Protective Effect of Heme Oxygenase-1 on High Glucose-Induced Pancreatic β-Cell Injury

Lee, Eun-Mi,Lee, Young-Eun,Lee, Esder,Ryu, Gyeong Ryul,Ko, Seung-Hyun,Moon, Sung-Dae,Song, Ki-Ho,Ahn, Yu-Bae Korean Diabetes Association 2011 Diabetes and Metabolism Journal Vol.35 No.5

<P><B>Background</B></P><P>Glucose toxicity that is caused by chronic exposure to a high glucose concentration leads to islet dysfunction and induces apoptosis in pancreatic β-cells. Heme oxygenase-1 (HO-1) has been identified as an anti-apoptotic and cytoprotective gene. The purpose of this study is to investigate whether HO-1 up-regulation when using metalloprotophyrin (cobalt protoporphyrin, CoPP) could protect pancreatic β-cells from high glucose-induced apoptosis.</P><P><B>Methods</B></P><P>Reverse transcription-polymerase chain reaction was performed to analyze the CoPP-induced mRNA expression of HO-1. Cell viability of INS-1 cells cultured in the presence of CoPP was examined by acridine orange/propidium iodide staining. The generation of intracellular reactive oxygen species (ROS) was measured using flow cytometry. Glucose stimulated insulin secretion (GSIS) was determined following incubation with CoPP in different glucose concentrations.</P><P><B>Results</B></P><P>CoPP increased HO-1 mRNA expression in both a dose- and time-dependent manner. Overexpression of HO-1 inhibited caspase-3, and the number of dead cells in the presence of CoPP was significantly decreased when exposed to high glucose conditions (HG). CoPP also decreased the generation of intracellular ROS by 50% during 72 hours of culture with HG. However, decreased GSIS was not recovered even in the presence of CoPP.</P><P><B>Conclusion</B></P><P>Our data suggest that CoPP-induced HO-1 up-regulation results in protection from high glucose-induced apoptosis in INS-1 cells; however, glucose stimulated insulin secretion is not restored.</P>

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

조기 능동 운동을 가능케 하는 굴건 수복 방법에 관한 생체외 연구

안상로,윤승호 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

The results of the flexor tendon repair within the digital sheath have been unpredictable due to peritendinous adhesion. It has been known that three factors are responsible for the formation of adhesion, that is, tendon sheath injury, tendon suture and immobilization. Of these three factors, immobilization is the only one that is theoretically under the control of the surgeon. This problem has led to the development of early active and protective mobilization techniques. However, there was no method providing enough repair strength to a point sufficient to permit early active flexion without risk of tendon rupture or large gap formation. Also the protective mobilization techniques produced a gap at the repair site and could not provide normal amplitude of tendon excursion. So, the author performed these four in vitro studies to obtain a method of flexor tendon repair permitting early active flexion against moderate resistance. The deduced tensile strength permitting early active flexion against moderate resistance is above 7.5kg. The extensor digiti quarti proprius tendon of adult pigs' foreleg was used as the experimental tendon. Each tendon repair was performed under magnification by the Meiji experimental opera tive microscope. The tensile strength in each experiment was tested with a Tensilon tensometer and the gap was measured at each kg. of tensile strength. The first experiment was the measurement of the tensile strength of two strands of 4-0 mersilene with one knot and six strands of 4-0 mersilene with three knots. The second experiment was the measurement of the tensile strength of the diagonal grasp from one grasp to four grasps with a knot, in the tendon end and with a knot far from the tendon end. The third experiment was the measurement of the tensile strength and gap formation of the author's method. Three interrupted sutures with. knots far from the tendon ends were placed in the tendon. Two sutures were placed in both posterolateral aspects of the tendon and one suture was placed in the anterior aspect of the tendon. Each suture grasped only one-sixth of the circumference of the tendon and was placed periph-erally to preserve the intratendinous vasculature. The fourth experiment was the measurement of the tensile strength and gap formation of modified Kessler's, Ketchum's and Savage's method. The following results were obtained. 1. The tensile strength of two strands of 4-0 mersilene with one knot and six srands of 4-0 mersilene with three knots was 2. 45kg and 7.20 kg. respectively which was similar to the deduced tensile strength of 2.5kg. and 7.5kg. respectively. 2. The tensile strength of three and four diagonal grasps with a knot far from the tendon end was 2.83kg. and 2.96kg. respectively which was above the deduced tensile strength of 2.5kg. 3. The tensile strength of the author's method was 8.04kg. which was above the deduced tensile strength of 7.5kg. The gap formation of the author's method with each kg. of tension was low as follows, 0 mm. at 1 kg., 0. 03mm. at 2 kg., 0. 16mm. at 3 kg. 0. 71 mm. at 4 kg. , 1. 53mm. at 5 kg., 2. 38mm. at 6 kg., 3.61mm. at 7 kg., 4.75mm. at 8 kg.. 4. The tensile strength of various methods was low and the gap formation of various methods. was large compared to the author's method. This author's method provided a high tensile strength and a minimal gap, so this method will. be one of the methods that permit immediate active finger flexion against moderate resistance even after tendon softening.

