 하배측을 제외하고는 peroxidase 활성도는 증가하였으나 catalase 활성도는 감소하여 서로 상반된 결과를 나타내었다. 암발아시킨 녹두에 GA₃와 ABA를 각각 처리하면 peroxidase 활성도의 증가에 대하여 자엽에서만 GA₃가 ABA 보다 더 큰 영향을 끼쳤으나 catalase 활성도 변화는 ABA가 GA₃ 보다 더 큰 영향을 끼쳐 모든 부위에서 활성도를 증가시켰다. GA₃와 ABA를 혼합하여 처리하면 효소 활성도의 변화 경향은 GA₃보다 ABA에 더 큰 영향을 받았다. Peroxidase 등위효소의 수는 발아, 생육이 진행됨에 따라 모든 부위에서 계속 증가하여 활성도와는 무관하게 발아 후 6일째에 가장 많이 나타났으며, 호르몬의 처리에 따른 동위효소의 변화는 동위효소의 수에 대해서 보다는 intensity에 대하여 더 큰 영향을 받은 것으로 나타났다.

廢鑛山 周邊 土壤 및 植物體의 重金屬 存在形態와 含量調査

鄭相燮,朴昶東,姜相載,朴愚喆 慶北大學校農業科學技術硏究所 1993 慶北大農學誌 Vol.11 No.-

廢鑛山 周邊의 土壤 및 植物體에 存在하는 重金屬과 그들의 土壤에서의 存在形態에 따른 分布를 調査하였다. 土壤 中 重金屬 含量은 Mn을 除外하고 A土壤에서 제일 많았고, Mn은 B土壤에서 含量이 제일 높았다. 存在形態에 따른 각 重金屬의 含量은 각 元素別로 차이가 있었는데, 즉 Cd와 As는 有機態, Cu와 Pb 및 Mn은 Carbonate態, Fe와 Zn은 殘渣態가 다른 形態보다 더 높게 나타났다. 참억새와 골풀은 각각 75%와 15% 程度로 棲息하고 있었으며, 이는 이들 植物들이 重金屬에 대해 耐性을 가지고 있음을 보여주었다. 참억새와 골풀에 分布되어 있는 重金屬은 Mn을 제외하고 줄기보다는 뿌리에 더 많이 含有되어 있었다. 참억새의 경우 Zn은 줄기와 뿌리에 비슷하게 分布하는 것으로 나타났다. 周邊 用水의 分析 結果 浸出水에서 重金屬 含量이 높게 나타났으며, Fe와 Mn 및 Zn이 많이 檢出되었다. We investigated in the existential forms of heavy metals and their distribution in plant and soil surrounding ruined mine in Daduk area. The content of heavy metals in soils was more plentiful in A soil than that in another soil, excepting only Mn. The content of Mn in soils was greater in B soil than those in another soils. The content of each heavy metals, according to existential form, had different patterns on the comparision of each elements ; organic-form on Cd and As, Carbonate-form Cu, Pb and Mn and Residual-form on Fe and Zn. The popularity ration of M. senensis and J. decipiens were about 65% and 72%, respectively, and which means that these plants had resistances to heavy metals. The contents of heavy metals absorbed in root was higher than those of stem except Mn, In M. sinensis, the contents of Zn in root was similar to that of stem. To study the heavy metal contents in water, the highest content of heavy metal, mainly Fe, Mn and Zn, were calculated in extracting water.

優良室素固定菌의 探索分離 및 生理的 特性

朴愚喆,李光熙,金進鎬,李麟九,曺晋基 慶北大學校出版部 1987 慶北大農學誌 Vol.5 No.-

This experiment was conducted to search for Rhizobia with good nitrogen fixation abilities and to investigate their physiological characteristics isolated from 12 soybean cultivars and the affinities of root nodule bacteria with soybeans. The results obtained were as follows ; Based on colors, Rhizobia grown on YMA medium were divided into 3 groups, i. e., white, translucent and transparent, amounting to 60, 30 and 10%, respectively. In litmus milk reation, the strains which produced alkali, acid serum, alkaline serum and acid reached to 51, 29, 9 and 11%, respectively. Strains, S022, and S096 were slow-growers and produced alkaili, while strains, S080, S090, and S118 were fastgrowers and produced acid. The growth of root nodule bacteria on YMA medium was favorable between the initial pHs of 6.0~7.0. Glutamine, asparagine and allantoin as nitrogen sources enhanced the growth of root nodule bacteria. All the strains tested formed nodules on the soybean roots, and the strains with good symbiotic nitrogen fixation abilities that had white color, small colony, nitrate reduction abilities and no nitrite reduction abilities showed comparatively high nitrogen fixing activities. Some strains varied in nitrogen fixing activities according to soybean cultivars, and a few strains formed ineffective nodules which showed no nitrogen fixing activity.

