흉막삼출의 유무에 따른 쯔쯔가무시증 환자간의 비교

권세훈,김형호,강지인,하재하,한경택,이재록,김동민,권용은,윤성호,이승일 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Scrub typhus is a potentially fatal infectious disease caused by the organism Orientia tsutsugamushi. Clinical manifestations are fever, skin rash, eschar and varying degree of respiratory distress. The pleural effusion in scrub typhus is rare and secondary to the destruction of vascular endothelium. Because the respiratory symptoms are generally mild and the pleural effusion in scmb typhus is rare than in interstitial pneumonia, there are few comments about the characteristics of scmb typhus with pleural effusion. So we made the comparative study of scmb typhus patients between with pleural effusion and without pleural effusion. 연구배경 치명적일 수 있는 감염질환 중 하나인 쯔쯔가무시중(Scrub typhus)에서 드문 흉막삼출을 동반한 환자에 대한 고찰은 다른 문헌에서도 자주 언급되지 않았다. 따라서 본 저자들은 흉막삼출이 있는 경우와 없는 경우에 따른 쯔쯔가무시중 환자들을 비교하고자 하였다. 방법 2003년에서 2006년까지 조선대학교 병원 내과에 입원중인 환자 중 쯔쯔가무시중 진단을 받은 총 109명의 환자를 대상으로 하여 흉부 X선 검사 및 혈청학적 검사(CPK, LDH, ESR, CRP, AST, ALT, ADA), 혈액학적 검사(WBC, PLT) 및 백분율 검사등을 실시하였다. 결과 흉막삼출이 있는 환자들의 경우 흉막 삼출이 없는 환자들에 비해 ESR 수치가 통계학적으로 의의있게 높았다(p-vaule < 0.05). 결론 흉막삼출 유무에 따른 쯔쯔가무시중 환자간의 비교에 있어 ESR 수치가 통계학적인 의의를 가졌다.

Prenatal diagnosis of TRAP in the 1st trimester by transvagnial color Doppler ultrasound: A report of two cases

( Seung Taek Kim ),( Mi Hye Park ),( Kwan Young Oh ),( In Taek Hwang ),( Ji Hak Jeong ),( Jun Sook Park ) 대한산부인과학회 2001 대한산부인과학회 학술대회 Vol.87 No.-

Promising Therapeutic Strategies for Mesenchymal Stem Cell-Based Cardiovascular Regeneration: From Cell Priming to Tissue Engineering

Ji, Seung Taek,Kim, Hyunyun,Yun, Jisoo,Chung, Joo Seop,Kwon, Sang-Mo Hindawi Publishing Corporation 2017 Stem cells international Vol.2017 No.-

<P>The primary cause of death among chronic diseases worldwide is ischemic cardiovascular diseases, such as stroke and myocardial infarction. Recent evidence indicates that adult stem cell therapies involving cardiovascular regeneration represent promising strategies to treat cardiovascular diseases. Owing to their immunomodulatory properties and vascular repair capabilities, mesenchymal stem cells (MSCs) are strong candidate therapeutic stem cells for use in cardiovascular regeneration. However, major limitations must be overcome, including their very low survival rate in ischemic lesion. Various attempts have been made to improve the poor survival and longevity of engrafted MSCs. In order to develop novel therapeutic strategies, it is necessary to first identify stem cell modulators for intracellular signal triggering or niche activation. One promising therapeutic strategy is the priming of therapeutic MSCs with stem cell modulators before transplantation. Another is a tissue engineering-based therapeutic strategy involving a cell scaffold, a cell-protein-scaffold architecture made of biomaterials such as ECM or hydrogel, and cell patch- and 3D printing-based tissue engineering. This review focuses on the current clinical applications of MSCs for treating cardiovascular diseases and highlights several therapeutic strategies for promoting the therapeutic efficacy of MSCs in vitro or in vivo from cell priming to tissue engineering strategies, for use in cardiovascular regeneration.</P>

Slide Session : OS-RHEU-02 ; Rheumatology : Association of Single Nucleotide Polymorphisms of PADI4 Gene with Susceptibility to Rheumatoid Arthritis- Related Lung Disease