뇌졸중 환자의 경직 감소를 위한 경피적 전기 신경 자극술과 침술의 효과

백천호,소성욱,김희상,안경회,남상수,박선구 大韓再活醫學會 1997 Annals of Rehabilitation Medicine Vol.21 No.6

6세 이하의 어린이에서 Streptococcus mutans와 Streptococcus sobrinus의 분포에 관한 연구

안승태,박재홍,이긍호 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.2

Mutans streptococci는 치아우식증과 관련이 있다고 보고 되고 있으며, 이 streptococci 종 중에서 S. mutans나 S. sobrinus가 인간의 치아우식증에서 가장 일반적으로 나타난다. 치아우식증에서 이 세균종의 분포는 인종과 지역에 따라 다양하다. 본 연구에서는 2-6세인 52명의 치아우식증 환자에서 비우식부위와 우식부위로부터 치태를 채취하여 DNA를 추출한 dex와 gtf 유전자에 기초한 S. mutana와 S. sobrinus에 특이한 primer를 제작한 후 PCR을 시행하여 S. mutans와 S. sobrinus의 분포도를 조사하여 다음과 같은 결과를 얻었다. 1. 어린이 치아우식증 환자에서 S. mutans나 S. sobrinus가 발견된 어린이는 37명 (71.2%)이었다. 2. 이들 어린이 중에서, S. mutans 또는 S. sobrinus가 비우식 부위에서만 발견된 어린이는 3명(5.8%), 우식 부위에서만 발전된 어린이는 22명(42.3%), 두 부위 모두에서 발견된 어린이는 12명(23.1%)이었다. 3. S. mustans나 S. sobrinus가 나타난 비우식부위 치태는 15개 (28.8%), 우식부위 치태는 34개(65.4%)였다. 4. 비우식부위의 경우 S. mutans만 출현한 어린이는 52명 중 8명(15.4%), S. sobrinus만 출현한 어린이는 6명(11.5%), 두 세균종이 같이 출현한 어린이는 1명 (1.9%)이었다. 5. 우식부위에서 S. mutans 출현한 어린이는 52명 중 24명(46.2%), S. sobrinus만 출현한 어린이는 2명(3.8%), 두 세균종이 같이 출현한 어린이는 8명 (15.4%)이었다. 6. S. mutans는 gtf에 기초한 primer, S. sobrinus는 dex에 기초한 primer가 PCR에서 효과적으로 각각의 세균을 검출하였다. 이상의 결과로 미루어 우리나라 어린이의 경우 치아우식증은 MS와 밀접한 연관성이 있으며, MS중에서도 S. mutans가 중요하다고 판단된다. 한편 S. mutans와 S. Sobrinus의 동반출현은 치아우식증과 더욱 밀접한 관련이 있는 것으로 보인다. Mutans streptococci have been reported to be implicated in dental caries. Of these streptococcal species, Streptcoccus mutans and Streptococcus sobrinus are most commonly found in human dental caries. Prevalence of these bacterial species in dental caries is found to be varied in different races and countries. Therefore, importance of these bacteria in dental caries remains to be determined. The present study was performed to detect S. mutans and S. sobrinus in 52 Korean children with dental caries between 2 to 6 years of age. For the study, plaque samples were collected from caries-active(CA) and caries-free(CF) teeth of each subject. DNA was extracted from the plaques and amplified by polymerase chain reaction(PCR) using primers corresponding to dex and gtf genes. The results obtained from the study were as follows: 1. Of 52 children, 37 children (71.2%) were found to harbor S. mutans and/or S. sobrinus. 2. Of the MS-infected 37 children, 3 children (5.8%) harbored S. mutans and/or S. sobrinus in the CF plaques only, 22 children (42.3%) in the CA plaques only and 12 children (23.1%) in both CF and CA plaques. 3. S. mutans and/or S. sobrinus were detected in 34 CA plaques (65.4%), while 15 in CF plaques (28.8%). 4. In the case of CF plaques, 8 plaques (15.4%) were observed to harbor S. mutans only, 6 plaques (11.5%) to harbor S. sobrinus only and 1 plaque (1.9%) to harbor both species. 5. Of CA plaques, 24 plaques (46.2%) were detected to have S. mutans only, 2 plaques (3.8%) to have S. sobrinus only and 8 plaques (15.4%) to have both species. 6. In comparison with the efficiency of two different primers for PCR, it was found that the primer based on gtf gene was more effective in detecting S. mutans, while the primer corresponding to dex gene was better for S. sobrinus. Overall results suggest that MS appears to be important in dental caries of the Korean children, and S. mutans is more closely associated with than dental caries as compared to S. sobrinus.

