과열증기처리 반탄화 추출물의 항산화 효과 검증에 관한 연구

오근혜(Geun Hye Oh),남정빈(Jeong Bin Nam),양승민(Seung Min Yang),정원희(Won Hee Joung),정진산(Jin San Jeong),신종민(Jong Min Shin),강석구(Seog Goo Kang) 한국펄프·종이공학회 2018 펄프.종이기술 Vol.50 No.2

The antioxidant properties of the extracts from torrefied wood subjected to superheated steam treatment were investigated. Total polyphenolics, total flavonoids, DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity, reducing power, and FRAP (Ferric reducing antioxidant power) were determined to evaluate antioxidant activity. The total polyphenol content was 879.67±40.41 ㎎/mL at a concentration of 25 g/mL when the extract was subjected to a temperature of 300℃ for 10 min, and 759.67±25.17 ㎎/mL at a concentration of 25 g/mL at 350℃ for 10 min. The value of at 300℃ was 15.79% higher than that obtained at 350℃. Total flavonoid content was 111.18±3.55 ㎎/mL at a concentration of 25 g/mL at 300℃ for 10 min, and 80.58±2.58 ㎎/mL at a concentration of 25 g/mL at 350℃ for 10 min. The value at 300℃ was 37.97% higher than that obtained at 350℃. For the DPPH free radical scavenging activity, the highest scavenging activity was observed at a concentration of 6 g/mL. DPPH free radical scavenging activity at a concentration of 6 g/mL at 300℃ for 10 min was 89.83±0.03%, whereas at 350℃ for 10 min at the same concentration, it was found to be 87.99±0.1%. The value at 300℃ was 2.09% higher than that obtained at 350℃. Reducing power was determined to be 3.59±0.04 at a concentration of 25 g/mL at 300℃ for 10 min, and 2.92±0.1 at a concentration of 25 g/mL at 350℃ for 10 min. The value at 300℃ was 22.94% higher than that obtained at 350℃. FRAP was 1742±37 μM at a concentration of 25 g/mL at 300℃ for 10 min, and 1106±8 μM at a at 350℃ for 10 min. At 300℃, the FRAP value was 57.50% higher than that obtained at 350℃. Based on these results, we suggest that torrefied wood treated with superheated steam can be used in various applications because of its effective antioxidant properties.

배지의 종류와 부피가 절화 장미 ‘Red Sandra’와 ‘Little Marble’ 삽목시 발근 및 생장에 미치는 영향

정종운(Jong Woon Jeong),김경희(Gyeong Hee Kim),황승재(Seung Jae Hwang),박수민(Su Min Park),정병룡(Byoung Ryong Jeong) (사)한국생물환경조절학회 2007 생물환경조절학회지 Vol.16 No.4

본 연구는 배지의 종류 및 부피가 절화 장미의 발근과 발근 후 생육에 미치는 영향을 알아보기 위하여 수행하였다. 실험은 유리온실의 철재 베드에 높이 1.5m의 아치형 터널을 설치하고 습도유지를 위하여 fog(200sec./5min. or 100sec./5min) 가습하고, 삽목 초기에 차광률 55%의 한랭살 차광하였다. 실험재료는 Rosa hybrida ‘Red Sandra’와 ‘Little Mable’ 두 품종을 사용하였다. 배지의 종류에 따른 발근 차이를 알아보기 위하여 10가지 배지(토실이 상토, 암면큐브, peatmoss 1:1, perlite와 coir 1;1, perlite와 rockwool 1;1, rockwool과 peatmoss 1;1, rockwool과 coir 1;1, v/v)를 준비하여 삽목하였다. 발근배지의 부피에 따른 발근 및 생육의 차이를 알아보기 위하여 3가지의 배지(토실이 상토, 암면큐브, phenolic foam)를 각각 3가지 부피(3×3×3㎝, 5×5×5㎝, 7×7×7㎝)에 삽목하였다. 배지의 종류를 달리한 실험은 두 품종 모두에서 Rockwool l: Peatmoss l 배지에서 발근과 생육에서 가장 좋은 결과가 나타났으며, 배지의 부피에 따라 발근율과 모든 생육은 배지의 용적이 커질수록 좋았다. Experiments were conducted to measure rooting and growth of rose cuttings as affected by medium composition and volume. Rosa hybrid a ‘Red Sandra’ and ‘Little Marble’ were used as test plants. For medium composition test, rockwool, phenolic foam, perlite, vermiculite, peatmoss, coir and a commercial (Tosilee medium) were applied in single or mixed forms on the propagation bed with fog (200 sec./5 min.) installed to control the humidification time. ‘Red Sandra’ and ‘Little Marble’ cuttings were planted in 16-cell plastic pots and 72-cell plug trays, respectively. For the medium volume test, 72-cell plug trays and 16-cell or 9-cell plastic pots were used. Cuttings with an uniform node with a five-leaflet leaf was soaked in a 500 ㎎ㆍ L ?¹ IBA solution for 30 sec. A fogging system installed on the propagation bed was used for (100 sec./5 min.) Rockwool l : peatmoss l (v/v) was the most effective on growth and rooting for both cultivars and high volume of Tosilee medium and rockwool were most effective media for both cultivars.