( Seung Taek Song ),( Song Soo Kim ),( Ji Young Kim ),( So Young Lee ),( Su Jin Yoo ),( In Seol Yoo ),( Jin Hyun Kim ),( Seong Wook Kang ),( Seung Cheol Shim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Rheumatoid arthritis (RA) causes a myriad of pulmonary complications, including bronchiolitis, bronchiectasis, pleuritis, and interstitial lung disease (ILD). Recently, several studies have shown the association of RA-related lung disease (RA-LD) with the high titers of rheumatoid factor (RF) and anti-citrullinated protein antibodies (ACPA) which are the most specifi c serologic marker for RA. Single nucleotide polymorphisms (SNPs) in a citrullinating enzyme, peptidyl arginine deiminase type IV (PADI4) have been reproducibly associated with RA susceptibility in several populations. The aim of the present study is to investigate if the SNPs in PADI4 gene are associated with RA-LD. Methods: A total of 103 consecutive RA patients, who satisfi ed the 1987 American College of Rheumatology classifi cation criteria, were genotyped for two nonsynonymous (padi4_89 and padi4_92) and one synonymous (padi4_104) SNPs in PADI4. RA-LD was diagnosed using high-resolution computed tomography of the chest. The following data were collected from medical records: Age, sex, disease duration, smoking history, use of disease-modifying anti-rheumatic drugs, RF, and ACPA. We used the independent t-test and the chi-square test. Multivariate logistic regression analysis was performed to assess the relationship between the SNPs in the PADI4 gene and RA-LD. Results: Of the 103 RA patients, 8 (7.8%) had ILD and 33 (32.0%) had small airway disease. High titers of ACPA (=80 U/mL; p=0.022) and RF (=ULN×3; p=0.008) were signifi cantly associated with susceptibility to RA-LD (Table 1). SNPs and genotypes in exon-3 (padi4_92) of PADI4 showed signifi cant association with susceptibility to RALD (p=0.013, p=0.004, respectively) (Table 2)(Table 3). Conclusions: Our results suggest that SNPs and genotypes in exon-3 (padi4_92) of PADI4 are associated with susceptibility to RA-LD. Further studies are warranted to clarify the mechanisms by which SNPs in the PADI4 gene affect the development of RA-LD.

Oleuropein attenuates hydrogen peroxide-induced autophagic cell death in human adipose-derived stem cells

Ji, Seung Taek,Kim, Yeon-Ju,Jung, Seok Yun,Kim, Da Yeon,Kang, Songhwa,Park, Ji Hye,Jang, Woong Bi,Ha, Jongseong,Yun, Jisoo,Kwon, Sang-Mo Elsevier 2018 Biochemical and biophysical research communication Vol.499 No.3

<P><B>Abstract</B></P> <P>Mesenchymal stem cells (MSCs) are multipotent progenitor cells with self-renewing properties; thus, transplanting functionally enhanced MSCs might be a promising strategy for cell therapy against ischemic diseases. However, extensive oxidative damage in ischemic tissue affects the cell fate of transplanted MSCs, eventually resulting in cell damage and autophagic cell death. Oleuropein (OLP) is a bioactive compound isolated from olives and olive oil that harbors antioxidant properties. This study aimed to investigate the potential cytoprotective effects of OLP against oxidative stress and autophagic cell death in MSCs. We found that short-term priming with OLP attenuated H<SUB>2</SUB>O<SUB>2</SUB>-induced apoptosis by regulating the pro-apoptotic marker Bax and the anti-apoptotic markers Bcl-2 and Mcl-1. Notably, OLP inhibits H<SUB>2</SUB>O<SUB>2</SUB> -induced autophagic cell death by modulating autophagy-related death signals, including mTOR (mammalian target of rapamycin), ULK1 (unc-51 like autophagy activating kinase 1), Beclin-1, AMPK (AMP-activated protein kinase), and LC3 (microtubule-associated protein 1a/1b-light chain 3). Our data suggest that OLP might reduce H<SUB>2</SUB>O<SUB>2</SUB>-induced autophagy and cell apoptosis in MSCs by regulating both the AMPK-ULK axis and the Bcl-2-Mcl-1 axis. Consequently, short-term cell priming with OLP might enhance the therapeutic effect of MSCs against ischemic vascular diseases, which provides an important potential improvement for emerging therapeutic strategies.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Oleuropein (OLP) is a bioactive compound isolated from <I>Olea europaea L.</I> </LI> <LI> Autophagy signals play an important role in cell death decisions and can protect cells by preventing apoptosis. </LI> <LI> Cytoprotective effect exerted by OLP against H<SUB>2</SUB>O<SUB>2</SUB>-induced cell apoptosis and autophagic cell death in hMSCs. </LI> </UL> </P>