P2P 모델에 기반한 혼합형 파일 검색 알고리즘의 설계

백승재,안진호 京機大學校 2006 論文集 Vol.50 No.-

Recently, peer-to-peer model is more preferable to client-server model in developing file sharing systems because of desirable features of the first such as scalability, anonymity, security, fault-tolerance and so on. However, the most well-known P2P file sharing systems Napster and Gnutella result in some scalability problems, centralized control and broadcasting in file searching, respectively. To address the problems, ultrapeers and dynamic routing techniques were proposed but, cannot still avoid incurring long response times and low recall rates of rare files. This paper presents a hybrid strategy to use existing Gnutella-like techniques for locating popular files and DHT-like techniques for searching rare ones. In particular, we propose an efficient DHT algorithm to structure a node ring consisting of only ultrapeers, not all the nodes. This feature makes the algorithm more scalable than the previous DHT ones in terms of the costs of file search, node join and leave operations and the number of routing table entries each node should maintain.

최대 오차 벡터를 이용한 최적 공간 벡터 변조 기법에 관한 연구

안호균,김태엽,진승오,남징락 國立 昌原大學校 産業技術硏究所 1998 産技硏論文集 Vol.12 No.-

This study proposes a optimized space vector modulation(SVM), which makes a good output current with low total harmonic distortion(THD) and introduces the concept of maximum error vector for new method. The performance of the new modulation technique using maximum error vector is shown by simulation and experiments.

