Streptococcus salivarius의 요소분해효소 활성에 관한 연구

정상백,최호영,민병순,박상진,이진용,최기운 大韓齒科保存學會 1998 Restorative Dentistry & Endodontics Vol.23 No.1

Dental caries is induced by organic acids produced by oral bacteria. In order to prevent dental caries, therefore, it is essential to maintain neutral pH in the oral cavity. Urea plays a major role in oral pH homeostasis. Urea is hydrolyzed by bacterial ureases to ammonia, causing a pH elevation, Streptococcus salivarius has been shown to be a major contribution to oral ureolysis. Synthesis of urease by S. salivarius appears to be constituitive, but can be greatly enhanced by low pH. It is, therefore, conceivable that ureolytic activity of S. salivarius from a carious lesion is greater than that of the bacterium from a healthy tooth. In the present study, urease activity of S. salivarius isolates from dental plaque of carious lesions was compared with that of the isolates from plaques of the teeth and the dorsum of the tongue; 45 S. salivarius strains were isolated from carious lesions(>C2) of 21 individuals with dental caries and 30 strains from 10 individuals without dental caries. The results were as follows: 1. All the 21 individuals with dental caries harbored ureolytic S. salivarius whereas 3 of 13 individuals without dental caries harbored non-ureolytic strains of S. salivarius. 2. All the 45 S. salivarius isolates from carious lesions showed urease activity. In contrast, of 30 isolates from individuals without dental caries, 17 isolates(56.7%) did not demonstrate urease activity, or if any, very little(<5μmol/min/mg). 3. Urease activity of the isolates from carious lesions was greater than that of the isolates from individuals without dental caries:the urease activity ranged from 42 to 381μmol/min/mg and from 0 to 208μmol/min/mg, respectively. 4. At acid pH(5.5), the isolates which showed intermediate urease activity at pH 7.0 demonstrated even higher activity whereas the isolate with no or lower urease activity did not show any significant difference in their activity. However, the isolates with the greatest urease activity from both individuals with and without dental caries, exhibited a rather much lower urease activity at pH 5.5. The overall results suggest that isolates may have their own urease activity but the isolates exposed to chronic acidic environment of the carious lesion might elevate urease activity of S. salivarius, which in turn, might influence on survival of S. salivarius itself and other bacteria establishing a new oral bacterial ecosystem.

주파수 선택성 레일리 페이딩 환경에서 부호화 기법을 이용한 직교 멀티캐리어 시스템의 오율 해석

정재필,박기식,한성운,조성준 한국항공대학교 1997 論文集 Vol.35 No.-

본 논문에서는 주파수 선택성 레일리 페이딩 환경에서 RS부호화, 컨벌루션 부호화, 연접부화를 이용하는 직교 멀티캐리어 BDPSK시스템과 QDPSK시스템의 오율특성을 구하였다. 오율특성은 정규화된 지연시간(??), 비트에너지 대 잡음전력 스펙트럼 밀도비(??), 희망 신호전력 대 지연되 비희망 신호전력비(D/U)를 파라미터로 하여 나타내었다. 검토한 결과, BDPSK 신호인 경우 D/U가 20[dB]일 대 오율을 ??으로 유지하기 위해 필요한 Eb/No는 Rs부호화, 컨벌루션 부호화 및 연접부호화에서느 각각 대략 20.8[dB], 16.0[dB], 14.5 [dB]가 필요하며, QDPSK신호인 경우 Rs부호화, 컨벌루션 부호화 및 연접부호화에서 각각 대략 24.0[dB], 18.9[dB], 13.5[dB]가 필요함을 알 수 있었다. This paper has evaluated the error performance of orthogonal multicarrier BDPSK and QDPSK systems which use Reed Solomon coding, convolutional coding, and concatenated coding technique in a frequency selective Rayleigh fading environment. The results show that when D/U (desired wave's power to undesired delayed wave's power ratio)=20dB at error rate of ??, Reed Solomeon coded BDPSK signal, convolutional coded BDPSK signal, and concatenated coded BDPSK signal need about 20.8[dB], 16.0[dB], 14.5[dB] respectively in terms of Eb/No, and Reed Solomon coded QDPSK signal, convolutional coded QDPSK signal, and concatenated coded QDPSK signal need about 24.0[dB], 18.9[dB], 13.5[dB] respectively.

