Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

압밀배수공법에 따른 압밀효과 비교 연구

정진호,김근수 釜慶大學校 1999 釜慶大學校 論文集 Vol.4 No.1

The feed-back analysis based both on the in-situ measured values of the settlement of the embankment and on the soil properties of soft clay under the embankment is carried out in this thesis for the Preloading method and the Vertical drains, such as the Pack drains and the P.V.D.. The feed-back analysis is performed with the TCON program which has generally been used to estimate the average degree of consolidation and the final settlement of soft clay under the surcharge. From the results of the feed-back analysis for the final settlement, we can find that there are no significant differences in the behavior of consolidation between the Pack drains and the P.V.D. - the Pack drains show 73.6~75.8% of the average degree of consolidation, the P.V.D., 68~75% and the Preloading method, 48.5~58.7%. Asaoka, Hyperbolic and Hoshino method are also used to predict the final settlement of the embankment ofr the Vertical drains and the Preloading method. The final settlement estimated by the Hyperbolic method appears to be the most close to that by the feed- back analysis.

은행의 부실채권 처리 방안에 관한 연구

정수원,박태진 東西大學校 1998 동서논문집 Vol.4 No.-

The purpose of this paper is to examinate of the Financial Structure Reform in Korea and Japan. Through examination of the Financial Structure Reform in Japan, the suggestions to Korea is as follows. 1. To securitization of insolvency assets is better than injection of government fiscal funds. 2. We have to establish rule of the Financial Structure Reform. 3. We have to stop "too big to fail" and have to readjust insolvency big banks. 4. We have to strengthen of supervisory function on an enterprise by banks.

국내 아파트 가변형 시스템 적용에 관한 연구

정진원,박진수 경기대학교 산업기술종합연구소 2006 산업기술종합연구소 논문집 Vol.31 No.-

상악 전치부 후방 견인 시 이동 양상과 응력 분포에 관한 삼차원 유한요소법적 연구

정애진,김운수,이수행,강성수,최희인,조진형,김상철 대한치과교정학회 2007 대한치과교정학회지 Vol.37 No.2

상악 전치부 후방 견인 시의 이동 양상 및 응력 분포를 건조 두개골을 전산화 단층 촬영에 의해 3차원 영상화한 유한 요소 모델 상에서 알아보고자 하였다. 피질골 절단술의 시행 여부와 고정원, 힘의 작용점을 각기 달리 설정하여 8개의 실험군을 구성하여 비교하였다. 통상적인 T-loop을 이용하여 공간폐쇄를 하는 경우 전치부는 후하방으로 경사이동 하였으며, 구치부에서도 약간 전방 이동하였다. 피질골 절단술을 동반하여 전치부를 견인한 경우, 전치부 골편에서의 응력 분포가 전반적으로 넓게 분포되었으며, 전치부 경사의 정도가 적은 반면에 변위량은 훨씬 더 많았다. 협측에서의 견인 시 상악 협측에 식립된 미니 임플랜트와 견치 power arm간에 견인력을 가한 경우가 미니 임플랜트로 고정원이 강화된 제2소구치와 견치 브라켓 간에 견인력을 가한 경우보다 전치부의 후하방 경사 정도가 적었다. 구개측에서 power arm에 대한 견인 시 정중구개봉합 부위에 식립한 미니 임플랜트로부터 견인력을 가한 경우가 상악 제1, 2대구치 간 구개면에 식립된 미니 임플랜트로부터 견인력을 가한 경우보다 전치부의 후하방 경사 정도가 컸다. 이러한 결과로써 치아이동 시 피질골 절단술의 효과와 저항중심에 대한 교정력 벡터 조정의 의미를 확인할 수 있었다. Objective: The purpose of this study was to evaluate the displacement pattern and the stress distribution shown on a finite element model 3-D visualization of a dry human skull using CT during the retraction of upper anterior teeth. Methods: Experimental groups were differentiated into 8 groups according to corticotomy, anchorage (buccal: mini implant between the maxillary second premolar and first molar and second premolar reinforced with a mini implant, palatal: mini implant between the maxillary first molar and second molar and mini implant on the midpalatal suture) and force application point (use of a power arm or not). Results: In cases where anterior teeth were retracted by a conventional T-loop arch wire, the anterior teeth tipped more postero-inferiorly and the posterior teeth moved slightly in a mesial direction. In cases where anterior teeth were retracted with corticotomy, the stress at the anterior bone segment was distributed widely and showed a smaller degree of tipping movement of the anterior teeth, but with a greater amount of displacement. In cases where anterior teeth were retracted from the buccal side with force applied to the mini implant placed between the maxillary second premolar and the first molar to the canine power arm, it showed that a smaller degree of tipping movement was generated than when force was applied to the second premolar reinforced with a mini implant from the canine bracket. In cases where anterior teeth were retracted from the palatal side with force applied to the mini implant on the midpalatal suture, it resulted in a greater degree of tipping movement than when force was applied to the mini implant between the maxillary first and second molars. Conclusion: The results of this study verifies the effects of corticotomies and the effects of controlling orthodontic force vectors during tooth movement.