배지의 종류와 부피가 절화 장미 ‘Red Sandra’와 ‘Little Marble’ 삽목시 발근 및 생장에 미치는 영향

정종운(Jong Woon Jeong),김경희(Gyeong Hee Kim),황승재(Seung Jae Hwang),박수민(Su Min Park),정병룡(Byoung Ryong Jeong) (사)한국생물환경조절학회 2005 생물환경조절학회지 Vol.14 No.2

본 연구는 배지의 종류 및 부피가 절화 장미의 발근과 발근 후 생육에 미치는 영향을 알아보기 위하여 수행하였다. 실험은 유리온실의 철재 베드에 높이 1.5m의 아치형 터널을 설치하고 습도유지를 위하여 fog(200sec./5min. or 100sec./5min) 가습하고, 삽목초기에 차광률 55%의 한랭사로 차광하였다. 실험재료는 Rosa hybrida ‘Red Sandra’와 ‘Little Marble' 두 품종을 사용하였다. 배지의 종류에 따른 발근 차이를 알아보기 위하여 10가지 배지(토실이 상토, 암면큐브, phenolic foam, perlite, vermiculite, 또는 perlite와 peatmoss 1 : 1, perlite와 coir 1 : 1, perlite와 rockwool 1 : 1, rockwool과 peatmoss 1 : 1, rockwool과 coir 1 : 1, v/v)를 준비하여 삽목하였다. 발근배지의 부피에 따른 발근 및 생육의 차이를 알아보기 위하여 3가지의 배지(토실이 상토, 암면큐브, phenolic foam)를 각각 3가지 부피(3 × 3 × 3cm, 5 × 5 × 5cm, 7 × 7 × 7cm)에 삽목하였다. 배지의 종류를 달리한 실험은 두 품종 모두에서 Rockwool 1: Peatmoss 1 배지에서 발근과 생육에서 가장 좋은 결과가 나타났으며, 배지의 부피에 따른 발근율과 모든 생육은 배지의 용적이 커질수록 좋았다. Experiments were conducted to measure rooting and growth of rose cuttings as affected by medium composition and volume. Rosa hybrid a 'Red Sandra' and 'Little Marble' were used as test plants. For medium composition test, rockwool, phenolic foam, perlite, vermiculite, peatmoss, coir and a commercial medium (Tosilee medium) were applied in single or mixed forms on the propagation bed with fog (200 sec./5 min.) installed to control the humidification time. 'Red Sandra' and 'Little Marble' cuttings were planted in 16-cell plastic pots and 72-cell plug trays, respectively. For the medium volume test, 72-cell plug trays and 16-cell or 9-cell plastic pots were used. Cuttings with an uniform node with a five-leaflet leaf was soaked in a 500 ㎎ㆍL?¹ IBA solution for 30 sec. A fogging system installed on the propagation bed was used for (100 sec./5 min.). Rockwool 1 : peatmoss 1 (v/v) was the most effective on growth and rooting for both cultivars and high volume of Tosilee medium and rockwool were most effective media for both cultivars.