패키지디자인에 나타난 B.I차별화 전략연구 : 샴푸 개발사례를 중심으로

정운성 한국패키지디자인학회 2002 상품문화디자인학연구 Vol.11 No.-

Shampoo is one of important products to remove pollutants existing on hair and skin in our body, to maintain them sanitary, to make ourselves up. it has been classified such as perfume, 2in1, mild, treatm ent, and dandruff prevention according to consumer is trend varieties, out of the meaning just to cleanse They also have been changing about the kind of cosmetic Personal Care products that personal inclination leads. The transition for the needs by changing consumers propensity to consume is the natural result followed by the improvement of consumers income, education, and culture level. The domestic shampoo market is expected to grow, as it entered its maturity level in the 1990s as a turning point, to expand in quality rather than in quantity With the growth of highprice market from 1998, 20 is female consumers increase is leading global brand such as P&G and Unilever to grow up, meanwhile, domestic brands such as LG Household & Health Care, Pacific, and Aeakyung to get weakened in terms of competitiveness. This article is to suggest that the proper measurement among several to have strong brand competition is to strengthen the differentiation strategy of Brand Identity in terms of package design. The effective differentiation strategy of B.I means a systematic schematization in the business strategy level. B.I for local consumers and the realities of B.I application by domestic local brands that being traded in the domestic area is far beyond to the This article analyzes the existing state of B.I differentiations of products, which compete. This article analyzes the existing state of B.I differentiations of products, which competeaddition, this is to survey what has more competition and distinction base on B.I differentiation, and to propose the direction with the hope of elevating the competition level of local brands further.

CuInS_2 박막 제조 및 그 특성

정해덕,박계춘,조재철,정운조,김성구 木浦大學校 工業技術硏究所 1992 工業技術硏究誌 Vol.2 No.-

The polycrystalline CuInS_2 thin films are prepared by vacuum heat treatment of layer, which is deposited by vaccum evaporation in order. The electrical and optical properties of the films are investigated at various sulfur deposition mole rate, substrate temperature, heat treatment temperature and time. From data, n type- CuInS_2 exhibits resistivity, transmittance and energy band gap with 142[Ωㆍ㎝], 73[%], and 1.5[eV] respectively at optimum fabrication condition. Finally, the films are fabricated with chalcopyrite structure.

영역분할과 투영기법을 이용한 내용기반 영상검색

金廷炫,金商雲 명지대학교 산업기술연구소 2005 産業技術硏究所論文集 Vol.24 No.-

This paper proposes a content based image retrieval system using a segmentation technique, where we employ the Watershed Segmentation and Horizontal/Vertical Projection Profile schemes to extract shape features. The proposed method has been tested and compared with a conventional retrieval method on both "animal pictures" and "character" image sets. Our experimental results demonstrate that the proposed mechanism retrieve the query image efficiently. The results especially demonstrate that the proposed method can be used to efficiently retrive animal objects from an image database.

CO₂고정을 위한 화학독립영양미생물 Aeromonas sp. strain JS-1의 분리 및 특성

배상옥,곽경오,이용운,김성준,정선용 전남대학교 촉매연구소 2000 觸媒硏究 論文集 Vol.21 No.-

The increase of CO2 in the atmosphere by fossil-fuel combustion has been recognized as a major cause of global warming. Thus many studies on CO2 removal from the combustion gas have been performed, and recently the techniques for biological CO2 fixation by photoautotrophs have also been developed, In this study, extensive screening was conducted to obtain micoorganism with high capability in fixing CO2, and its characteristics was investigated. As a result, a chemoautotrophs identified as a Aeromonas sp. strain JS-1 was isolated from the fresh water. The isolated strain used the molecular hydrogen and CO2 as an energy and a carbon source, respectively. Growth characteristics, which will be used in the development of a continuous culture system related in CO2 fixation, were examined in batch cultivation under conditions of various CO2 and NaCl concentrations, temperature, and pH. The results of the examination showed that the isolated strain had a good growth to high CO2 concentration(40%), indicating applicable in removing CO2 discharged from industries.