승용차용 현가시스템 제어를 위한 반능동 ER댐퍼

김옥삼,박우철 여수대학교 1997 論文集 Vol.11 No.2

This paper presents a semi-active ER(electro-rheology) damper for vibration isolation of various types of vehicle. The Bingham properties of the ER fluids are first identified as a function of electric field and an appropriate size of the ER damper is manufactured on the basis of the level of the field-dependant yield shear stress. The damping forces of the ER damper is evaluated with respected to the intensity of the electric field as well as the number of operating cycle.

입력데이타 설계를 이용한 새로운 아이디 알고리즘

이현창,박우철 三陟大學校 2000 論文集 Vol.33 No.1

In system identification, the way one excites the system is crucial. For the most direct or indirect system identification methods, a random input signal is used. The randomness, in this case white gaussian and zero mean, should make sure that all unknown system modes are excited. The purpose of this work is to outline a input design such that the identification results are improved. In this paper, a new input design is proposed based on input/output data gathered from gathered from random excitation. The set of data is then used to compose a new set of input data, from which the system is excited and identified. The results obtained from numercal simulations show that the noise sensitivity is drastically reduced and accurate models can be identified, even with high noise disturbances.

Rhizobium japonicum에 있어서 變異株의 選拔 및 特性

朴昶東,姜相載,朴愚喆 慶北大學校農業科學技術硏究所 1994 慶北大農學誌 Vol.12 No.-

Rhizobium japonicum S118로부터 變異株를 選拔하고 生理的인 特性 및 植物體와의 相互關係등을 調査한 結果는 아래와 같다. N.T.G를 處理한 S118로부터 根瘤를 形成하지 않는 SM255, 根瘤着生이 아주 좋고 acetylene還元力도 높은 HP277, 根瘤着生이 S118보다 떨어진 LP268 그리고 根瘤가 아주 드물게 形成되는 SM303을 選別하였다. SM255를 接種한 植物體는 生育이 低調하였고, 뿌리털의 curling 現象도 觀察되지 않았다. 반면 HP277은 SM255와 서로 다른 結果를 나타내었다. Litmus milk 反應에서 HP277이 alkali性, serume zone을 形成하였고, congo red를 弱하게 吸收하였다. YEM培地에서 SM255는 生育이 S118과 비슷한 slow-growing型이지만 HP277은 fast-growing型이었다. 窒酸 및 亞窒酸 還元은 LP268이 陰性, 陰性, SM255는 陽性, 陽性, 그外 菌株는 陽性, 陰性으로 나타났다. SM255는 無機態와 有機態 窒素源의 影響을 받아 生育이 低調하였다. 그러나 HP277은 Ca(NO₃)₂·4H₂O의 10mM, 25mM濃度에서 生育이 가능하였고, 有機態 窒素源의 利用이 높은 것으로 나타났다. 모든 菌株가 starch를 利用하지 못하였고 arabinose의 경우 SM255와 SM303은 他菌株에 비해 利用性이 낮음을 알 수 있었다. 그러나 sucrose는 모든 菌株가 利用할 수 있었다. HP277과 S118은 菌體 蛋白質의 組成에 差異가 없었고, SM255는 Rm 0.62의 band가 缺如되어 있었다. This experiment was conducted to isolate the mutants from S118 and to investigate the physiological characteristics of R. japonicum mutants. The results obtained were as follows; Based on nodulation and acetylene reduction, nodulation of rhizobia was divided into 4 groups, i.e. slow-nodulation, earlier-nodulation, infrequent-nodulation and non-nodulation. At 5% significant level, the growth of inoculated plant with SM255 was bad, but that of HP277 was good. Root-hairs curling was induced by strains S118 and HP277 on soybean, but not by strain SM255. S118 and SM255 were found to be slow-gorwers and produced alkali, whereas stratin HP277 was fast-grower and produced acid in YEM broth. In litmus milk reaction, all strains indicated alkaline reaction, and serume-zone was induced weakly by HP277. All of the strains tested in this experiment utilized sucrose. HP277 and LP268 utilized xylose, whereas S118 and SM255 did not. SM255 showed bad growth in nitrogen carriers however utilization of Ca(NO₃)₂·4H₂O by HP277 was possible at 25mM and 10mM level. To compare with S118, the protein band of SM255's cell protein electrophoresis was not developed at 0.62 Rm position.

