Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy.

Park, Sungwoo,Choi, Seon-Guk,Yoo, Seung-Min,Son, Jin H,Jung, Yong-Keun Landes Bioscience 2014 AUTOPHAGY Vol.10 No.11

<P>CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and SN4741 dopaminergic neuronal cells. Conversely, overexpression of CHDH accelerates PARK2-mediated mitophagy. CHDH is found on both the outer and inner membranes of mitochondria in resting cells. Interestingly, upon induction of mitophagy, CHDH accumulates on the outer membrane in a mitochondrial potential-dependent manner. We found that CHDH is not a substrate of PARK2 but interacts with SQSTM1 independently of PARK2 to recruit SQSTM1 into depolarized mitochondria. The FB1 domain of CHDH is exposed to the cytosol and is required for the interaction with SQSTM1, and overexpression of the FB1 domain only in cytosol reduces CCCP-induced mitochondrial degradation via competitive interaction with SQSTM1. In addition, CHDH, but not the CHDH FB1 deletion mutant, forms a ternary protein complex with SQSTM1 and MAP1LC3 (LC3), leading to loading of LC3 onto the damaged mitochondria via SQSTM1. Further, CHDH is crucial to the mitophagy induced by MPP+ in SN4741 cells. Overall, our results suggest that CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition.</P>

만성중이염의 골도청력변화

박선홍,노용훈,이도용,나한조,김용기,최봉남,도남용 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.1

We have usually experienced bone conduction impairment in chronic otitis media, but it is unclear that raised bone conduction threshold is due to the reflection of cochlear dam- age and / or Carhart effect ( an artificial elevation of bone conduction threshold due to conductive defect ) or both. The purpose of this study is to verify the influence of the artificial elevation of the bone conduction threshold in chronic otitis media and to estimate Carhart effect. A total of 336 cases of tympanoplasty with unilateral chronic otitis media without any other causes of sensorineural hearing loss was studied. The average threshold levels of the bone conduction in diseased ears ranged from 18.5dB at 4kHz to 12.1dB at 250Hz The longer duration of the disease, the larger perforation size, the more severe damage of the middle ear structure and the presence of the cholesteatoma made bone conduction threshold increased. The postoperative thresholds of bone conduction were Improved in 36 cases (40.4%) of the 89 cases that underwent operation with type I tympanoplasty technique without electrie drilling. The degree of the maximal improvement was 14.3dB at 2000Hz and the minimum was 8.8dB at 250Hz. but these result did differ from a statistically significant degree. The result suggest that the bone conduction threshold in chronic otitis media is elevated artificially by conductive defect and improved by successful reconstructive surgery.

비·부비동 종양의 신생혈관 형성에서 COX-2 및 VEGF의 역할

도남용,박성용,조성일,윤혁수,박선홍,박영균,권대승,임성철 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1

Background and Objectives : COX-2, the inducible form of cyclooxygenase(COX), is upregulated in transformed cells and in malignant cells, which have important roles in promotion of colorectal carcinogenesis, invasiveness and angiogenesis. Vascular endothelial growth factor(VEGF) may act as endothelial cell mitogen in various cancer tissues. It will increase tumor growth and angiogenesis. Aims of this study were to asses COX-2/VEGF expression and it's clinical correlation in sinonasal tumors and to investigate the effects on angiogenesis also. Materials and Method : The study samples were obtained from surgical removal of 27 cases of inverted papilloma(IPs), 5 cases of IP with malignant transformation(IP-MT), 16 cases of Squamous cell carcinoma(SCC) in sinonasal cavity COX-2 and VEGF expressions were assessed by immunohistochemical staining. Synchronism of COX-2/VEGF expression in one tumor cell was demonstrated with double immunofluorescence technique. Results : The expression rates of COX-2 were 37% in IP, 80% in IP-MT and 100% in SCC. The positive rates of VEGF were 29 6% in IP, 100% in IP-MT and SCC. COX-2 and VEGF expressions were increased as tumor progressed, and there is a positive correlation between COX-2 &EGF expressions. All clinical features which were compared are not associated with COX-2 &EGF expressions except malignant change in IP(p=0 028). Synchronism of COX-2/VEGF expressions was noted in all positive immunostaining samples. Conclusion : These findings suggest the role of COX-2 pathway could be involved in sinonasal tumor angiogenesis, which is modulated by VEGF COX-2 may act as autocrine factor in VEGF expression. There is a potential role for selective COX-2 inhibitors in the treatment of these lesions.

