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Yu, Saet-Byeol,Umair, Zobia,Kumar, Shiv,Lee, Unjoo,Lee, Seung-Hwan,Kim, Jong-Il,Kim, SungChan,Park, Jae-Bong,Lee, Jae-Yong,Kim, Jaebong Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.4
Vertebrate neurogenesis requires inhibition of endogenous bone morphogenetic protein (BMP) signals in the ectoderm. Blocking of BMPs in animal cap explants causes the formation of anterior neural tissues as a default fate. To identify genes involved in the anterior neural specification, we analyzed gene expression profiles using a Xenopus Affymetrix Gene Chip after BMP-4 inhibition in animal cap explants. We found that the xCyp26c gene, encoding a retinoic acid (RA) degradation enzyme, was upregulated following inhibition of BMP signaling in early neuroectodermal cells. Whole-mount in situ hybridization analysis showed that xCyp26c expression started in the anterior region during the early neurula stage. Overexpression of xCyp26c weakly induced neural genes in animal cap explants. xCyp26c abolished the expression of all trans-/cis-RA-induced posterior genes, but not basic FGF-induced posterior genes. Depletion of xCyp26c by morpholino-oligonucleotides suppressed the normal formation of the axis and head, indicating that xCyp26c plays a critical role in the specification of anterior neural tissue in whole embryos. In animal cap explants, however, xCyp26c morpholinos did not alter anterior-to-posterior neural tissue formation. Together, these results suggest that xCyp26c plays a specific role in anterior-posterior (A-P) neural patterning of Xenopus embryos.
Isolation of Early Neurogenesis Genes with Xenopus cDNA Microarray
Saet-Byeol Yu,Ok-Joo Lee,Young-Ju Park,Sung-Young Lee,Seung-Hwan Lee,Jaeho Yoon,Yoo-Seok Hwang,Jong-Il Kim,Jae-Yong Lee,Jae-Bong Park,Sung Chan Kim,Jaebong Kim 한국실험동물학회 2010 Laboratory Animal Research Vol.26 No.1
Neurogenesis is the process that develops neuroectoderm from ectoderm. Bone morphogenetic protein (BMP) inhibition in ectodermal cells is necessary and sufficient for neurogenesis in Xenopus embryos. To isolate genes involved in early neurogenesis, Xenous Affymetrix gene chips representing 14,400 genes were analyzed in early stage of neuroectodermal cells that were produced by inhibition of BMP signaling with overexpression of a dominant-negative receptor. We identified 265 candidate genes including 107 ESTs which were newly expressed during the early neurogenesis by blocking BMP signaling. The candidates of 10 ESTs were selected and examined for upregulation in neuroectoderm. Five EST genes were confirmed to be upregulated in neuroectoderm and examined for time-dependent expression patterns in intact embryos. Two EST genes were cloned and identified as a homology of CYP26c (Xl.1946.1.A1_at) and Kielin containing VWC domain (Xl.15853.1.A1_at). One of them, CYP26c, was further characterized for its transcriptional regulation and role of anterior-posterior patterning during neurogenesis. Taken together, we analyzed and characterized genes expressed in early neurogenesis. The results suggest that neurogenesis by inhibition of BMP provides useful system to isolate genes involved in early events of neurogenesis during early vertebrate embryogenesis.
ATSC Mobile DTV에서 적용 가능한 재난방송 프로토콜 설계 및 수신기법
유샛별 ( Saet-byeol Yu ),조민주 ( Min-ju Cho ),황준 ( Jun Hwang ) 한국인터넷정보학회 2011 인터넷정보학회논문지 Vol.12 No.6
본 논문에서는 ATSC(Advanced Television System Committee) Mobile DTV에서 신뢰성이 보장되고 빠른 시간 안에 재난정보가 수신될 수 있도록 하는 재난방송 시스템을 제안한다. 미국에서 현재 사용되고 있는 Cable용 재난 표준인 EAM(Emergency Alert Message)을 기반으로 프로토콜을 정의 하였으며, 가장 신속하게 재난정보가 전달 될 수 있도록 성능측정을 하여 최적의 방법을 제안하였다. 기존 미국식 방송규격인 ATSC legacy 시스템에 영향을 전혀 미치지 않으며 모바일 방송을 전송할 수 있도록 하는 ATSC Mobile DTV는, 전원에 구애받지 않고 재난방송을 전달할 수 있는 효과적인 매체라는 장점과 함께 미국 전역뿐만 아니라 ATSC규격을 채택한 다양한 국가에서 적용이 가능하다. In this paper, a fast and reliable emergency broadcasting system for Advanced Television System Committee (ATSC) Mobile DTV is proposed. The proposed protocol is based on the Emergency Alert Message (EAM) standard currently used for cable TV emergency broadcasting in the United States. The protocol is implemented and evaluated to enable fast emergency information propagation. ATSC Mobile DTV enables digital mobile broadcasting without affecting the existing ATSC legacy digital TV system. Since ATSC Mobile DTV devices are mobile and self-powered, they can effectively propagate emergency information. The proposed emergency broadcasting protocol can be applied in all countries adopting the ATSC standard.
안정적인 ATSC Mobile DTV 방송을 위한 모니터링 시스템 설계 및 구현
유샛별(Saet-Byeol Yu),조민주(Min-Ju Cho),손예진(Ye-Jin Sohn),전성희(Sung-Hee Chon),이재호(Jae-Ho Lee) 한국컴퓨터정보학회 2010 韓國컴퓨터情報學會論文誌 Vol.15 No.8
디지털 방송의 경우 아날로그 방송과 달리 다양한 변수를 포함하고 있으며 디지털의 특성상 에러가 발생할 경우 수신이 전혀 되지 않을 수 있다. 그래서 디지털 방송에는 다양한 변수들을 고려하여 에러에 대응할 수 있도록 모니터링 시스템을 운영하고 있다. ATSC Mobile DTV는 기존의 주파수를 그대로 사용하면서 Mobile 방송을 함께 제공하는 시스템으로, 기존의 시스템과의 간섭이나 영향이 발생 할 수 있기 때문에 실시간 방송상황을 모니터링 하고 에러 발생 시 이를 관리자에게 알려줄 수 있는 모니터링 프로그램이 요구된다. 이에 본 논문에서는 ATSC Mobile DTV 실시간 방송 상태를 감시하고, 수신감도가 좋지 않거나 동영상 스트림 오류등과 같은 문제가 발생 했을 시 이를 관리자에게 알려 안정된 디지털 방송시스템을 유지할 수 있는 효율적인 모니터링 시스템을 제안한다. Digital broadcasting system has various affecting factors unlike analog broadcasting system, and has possibility of no signal reception in case of errors due to characteristics of digital system. And, digital broadcasting system has monitoring system for error occurrences handling various factors. ATSC mobile DTV system uses legacy frequency and provides mobile broadcasting services, and also requires monitoring systems for monitoring online broadcasting services and reporting to administrator about interference or influence with legacy systems, and error situations. In this paper, we propose efficient monitoring system which monitors online broadcasting status of ATSC mobile DTV, reports on poor signal reception, video streaming failures to administrator, and maintains reliable digital broad ca sting system.