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김기영,박샛별,문지혜,이상대,이새롬,장윤정 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2
This study was performed to develop a rapid test kit for pathogenic Staphylococcus in various samples. The rapid detection kit has been fabricated based on nitrocellulose lateral-flow strip. Colloidal gold and Staphylococcus antibodies were used as a tag and a receptor, respectively. Manually spotted Staphylococcus antibody and anti-mouse antibody on the surface of nitrocellulose membrane were used as test and control lines, espectively. Feasibility of the rapid kit to detect Staphylococcus aureus in samples were evaluated. The intensity of the color of the tes line started to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was 106 cfu/mL Staphylococcus spiked in PBS. Also, the rapid test kit could detect 105 cfu/mL of Staphylococcus in chicken meat extract.
Evaluation of Anti-allergy and Anti-oxidative Effects of Green tea Cultivar Extracts
Saet Byeol Park(박샛별),Doo Gyung Moon(문두경),Hyung Don Kim(김형돈),Je Hun Choi(최재훈),Kyung Hae Soe(서경혜),Gwi Yeong Jang(장귀영),Jeong Hoon Lee(이정훈),Dong Hwi Kim(김동휘),Seung Eun Lee(이승은) 한국약용작물학회 2018 한국약용작물학회 학술대회논문집 Vol.2018 No.2
Multi-user Precoding-aided Spatial Modulation with Block Diagonalizaion
Saet-Byeol Lee(이샛별),Manar Mohaisen(마나르 모하이센) 대한전자공학회 2016 대한전자공학회 학술대회 Vol.2016 No.6
In this paper, we introduce a multi-user precoding-aided spatial modulation based on block diagonalization (BD). In contrast to conventional algorithms, our proposed technique eliminates the inter-user interference (IUI) though applying BD. As such, the proposed system avoids the inversion of either large-size matrices or a set of matrices, as compared to conventional techniques. In terms of system performance, the proposed technique can be used in under-determined and determined systems, with considerable improvements of the system performance in case of the under-determined systems due to the increased diversity gain.
Isolation of Early Neurogenesis Genes with Xenopus cDNA Microarray
Saet-Byeol Yu,Ok-Joo Lee,Young-Ju Park,Sung-Young Lee,Seung-Hwan Lee,Jaeho Yoon,Yoo-Seok Hwang,Jong-Il Kim,Jae-Yong Lee,Jae-Bong Park,Sung Chan Kim,Jaebong Kim 한국실험동물학회 2010 Laboratory Animal Research Vol.26 No.1
Neurogenesis is the process that develops neuroectoderm from ectoderm. Bone morphogenetic protein (BMP) inhibition in ectodermal cells is necessary and sufficient for neurogenesis in Xenopus embryos. To isolate genes involved in early neurogenesis, Xenous Affymetrix gene chips representing 14,400 genes were analyzed in early stage of neuroectodermal cells that were produced by inhibition of BMP signaling with overexpression of a dominant-negative receptor. We identified 265 candidate genes including 107 ESTs which were newly expressed during the early neurogenesis by blocking BMP signaling. The candidates of 10 ESTs were selected and examined for upregulation in neuroectoderm. Five EST genes were confirmed to be upregulated in neuroectoderm and examined for time-dependent expression patterns in intact embryos. Two EST genes were cloned and identified as a homology of CYP26c (Xl.1946.1.A1_at) and Kielin containing VWC domain (Xl.15853.1.A1_at). One of them, CYP26c, was further characterized for its transcriptional regulation and role of anterior-posterior patterning during neurogenesis. Taken together, we analyzed and characterized genes expressed in early neurogenesis. The results suggest that neurogenesis by inhibition of BMP provides useful system to isolate genes involved in early events of neurogenesis during early vertebrate embryogenesis.