Effects of hydrogen plasma treatment on SnO2:F substrates for amorphous Si thin film solar cells

Min-Seung Choi,Young-Ju Lee,Jung-Dae Kwon,Yongsoo Jeong,Ju-Yun Park,강용철,Pung Keun Song,Dong-Ho Kim 한국물리학회 2013 Current Applied Physics Vol.13 No.8

We investigated the effects of hydrogen plasma treatment on the physical and electrical properties of fluorine-doped tin oxide (FTO) films used for amorphous silicon (a-Si) thin film solar cells. A slight increase in carrier concentration by the hydrogen doping effect was observed for the FTO film exposed to the hydrogen plasma for 5 min. For further exposure to the plasma, the chemical reduction became prominent and resulted in deterioration of the electrical and optical properties of the film. XPS analysis revealed that the chemical reduction of SnO2 to Sn metallic state occurs on the surface region. It was found that the defects formed by hydrogen plasma act as recombination centers at the interface between FTO electrode and p-layer of a-Si solar cells. This phenomenon resulted in the deterioration of the cell performance. The averaged conversion efficiency (6.82%) of the cells on pristine FTO hydrogen substrate was decreased to 5.81% for the cells on FTO treated for 5 min, which is mainly attributed to the decrease in short-circuit current density.

소부혈(少府穴) 자침(刺鍼)이 Kainic Acid로 유도(誘導)된 간질(癎疾) 동물(動物)모델의 해마(海馬) 치상회(齒狀回)에 미치는 영향(影響)

김승태 ( Seung Tae Kim ),정주호 ( Joo Ho Chung ),정우병 ( Wu Byung Jeong ),김장현 ( Jang Hyun Kim ),강민정 ( Min Jung Kang ),홍미숙 ( Mee Sook Hong ),박해정 ( Hae Jeong Park ),김연정 ( Yeon Jung Kim ),박히준 ( Hi Joon Park ),이혜 대한경락경혈학회 2007 Korean Journal of Acupuncture Vol.24 No.4

Objectives: Epilepsy is one of the most common serious brain disorders that affect people of all ages, and it is characterized by recurrent unprovoked seizures. We examined whether acupuncture can reduce both the incidence of seizures and hippocampal cell death in dentate gyrus (DG) using a mouse model of kainic acid (KA)-induced epilepsy. Methods: ICR mice (20~25g) were given acupuncture once a day at acupoint HT8 (sobu) bilaterally during 2 days before KA injection. After an intracerebroventricular injection of 0.1μg of KA, acupuncture treatment was subsequently administered once more (total 3 times), and the degree of seizure was observed for 20 min. Three hours after injection, we confirmed the neural cell death using cresyl violet staining and silver impregnation staining, and determined the expressions of c-Fos and glutamate decarboxylase (GAD)-67 using immunohistochemistry techniques in the DG. Results: KA induced epileptic seizure, neural cell death, increased c-Fos expression and decreased GAD-67 expression in the DG. Acupuncture treatment at HT8 reduced the severity of the epileptic seizure and inhibited neural cell death from KA. In addition, acupuncture normalized the expressions of c-Fos and GAD-67 in the same areas. Conclusions: These results demonstrated that acupuncture treatment at HT8 may reduce the KA-induced epileptic seizure and neural cell death in the DG possibly by normalizing c-Fos expressions and the gamma-aminobutyric acid neurons.

Pseudomonas sp. BCNU 171균에서의 유기용매 shock반응과 heat shock반응의 연관성

이승범,김미림,정미연,박정욱,김민정,주우홍 Institute of Genetic Engineering Changwon National 1999 Gene and Protein Vol.3 No.1

The response of Pseudomonas sp. BCNU 171 to various forms of stress was examined by using O'Farrell two-dimensional gel electrophoresis. The toluene resistance of mid-growth cells of Pseudomonas sp. BCNU 171 grown at 30℃ was enhanced by exposing the cells to a prior toluene shock in the presence of 0.05% toluene for 30 min. The heat tolerance of the cell was also induced by the same condition. Individual protein expression was examined at 30 min and 60 min by silver staining after the addition of 0.05%(v/v) toluene to mid-growing cultures of the strain BCNU 171. The toluene stress response increased the expression of eighteen proteins between 30 and 60 min toluene exposure. At 60 min after toluene addition, a toluene specific protein was detected. A few proteins induced by toluene and heat were overlapped on 2D-PAGE.