신경섬유종증 환자에서 상완신경총 병변의 소견을 보인 척추 신경섬유종

양충용,박효인,박순아,박종태,정선관,신용일 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

본 증례는 50세 여성의 제 1형 신경섬유종 환자로서 척추 신경섬유종에 의해 초기 상완신경총 병변 소견을 보였다. 약 4개월 동안 후경부통, 좌측 견관절, 좌측 상완의 마비를 보였으며, 초기 전기진단학적 검사상 경수 5번과 6번 신경근을 중심으로 쇄골상부 신경절전성 상완신경총 병변이 관찰되었다. 그러나, 액와 자기공명영상, 초음파검사, 단순 방사선검사, 골주사 등의 영상학적 검사에서는 심한 흉추 척추 전-측만증과 경수 뇌경막에 확장 소견을 제외하고는 상완신경총 병변의 소견은 관찰되지 않았다. 7개월경과 후, 좌측 상지 및 양측 하지의 근력 저하, 경수 4번이하의 감각 저하, 경도의 흉통을 동반한 호흡곤란이 진행되었다. 응급으로 시행된 경수 자기공명영상에서 좌측 경수 3번과 4번에 경막내 액와-속질의 신경종양이 진단되었다. 수술적 처치를 시행하였으며, 거시적 관찰상 척수 신경섬유종이 진단되었다. 급격히 진행하는 척수 신경섬유종은 초기에 상완신경총의 신경근 병변의 소견을 보일 수 있으므로, 신경섬유종증 환자에서 상완신경총 병변의 진단에 세심한 주의를 요한다. We observed a patient with symptom similar to brachial plexus lesion caused by spinal neurofibroma in a 50-year-old female with a history of neurofibromatosis-1(NF-1). She presented with posterior neck, left shoulder and left upper arm paralysis of 4 months duration. The initial electrodiagnostic study(EDS) revealed supraclavicular preganglionic brachial plexus lesion of mainly involved left C5 and C6 roots. However, radiological imagings including axillar MRI, ultrasonography, cervical plain radiography and bone scintigraphy showed no definitive brachial plexus disease except cervical dural dilation and severe thoracic scolio-Iordosis. On 7 months after the first admission, she had aggravated muscle weakness on the left upper arm and both lower extremities, sensory impairment below C4 and respiration difficulty with the chest pain. Emergency MRI showed intradural axillar-medullary neurogenic tumor at the left C3-C4. Subsequently, the patient was treated with surgical resection. Grossly specimen examination displayed the presence of a spinal neurofibroma. In rapidly progressing spinal neurofibroma represented firstly as root lesion of brachial plexus in EDS, the physician should pay scrupulous care to evaluate the cause.

활성화 수피를 이용한 중금속 흡착제 개발

박창진,양재의,유경열,장용선,김원일 한국환경농학회 2004 한국환경농학회지 Vol.23 No.4

The objective of this research was to develop the adsorbent for heavy metals by activating the bark sample. Barks from pine tree with diameters of 2~4 mm were activated in the muffle furnace under a high relative humidity condition at temperatures of 600~900℃. The removal efficiency of the activated bark (ACTBARK) for Cu and Cd was temperature dependent showing the order of 900℃ > 800℃ > 700℃ > 600℃. The critical temperature was considered to be 900℃ to become an efficient adsorbent for Cu and Cd. The bark samples activated at temperatures lower than 700℃ showed a less removal efficiency than the crude bark The ACTBARK activated at 900℃ removed more Cu and Cd from solution than the commercial activated carbon and charcoal. The ACTBARK (activated at 900℃) adsorbed all of the Cu and Cd in solution with concentrations less than 150 mg/L. The selectivity of the ACTBARK was in the order of Cu>Zn>Ni>Pb>Fe>Cd>Mn.

NDE 및 생체자기 계측을 위한 SQUID 응용

박용기,김인선,이용호,권혁찬,김진목,김기웅 한국비파괴검사학회 2003 학술대회 논문집 Vol.- No.1

SQUID is the most sensitive magnetic sensor known. It is able to detect the magnetic field as small as few femto Tesla (fT), which is equivalent to one of 10 billionth of earth magnetic field (about 50 ??T). SQUID can be applied in the various fields and its need will be increased greatly in the future. If SQUID is applied in the medical technology, there will be improvement in accurate diagnosis of brain and heart by supplying more information about the functions of such human organs. The nondestructive evaluation (NDE) of deep-lying flaws in the inner layer of lab jointed structures of aircraft, which is very difficult with the existing method became possible using high T_c SQUID. In this paper, the current status of SQUID applications in both medical and NDE fields will be reviewed.

이온교환막을 통한 전해질 용액의 이동 특성

박형관,한정우,선용호,조영일 연세대학교 산업기술연구소 1989 논문집 Vol.21 No.1

The transport characteristics of electrolyte osmosis through the perfluorinated cation exchange membrane, Nafion NX-430, was inverstigated. The order of electrolyte permeation velocities of single valent ions was:??>??>??>??. At constant concentration ratio the electrolyte fluxes have shown maximum and minimum values with respect to electrolyte concentration, which is called anomalus osmosis. The correlations between potassium ion fluxes and concentration ratios was shown as follows. ?? The effective charge density calclated from membrane potential was 1% of the fixed charge concentration.

