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Effect of By-Product Gypsum Fertilizer on Methane Gas Emissions and Rice Productivity in Paddy Field
Jun-Hong Park,Yeon-Kyu Sonn,Myung-Suk Kong,Yong-Seon Zhang,Sang-Jo Park,Jong-Gun Won,Suk-Hee Lee,Dong-Hwan Seo,So-Deuk Park,Jang-Eok Kim 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.1
Rice cultivation in paddy field affects the global balance of methane (CH₄) as a key greenhouse gas. To evaluate a potential use of by-product gypsum fertilizer (BGF) in reducing CH₄ emission from paddy soil, CH₄ fluxes from a paddy soil applied with BGF different levels (0, 2, 4 and 8 Mg ha<SUP>-1</SUP>) were investigated by closed-chamber method during rice cultivation period. CH₄ flux significantly decreased (p<0.05) with increasing level of BGF application. 8 Mg ha<SUP>-1</SUP> of BGF addition in soil reduced CH₄ flux by 60.6% compared to control. Decreased soil redox potential (Eh) resulted in increasing CH₄ emission through a CO₂ reduction reaction. The concentrations of dissolved calcium (Ca) and sulfate ion (SO₄<SUP>2-</SUP>) in soil pore water were significantly increased as the application rate of BGF increased and showed negatively correlations with CH₄ flux. Decreased CH₄ flux with BGF application implied that SO₄<SUP>2-</SUP> ion led to decreases in electron availability for methanogen and precipitation reaction of Ca ion with inorganic carbon including carbonate and bicarbonate as a source of CH₄ formation under anoxic condition. BGF application also increased rice grain yield by 16% at 8 Mg ha<SUP>-1</SUP> of BGF addition. Therefore, our results suggest that BGF application can be a good soil management practice to reduce CH₄ emission from paddy soil and to increase rice yield.
입원 중인 베트남 참전 군인의 삶의 만족도와 관련된 요인
김애리,박종,김율,노희송,박광희,김은숙,김형철,홍강식,류소연,강명근,김기순 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2
Background and Objectives: The aim of the study was to identify the factors associated with life satisfaction of hospitalized Vietnam veterans. Material and Methods: The data were collected from 200 hospitalized Vietnam veterans of 5 veterans hospitals in Korea from September 13 to 23, 2004. Information of general characteristics, health behavior, disease history, family function, depression and life satisfaction were collected by structured questionnaire. Results: The mean of life satisfaction was 21.48(±6.03), and the mean of depression was 53.10(±9.64). The prevalence of depression was mild depression 24.0%, moderate depression 43.5% and severe depression 25.0%. Depression was associated with life satisfaction and depression was higher, life satisfaction was lower. Family function and age were positively associated with life satisfaction, Conclusion: Life satisfaction was affected by depression and family function in hospitalized Vietnam veterans. Therefore, it was thought that psychosocial approaches such as improve depression and family function in care of hospitalized Vietnam veterans is required.
Generation of red fluorescent protein transgenic dogs
Hong, So Gun,Kim, Min Kyu,Jang, Goo,Oh, Hyun Ju,Park, Jung Eun,Kang, Jung Taek,Koo, Ok Jae,Kim, Teoan,Kwon, Mo Sun,Koo, Bon Chul,Ra, Jeong Chan,Kim, Dae Yong,Ko, CheMyong,Lee, Byeong Chun Wiley Subscription Services, Inc., A Wiley Company 2009 Genesis Vol.47 No.5
<P>Dogs (Canis familiaris) share many common genetic diseases with humans and development of disease models using a transgenic approach has long been awaited. However, due to the technical difficulty in obtaining fertilizable eggs and the unavailability of embryonic stem cells, no transgenic dog has been generated. Canine fetal fibroblasts were stably transfected with a red fluorescent protein (RFP) gene-expressing construct using retrovirus gene delivery method. Somatic cell nuclear transfer was then employed to replace the nucleus of an oocyte with the nucleus of the RFP-fibroblasts. Using this approach, we produced the first generation of transgenic dogs with four female and two male expressing RFP. genesis 47:314–322, 2009. © 2009 Wiley-Liss, Inc.</P>
Post-mortem re-cloning of a transgenic red fluorescent protein dog
So Gun Hong,구옥재,Hyun Ju Oh,박정은,김민정,김지은,Eun Jung Park,장구,이병천 대한수의학회 2011 JOURNAL OF VETERINARY SCIENCE Vol.12 No.4
Recently, the world’s first transgenic dogs were produced by somatic cell nuclear transfer. However, cellular senescence is a major limiting factor for producing more advanced transgenic dogs. To overcome this obstacle, we rejuvenated transgenic cells using a re-cloning technique. Fibroblasts from post-mortem red fluorescent protein (RFP) dog were reconstructed with in vivo matured oocytes and transferred into 10 surrogate dogs. One puppy was produced and confirmed as a re-cloned dog. Although the puppy was lost during birth, we successfully established a rejuvenated fibroblast cell line from this animal. The cell line was found to stably express RFP and is ready for additional genetic modification.