Pharmacological inhibition of mTOR attenuates replicative cell senescence and improves cellular function via regulating the STAT3-PIM1 axis in human cardiac progenitor cells

Ji Hye Park,Na Kyoung Lee,Hye Ji Lim,Seung Taek Ji,김연주,Woong Bi Jang,Da Yeon Kim,강송화,Jisoo Yun,Jongseong Ha,Hyungtae Kim,Dongjun Lee,백상홍,권상모 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

The mammalian target of rapamycin (mTOR) signaling pathway efficiently regulates the energy state of cells and maintains tissue homeostasis. Dysregulation of the mTOR pathway has been implicated in several human diseases. Rapamycin is a specific inhibitor of mTOR and pharmacological inhibition of mTOR with rapamycin promote cardiac cell generation from the differentiation of mouse and human embryonic stem cells. These studies strongly implicate a role of sustained mTOR activity in the differentiating functions of embryonic stem cells; however, they do not directly address the required effect for sustained mTOR activity in human cardiac progenitor cells. In the present study, we evaluated the effect of mTOR inhibition by rapamycin on the cellular function of human cardiac progenitor cells and discovered that treatment with rapamycin markedly attenuated replicative cell senescence in human cardiac progenitor cells (hCPCs) and promoted their cellular functions. Furthermore, rapamycin not only inhibited mTOR signaling but also influenced signaling pathways, including STAT3 and PIM1, in hCPCs. Therefore, these data reveal a crucial function for rapamycin in senescent hCPCs and provide clinical strategies based on chronic mTOR activity.

Understanding on the structural and electrochemical performance of orthorhombic sodium manganese oxides

Choi, Ji Ung,Yoon, Chong Seung,Zhang, Qian,Kaghazchi, Payam,Jung, Young Hwa,Lee, Kug-Seung,Ahn, Do-Cheon,Sun, Yang-Kook,Myung, Seung-Taek The Royal Society of Chemistry 2019 Journal of materials chemistry. A, Materials for e Vol.7 No.1

<P>We investigate the orthorhombic Na0.67[NixMn1−x]O2 (<I>x</I> = 0 and 0.05) cathode materials that provide high capacity for prolonged cycles. X-ray absorption studies revealed that the redox activity of the Mn<SUP>3+/4+</SUP> and Ni<SUP>2+/3+</SUP> pairs is effective in suppressing the Jahn-Teller effect of Mn<SUP>3+</SUP> ions because of the network with Ni<SUP>2+</SUP> ions. This effect influenced the smooth voltage variations in the voltage profile for Na0.67[Ni0.05Mn0.95]O2, whereas several complicated voltage plateaus associated with the first-order phase transition were noticed in Na0.67MnO2. <I>Operando</I> synchrotron X-ray diffraction and transmission microscopy studies confirmed the simplicity of the phase transition for Na0.67[Ni0.05Mn0.95]O2 due to suppression of the Jahn-Teller effect of Mn<SUP>3+</SUP> in the oxide lattice. These findings, along with the capacity retention during prolonged cycling and the acceptable thermal properties, make high-capacity sodium-ion batteries feasible, inexpensive, and safe for energy storage application.</P>

작약(Paeoniae radix) 추출물의 식후 과혈당 억제작용

지승택(Seung-Taek Ji),이성진(Sung-Jin Lee),이강은(Kang-Eun Lee),손용태(Yong-Tae Son),정요경(Yo-Kyung Chung) 한국식품영양과학회 2002 한국식품영양과학회지 Vol.31 No.1