Altered PLCβ-1 expression in the gerbil hippocampal complex following spontaneous seizure
( Saet Byeol Lee ),( Yun Jung Oh ),( Jae Kwang Chung ),( Ji Heon Jeong ),( Sang Duk Lee ),( Dae Kyoon Park ),( Kyung Ho Park ),( Jeong Sik Ko ),( Duk Soo Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.9
Although the phospholipase C (PLC)β-1 isoform is associated with spontaneous seizure and distinctively expressed in the telencephalon, the distribution of PLCβ-1 expression in the epileptic gerbil hippocampus remains controversial. Therefore, we determined whether PLCβ-1 is associated with spontaneous seizure in an animal model of genetic epilepsy. In the present study, PLCβ-1 immunoreactivity was down-regulated in seizure-sensitive (SS) gerbils more than in seizure-resistant (SR) gerbils. The expression of PLCβ-1 within calretinin (CR)-positive neurons was rarely detected within the dentate hilar region of SS gerbils. PLCβ-1 immunoreactivity in the hippocampus was significantly elevated as compared to that in pre-seizure SS gerbil 3 h post-ictal. These findings suggest that alterations in PLCβ-1 immunoreactivity in the SS gerbil hippocampus may be closely related to the epileptic state of the gerbil brain and transiently elevated PLCβ-1 protein levels following seizure episodes. Such alterations may be compensatory responses in the SS gerbil hippocampus. [BMB reports 2011; 44(9) : 566-571]
Yu, Saet-Byeol,Umair, Zobia,Kumar, Shiv,Lee, Unjoo,Lee, Seung-Hwan,Kim, Jong-Il,Kim, SungChan,Park, Jae-Bong,Lee, Jae-Yong,Kim, Jaebong Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.4
Vertebrate neurogenesis requires inhibition of endogenous bone morphogenetic protein (BMP) signals in the ectoderm. Blocking of BMPs in animal cap explants causes the formation of anterior neural tissues as a default fate. To identify genes involved in the anterior neural specification, we analyzed gene expression profiles using a Xenopus Affymetrix Gene Chip after BMP-4 inhibition in animal cap explants. We found that the xCyp26c gene, encoding a retinoic acid (RA) degradation enzyme, was upregulated following inhibition of BMP signaling in early neuroectodermal cells. Whole-mount in situ hybridization analysis showed that xCyp26c expression started in the anterior region during the early neurula stage. Overexpression of xCyp26c weakly induced neural genes in animal cap explants. xCyp26c abolished the expression of all trans-/cis-RA-induced posterior genes, but not basic FGF-induced posterior genes. Depletion of xCyp26c by morpholino-oligonucleotides suppressed the normal formation of the axis and head, indicating that xCyp26c plays a critical role in the specification of anterior neural tissue in whole embryos. In animal cap explants, however, xCyp26c morpholinos did not alter anterior-to-posterior neural tissue formation. Together, these results suggest that xCyp26c plays a specific role in anterior-posterior (A-P) neural patterning of Xenopus embryos.
초등학생의 소프트웨어교육을 위한 디자인사고 기반 메이커교육 프로그램 적용
이샛별(Saet-Byeol Lee),이승철(Seung-Chul Lee),김태영(Tae-Young Kim) 한국컴퓨터교육학회 2018 한국컴퓨터교육학회 학술발표대회논문집 Vol.22 No.2
미래사회가 요구하는 창의융합형 인재 육성을 위한 기초소양 함양을 위해 소프트웨어 교육이 의무화되는 등 컴퓨팅 사고력과 지능정보사회에 필요한 핵심역량인 협력적 문제해결력의 향상을 위한 소프트웨어 교육에서의 교수·학습 방법이 강조되고 있다. 이에 따라 본 연구에서는 감성적 사고와 창의적 사고력의 균형을 추구하는 디자인 사고와 실생활 맥락적 문제를 해결하는 메이커 활동을 결합한 디자인 사고 기반 메이커교육을 소프트웨어 교육에 적용하여 초등학생의 협력적 문제해결력에 미치는 영향을 검증하고자 한다. 이를 통하여 디자인 사고기반 메이커 교육의 학습효과를 밝히고, 초등학교 수업에서 다른 교과목과 융합한 소프트웨어 교육의 적용방법에 대한 시사점을 제공하고자 한다.
초등학생의 소프트웨어교육을 위한 메이커교육 프로그램 적용 및 효과성 분석
이샛별 ( Saet-byeol Lee ),이승철 ( Seung-chul Lee ),김태영 ( Tae-young Kim ) 한국컴퓨터교육학회 2018 한국컴퓨터교육학회 학술발표대회논문집 Vol.23 No.1
미래사회가 요구하는 창의융합형 인재 육성을 위한 기초소양 함양을 위해 소프트웨어 교육이 의무화되는 등 컴퓨팅 사고력과 지능정보사회에 필요한 21세기 창의적 인재의 핵심역량 함양을 위한 소프트웨어 교육에서의 교수·학습 방법이 강조되고 있다. 이에 따라 본 연구에서는 수업환경과 방식의 변화에 따라 실생활 맥락적 문제를 해결하는 메이커 활동을 소프트웨어 교육에 적용하여 새로운 교육 방향의 필요성에 따른 교수·학습 방법을 제시하고 학습자의 반응을 분석하여 메이커 교육의 효과성 및 앞으로의 소프트웨어 교육의 방향성에 대한 시사점을 제공하고자 한다.