Hong, So Gun,Jang, Goo,Oh, Hyun Ju,Koo, Ok Jae,Park, Jung Eun,Park, Hee Jung,Kang, Sung Keun,Lee, Byeong Chun Cambridge University Press 2009 Zygote Vol.17 No.3
<B>Summary</B><P>Brain-derived neurotrophic factor (BDNF) signalling via tyrosine kinase B receptors may play an important role in ovarian development and function. It has been reported that metformin elevates the activity of Tyrosine kinase receptors and may amplify BDNF signalling. The objective of this study was to investigate the effect of BDNF during <I>in vitro</I> maturation (IVM) and/or <I>in vitro</I> culture (IVC) (Experiment 1), and to evaluate the collaborative effect of BDNF and metformin treatment on the developmental competence of bovine <I>in vitro</I> fertilized (IVF) embryos (Experiment 2). In Experiment 1, BDNF, which was added to our previously established IVM systems, significantly increased the proportions of MII oocytes at both 10 ng/ml (86.7%) and 100 ng/ml (85.4%) compared with the control (64.0%). However, there was no statistically significant difference in blastocyst development between the control or BDNF-supplemented groups. In Experiment 2, in order to investigate the effect of BDNF (10 ng/ml) and/or metformin (10<SUP>−5</SUP> M) <I>per se</I>, TCM-199 without serum and hormones was used as the control IVM medium. The BDNF (48.3%) and BDNF plus metformin (56.5%) significantly enhanced the proportions of MII oocytes compared with the control (34.4%). Although, BDNF or metformin alone had no effect in embryo development, BDNF plus metformin significantly improved early embryo development to the 8-16-cell stage compared with the control (16.5 vs. 5.5%). In conclusion, the combination of BDNF and metformin may have a collaborative effect during the IVM period. These results could further contribute to the establishment of a more efficient bovine <I>in vitro</I> embryo production system.</P>
HONG, So Gun,OH, Hyun Ju,PARK, Jung Eun,KANG, Jung Taek,KIM, Min Jung,YOON, Jung Hee,CHANG, Jin Hwa,KIM, Min Kyu,JANG, Goo,LEE, Byeong Chun Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.1
<P>This study investigated the reproductive characteristics in 2 cloned female beagle dogs (Clones A and B) during proestrus and early estrus. The pre-ovulatory estradiol peak occurred 2 days before the pre-ovulatory luteinizing hormone (LH) surge, while the follicle stimulating hormone surge started concomitantly with the LH surge in both cloned dogs. Serum progesterone levels increased during proestrus and estrus and its concentration on the day of the LH surge was 3.59 and 2.71 <I>n</I>g/m<I>l</I> in Clones A and B, respectively. Gradual follicular growth was observed by ultrasonography during proestrus. Although the numbers are limited, these cloned female dogs showed normal ranges of reproductive hormone levels and follicular changes during proestrous and early estrous stages of the cycle.</P>
Hong, So Gun,Oh, Hyun Ju,Park, Jung Eun,Kim, Min Jung,Kim, Geon A,Park, Eun Jung,Koo, Ok Jae,Kang, Sung Keun,Jang, Goo,Lee, Byeong Chun Wiley Subscription Services, Inc., A Wiley Company 2011 Genesis Vol.49 No.11