본 연구에서는 작약 추출물의 식후 과혈당 억제작용을 조사하였다. 작약 유기용매(hexane, ethyl acetate, butanol, water)추출물들을 high performance liquid chromatography를 이용하여 분획한 분획물(fraction)들의 α-glucosidase(EC 3. 2. 1. 20) 저해제를 탐색하였다. 작약 ethyl acetate 추출물의 11, 12, 18, 19번 분획물들은 20 μg/mL에서 각각 85%, 84%, 77%, 77%의 강력한 저해 활성을 나타내었으며, 작약 ethyl acetate 추출물의 10~19번 분획을 경구투여한 in vivo당(maltose) 부하 실험에서도 양성대조군과 비교하여 유의성 있게 22%의 혈당을 낮추었다. 따라서 본 연구의 in vitro와 in vivo 실험을 통하여 확인 된 α-glucosidase 저해 활성을 갖는 작약 분획물들은 식후의 혈당상승 작용을 억제하는 새로운 nutraceutical 소재와 항당뇨 신약개발을 위한 탐색자원으로서 매우 가치있는 자원으로 기대된다. This study was carried out to investigate the inhibitory effect of extracts from Paeoniae radix on postprandial hyperglycemia. Organic solvent (hexane, ethyl acetate, butanol, water) extracts from Paeoniae radix were fractionated by high performance liquid chromatography. These fractions were used to screen α-glucosidase (EC 3. 2. 1. 20) inhibitors by microplate colorimetric assay. The fractions 11, 12, 18, 19 of ethyl acetate extract from Paeoniae radix showed inhibitory activity by 85%, 84%, 77%, 77% at concentration of 20 μg/mL, respectively. The selected fractions (no. 10~no. 19) significantly reduced by 22% the blood glucose elevation in comparison with positive control in mice loaded with maltose. The fractions of Paeoniae radix were determined in vitro inhibitory activity on α-glucosidase and in vivo inhibition effect on blood glucose elevation in mice. Therefore, these results suggest that the extract of Paeoniae radix can be used as a new nutraceutial for inhibition on postprandial hyperglycemia as well as resource pool for lead compounds as a α-glucosidase inhibitor.

KOH를 이용한 N-face GaN의 습식 식각으로 인한 표면 변화

김택승 ( Taek Seung Kim ),한승철 ( Seung Cheol Han ),김재관 ( Jae Kwan Kim ),이지면 ( Ji Myon Lee ) 대한금속재료학회 2008 대한금속·재료학회지 Vol.46 No.4

Acute Pulmonary Toxicity and Body Distribution of Inhaled Metallic Silver Nanoparticles

Jung-Taek Kwon,Arash Minai-Tehrani,Soon-Kyung Hwang,Ji-Eun Kim,Ji-Young Shin,Kyeong-Nam Yu,Seung-Hee Chang,Dae-Seong Kim,Yong-Taek Kwon,In-Ja Choi,Yun-Hee Cheong,Jun Sung Kim,Myung-Haing Cho 한국독성학회 2012 Toxicological Research Vol.28 No.1

The purpose of this study was to determine the acute pulmonary toxicity of metallic silver nanoparticles (MSNPs, 20.30 ㎚ in diameter). Acute pulmonary toxicity and body distribution of inhaled MSNPs in mice were evaluated using a nose-only exposure chamber (NOEC) system. Bronchoalveolar lavage (BAL) fluid analysis, Western blotting, histopathological changes, and silver burdens in various organs were determined in mice. Mice were exposed to MSNPs for 6 hrs. The mean concentration, total surface area, volume and mass concentrations in the NOEC were maintained at 1.93 × 10? particles/㎤, 1.09 × 10<SUP>10</SUP> ㎚²/㎤, 2.72 × 10<SUP>11</SUP> ㎚³/㎤, and 2854.62 ㎍/㎥, respectively. Inhalation of MSPNs caused mild pulmonary toxicity with distribution of silver in various organs but the silver burdens decreased rapidly at 24-hrs post-exposure in the lung. Furthermore, inhaled MSNPs induced activation of mitogen-activated protein kinase (MAPK) signaling in the lung. In summary, single inhaled MSNPs caused mild pulmonary toxicity, which was associated with activated MAPK signaling. Taken together, our results suggest that the inhalation toxicity of MSNPs should be carefully considered at the molecular level.