헬스케어용 가다랑어(Katsuwonus pelamis) 통조림의 제조 및 특성

김현정 ( Hyeon Jeong Kim ),김민지 ( Min Ji Kim ),김기현 ( Ki Hyun Kim ),지성준 ( Seung Jun Ji ),임경훈 ( Kyung Hun Lim ),박권현 ( Kwon Hyun Park ),신준호 ( Joon Ho Shin ),허민수 ( Min Soo Heu ),김진수 ( Jin Soo Kim ) 한국수산과학회 2012 한국수산과학회지 Vol.45 No.3

This study was conducted to prepare canned skipjack tuna Katsuwonus pelamis in the mixture of isotonic beverage and tomato paste (ST-S) for use as a health food. An analysis of the time-temperature profile and viable cells showed that a reasonable F0 value for ST-S preparation was 4 min. The proximate composition of ST-S was 76.8% moisture, 20.2% crude protein, 0.8% crude lipid, 1.7% ash and 0.5% carbohydrate. The calorie content of ST-S was 94.8 kcal, which is 47.4% lower than that of commercial canned skipjack tuna in oil (ST-O) and 2.2% lower than that of commercial chicken breast in water (CB-B). The total amino acid content of ST-S was 18.54 g/100 g, which is 31.4% lower than that of ST-O and 7.9% lower than that of CB-B. The major amino acids in ST-S were aspartic acid and glutamic acid. An enrichment effect due to such minerals as phosphorus, potassium and iron would be expected on consuming 100 g of ST-S. The major fatty acids in ST-S were 16:0 (27.4%), 18:1n-9 (14.3%) and 22:6n-3 (27.8%), which are different from those in ST-O and CB-B. The major free amino acids in ST-S were glutamic acid (8.1%), histidine (38.6%) and its related dipeptide, such as anserine (15.7%). In an evaluation of taste, flavor and color, ST-S was found to be superior to ST-O and CB-B.

Antagonizing effect of protein kinase C activation on the μ-opioid agonist-induced inhibition of high voltage-activated calcium current in rat periaqueductal gray neuron

Cho, Young-Wuk,Han, Seung-Ho,Min, Byung-Il,Rhee, Jeong-Seop,Norio Akaike 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2001 No.-

Opioids have been thought to induce analgesia by activating the descending pain control system, especially at the level of periaquenductal gray, and regulate the neurotransmitter release through the inhibition of calcium channel. In the present study, the modulatory effects of protein kinase C and protein kinase A on the μ-opioid agonist-induced inhibition of the high-voltage activated calcium current were examined in the acutely dissociated rat periaqueductal gray neurons with the nystatin-perforated patch-clamp technique. Among 505 neurons tested, the barium current passing through the high-voltage activated calcium channels of 172 neurons (34%) were inhibited by 32±3% with the application of an μ-opioid agonist, [D-_Ala^2, N-MePhe^4, Gly^5 -ol]-enkephalin (DAMGO, 1 μM). The barium currents itself and the DAMGO-induced inhibitory effects were not affected by the application of either an adenylate cyclase activator (forskolin, 1μM) or a protein kinase inhibitor (staurosporin, 10nM) for 2 min. The DAMGO inhibition was completely and irreversibly antagonized by the application of a protein kinase C activator, phorbol-12-myristate-13-acetate (PMA, 1μM) for 2 min without any alteration of the barium current itself. However, the antagonizing effect of PMA was completely abolished by the application of 10 nM staurosporin for 2 min. After then, PMA did not show the antagonizing effect any more. Inversely, when staurosporin was applied before PMA, the antagonizing effect of PMA was also not shown. These results demonstrate that the μ-opiod agonist-induced inhibition of the periaqueductal gray neuronal high-voltage activated calcium current can be antagonized by protein kinase C activation. This finding may provide us a significant clue to understand the action mechanism of opioid-induced analgesia in the periaqueductal gray. ⓒ 2001 Elsevier Science B.V.All rights reserved.