<P><B>Abstract</B></P><P>The purpose of this study was to analyze the reproductive ability of transgenic female dogs born bysomatic cell nuclear transfer and to determine inheritance of the red fluorescent protein (RFP) transgene. The four founder transgenic bitches (F0) reached puberty at 340.8 ± 39.6 days after birth and were bred with wild‐type male dogs by natural mating or by artificial insemination. The bitches all became pregnant and successfully delivered 13 puppies (F1), of which two females were bred with wild‐type dogs to deliver 7 offspring (F2), including 1 stillbirth. Among the 19 live offspring, 10 puppies showed emission of RFP under UV light and the presence of the RFP transgene was confirmed by genomic PCR and Southern blot analyses. In conclusion, transgenic RFP female dogs exhibited normal reproductive ability and expression of the transgene was demonstrated in F1 and F2 generations. genesis 49:835–840, 2011. © 2011 Wiley‐Periodicals, Inc.</P>
Hong, Heeji,Kim, So-Jeong,Min, Ui-Gi,Lee, Yong-Jae,Kim, Song-Gun,Roh, Seong Woon,Kim, Jong-Geol,Na, Jeong-Geol,Rhee, Sung-Keun International Union of Microbiological Societies 2015 International journal of systematic and evolutiona Vol.65 No.5
<P>A strictly anaerobic, mesophilic, iron-reducing bacterial strain, IRF19<SUP>T</SUP>, was isolated from coal-contaminated soil in the Republic of Korea. IRF19<SUP>T</SUP> cells were straight, rod-shaped, Gram-staining-negative and motile by means of flagella. The optimum pH and temperature for their growth were determined to be pH 7.5–8.0 and 40 °C, while the optimum range was pH 6.5–10.0 and 20–45 °C, respectively. Strain IRF19<SUP>T</SUP> did not require NaCl for growth but it tolerated up to 2 % (w/v). Growth was observed with yeast extract, <SMALL>d</SMALL>-glucose, <SMALL>d</SMALL>-fructose, <SMALL>d</SMALL>-ribose, <SMALL>d</SMALL>-mannitol, <SMALL>d</SMALL>-mannose, <SMALL>l</SMALL>-serine, <SMALL>l</SMALL>-alanine and <SMALL>l</SMALL>-isoleucine. Fe(III), elemental sulfur, thiosulfate and sulfate were used as electron acceptors. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain IRF19<SUP>T</SUP> is affiliated to the family <I>Clostridiaceae</I> and is most closely related to <I>Salimesophilobacter vulgaris</I> Zn2<SUP>T</SUP> (93.5 % similarity), <I>Geosporobacter subterraneus</I> VNs68<SUP>T</SUP> (93.2 %) and <I>Thermotalea metallivorans</I> B2-1<SUP>T</SUP> (92.3 %). The major cellular fatty acids of strain IRF19<SUP>T</SUP> were C<SUB>14 : 0</SUB>, iso-C<SUB>15 : 0</SUB> and C<SUB>16 : 0</SUB>, and the profile was distinct from those of the closely related species. The major respiratory quinone of strain IRF19<SUP>T</SUP> was menaquinone MK-5 (V-H<SUB>2</SUB>). The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown phospholipid and two unknown polar lipids. The G+C content of the genomic DNA of strain IRF19<SUP>T</SUP> was determined to be 37.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic results, strain IRF19<SUP>T</SUP> is considered to represent a novel species of a novel genus of the family <I>Clostridiaceae</I> , for which we propose the name <I>Anaerosolibacter carboniphilus</I> gen. nov., sp. nov., with the type strain IRF19<SUP>T</SUP> ( = KCTC 15396<SUP>T</SUP> = JCM 19988<SUP>T</SUP>).</P>