노인의 Helicobacter pylori 재균율

박주상,홍원선,이영수,이은주,명승재,정훈용,양석균,김진호,민영일 대한노인병학회 2000 Annals of geriatric medicine and research Vol.4 No.3

성경에 나타난 도 (道)

박선철,박용천 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.4

Objectives : It is interesting that the term "Tao" is seen in the Bible of Korean revised version, because Tao is the essential part of traditional Korean culture. Korean culture has 5000 years of history rooted in the humanitarian ideal that means devotion to the welfare of mankind. Such humanitarian ideal had accepted, digested and finally recreated many religions and ideas without conflict. Because understanding the patient's culture is important in the psychiatric practice, it is worthwhile to compare the Tao in the Bible and in the traditional culture. This will lead us to understand the Bible more accurately, and Un-derstanding the Bible can broaden our view toward the traditional Korean culture. Methods : The authors searched the term "Tao" in the Bible ofKorean Revised Version and compared it with the Biblia Hebrica Stuttgatensia, and Novum Testatum Graece to detect the original expressions of Tao. The meaning of Hebrew or Greek original expressions were compared with English expressions of The Holy Bible, the King James Version. Results : Tao was recorded 85 times in the Bible of Korean Revised Version. The majority of original expressions were "derek" (43 times) in Hebrew Old Testaments, and "hodos" (14 times) in Greek New Testament. The majority of English expressions corresponding to Tao were "way" or "ways" (59 times). "Derek" means the commandments of God. "Hodos" means the way ofJesus Christ. Conclusion : "Derek" and "hodos" commonly mean the righteousness and sanctification required to human with the berith (testament) of God. Tao in the Bible ofKorean Revised Version is similar to Tao in the traditional culture in the viewpoint of enlightenment. Korean psychotherapy can be elaborated further with the acceptance of Westem psychotherapy on the basis of deep understanding of Korean traditional culture, such as Tao.

품종 및 배지의 종류가 양액재배 무화과의 생장과 발육에 미치는 영향

박순기,이범선,박용서,정순주 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

무화과의 암면 양액재배를 통하여 시설재배의 가능성을 검토하고 배지종류 및 품종에 따른 과실생육 및 수량을 검토하여 우리나라 기후에 가장 알맞는 품종을 선발하고 육성을 도모하기 위하여 수행하였다. 봉래시 품종을 4종류의 배지(펄라이트, 코코피트, 암면슬라브 및 입상암면)에 재식하고, 또한 '마쓰이도후인', '봉래시', '화이트제노아' 및 '브라운터어키' 품종을 본엽 4∼5매인 5월 17일에 암면슬라브에 각각 15주씩 정식하여 생장반응과 총수량 등을 조사한바 배지종류에 따른 봉래시 품종의 생장반응은 암면과 입상암면 배지에서 초장, 엽수, 엽장 및 측지수가 다른 배지보다 높게 나타나 이들 두가지 배지의 이용 가능성이 컸고, 암면을 사용하여 재배한 경우 초기 생장은 봉래시 품종이 좋았으나 과실의 수량면에서 단위면적당 총수량과 주당 평균수량은 마쓰이도후인>브라운터어키>봉래시, 화이트제노아 순으로 많았고, 과실 한개당 평균무게, 과장 및 과폭은 브라운터어키 품종이 가장 높았다. 우리나라 남부지역에서 시설내 양액재배에 가장 적당한 품종은 마쓰이도후인 품종과 브라운터어키 품종으로 추천되었다. This experiment was carried out to know the effect of cultivars and substrates on the growth and development of fig tree (Ficus carica L.) grown in hydroponics. Four cultivars ; Masui Dauphine, Bongraeshi, White Genoa and Brown Turkey, were used and four substrates, perlite, cocopeat, rockwool and granular rockwool were employed. Every cultivars cutted into 20cm with two buds on 27th, of February and then inserted into fockwool cube for root initiation, Every fifteen cuttings were transplanted on 17th of May in each substrate. Among cultivars 'Bongraeshi' showed highest growth in terms of plant height, number of leaves and shoots, and leaf length in the both rockwool and granular rockwool. Total yields per square meter and average yields per plant were higher in the order of Masui Dauphine > Brown Turkey > Bongraesh and White Genoa. Brown Turkey showed the heavier frjuit weight and larger fruit length and width than those of the other cultivars. Consequently, optimum cultivars and substradtes for hydroponics were recommended as Masui Dauphine and Brown Turkey, and rockwool and granular rockwool. However further trials would be followed as to the nutrient concentration, composition and solution managemint for improving fruit yield and quality of fig tree during growing period.