한국사람 손등피부신경의 분포 양상

안동춘,황규성,백두진,한승호,정호삼 대한체질인류학회 1999 해부·생물인류학 (Anat Biol Anthropol) Vol.12 No.1

한국사람 손등피부신경의 분포양상을 알아보기 위해 59구(남자 39, 여자 20)의 시체에서 113예(오른손 58/ 왼손 55)의 손등을 해부하여 관찰하였다. 분포유형은 노선경의 분포영역을 기준으로 자신경과의 분포 범위에 따라 구분하여 다음과 같은 결과를 얻었다. 1. 노신경과 자신경으로 이루어진 손등피부신경의 분포양상은 10가지 유형이 관찰되었다. 2. 노신경이 셋째손가락 노쪽절반까지, 자신경이 셋째손가락 자쪽절반까지 이르는 경우(제VIII형)가 33.9%로 가장 많이 관찰되었다. 3. 노신경과 자신경이 셋째손샅에서 혼재하는 경우로, 노신경이 넷째손가락 노쪽절반까지, 자신경이 셋째손가락 자쪽절반까지 이르는 경우(제III형)가 25.7%, 노신경이 셋째손가락까지, 자신경이 셋째손가락 자쪽절반까지 이르는 경우 (제VI형)가 11.0%였다. 4. 노신경과 자신경의 등쪽손가락신경이 셋째손샅에서 합쳐져 하나의 신경가지를 이루는 경우(제IV형)가 12.8%로 나타났다. 5. 두 신경이 둘째손샅과 셋째손샅에서 혼재하거나 둘째손샅에서 합쳐져 분포하는 제III, VI, IX, X, XI 형은 새롭게 관찰된 유형이었다. 6. 근육피부신경이 노신경을 대신하였던 경우는 4가지 유형으로 각각 1예씩 관찰되었다. 7. 손등피부신경의 분포양상을 성별 및 왼손과 오른손에 따라 비교한 결과 통계학적으로 유의한 차이는 없었다. 이상의 결과로 보아 한국인의 손등피부신경은 노신경과 자신경이 셋째손가락 양쪽까지 이르는 경우가 가장 흔한 유형임을 알 수 있었다. In order to investigate the distribution patterns of the dorsal digital nerves of the radial and ulnar nerve in the Korean, authors dissect the 113 hands (right 58/left 55) of the 59 cadavers(39 males/20 females). The types were classified by the area of radial dorsal digital nerves and the ulnar digital nerves. The difference in the distribution pattern between males and females, right and left hands was analysed by x^(2)-test in the case presenting the prequency more than 10%. The results as follows; I. The ten types of the distribution patterns consisted of the radial and ulnar nerves were observed on the dorsum of the hands. 2. The case of the highest prequency was type VIII(33.9%), in which radial nerve supply the radial side of the 2½ of digits and ulnar nerve extends the ulnar side 2½ of digits. 3. In the cases of the both nerve mingling in the third digital web, the incidences in which the radial nerves extend to the radial half of ring finger and ulnar nerve to the ulnar half of middle finger(type III) were 25.7%, and that the radial nerves extend to the ulnar half of middle finger and ulnar nerve to the ulnar half of middle finger(type VI) were 11.0%. 4. Type IV as combined branch between the radial and the ulnar nerve extend to the third digital web was observed in the 12.8%. 5. The type III, VI, IX, X, XI showing the both nerves mingling in the third digital web and in the second digital web or combining in the second digital web were new observed in the Korean. 6. The musculocutaneous nerve replaces the superficial branch of the radial nerve in 4 cases. 7. There was no statistical difference in the distribution pattern between males and females, right and left hands. From the above results, it was suggested that the majority of the cases were that the ulnar digital nerves supplied the ulnar half of the middle finger in the Korean.

최소차 하천의 임계면적에 따른 유역 지형매개변수의 변화특성

안승섭,박노삼,김종호,임기석,송시훈 한국환경과학회 2005 한국환경과학회지 Vol.14 No.1

The size of minimum order stream has a very sensitive effect on runoff analysis model using the divergence characteristic of stream. Therefore, in this study, the threshold area of minimum order stream has been examined the change characteristic of topographical parameters. The subject basin of the research was the upper basin of the Kumho water gage station which is located in the middle of the Kumho river. The 1:25,000 numerical geography which was constructed 10×lOm mesh was used. The range of investigation of topographical parameters are number of stream order, length, area, slope, basin relief, sinuosity ratio, drainage density and total stream length etc. It was found from the result of analysis that the threshold value of minimum order stream has a very big effect on topographical parameters of basin. It was found that the threshold area of minimum ord er stream revealed under 0.10km². Furthermore, the parameters showed a serious change except for over 0.10km².

HEC-RAS/GIS를 이용한 홍수 범람지역 분석

안승섭,이증석,김종호 한국환경과학회 2004 한국환경과학회지 Vol.13 No.1

The purpose of the study was to construct a forecast system of flood inundation area at natural stream channels. The study built the system to interpret the flood inundation area in four stages ; constructing topography data around the stream channel, interpreting flood discharge, interpreting flood elevation in the stream channel, and interpreting the flood inundation and mapping. According to the result of the analysis, as for the characteristic of flood inundation around the area within the purview of this study, although there were areas where flood inundation over a bank caused a flooded area, the failure of the internal drainage in the ground lower than flood elevation caused more serious problems. Rather than the existing method where only the estimated flood elevation data is used based on the hydrographical stream channel trace model(such as the HEC-RAS model) to establish the flood inundation area, if the procedure introduced in this study was applied to interpret the floodplain, actual flood inundation area could be visibly confirmed.