뇌경색후 발생한 Cortical Spreading Depression과 뇌경색 범위에 관한 실험적 연구

박윤관,이승민,김세훈,이기찬,주정화,이훈갑 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.3

Cortical Spreading Depression(CSD) is a transient depression of neuronal activity that spreads across the cortical surface and is associated with profound changes in blood flow, extracellular ion concentration. Direct Current(DC) potentials and cell membrane potentials. One of the electrophysiological disturbance in the periinfarct surrounding is spontaneous occurrence of repeated CSD like DC shifts associated with increased energy demand. Due to restricted blood flow to the periinfarct border zone, elevated metabolic demand is potentially harzardous. So the authors designed this experiment to verify the correlation between periinfarct cortical spreading depression and ischemic volume following permanent middle cerebral artery (MCA) occlusion in rats. Sprague-Dawley rats(n=27) were anesthetized with 0.5∼1% halothane, and artificially ventilated through a tracheal cannula. Arterial pressure, blood gases and body temperature were controlled. The middle cerebral artery(MCA) was occluded distally to the lenticulostriate branches. Measurements of CSD activity were made for 4 hours in each animal. Infarct volume was determined 6 hours later in 2, 3, 5-triphenyl tetrazolium chloride(TTC)-stained sections. For 4 hours after MCA occlusion, the CSDs were found in all experimental animals with a range of 2∼9 times. Those CSDs wer of varying duration, : "small"(<1 min) and "big"(> 1 min) SDs and mean of total duration of SD was 10.5±10.3 min during 4 hours of MCA occlusion. Neuropathological evaluation of brain infarct in the rats, which had been allowed to survive for 6 hours after MCA occlusion showed a mean volume of 89.7±45.3㎣. Serial observation of duration of CSD showed progressive prolongation only in 8 rats. The rest of them(n=19) did not show any prolongation. Neither the prolongation of duration of CSD nor the frequency of CSD in the penumbral zone correlated with the volume of infarct. However total duration of CSD was slightly related with the infarct volume after 6 hours of the permanent MCA occlusion(r=0.414, p=0.0318).

Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase

Song, Jae-Young,Choi, Yeo-Jeong,Kim, Jeong-Min,Kim, Yoo-Ree,Jo, Jin-Seong,Park, Jin-Sik,Park, Hee-Jin,Song, Yun-Gyu,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Youn, Hee-Shang,Cho, Myung-Je,Rhee, Kw The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.4

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and 21 kDa, respectively. N-terminal amino acid sequence of heavy and light subunits revealed that H. pylori GGT was processed into 3 parts for a signal peptide of 27 amino acid residues, a heavy subunit of 352 residues, and a light subunit of 188 residues during translation. The reaction rate for hydrolysis of ${\gamma}$-GpNA was 84.4 ${\mu}mol/min$ per milligram of protein, and that for the ${\gamma}$-glutamyl transfer from ${\gamma}$-GpNA to gly-gly was 23.8 ${\mu}mol/min$ per milligram of protein. The apparent Km values of H. pylori GGT for ${\gamma}$-glutamyl compounds were on the order of $10^{-3}$ to $10^{-4}$ M and those for acceptor peptides and amino acids were on the order of $10^{-1}$ to $10^{-2}$ M. The GGT protein kept approximately 80% of the initial enzymatic activity on incubation at $60^{\circ}C$ for 15 min. The optimum temperature and pH for reactions of both hydrolysis and transpeptidation were $40^{\circ}C$ and 9.0, respectively. The transpeptidation and hydrolysis reactions catalyzed by H. pylori GGT were strongly inhibited by L-Gln and moderately inhibited by L-Ala, L-Ser, ${\beta}$-chloro-L-Ala, and L-Glu. These results demonstrated that the biochemical properties of H. pylori GGT are different from those of other bacterial GGTs. Further, H. pylori GGT might degrade glutathione in the gastric mucous layer of humans if the enzyme could be secreted in the bacterial niches.