갑상선결절의 임상 및 초음차소견의 진단적 가치

박거운,박철진,박일구,신지혜,정중화,배학연,김상용 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Backgrounds: Thyroid nodule have been relatively common disease and it's prevalence estimated about 4-7%. Recently, high resolution ultrasonography has made the detection of small thyroid nodule possible, Increases in the detection of thyroid nodule have created a clinical dilemma on how to properly manage such thyroid nodules. We investigated the prevalence, clinical and ultrasonographic characterristics, and optimal diagnostic approach toward detected benign and malignant thyroid nodules. Methods: A retrosepctive review was undertaken on the 372 patients who was done Ultrasonograpy guided fine needle aspiration biopsy in Chosun University Hospital, Gwang-Ju, Korea between July 2003 and Spring 2005. Devided into four group such as benign and malignant thyroid nodule on the basis of size 1.5Cm. The review consisted of thyroid function test, antithyroid antibodies, thyroid autoantibodies, thyroid ultrasonography, fine needle aspiration biopsy in each group. Results: The prevalence of thyroid nodule malignancy rate was 18.0%. The prevalence of thyroid incidentaloma malignanct rate was 17.5%. There were no significant differences in age, sex, thyroid function test and size between the benign, malignant incidentalomas and nodule. Ultrasonographic characteristics, include ultrasonography index point showed meaningful diagnostic value for the detection of malignancy in thyroid nodule, Conclusion: Thyroid cancers are fairly common finding. There are no clinical difference between benign and malignant thyroid nodule; however, ultrasonographic findings can be used to decision of optimal management strategies 배경: 최근갑상선 초음파의 빈번한 시행으로 갑상선 결절의 발견이 증가하고 있다. 이러한 갑상선 결절중 특히 갑상션우연종을 임상적으로 어떻게 접근하고 치료를해야하는지는 적지 않는 문제가 되고 있다. 본 연구에서는 갑상선 우연종 및 직경 1.5 cm 이상의 갑상션결절의 유병률, 임상적 특징, 초음파특정 및 초음파의 진단적 유용성에 대해 알아 보았다. 방법: 2003년 7윌부터 2005년 3월까지 조선 대학교병원 내과 및 일반외과및 건강검진 센터를 통해 갑상선 초음파, 경동맥 초음파 및 경부 CT등을 통해 우연히 발견된 갑상션결절을 가진 372명을 대상으로 후향적 연구를 시행하였다. 크기 1.5 cm 미만의 양성및 악성 갑상선 결절군과 크기 1.5 cm 이상의 양성 및 악성결절로 그룹을 나누고 각 군에서 나이, 성별, 갑상선 기능검사, 항갑상선 항체, 갑상선 초음파 및 미세침흡인세포검사등을 검토하였고, 모든 갑상선 결절에 Koike (6)가 제한한 초음파지표 점수(Ultrasonographie index point)를 부여하여 각 군을 비교하였다. 결과: 분석한 갑상선 결절 372예 중 악성결절의 유병율은 18.0% (67예)였다. 갑상선 우연 종의 유병율은 81.5% (303예)였다. 갑상선 우연 종에서의 악성률은 17.5% (53예)로 나왔다. 크기 1.5 cm 이상의 양성 및 악성 갑상선 결절과 양성 및 악성 갑상선 우연종에서 나이, 성별, 갑상선 기능검사, 항갑상선항체에는 의미있는 차이가 없었다. 또한 양성 및 악성우연종에서 의 갑상선 결절크기에도 의미있는 차이는 보이지 않았다. 갑상선 결절의 초음파 지표점수만이 양성 및 악성우연종과 양성 및 악성갑상선 결절의 감별에 의미있는 차이를 보였다. 갑상 선우연종과 크기 1.5 cm 이상의 갑상선 결절에 서 양성을 나타내는 초음파지표점수는 각각 3 점과 4점으로 다르게 나타났다. 양성 및 악성 갑상선우연종의 감별에서 초음파 지표점수의 민감도는 93.6%, 특이도는 52.8%, 양성예측율은 92.4%로 나왔고, 진단적 효율은 86.4% 였으며 크기 1.5 cm 이상의 양성 및 악성 갑상선 결정의 감별에서 초음파지수 민감도는 92.7%, 특이도가 42.9%, 양성 예측도 86%, 효율 82.6%를 보였다. 결론: 갑상선결절에서의 양성 및 악성의 유무는 임상적으로 판단하기 힘들지만 갑상선 결절에서의 초음파 소견 및 초음파 지표 점수를 도입하면 이에 대한 감별에 도움을 줄 수 있다. 따라서 갑상선 초음파검사시 초음파지표점수의 사용은 향후 갑상선 결절의 진단 방향을 제시하는 중요한 길잡이가 될 수 있으리라 사료된다.