기획연재 / 한국 사회주의 운동의 미래 2 : ' 민주주의와 사회주의의 문제 , 이병천류의 청산주의와 「 현실과 과학 」 류의 교조주의를 비판한다 '

박우철 사회평론 1992 월간 사회평론 Vol.92 No.1

Some Properties of Partially Purified Alkaline Inorganic Pyrophosphatase from Mung-bean (Phaseolus radiatus) Seedling

박우철,남민희,Park, Woo-Churl,Nam, Min-Hee 한국토양비료학회 1982 한국토양비료학회지 Vol.15 No.4

발아초기 녹두의 부위별 alkaline inorganic PPase의 활성변화 및 잎 부위에서 부분 정제하여 얻은 효소를 이용하여 효소적 성질을 조사한 결과를 요약하면 다음과 같다. 1. 잎 부위가 타 부위보다 약 2~4배의 더 높은 활성을 나타냈으며 발아가 진행됨에 따라 잎과 뿌리 그리고 지엽에서는 효소 활성이 초기에 증가하다가 점차 감소하는 경향을 보인 반면, 상배축에서는 초기부터 계속 감소하였으며 하배축에서는 계속 증가하는 경향을 보였다. 2. 정제 과정동안 23.9%의 수율로 86배가 정제되었으며 전기 영동상의 Rm value는 0.35였으며 homogenenity는 아니었고, Km value는 0.89mM로 나타났다. 3. 이 효소는 $Mg^{2+}$에 대해 대단히 Specific하였으며 $Cu^{2+}$와 $Fe^{2+}$도 $Mg^{2+}$에 비해 각각 56%, 55%의 activating effect를 나타냈다. 그러나, $Cu^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$와 $Ni^{2+}$은 이 효소에 대해 저해체로 작용하였다. 4. 이 효소는 pH 8-9와 $50^{\circ}C$에서 최대의 활성을 보였으며 열에 대해서도 상당히 안정하였다. Time course of alkaline inorganic PPase activity with different parts of mung-bean sprout and some properties of partially purified enzyme from mung-bean leaves were investigated. The enzyme activity in leaf, root and cotyledon showed a tendency to increase at an early stage and then decrease gradually as germination continued. However, the crude homogenate of epicotyl showed the continuous decline of the enzyme activity but that of hypocotyl showed the continuous increase. In particular, the enzyme activity of leaf fraction was about 2-4 times as high as those of other fractions. The specific activity of the leaf enzyme was increased 86-fold, with a 23.9% yield, upon purification procedures. The purified enzyme from leaves had the Rm value of 0.35 and was not homogenous when judged by disc gel electrophoresis. Using tetrasodium pyrophosphate as a substrate, the apparent Km value for the partially purified enzyme was 0.89 mM. The enzyme was highly specific for $Mg^{2+}$ $CU^{2+}$ and $Fe^{2+}$ was also showed the activating effect of 56% and 55% with $Mg^{2+}$, respectively. However, $Ca^{2+}$), $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$ and $Ni^{2+}$ acted as inhibitors for the enzyme. The pH optimum for the enzyme shifted from 9.0 to 8.0 as the concentration of $Mg^{2+}$ was increased. The enzyme from mun-bean leaf was the most active at $50^{\circ}C$ and considerably stable on heat.

學校單位 現場敎育硏究 基盤造成

박우철 인간교육연구원 1994 人間敎育硏究 Vol.1 No.-

전기유동유체의 전기적 내구성에 대한 실험적 고찰

박우철,이현창,이형식 三陟大學校 2001 論文集 Vol.34 No.1

This paper presents experimental results on material properties of an electro-rheological(ER) fluids subjected to electrical fatigues. As a first step, two types of ER fluids made of arabic gum and methy1 cellulose(MC) choosing 25% of particle weight-concentration. Following the construction of test mechanism for electrical durability of ER fluid, the dynamic yield shear stress and current density of the ER fluids are experimentally distilled as a function of electric field. The yield shear stress of operated ER fluids are distilled and compared with those of unused ER fluids. In addition, the surface roughness of the employed electrode are evaluated as a function of the number of the electric-field cycle.