소아기 외과계질환의 통계적 관찰

정용헌,이재호,이건수,서종진 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

Child is not little man but rather a father of man. Children are faced to surgical and medical problems reqiring various specialists. Authors analized 1126 pediatric surgical in-patients of Chung-nam National Univ. Hospital from Jan. to Dec. 1988 by departments, age, sex, duration of admission and types of diseases in order to get the information for management of children’s hospital. The results are as follows: 1. Absolute numbers of in-patients by departments are above 200 in general surgery and opthalmology, between 100-199 in plastic surgery, ENT, chest surgery and below 99 in urology and neurosurgery. 2. Male to female ratio as a whole is 1.8:1, male dominant. 3. Duration of admission within 1 week was of 55% as a whole, 87% in ENT, 85% in ophthalmology, 13% in orthopedic surgery. 4. Average duration of admission was 10.8 days as a whole, 4.6 days in ENT, 5.1 in ophthalmology, 29.7 days in orthopedic surgery, and variable by types of diseases. 5. The most frequent disease by departments was hernia in general surgery, fracture in orthopedic surgery, cerebral contusion in neurosurgery, ventricular septal defect in chest surgery, strabismus in ophthalmology, tonsillitis & adenoiditis in ENT.

사람, 집박쥐 및 마우스 파네트세포의 Lysozyme 면역반응성

정진웅,차정호,오수자,전정수 대한해부학회 1991 Anatomy & Cell Biology Vol.24 No.2

전류제어형 PWM컨버터를 이용한 동기발전기용 여자시스템에 관한 연구

장수진,류동균,서민성,김준호,원충연,이진국 성균관대학교 2003 학술회의지원논문목록집 Vol.2003 No.-

동기발전기 출력전압은 여자시스템의 계자전류 제어에 의해 일정하게 유지된다. 고주파 PWM 컨버터(전류제어모드 buck컨버더)형태의 여자시스템은 부하변동이 발생하였을 때 동기발전기의 계자전류를 제어하게 된다. 이 논문은 정상상태 및 과도상태에서의 안정화를 개선하기 위하여 여자시스템의 설계 및 실험에 대해 다루었다. 시뮬레이션 및 실험 결과는 제안된 여자시스템이 50kW 동기발전기의 DVR에 의해 응답시간이 개선되었음을 보여주었다. The output voltage of synchronous generator is regulated constantly by field current control in excitation system. High frequency PWM converter (current control mode buck conveter) type excitation system for synchronous generator is able to control exciter current when the load change happened. This paper deals with the design and evaluation of the excitation system for a synchronous generator to improve the steady state and transient stability. The simulation and experimental results show that the proposed excitation system is able to improve the response time by the DVR(digital voltage regulator) of 5O[kW] synchronous generator.

복합식 히트펌프 설계를 위한 액체건조제 제습기의 성능 특성에 관한 실험적 연구

이수동,정진은,박문수 한국기술교육대학교 2004 論文集 Vol.10 No.2

A liquid desiccant for hybrid heat pump system undergoes various influences by several working factor. This paper presents the study of six options in related with the dehumidification rate and efficiency absorption in the dehumidification system. The effect of operating variables such as inlet solution concentration, inlet solution flow rate, inlet solution temperature, inlet air flow rate, inlet air temperature, and brine temperature was analyzed. The experimental dehumidifier has been designed to study the dehumidi- fication characteristics of the aqueous triethylene glycol(TEG) system. The performance characteristics of the absorption dehumidification system can be used effectively to save energy by reducing latent heat load. The result of this experiment can provide useful data for hybrid air conditioning system.

광주지역 소아에서 유행한 호흡기바이러스 유행양상 분석

서진종,김민지,김선희,기혜영,정재근,김은선,박종태,김경심,이수야,김명권,정윤석 대한감염학회 2008 감염과 화학요법 Vol.40 No.4

Background : This investigation was to perform the epidemiological surveillance and genetic analysis on respiratory viral agents from children with acute respiratory infections in Gwangju. Materials and Methods : For this study, 3,695 specimens obtained from patients with acute respiratory infections were collected by collaboration with pediatric hospitals in Gwangju between 2005 and 2007. Specimens were screened for 8 respiratory viruses including influenza viruses (IFV), human rhinoviruses (HRV), human coronaviruses (HCoV), adenoviruses (ADV), parainfluenza viruses (PIV), human enteroviruses (HEV), respiratory synthitial viruses (RSV) and human bocaviruses (HBoV). Respiratory viruses were detected using multiplex (RT) PCR with viral specific primers. Results : Out of 3,695 specimens, the ratio of virus detection was 24.9% (919). Overall, HRV (35.5%) and IFV (34.9%) were the most common viruses found, followed by HBoV (14.8%), HCoV (10.6%), RSV (3.7%), ADV (3.4%), PIV (3.2%) and HEV (3.0%). In addition, multiple infections were detected in 80 patients (8.7%). When the prevalence was analyzed according to season, HBoV, IFV and HCoV showed two epidemic points in late spring and early winter. ADV, HRV, RSV PIV and HEV, however, were all found to have only one epidemic point, with RSV being most common during winter and the others being most prominent during spring. Conclusions : Through this epidemiological surveillance, the respiratory viruses prevalent in children in Gwanaju area were investigated. We strongly recommend the development of nationwide policy for the management of prevalent respiratory virus that includes long term collection of data and samples, vaccine development and prevention education of the misuse of antibiotics.