Anisakis type I 幼蟲에 의한 人體感染 2例

손운목,고원규,설상영,정정명 인제대학교 1991 仁濟醫學 Vol.12 No.3

91년 5월 17일에 上腹部痛을 主訴로 本院을 내원한 2명의 患者에 대하여 胃 內視鏡檢査를 시행하였던 바, 胃 粘膜에 길이로 묻혀 있는 Anisakis 幼蟲을 1마리씩 檢出하였다. 胃 粘膜이 충체를 중심으로 肥厚되어 있었고 肉芽腫性 病巢가 형성되어 있었다. 검출된 충체는 둘다 Anisakis type I 幼蟲이었다. Two cases of gastric anisakiasis caused by Anisakis type I larva were reported. The patients, residing in Pusan, were 66-year-old male and 36-year-old female. They chiefly complained of epigastric pain and recalled they had eaten raw marine fish such as sea eel and bream. Two whitish round worms were found in the hypertrophic and granulomatous mucosa of stomach wall on gastroscopic examinations, and were classified as Anisakis type I larva.

茵蔯淸肝場의 B型 및 C型 간염 바이러스에 대한 抗 바이러스 效果

김수성,김기열,윤철호,서운교,김종대,정지천 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.8 No.1

우리나라와 같이 B형 간염 바이러스의 유병율이 높은 지역에서는 B형 간염 바이러스 뿐만 아니라 HCV에 대한 항 바이러스 효과도 동시에 시행함이 유용할 것으로 사료되었다. 본 연구에서는 茵蔯淸肝湯을 이용하여 B형 간염 바이러스에 대한 抗 바이러스 효과를 PCR을 이용하여 HBV의 DNA를 검색하였다. 茵蔯淸肝湯이 처리된 처리군의 경우 대조군에 비하여 농도 의존적으로 B형 간염 바이러스의 표면항원 생산을 억제하였고, PCR을 이용하여 증폭한 경우에도 증폭된 DNA의 양은 감소하였다. 또한 C형 간염 바이러스에 대한 抗 바이러스효과를 검색하기 위하여 복제에 필수적인 단백질인 HCV helicase의 ATPase에 대한 활성 억제 효과를 조사하였다. 茵蔯淸肝湯이 HCV helicase의 ATPase 활성을 억제하는 효과가 있음을 확인한 후 dose-respond에 따른 실험을 수행하여 HCV helicase의 ATPase 활성을 억제함으로써 탈인산화 되지 않은 ATP의 양을 이용하여 PSL로 분석한 결과 茵蔯淸肝湯이 HCV helicase의 ATPase 기능에 대한 억제효과가 우수함이 확인되었다. 이상의 결과로 보아 茵蔯淸肝湯이 B형 및 C형 간염 치료에 동시에 응용될 수 있을것으로 사료된다. For the purpose of investigate the inhibition effects of Injinchunggantang on Hepatitis and C Virus. The inhibition experiments were as follows : 1. The inhibition effects of Hepatitis B surface antigen production was remarkably increased in the pretreated group of Injinchunggantang compared with control group. 2. Amplified PCR products of HBV-DNA in culture media was considerably decreased in the pretreated group of Injinchunggantang compared with control group. 3. Results of ATP quantitation and ATPase inhibition percent was increased pretreated group of Injinchunggantang. Also ATPase activity of HCV helicase inhibition assay was increased with a dose dependent manner. These results may suggest that Injinchunggantang will have the inhibition effects of Hepatitis B and C Virus.

교량의 실시간 거동 모니터링을 위한 GPS의 적용

박운용,정성모,곽두호,이흥규 東亞大學校 大學院 1999 大學院論文集 Vol.24 No.-

Real Time Kinematic GPS will allow a multitude of monitoring, navigation and engineering applications. Applications inclued real time deformation and movement monitoring and real time automated plant control. In a low dynamic environment, with a short baseline length, it is possible to position a roving receiver to an approximate accuracy of less than 5mm within a few seconds of thestart of the ambiguity. One Engineering application is the monitoring of the movement of cable suspension bridges. Such structures are capable of movements of up to a few meters under adverse wind conditions or heavy traffic loads. The use of relative OTF positioing at strategic points upon the bridge would allow close monitoring of the bridge's movements and calculate deformation and long-term deterioration factors for the bridge. Future bridge designs and traffic management schemes could also benefit from the monitoring system.